SIST EN 46-1:2016
(Main)Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method)
Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method)
This European Standard specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: - water-insoluble chemicals which are being studied as active insecticides; - organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; - organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and - water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1: Anwendung durch Oberflächenverfahren (Laboratoriumsverfahren)
Diese Europäische Norm legt ein Verfahren zur Bestimmung der vorbeugenden Wirkung eines Holzschutzmittels gegenüber frisch geschlüpften Larven (Eilarven) von Hylotrupes bajulus (Linnaeus) fest, wobei das Holzschutzmittel zur Oberflächenbehandlung des Holzes verwendet wird.
Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
- wasserlösliche Stoffe, zum Beispiel Salze.
Das Verfahren gilt unabhängig davon, ob die Prüfkörper einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.
Produits de préservation des bois - Détermination de l'action préventice contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 1 : Application par traitement de surfave (Méthode de laboratoire)
La présente Norme européenne prescrit une méthode de détermination de l’action préventive d'un produit de préservation du bois contre les larves récemment écloses d’Hylotrupes bajulus (Linnaeus) lorsque le produit de préservation est appliqué au bois par un traitement de surface.
La présente méthode est applicable :
- aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides ;
- aux formules organiques telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ;
- aux formules organiques hydrodispersables telles qu'elles sont fournies ou préparées en laboratoire par dilution de concentrés ; et
- aux produits solubles dans l’eau, des sels par exemple.
Cette méthode s’applique à des éprouvettes ayant subi ou non des épreuves de vieillissement appropriées.
Biocidni proizvodi za zaščito lesa - Ugotavljanje preventivnega delovanja proti hišnemu kozličku Hylotrupes bajulus (Linnaeus) - 1. del: Ugotavljanje učinkovitosti proti ličinkam (laboratorijska metoda)
Ta evropski standard določa metodo za ugotavljanje preventivnega delovanja zaščitnega
sredstva za les proti hišnemu kozličku Hylotrupes bajulus (Linnaeus), ko je zaščitno sredstvo
uporabljeno v sklopu površinske obdelave lesa.
Ta metoda se uporablja za:
– v vodi netopne kemikalije, ki se preučujejo kot aktivni insekticidi;
– organske formulacije, ki so dobavljene ali pripravljene v laboratoriju z redčenjem koncentratov;
– organske formulacije, ki se razpršijo v vodi in so dobavljene ali pripravljene v laboratoriju z redčenjem
koncentratov ter
– v vodi topne materiale, na primer sol.
Metoda se uporablja ne glede na to, ali so preskušanci izpostavljeni ustreznim postopkom staranja ali ne.
General Information
Relations
Overview
EN 46-1:2016 (CEN) - "Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method)" specifies a laboratory procedure to assess the preventive (larvicidal) action of surface-applied wood preservatives against newly hatched Hylotrupes bajulus larvae. The method is applicable to water‑soluble, water‑insoluble and organic (including water‑dispersible) formulations and can be used with test specimens that have or have not undergone ageing procedures.
Key topics and technical requirements
- Biological material: use recently hatched Hylotrupes bajulus larvae (within 3 days of hatching); 10 larvae per test specimen collected from eggs laid by different females.
- Product types covered: water‑insoluble active chemicals, organic formulations (as supplied or diluted), water‑dispersible organics and water‑soluble materials (e.g., salts).
- Specimen preparation:
- Species and quality of wood are harmonized; dimensions and number of specimens are specified.
- End faces sealed depending on treatment medium: paraffin wax for aqueous systems (setting point ~52–54 °C recommended) and gelatin for treatments with organic solvents.
- Treatment vessels must be non‑reactive (e.g., glass for organic products; polyethylene for fluorinated salts).
- Conditioning & test environments:
- Culturing chamber: (28 ± 2) °C, RH (70 ± 5) %.
- Conditioning chamber: (20 ± 2) °C, RH (65 ± 5) %.
- Testing chamber: (22 ± 2) °C, RH (70 ± 5) %.
- Safety & handling:
- Avoid hazardous solvents (explicit caution against benzene); use separated work areas, extraction and PPE.
- Annex D provides laboratory environmental, health and safety guidance.
- Validation and reporting:
- Examination of attack and expression of results with criteria for test validity; 2016 edition adds option to use X‑ray for validity checks.
- Annexes include culturing methods, wood sapwood/heartwood differentiation and procedures for slow‑acting formulations.
Applications and users
- Practical uses:
- Evaluate whether surface treatments prevent recently hatched Hylotrupes bajulus larvae from penetrating treated wood and surviving in untreated zones.
- Compare larvicidal performance of different formulations, concentrations or aged vs. unaged specimens.
- Support product development, registration, certification and specification of wood preservatives.
- Typical users:
- Accredited biological testing laboratories and research institutes.
- Wood preservative manufacturers, formulators and R&D teams.
- Certification bodies, regulators and specifiers in the timber protection sector.
Related standards
- EN 46-2 (Part 2): ovicidal action after egg‑laying.
- EN 47: determination of toxic values for Hylotrupes bajulus (referenced in introduction).
- EN 73 / EN 84: accelerated ageing procedures (evaporative / leaching) prior to biological testing.
- EN ISO 3696: water quality for analytical laboratory use.
Keywords: EN 46-1:2016, wood preservatives, Hylotrupes bajulus, surface treatment, laboratory method, preventive action, larvicidal testing, wood protection.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1: Anwendung durch Oberflächenverfahren (Laboratoriumsverfahren)Produits de préservation des bois - Détermination de l'action préventice contre les larves récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 1 : Application par traitement de surfave (Méthode de laboratoire)Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method)71.100.50Wood-protecting chemicalsICS:Ta slovenski standard je istoveten z:EN 46-1:2016SIST EN 46-1:2016en,fr,de01-september-2016SIST EN 46-1:2016SLOVENSKI
STANDARDSIST EN 46-1:20101DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 46-1
June
t r s x ICS
y sä s r rä w r Supersedes EN
v xæ sã t r r {English Version
Wood preservatives æ Determination of the preventive action against recently hatched larvae of Hylotrupes Produits de préservation du bois æ Détermination de l 5action préventive contre les larves récemment écloses
Holzschutzmittel æ Bestimmung der vorbeugenden Wirkung gegenüber frisch geschlüpften Larven von durch Oberflächenverfahren This European Standard was approved by CEN on
w January
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
t r s x CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
v xæ sã t r s x ESIST EN 46-1:2016
Example of a test report . 17 Annex B (informative)
Technique for culturing Hylotrupes bajulus (Linnaeus) . 19 B.1 General . 19 B.2 Obtaining parent beetles . 19 B.3 Mating . 19 B.4 Egg-Iaying . 19 SIST EN 46-1:2016
Differentiation of heartwood and sapwood in Pinus species . 22 C.1 Principle . 22 C.2 Reagents . 22 C.3 Apparatus . 22 C.4 Procedure . 22 Annex D (informative)
Environmental, health and safety precautions within chemical/biological laboratory . 23 Annex E (normative)
Slow acting and deferred effect formulations – Extending the test duration . 24 Bibliography . 26
introduction of new harmonized specifications for wood quality; b)
option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is water of drinking quality; c)
determination of validity of the test by using an X-ray apparatus added in 8.4.2. The standard EN 46 is composed of two parts: — EN 46-1, Wood preservatives
— EN 46-2, Wood preservatives
hatched
EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidial effect of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. SIST EN 46-1:2016
3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled [SOURCE: EN 1001-2:2005, 4.71] 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 1001–2:2005, 4.83. SIST EN 46-1:2016
1) In southern European countries the pine species most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this document has been demonstrated in all aspects (development of larvae, resistance to impregnation, etc.). SIST EN 46-1:2016
2) For special tests, test specimens may be obtained according to a given series. As a result, it may be preferable to take test specimens from pretreated strips. Where pretreated strips are used details should be included in the test report. SIST EN 46-1:2016
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Frequently Asked Questions
SIST EN 46-1:2016 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method)". This standard covers: This European Standard specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: - water-insoluble chemicals which are being studied as active insecticides; - organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; - organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and - water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.
This European Standard specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: - water-insoluble chemicals which are being studied as active insecticides; - organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; - organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and - water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.
SIST EN 46-1:2016 is classified under the following ICS (International Classification for Standards) categories: 71.100.50 - Wood-protecting chemicals. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN 46-1:2016 has the following relationships with other standards: It is inter standard links to SIST EN 46-1:2010, oSIST prEN 46-1:2025. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
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