SIST EN 17707:2025

SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17707:2023
Rastlinski biostimulanti - Določanje kvasovk in plesni
Plant biostimulants - Determination of the yeast and mould content
Pflanzen-Biostimulanzien - Bestimmung des Gehalts an Hefen und Schimmelpilzen
Biostimulants des végétaux - Détermination de la teneur en levures et en moisissures
Ta slovenski standard je istoveten z: EN 17707:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 17707
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17707:2022
English Version
Plant biostimulants - Determination of the yeast and
mould content
Biostimulants des végétaux - Détermination de la Pflanzen-Biostimulanzien - Bestimmung des Gehalts an
teneur en levures et en moisissures Hefen und Schimmelpilzen
This European Standard was approved by CEN on 26 August 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17707:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principles . 7
4.1 General . 7
4.2 Plate count method . 7
5 Diluent and culture media . 8
5.1 General . 8
5.2 Diluent . 8
5.3 Culture media . 8
6 Apparatus and glassware . 8
7 Handling of plant biostimulants and sampling . 8
8 Procedure. 8
8.1 General . 8
8.2 Test portion and initial suspension . 9
8.2.1 General . 9
8.3 Serial dilutions . 9
8.4 Plate count methods . 9
8.4.1 Pour plate method . 9
8.4.2 Surface spread method . 9
8.4.3 Incubation .10
9 Counting of colonies .10
10 Expression of results .10
10.1 Method of calculation .10
10.2 Interpretation .11
11 Test report .12
Annex A (normative) Composition and preparation of culture media and reagents .13
A.1 General .13
A.2 Phosphate buffered saline (PBS) .13
A.3 Sabouraud dextrose chloramphenicol agar medium (SDCA) .14
A.4 Potato dextrose agar (PDA) medium with antibiotics .14
A.5 Glucose-peptone (GP) agar medium with antibiotics .15
A.6 Malt extract agar (MEA) medium with antibiotics .15
Annex B (informative) Interlaboratory study .16
B.1 Materials used in the interlaboratory study .16
B.2 Results of the interlaboratory study . 17
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered . 19
Bibliography . 20
European foreword
This document (EN 17707:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17707:2022.
— the Introduction has been updated and Table 1 has been removed;
— normative references have been updated;
— in Clause 3, new terms and definitions have been added and others have been revised;
— Annexes A, B and C have been revised;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant
biostimulants intended for use in agriculture, by means of the colony count technique after aerobic
incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant
biostimulants, both in liquid and solid states. The method is applicable to microbial plant biostimulants
except those composed of fungi or yeasts.
If necessary, yeasts and moulds enumerated can be identified using suitable identification tests.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Plant Biostimulants, and where the following category Plant Biostimulants is the highest
% in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not
the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend
of fertilizing products is composed of components in equal quantity or in case the component EU
fertilising products used for the blend have identical formulations , the user decides which standard to
apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sampling
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
EN 17714:2024, Plant biostimulants — Determination of microorganisms’ concentration
EN 17724:2024, Plant biostimulants — Terminology
3 Terms and definitions
For the purposes of this document, the terms and definition given in EN 17724:2024 and the following
apply.
ISO and IEC maintain terminological databases for use in standardization at t
...