SIST EN ISO 25202:2026
(Main)Leather - Chemical analysis - Determination of glutaraldehyde content (ISO 25202:2026)
- Abstract
This document specifies a method for the determination of free and released glutaraldehyde in leathers, using liquid chromatography (LC).
- Status
- Published
- Public Enquiry End Date
- 27-Feb-2025
- Publication Date
- 27-Oct-2026
- Technical Committee
- IUSN - Leather
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 26-Oct-2026
- Due Date
- 31-Dec-2026
- Completion Date
- 28-Oct-2026
SIST EN ISO 25202:2026 is a standard for leather chemical analysis. It specifies an LC method for determining free and released glutaraldehyde in leather, for use in laboratory testing.
What does SIST EN ISO 25202:2026 specify?
SIST EN ISO 25202:2026 specifies a method for measuring glutaraldehyde content in leather by liquid chromatography (LC). The scope covers free glutaraldehyde and glutaraldehyde released during extraction.
The document is organized into clauses on scope, normative references, terms and definitions, principle, reagents, apparatus, specimen preparation, procedure, expression of results, and test report, followed by one informative annex.
| Annex | What it covers |
|---|---|
| Annex A | LC conditions, including example measurement conditions and a note on peak handling |
What are the key requirements of SIST EN ISO 25202:2026?
SIST EN ISO 25202:2026 is built around extraction, derivatization, LC separation, and calculation from a calibration graph. In practice, it gives a laboratory a full workflow from leather sampling to the reported result.
Sampling and specimen preparation - Clause 7 requires sampling in accordance with ISO 2418 and cutting leather in accordance with ISO 4044. If sampling from finished products is not possible, the sampling details must be included in the test report. This matters because the result is only as representative as the specimen taken.
Extraction of the leather sample - Clause 8.1.1 calls for about 2 g of leather pieces, extracted with 50 ml of detergent solution at 40 °C for 60 minutes in a shaken water bath. The leather/solution ratio must not be changed, and extraction and analysis should be carried out on the same working day. This keeps the test conditions comparable from sample to sample.
Reaction with DNPH - Clause 8.1.2 mixes the filtered extract with acetonitrile and 2,4-dinitrophenylhydrazine (DNPH), then allows at least 60 minutes for reaction before LC analysis. For samples with higher glutaraldehyde content, smaller aliquots are used. This step converts aldehydes into measurable derivatives.
Calibration approach - Clause 8.2.1 and 8.2.2 describe calibration solutions prepared from either glutaraldehyde solution or derivatized glutaraldehyde-DNPH solution. The example calibration range is 0.1 µg/ml to 2.0 µg/ml, corresponding under the stated conditions to 5 mg/kg to 100 mg/kg in leather. This gives the laboratory the range over which to build the calibration graph.
LC measurement - Clause 8.2.3 requires analysis by LC with UV detection, and Annex A gives example conditions for the LC system. The annex includes a reversed-phase C18 column, a 30 °C column temperature, 10 µl injection volume, and detection at (355 ± 5) nm. This is the practical set-up used to separate and measure the derivative.
Evaluation of the chromatogram - Clause 4 states that glutaraldehyde is separated and quantified as a derivative from other aldehydes and ketones. The note in Annex A says that, with a C18 reversed-phase column, glutaraldehyde-DNPH gives two peaks and the calibration area is the sum of both peaks. This matters when integrating chromatograms so the reported value reflects the whole derivative signal.
Reporting the result - Clause 9 requires the glutaraldehyde content to be expressed to the nearest 0.1 mg/kg. The result is calculated from the calibration graph and the sample mass, using the formula given in Clause 9.2. This gives a single result that can be compared with internal specifications or customer requirements.
Who uses SIST EN ISO 25202:2026?
SIST EN ISO 25202:2026 is used by leather testing laboratories, quality control teams, manufacturers, and buyers who need to measure glutaraldehyde in leather. It is relevant for routine chemical analysis of leather materials and for checking samples from finished products such as shoes or garments.
Which standards are used with SIST EN ISO 25202:2026?
SIST EN ISO 25202:2026 cites three normative references that support the method:
- ISO 2418 - gives the position and preparation of leather specimens for testing.
- ISO 3696 - specifies the water quality required for analytical laboratory use.
- ISO 4044 - covers preparation of chemical test samples from leather.
These referenced standards help ensure that sampling, water quality, and sample preparation are handled consistently before the LC measurement begins.
What does the SIST EN ISO 25202:2026 document contain?
The document contains the method principle, reagent list, apparatus list, specimen preparation instructions, step-by-step extraction and derivatization procedures, calibration instructions, and the calculation and reporting approach. It also includes an informative annex with example LC conditions and a bibliographic reference to ISO 17226-1.
Clause 8 gives the detailed laboratory procedure, including extraction, DNPH reaction, filtration, and LC analysis. Clause 9 gives the calculation path from calibration graph to mg/kg result, and Clause 10 lists the items that must appear in the test report.
Annex A provides the measurement conditions used as an example for the LC system. It is the part to consult when setting up the chromatographic method in the laboratory.
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Frequently Asked Questions
SIST EN ISO 25202:2026 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Leather - Chemical analysis - Determination of glutaraldehyde content (ISO 25202:2026)". This standard covers: This document specifies a method for the determination of free and released glutaraldehyde in leathers, using liquid chromatography (LC).
This document specifies a method for the determination of free and released glutaraldehyde in leathers, using liquid chromatography (LC).
SIST EN ISO 25202:2026 is classified under the following ICS (International Classification for Standards) categories: 59.140.30 - Leather and furs; 71.040.40 - Chemical analysis. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 25202:2026 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2026
Usnje - Kemijska analiza - Ugotavljanje vsebnosti glutaraldehida (ISO 25202:2026)
Leather - Chemical analysis - Determination of glutaraldehyde content (ISO 25202:2026)
Leder - Chemische Analyse - Bestimmung des Glutaraldehydgehalts (ISO 25202:2026)
Cuir - Analyse chimique - Détermination de la teneur en glutaraldéhyde (ISO
25202:2026)
Ta slovenski standard je istoveten z: EN ISO 25202:2026
ICS:
59.140.30 Usnje in krzno Leather and furs
71.040.40 Kemijska analiza Chemical analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 25202
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2026
EUROPÄISCHE NORM
ICS 59.140.30
English Version
Leather - Chemical analysis - Determination of
glutaraldehyde content (ISO 25202:2026)
Cuir - Analyse chimique - Détermination de la teneur Leder - Chemische Analyse - Bestimmung des
en glutaraldéhyde (ISO 25202:2026) Glutaraldehydgehalts (ISO 25202:2026)
This European Standard was approved by CEN on 8 October 2026.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2026 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 25202:2026 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 25202:2026) has been prepared by Technical Committee ISO/IULTCS
"International Union of Leather Technologists and Chemists Societies" in collaboration with Technical
Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2027, and conflicting national standards shall be
withdrawn at the latest by April 2027.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 25202:2026 has been approved by CEN as EN ISO 25202:2026 without any modification.
International
Standard
ISO
IULTCS
IUC 40
First edition
Leather — Chemical analysis —
2026-10
Determination of glutaraldehyde
content
Cuir — Analyse chimique — Détermination de la teneur en
glutaraldéhyde
Reference numbers
ISO 25202:2026(en)
IULTCS/IUC 40:2026(en) © ISO 2026
ISO 25202:2026(en)
IULTCS/IUC 40:2026(en)
© ISO 2026
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Published in Switzerland
ii
ISO 25202:2026(en)
IULTCS/IUC 40:2026(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Preparation of test specimens . 3
8 Procedure . 3
8.1 Sample processing .3
8.1.1 Extraction .3
8.1.2 Reaction with DNPH .3
8.2 Determination by LC .3
9 Expression of results . 4
9.1 General .4
9.2 Calculation of the glutaraldehyde content .4
9.3 Precision of the test method .4
10 Test report . 4
Annex A (informative) Liquid chromatography conditions. 5
Annex B (informative) Precision: reliability of the method . 6
iii
ISO 25202:2026(en)
IULTCS/IUC 40:2026(en)
Foreword
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