kSIST FprEN ISO 10253:2024

SLOVENSKI STANDARD
oSIST prEN ISO 10253:2023
01-april-2023
Kakovost vode - Preskus zaviranja rasti morskih alg s Skeletonema sp. in
Phaeodactylum tricornutum (ISO/DIS 10253:2023)
Water quality - Marine algal growth inhibition test with Skeletonema sp. and
Phaeodactylum tricornutum (ISO/DIS 10253:2023)
Wasserbeschaffenheit - Wachstumshemmtest mit marinen Algen Skeletonema sp. und
Phaeodactylum tricornutum (ISO/DIS 10253:2023)
Qualité de l'eau - Essai d'inhibition de la croissance des algues marines avec
Skeletonema sp. et Phaeodactylum tricornutum (ISO/DIS 10253:2023)
Ta slovenski standard je istoveten z: prEN ISO 10253
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
oSIST prEN ISO 10253:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN ISO 10253:2023
oSIST prEN ISO 10253:2023
DRAFT INTERNATIONAL STANDARD
ISO/DIS 10253
ISO/TC 147/SC 5 Secretariat: DIN
Voting begins on: Voting terminates on:
2023-02-17 2023-05-12
Water quality — Marine algal growth inhibition test with
Skeletonema sp. and Phaeodactylum tricornutum
Qualité de l'eau — Essai d'inhibition de la croissance des algues marines avec Skeletonema sp. et
Phaeodactylum tricornutum
ICS: 13.060.70
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 10253:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

oSIST prEN ISO 10253:2023
ISO/DIS 10253:2023(E)
DRAFT INTERNATIONAL STANDARD
ISO/DIS 10253
ISO/TC 147/SC 5 Secretariat: DIN
Voting begins on: Voting terminates on:

Water quality — Marine algal growth inhibition test with
Skeletonema sp. and Phaeodactylum tricornutum
Qualité de l'eau — Essai d'inhibition de la croissance des algues marines avec Skeletonema sp. et
Phaeodactylum tricornutum
ICS: 13.060.70
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on STANDARD UNTIL PUBLISHED AS SUCH.
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
IN ADDITION TO THEIR EVALUATION AS
or ISO’s member body in the country of the requester. BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
ISO copyright office
USER PURPOSES, DRAFT INTERNATIONAL
CP 401 • Ch. de Blandonnet 8
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
CH-1214 Vernier, Geneva
POTENTIAL TO BECOME STANDARDS TO
Phone: +41 22 749 01 11
WHICH REFERENCE MAY BE MADE IN
Reference number
Email: copyright@iso.org
NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 10253:2022(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
Published in Switzerland
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2022

oSIST prEN ISO 10253:2023
ISO/DIS 10253:2022(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Materials . 3
5.1 Test organisms . 3
5.2 Reagents . 4
5.2.1 Water . 4
5.2.2 Synthetic Sea water . 4
5.2.3 Nutrients . 4
6 Apparatus . 5
7 Procedure .6
7.1 Preparation of growth medium. 6
7.2 Preparation of pre-culture and inoculum . 6
7.3 Choice of test concentrations . 6
7.4 Preparation of test substance stock solutions . 7
7.5 Preparation of test and control batches . 7
7.6 Incubation . 7
7.7 Measurements . 8
8 Validity criteria . 8
9 Interpretation of data . 8
9.1 Plotting growth curves . 8
9.2 Calculation of percentage inhibition . 9
9.3 Determination of EC(r) . 9
x
10 Expression of results . 9
11 Interpretation of results . .10
12 Test report .10
Annex A (informative) Preparation of dilution series of mixtures in sea water(effluents or
elutriates) .11
Annex B (informative) Test procedure starting from stored algal inocula, and with direct
measurement of algal growth in spectrophotometric cells .12
Annex C (informative) Performance data .18
Annex D (informative) Marine algal growth inhibition test with Phaeodactylum tricornutum
applied in 24-well-microwell plates .19
Bibliography .27
iii
oSIST prEN ISO 10253:2023
ISO/DIS 10253:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment,
as well as information about ISO's adherence to the World Trade Organization (WTO) principles in the
Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.
The committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 5, Biological
methods.
This fourth edition cancels and replaces the second edition (ISO 10253:2016), which has been
technically revised.
The main changes compared to the previous edition are as follows:
— in 5.2 Table 2, substitution of K PO with K HPO in stock solution 3;
3 4 2 4
— Annex D has been added to describe the marine algal growth inhibition test with Phaeodactylum
tricornutum applied in 24-well-microwell plates.
iv
oSIST prEN ISO 10253:2023
DRAFT INTERNATIONAL STANDARD ISO/DIS 10253:2022(E)
Water quality — Marine algal growth inhibition test with
Skeletonema sp. and Phaeodactylum tricornutum
WARNING — Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document be
carried out by suitably trained staff.
1 Scope
This document specifies a method for the determination of the inhibition of growth of the unicellular
marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in
sea water or by environmental water samples (effluents, elutriates, etc.).
The method can be used for testing substances that are readily soluble in water and are not significantly
degraded or eliminated in any other way from t
...