Soil quality - Sampling of soil invertebrates - Part 5: Sampling and extraction of soil macro-invertebrates (ISO 23611-5:2024)

ISO 23611-5:2011 specifies a method for sampling, extracting and preserving macro-invertebrates from soils, including the litter zone. The proposed method is a prerequisite for using these animals as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms). The main premise of this method is rapid assessment (completing the sampling of a plot in one or two days with only basic equipment and a small number of field assistants), in order to be able to address all the taxonomic groups of soil macro-invertebrates at the same time and in the same place. The Tropical Soil Biology and Fertility (TSBF) method has evolved and some modifications have been introduced in order to use it in temperate regions.
The sampling and extraction methods in ISO 23611-5:2011 are applicable to almost all types of soils, with the exception of soils in extreme climatic conditions (hard, frozen or flooded soils) and matrices other than soil, e.g. tree trunks, plants or lichens.
Sampling design is specified in ISO 23611-6.

Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 5: Probenahme und Extraktion von Makroinvertebraten (Großwirbellosen) im Boden (ISO 23611-5:2024)

Dieses Dokument legt ein Verfahren zur Probenahme, Extraktion und Konservierung von Makroinvertebraten aus Böden, einschließlich der Streuzone, fest.
Die Probenahme- und Extraktionsverfahren dieses Dokuments sind auf nahezu alle Bodenarten anwendbar mit Ausnahme von Böden aus extremen klimatischen Bedingungen (harte, gefrorene oder überflutete Böden) sowie anderen Matrices als Boden, z. B. Baumstämme, Pflanzen oder Flechten.

Qualité du sol - Prélèvement des invertébrés du sol - Partie 5: Prélèvement et extraction des macro-invertébrés du sol (ISO 23611-5:2024)

L'ISO 23611-5:2011 spécifie une méthode pour le prélèvement, l'extraction et la conservation des macro-invertébrés du sol, y compris la zone de litière. La méthode proposée est un prérequis à l'utilisation de ces animaux en tant que bio-indicateurs (par exemple pour évaluer la qualité d'un sol en tant qu'habitat pour des organismes). Les principes majeurs de cette méthode consistent à avoir une évaluation rapide (réaliser l'échantillonnage d'une zone en un ou deux jours avec un équipement de base et avec un nombre réduit d'assistants de terrain), pour être capable de traiter tous les groupes taxinomiques de macro‑invertébrés du sol en même temps et au même endroit. La méthode TSBF (biologie et fertilité des sols tropicaux) a évolué et quelques modifications ont été introduites afin de l'utiliser dans des régions tempérées.
Les méthodes de prélèvement et d'extraction de l'ISO 23611-5:2011 sont applicables à la quasi‑totalité des sols. Les sols présents sous des conditions climatiques extrêmes (sols durs, gelés ou inondés) et les matrices autres que le sol, par exemple des troncs d'arbres, des plantes ou des lichens, peuvent constituer des exceptions.
Le plan d'échantillonnage est spécifié dans l'ISO 23611-6.

Kakovost tal - Vzorčenje nevretenčarjev v tleh - 5. del: Vzorčenje in ekstrakcija velikih nevretenčarjev v tleh (ISO 23611-5:2024)

General Information

Status
Published
Public Enquiry End Date
02-Aug-2023
Publication Date
27-Jan-2025
Technical Committee
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
10-Jan-2025
Due Date
17-Mar-2025
Completion Date
28-Jan-2025

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SLOVENSKI STANDARD
01-marec-2025
Nadomešča:
SIST EN ISO 23611-5:2013
Kakovost tal - Vzorčenje nevretenčarjev v tleh - 5. del: Vzorčenje in ekstrakcija
velikih nevretenčarjev v tleh (ISO 23611-5:2024)
Soil quality - Sampling of soil invertebrates - Part 5: Sampling and extraction of soil
macro-invertebrates (ISO 23611-5:2024)
Bodenbeschaffenheit - Probenahme von Wirbellosen im Boden - Teil 5: Probenahme
und Extraktion von Makroinvertebraten (Großwirbellosen) im Boden (ISO 23611-5:2024)
Qualité du sol - Prélèvement des invertébrés du sol - Partie 5: Prélèvement et extraction
des macro-invertébrés du sol (ISO 23611-5:2024)
Ta slovenski standard je istoveten z: EN ISO 23611-5:2024
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 23611-5
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2024
EUROPÄISCHE NORM
ICS 13.080.30; 13.080.05 Supersedes EN ISO 23611-5:2013
English Version
Soil quality - Sampling of soil invertebrates - Part 5:
Sampling and extraction of soil macro-invertebrates (ISO
23611-5:2024)
Qualité du sol - Prélèvement des invertébrés du sol - Bodenbeschaffenheit - Probenahme von Wirbellosen
Partie 5: Prélèvement et extraction des macro- im Boden - Teil 5: Probenahme und Extraktion von
invertébrés du sol (ISO 23611-5:2024) Makroinvertebraten (Großwirbellosen) im Boden (ISO
23611-5:2024)
This European Standard was approved by CEN on 26 August 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23611-5:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 23611-5:2024) has been prepared by Technical Committee ISO/TC 190 "Soil
quality" in collaboration with Technical Committee CEN/TC 444 “Environmental characterization of
solid matrices” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2025, and conflicting national standards
shall be withdrawn at the latest by February 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 23611-5:2013.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 23611-5:2024 has been approved by CEN as EN ISO 23611-5:2024 without any
modification.
International
Standard
ISO 23611-5
Second edition
Soil quality — Sampling of soil
2024-08
invertebrates —
Part 5:
Sampling and extraction of soil
macro-invertebrates
Qualité du sol — Prélèvement des invertébrés du sol —
Partie 5: Prélèvement et extraction des macro-invertébrés du sol
Reference number
ISO 23611-5:2024(en) © ISO 2024

ISO 23611-5:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 23611-5:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 2
7 Field procedure . 3
7.1 General .3
7.2 Collecting macro-invertebrates from the litter zone .3
7.3 Collecting macro-invertebrates from soil .3
7.3.1 General .3
7.3.2 Temperate regions .3
7.3.3 Tropical regions.4
8 Laboratory procedure . 4
8.1 Treatment of collected samples .4
8.2 Preservation of specimens .5
8.3 Biomass determination .5
9 A ssessment of results . 6
10 Test report . 6
Annex A (informative) Background information . 7
Annex B (informative) Sampling soil macro-fauna using pitfall traps . 8
[68]
Annex C (informative) Monitoring example with pitfall traps . 9
Bibliography . 14

iii
ISO 23611-5:2024(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
characterization, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 444, Environmental characterization of solid matrices, in accordance with the Agreement
on technical cooperation between ISO and CEN (Vienna Agreement).
This second edition cancels and replaces the first edition (ISO 23611-5:2011), which has been technically
revised.
The main changes are as follows:
— Two informative Annexes were added at the end of the document. Annex B describes the procedures to
be adopted when sampling macro-fauna using pitfall traps and Annex C presents a monitoring example
with pitfall traps.
— The
...

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