Fertilizers - Determination of 3-methylpyrazole (MP) using high-performance liquid chromatography (HPLC)

This European Standard specifies a method for the determination of the 3-methylpyrazole (MP) content in fertilizers, in particular in urea and materials containing urea using high-performance liquid chromatography (HPLC).

Düngemittel - Bestimmung von 3-Methylpyrazol (MP) mit Hochleistungs-Flüssigchromatographie (HPLC)

Diese Europäische Norm legt ein Verfahren zur Bestimmung des Gehalts von 3 Methylpyrazol (MP) in Dünge-mitteln, insbesondere in Harnstoff und harnstoffhaltigen Materialien, unter Anwendung der Hochleistungs-Flüssig¬chromatographie (HPLC) fest.

Engrais - Dosage du 3-méthylpyrazole (MP) par chromatographie liquide haute performance (CLHP)

La présente norme européenne décrit une méthode de détermination de la teneur en 3-méthylpyrazole (MP) dans les engrais, en particulier dans l’urée et dans les matières contenant de l’urée, par chromatographie liquide à haute performance (HPLC).

Gnojila - Določevanje 3-metilpirazola (MP) s tekočinsko kromatografijo visoke ločljivosti (HPLC)

Ta evropski standard opredeljuje metodo za določevanje vsebnosti 3-metilpirazola (MP) v gnojilih, zlasti v sečnini in materialih, ki vsebujejo sečnino s tekočinsko kromatografijo visoke ločljivosti.

General Information

Status
Published
Public Enquiry End Date
19-Jul-2009
Publication Date
08-Nov-2010
ICS
65.080 - Fertilizers
Technical Committee
KAT - Soil quality
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
27-Sep-2010
Due Date
02-Dec-2010
Completion Date
09-Nov-2010
Directive
2003/2003 - Regulation (EC) N° 2003/2003 of the European Parlament and of the council of 13 October 2003 relating to fertilisers
Mandate
M/335 - Standardization Mandate assigned to the CEN concerning the modernisation of the methods of anylysis of Fertilizers
Ref Project
EN 15905:2010 - Fertilizers - Determination of 3-methylpyrazole (MP) using high-performance liquid chromatography (HPLC)

Overview

EN 15905:2010 (CEN) specifies a validated analytical method for the determination of 3‑methylpyrazole (MP) in fertilizers - in particular urea and urea-containing materials - using high‑performance liquid chromatography (HPLC). The standard describes the principle, required reagents and apparatus, sample preparation, HPLC operating conditions, calibration, calculation and expression of results, precision data from an inter‑laboratory trial, and reporting requirements.

Keywords: EN 15905:2010, 3‑methylpyrazole, MP, HPLC, fertilizers, urea analysis, CEN.

Key Topics and Requirements

  • Scope and principle
    • Quantitative determination of MP after dissolution/extraction in water and analysis by reversed‑phase HPLC with UV detection.
  • Reagents
    • HPLC‑grade acetonitrile, de‑ionized water (EN ISO 3696 grade 3), and a 3‑methylpyrazole standard.
  • Apparatus
    • Analytical balance (±0.1 mg), ultrasonic bath, filtration (0.45 µm membrane), volumetric flasks, isocratic HPLC system, UV detector, reversed‑phase column (e.g. C18).
  • Sample preparation
    • Dissolve or extract sample in water (follow EN 1482‑2 for sample prep). Use ultrasonic dissolution and membrane filtration; consider dilution for solid fertilizers.
  • HPLC conditions (typical)
    • Mobile phase: water/acetonitrile 80:20 (v/v), flow 0.8–1.2 mL/min, injection 20 µL, detection at 214 nm, column temperature ~room temperature. Degas mobile phase.
  • Calibration and calculation
    • Prepare calibration solutions from a primary MP standard and build a calibration curve (correlation coefficient ≥ 0.99). Calculate mass fraction using peak height or area and specified formula; report mean of two injections to 0.001%.
  • Precision
    • Inter‑laboratory trial (2008, 9 labs) reported mean MP values ~0.0304% and 0.0345%. Repeatability (r) and reproducibility (R) limits are provided (e.g., R ≈ 0.0039% for tested matrices).

Applications and Users

  • Who uses it: fertilizer manufacturers, quality control and analytical laboratories, certification bodies, regulatory agencies, and research institutions performing fertilizer composition analysis.
  • Practical uses:
    • Routine QC of urea‑based fertilizers to quantify MP additive levels.
    • Compliance testing against regulatory requirements and product specifications.
    • Method validation and inter‑laboratory comparisons for MP in fertilizer matrices.

Related Standards

  • EN 1482‑1 / EN 1482‑2 - Sampling and sample preparation for fertilizers
  • EN 12944‑1 / EN 12944‑2 - Vocabulary for fertilizers
  • EN ISO 3696 - Water for analytical laboratory use
  • ISO 5725 series - Accuracy (trueness and precision) of measurement methods
  • Regulation (EC) No 2003/2003 - Fertilisers (background regulatory context)

This concise summary of EN 15905:2010 helps laboratory managers and analysts implement a standardized HPLC method for reliable determination of 3‑methylpyrazole in urea and related fertilizer products.

Frequently Asked Questions

SIST EN 15905:2010 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Fertilizers - Determination of 3-methylpyrazole (MP) using high-performance liquid chromatography (HPLC)". This standard covers: This European Standard specifies a method for the determination of the 3-methylpyrazole (MP) content in fertilizers, in particular in urea and materials containing urea using high-performance liquid chromatography (HPLC).

This European Standard specifies a method for the determination of the 3-methylpyrazole (MP) content in fertilizers, in particular in urea and materials containing urea using high-performance liquid chromatography (HPLC).

SIST EN 15905:2010 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers. The ICS classification helps identify the subject area and facilitates finding related standards.

SIST EN 15905:2010 is associated with the following European legislation: EU Directives/Regulations: 2003/2003; Standardization Mandates: M/335. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.

SIST EN 15905:2010 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.

Standards Content (Sample)


2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von 3-Methylpyrazol (MP) mit Hochleistungs-Flüssigchromatographie (HPLC)Engrais - Dosage du 3-méthylpyrazole (MP) par chromatographie liquide haute performance (CLHP)Fertilizers - Determination of 3-methylpyrazole (MP) using high-performance liquid chromatography (HPLC)65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 15905:2010SIST EN 15905:2010en,fr,de01-december-2010SIST EN 15905:2010SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15905
June 2010 ICS 65.080 English Version
Fertilizers - Determination of 3-methylpyrazole (MP) using high-performance liquid chromatography (HPLC)
Engrais - Dosage du 3-méthylpyrazole (MP) par chromatographie liquide à haute performance (HPLC)
Düngemittel - Bestimmung von 3-Methylpyrazol (MP) mit Hochleistungs-Flüssigchromatographie (HPLC) This European Standard was approved by CEN on 29 April 2010.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
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B-1000 Brussels © 2010 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15905:2010: ESIST EN 15905:2010

Results of the inter-laboratory test .8Bibliography .9 SIST EN 15905:2010
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