EN 14105:2003

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.HWRGDErzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester (FAME) - Bestimmung des Gehaltes an freiem und Gesamtglycerin und Mono-, Di- und Triglyceriden (Referenzmethode)Produits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) - Détermination de la teneur en glycérols libre et total et en mono-, di- et triglycérides - Méthode de référenceFat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of free and total glycerol and mono-, di-, triglyceride contents (Reference method)67.200.10Animal and vegetable fats and oilsICS:Ta slovenski standard je istoveten z:EN 14105:2003SIST EN 14105:2003en01-november-2003SIST EN 14105:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14105April 2003ICS 67.200.10English versionFat and oil derivatives - Fatty Acid Methyl Esters (FAME) -Determination of free and total glycerol and mono-, di-,triglyceride contents (Reference method)Produits dérivés des corps gras - Esters méthyliquesd'acides gras (EMAG) - Détermination de la teneur englycérols libre et total et en mono-, di- et triglycérides -Méthode de référenceErzeugnisse aus pflanzlichen und tierischen Fetten undÖlen - Fettsäure-Methylester (FAME) - Bestimmung desGehaltes an freiem und Gesamtglycerin und Mono-, Di- undTriglyceriden (Referenzmethode)This European Standard was approved by CEN on 2 January 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14105:2003 ESIST EN 14105:2003

shall be given the status of a national standard, either by publication of an identical text orby endorsement, at the latest by October 2003, and conflicting national standards shall be withdrawn at the latestby October 2003.This document has been prepared under Mandate M/245 on Fatty Acid Methylester (FAME) given to CEN by theEuropean Commission and the European Free Trade Association.Annexes A to D are informative.According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal,Slovakia, Spain, Sweden, Switzerland and the United Kingdom.SIST EN 14105:2003

(to the nearest0,1 mg) in a 10 ml volumetric flask (4.5) and make up to the mark with pyridine (3.2).3.11Monoglycerides1), commercial mixture.Made up of monopalmitoylglycerol (monopalmitin), monostearoylglycerol (monostearin) and of monooleoylglycerol(monoolein), present in quantities having an identical mass.Prepare a stock solution of this mixture by weighing approximately 100 mg in a 10 ml volumetric flask (4.5) andmake up to the mark with pyridine (3.2).3.12Calibration solutionsPrepare daily four calibration solutions by transferring into a series of vials (4.6) the volumes of stock solutions ofreference substances (3.9 and 3.10) and of internal standards (3.7 and 3.8) given in Table 1, using microsyringes(4.8 and 4.9). The choice of the appropriate syringe shall be done according to Table 1. Do not use syringe atmaximum capacity, but dispense the half volume twice (i. e.: in case of 100 ml dosing using a 100 ml syringe, load50 ml twice). Be sure that needle and body of syringe are free from air bubbles, and measure volumes only bydifference (i. e.: when dispensing 80 ml, fill syringe up to 100 ml and supply solution up to the 20 ml mark).NOTEThe silylated standard solutions are only stable one day.Table 1 — Preparation of calibration solutionsCalibration solution1234Syringe, mlml of glycerol solution104070100100ml of monoolein solution50120190250500ml of diolein solution104070100100ml of triolein solution10306080100ml of internal std sol. No. 180808080100ml of internal std sol. No. 21001001001005003.13Carrier gas, hydrogen or helium.3.14Auxiliary gases:¾ air;¾ hydrogen.
1)Products available commercially from SIGMA, reference 178-8 (for example). This information is given for the convenience of users of thisEuropean Standard and does not constitute an endorsement by CEN of these products.SIST EN 14105:2003
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