EN ISO 6529:2001

SLOVENSKI STANDARD
01-junij-2002
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SIST EN 369:1996
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Protective clothing - Protection against chemicals - Determination of resistance of
protective clothing materials to permeation by liquids and gases (ISO 6529:2001)
Schutzkleidung - Schutz gegen Chemikalien - Bestimmung des Widerstands von
Schutzkleidungsmaterialien gegen die Permeation von Flüssigkeiten und Gasen (ISO
6529:2001)
Vetements de protection - Protection contre les produits chimiques - Détermination de la
résistance des matériaux utilisés pour la confection des vetements de protection a la
perméation par des liquides et des gaz (ISO 6529:2001)
Ta slovenski standard je istoveten z: EN ISO 6529:2001
ICS:
13.340.10 Varovalna obleka Protective clothing
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 6529
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2001
ICS 13.340.10 Supersedes EN 369:1993
English version
Protective clothing - Protection against chemicals -
Determination of resistance of protective clothing materials to
permeation by liquids and gases (ISO 6529:2001)
Vêtements de protection - Protection contre les produits Schutzkleidung - Schutz gegen Chemikalien - Bestimmung
chimiques - Détermination de la résistance des matériaux des Widerstands von Schutzkleidungsmaterialien gegen
utilisés pour la confection des vêtements de protection à la die Permeation von Flüssigkeiten und Gasen (ISO
perméation par des liquides et des gaz (ISO 6529:2001) 6529:2001)
This European Standard was approved by CEN on 4 October 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6529:2001 E
worldwide for CEN national Members.

CORRECTED  2002-10-16
Foreword
This document (ISO 6529:2001) has been prepared by Technical Committee ISO/TC 94
"Personal safety - Protective clothing and equipment" in collaboration with Technical
Committee CEN/TC 162 "Protective clothing including hand and arm protection and
lifejackets", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by April 2002, and conflicting national
standards shall be withdrawn at the latest by April 2002.
This document supersedes EN 369:1993.
This document has been prepared under a mandate given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements
of EU Directive(s).
For relationship with EU Directive(s), see informative annex ZB, which is an integral part of
this document.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
Endorsement notice
The text of ISO 6529:2001 has been approved by CEN as EN ISO 6529:2001 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 139 1973 Textiles - Standard atmospheres EN 20139 1992
for conditioning and testing
ISO 2286-2 1998 Rubber- or plastics-coated fabrics EN ISO 2286-2 1998
- Determination of roll
characteristics - Part 2: Methods
for determination of total mass per
unit area, mass per unit area of
coating and mass per unit area of
substrate
ISO 2286-3 1998 Rubber- or plastics-coated fabrics EN ISO 2286-3 1998
- Determination of roll
characteristics - Part 3: Method for
determination of thickness
ISO 5084 1996 Textiles - Determination of EN ISO 5084 1996
thickness of textiles and textile
products
Annex ZB
(informative)
Clauses of this European Standard addressing essential requirements or other
provisions of EU Directives
By agreement between ISO and CEN, this CEN annex is included in the DIS and the FDIS
but will not appear in the published ISO standard.
This European Standard has been prepared under a mandate given to CEN by the European
Commission and supports essential requirements of EU Directive 89/686/EEC, Annex II.
Note Other requirements and other EU Directives may be applicable to the product(s)
falling within the scope of this standard.
Subclause 3.10.2 of this standard is likely to support requirements of EU Directive
89/686/EEC, Annex II.
Compliance with this subclause provides one means of conforming with the specific essential
requirements of the Directive concerned.
INTERNATIONAL ISO
STANDARD 6529
Second edition
2001-10-15
Protective clothing — Protection against
chemicals — Determination of resistance of
protective clothing materials to permeation
by liquids and gases
Vêtements de protection — Protection contre les produits chimiques —
Détermination de la résistance des matériaux utilisés pour la confection
des vêtements de protection à la perméation par des liquides et des gaz
Reference number
ISO 6529:2001(E)
©
ISO 2001
ISO 6529:2001(E)
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ii © ISO 2001 – All rights reserved

ISO 6529:2001(E)
Contents Page
Foreword.iv
Introduction.v
1 Scope .1
2 Normative references .1
3 Terms and definitions .2
4 Principle.5
5 Choice of analytical technique and collection medium.5
5.1 General.5
5.2 Gaseous collection medium.5
5.3 Liquid collection medium .6
5.4 Other collection medium.6
6 Apparatus .6
7 Sampling.11
7.1 Sampling procedure .11
7.2 Preparation of test specimens .12
7.3 Measurement of test specimen thickness and mass.12
8 Procedure .12
8.1 Calibration .12
8.2 Preparation of test apparatus.12
8.3 Method A — Liquid chemicals with continuous contact.14
8.4 Method B — Gaseous chemicals with continuous contact .14
8.5 Method C — Liquid or gaseous chemical with intermittent contact .16
8.6 Preparation of permeation plot .17
8.7 Determination of breakthrough detection time and normalized breakthrough detection time.17
8.8 Calculation of permeation rate and cumulative permeation .18
8.9 Visual assessment of test specimen .19
8.10 Repeat tests.19
9 Report .20
Annex A (informative) Recommended list of chemicals for comparing permeation resistance of
protective clothing materials.22
Annex B (informative) Precision information for test method.24
Annex C (informative) Sources of permeation test cells and permeation test cell parts .25
Annex D (informative) Suggested procedure for measuring the sensitivity of open-loop permeation-
test systems .26
Bibliography.29
ISO 6529:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 6529 was prepared by Technical Committee ISO/TC 94, Personal safety — Protective
clothing and equipment, Subcommittee SC 13, Protective clothing.
This second edition cancels and replaces the first edition (ISO 6529:1990), which has been technically revised.
Annexes A to D of this International Standard are for information only.
iv © ISO 2001 – All rights reserved

ISO 6529:2001(E)
Introduction
Workers involved in the production, use, transportation, and emergency response with liquid and gaseous
chemicals can be exposed to numerous compounds capable of causing harm upon contact with the human body.
The deleterious effects of these chemicals can range from acute trauma such as skin irritation and burn to chronic
degenerative disease, such as cancer. Since engineering controls may not eliminate all possible exposures,
attention is often placed on reducing the potential for direct skin contact through the use of protective clothing that
resists permeation, penetration and degradation.
These test methods are normally used to evaluate the barrier effectiveness of materials used for protective clothing
and specimens from finished items (see Note 1) of protective clothing against permeation of either liquid or
gaseous chemicals. Options are provided for conducting this testing under both conditions of continuous or
intermittent contact with the chemicals.
These test methods provide various options for reporting test results in terms of breakthrough time, permeation rate
and cumulative permeation to allow a comparison of protective clothing material permeation resistance. These
parameters are key measures of the effectiveness of a clothing material to act as a barrier to the test chemical.
Such information is used in the comparison of clothing materials during the process of selecting clothing for
protection from hazardous chemicals. Long breakthrough detection times and normalized breakthrough detection
times as well as low permeation rates are characteristic of the best barriers.
Resistance to penetration by liquid chemicals should be determined by using ISO 6530 while resistance to
penetration by liquid chemicals under pressure should be determined by using ISO 13994. These International
Standards are listed in the Bibliography.
It has been assumed in the drafting of this International Standard that the execution of its provisions is entrusted to
appropriately qualified and experienced people for whose guidance it has been prepared and that appropriate
precautions will be taken to avoid injury to health and contamination of the environment.
NOTE 1 Finished items of protective clothing include gloves, arm shields, aprons, suits, hoods, boots, etc. The phrase
“specimens from finished items” encompasses seamed and other discontinuous regions as well as the usual continuous regions
of protective clothing items.
NOTE 2 At present, no quantitative information exists about acceptable levels of dermal contact. Therefore, the data obtained
using this test method cannot be used to infer safe exposure levels.
INTERNATIONAL STANDARD ISO 6529:2001(E)
Protective clothing — Protection against chemicals —
Determination of resistance of protective clothing materials to
permeation by liquids and gases
1 Scope
This International Standard describes laboratory test methods that enable a determination of the resistance of
materials used in protective clothing to permeation by liquid or gaseous chemicals under the conditions of either
continuous or intermittent contact.
Method A (see 8.3) is applicable to the testing of liquid chemicals, either volatile or soluble in water, expected to be
in continuous contact with the protective clothing material.
Method B (see 8.4) is applicable to the testing of gaseous chemicals expected to be in continuous contact with the
protective clothing material.
Method C (see 8.5) is applicable to the testing of liquid chemicals, either volatile or soluble in water, expected to be
in intermittent contact with the protective clothing material.
These test methods are only suitable for the testing of air-impermeable protective clothing materials. They assess
the permeation resistance of the protective clothing material under laboratory conditions in terms of breakthrough
time, permeation rate, and cumulative permeation. These test methods also enable observations to be made of the
effects of the test liquid on the protective clothing material under test.
These test methods address only the performance of materials or certain material constructions (e.g. seams) used
in protective clothing. These test methods do not address the design, overall construction and components, or
interfaces of garments or other factors which may affect the overall protection offered by the protective clothing.
It is emphasized that these tests do not necessarily simulate conditions to which clothing materials are likely to be
exposed in practice. The use of test data should therefore be restricted to broad comparative assessment of such
material according to their permeation-resistance characteristics.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 139, Textiles — Standard atmospheres for conditioning and testing
ISO 2286-2, Rubber- or plastics-coated fabrics — Determination of roll characteristics — Part 2: Methods for
determination of total mass per unit area, mass per unit area of coating and mass per unit area of substrate
ISO 2286-3, Rubber- or plastics-coated fabrics — Determination of roll characteristics — Part 3: Method for
determination of thickness
ISO 6529:2001(E)
ISO 3801, Textiles — Woven fabrics — Determination of mass per unit length and mass per unit area
ISO 5084, Textiles — Determination of thickness of textiles and textile products
3 Terms and definitions
For the purposes of this International Standard, the following terms and definitions apply.
3.1
analytical technique
procedure whereby the concentration of a chemical in a collection medium is quantitatively determined
NOTE These procedures are often specific to individual chemical and collection-medium combinations.
EXAMPLES Applicable analytical techniques can include ultraviolet (UV) and infrared (IR) spectrophotometry, gas and
liquid chromatography, pH measurement, ion chromatography, conductimetry, colorimetry, atmospheric analytical detector
tubes and radionuclide tagging/detection counting.
3.2
breakthrough detection time
elapsed time measured from the start of the test to the sampling time that immediately precedes the sampling time
at which the test chemical is first detected
SeeFigure1.
NOTE The breakthrough detection time is dependent on the sensitivity of the method and the frequency of sampling (the
interval between sampling times).
3.3
closed-loop
refers to a testing mode in which the collection medium volume is fixed
NOTE The collection medium volume may change slightly from sampling without replacement of the sampled collection
medium.
3.4
collection medium
liquid or gas that does not affect the measured permeation and in which the test chemical is freely soluble or
adsorbed to a saturation concentration greater than 0,5 % by mass or by volume
3.5
contact time
in an intermittent contact test, the duration that the challenge-side chamber of the permeation cell contains test
chemical during each cycle
3.6
cumulative permeation mass
total amount of chemical that permeates during a specified time from the time the clothing material specimen is first
contacted with the test chemical
NOTE 1 Quantification of cumulative permeation enables the comparison of permeation behaviour under different intermittent
and continuous-contact conditions.
NOTE 2 The measurement of cumulative permeation may depend on the sensitivity of the permeation-test system.
3.7
cycle time
in an intermittent contact permeation test, the interval of time from the start of one contact period to the start of the
next contact period
2 © ISO 2001 – All rights reserved

ISO 6529:2001(E)
NOTE The breakthrough detection time for a method sensitivity of 0,05 �g/cm /min is 23 min but would be reported at
20 min, which corresponds to the last sampling time preceding the test. The normalized breakthrough detection time at a
normalization permeation rate of 0,1 �g/cm /min is 33 min, but similarly would be reported at 28 min, which corresponds to the
preceding sampling time. The steady-state permeation rate is approximately 0,15 �g/cm /min.
Figure 1 — Breakthrough detection time
3.8
degradation
deleterious change in one or more physical properties of a protective clothing material
3.9
minimum detectable mass permeated
smallest mass of test chemical that is detectable with the complete permeation-test system
NOTE This value is not necessarily the intrinsic limit of detection for the analytical instrument.
3.10
minimum detectable permeation rate
lowest rate of permeation that is measurable with the complete permeation-test system
NOTE This value is not necessarily the intrinsic limit of detection for the analytical instrument.
3.11
normalization permeation mass
permeation mass used for determining the normalized breakthrough detection time in a closed-loop permeation test
ISO 6529:2001(E)
2 2
NOTE This test method provides two choices of normalization permeation mass at 0,25 �g/cm or 2,5 �g/cm .
3.12
normalization permeation rate
permeation rate used for determining the normalized breakthrough detection time in an open-loop permeation test
2 2
NOTE This test method provides two choices of normalization permeation rates: 0,1 �g/cm /min or 1,0 �g/cm /min.
3.13
normalized breakthrough detection time
�open-loop system� time at which the permeation rate reaches the normalization permeation rate
SeeFigure1.
3.14
normalized breakthrough detection time
�closed-loop test� time at which the mass of chemical permeated reaches the normalization permeation mass
3.15
open-loop
testing mode in which fresh collection medium flows continuously through the collection chamber of the test cell
and is not reused or recycled
3.16
penetration
flow of a chemical through closures, porous materials, seams and holes or other imperfections in a protective
clothing material on a non-molecular level
3.17
permeation
process by which a chemical moves through a protective clothing material on a molecular level
NOTE Permeation involves
a) sorption of molecules of the chemical into the contacted (outside) surface of a material,
b) diffusion of the sorbed molecules in the material, and
c) desorption of the molecules from the opposite (inside) surface of the material into the collection medium.
3.18
permeation mass
quantity of test chemical that passes through the protective clothing material within a given time
3.19
permeation rate
quantity of test chemical that passes through the protective clothing material for a given exposed surface area per
unit time
3.20
protective clothing material
any material or combination of materials used in an item of clothing for the purpose of isolating parts of the body
from a potential hazard
3.21
purge time
�intermittent contact test� time immediately following the termination of the contact time when the test chemical is
removed from the challenge-side chamber and air or nitrogen is blown over the outside surface of the protective
clothing material
4 © ISO 2001 – All rights reserved

ISO 6529:2001(E)
3.22
steady-state permeation rate
constant rate of permeation that occurs after breakthrough when the chemical contact is continuous and all forces
affecting permeation have reached equilibrium
NOTE Steady-state permeation may not be achieved during the period for which permeation testing is conducted.
3.23
test chemical
liquid or gas that is used to challenge the protective clothing material specimen
NOTE The liquid or gas may be either one component (i.e. a neat liquid or gas) or have several components (i.e. a mixture).
4Principle
The protective clothing material specimen acts as a partition between one chamber of a permeation test cell, which
contains the test chemical, and another chamber, which contains the collection medium.
The test chemical may be either a liquid or a gas. The protective clothing material specimen may contact the test
chemical either continuously or intermittently depending on the choice of the method used.
The collection medium, which may be liquid or gas, is analysed quantitatively for its concentration of the chemical
and thereby the amount of that chemical that has permeated the partition as a function of time after its initial
contact with the material.
Several different test configurations may be used depending on the choice of the test chemical, collection medium
and conditions of the test.
By either graphical representation or appropriate calculations, or both, the breakthrough detection time, normalized
breakthrough detection time, permeation rate, and cumulative permeation of the test chemical are determined.
A group of chemicals, representing a range in chemical properties, which can be used to compare the permeation
resistance is given in annex A.
Interlaboratory data for this test method are provided in annex B.
5 Choice of analytical technique and collection medium
5.1 General
The combination of the analytical technique and the collection medium shall be selected to maximize sensitivity for
the detection of the test chemical and represent actual occupational conditions as closely as possible.
5.2 Gaseous collection medium
The gaseous collection medium shall be either dry air or a dry, non-flammable inert gas, or other gases which do
not interfere with the detection of the test chemical and are of sufficiently high purity as not to interfere with the
permeation process or analytical procedure.
EXAMPLES Nitrogen or helium.
NOTE This gas is used, under continuous flow conditions, for the collection of permeating molecules that are capable of
vaporizing from the test liquid under the conditions of the test in sufficient quantities for analysis.
ISO 6529:2001(E)
5.3 Liquid collection medium
The liquid collection medium shall be either water or another liquid which does not influence the resistance of the
protective clothing material to permeation.
NOTE This liquid is used for the collection of diffused molecules of low volatility that are soluble in the collecting medium
under the conditions of the test in sufficient quantities for analysis.
5.4 Other collection medium
Other collection medium such as solid sorbents may be used when suitable collection efficiencies are
demonstrated for the test chemical being used.
6 Apparatus
6.1 Thickness gage, suitable for measuring thickness to the nearest 0,02 mm, as specified in ISO 2286-3 or
ISO 5084, and used to determine the thickness of each protective clothing material specimen tested.
6.2 Analytical balance, capable of being read to the nearest 0,01 g.
6.3 Permeation test cell, consisting of a two-chambered cell for contacting the specimen with the test chemical
on the specimen's normal outside surface (clothing exterior) and with a collection medium on the specimen's
normal inside surface (clothing interior).
NOTE Permeation test cells may be designed for testing either liquid test chemicals or gaseous test chemicals. However,
alternative permeation test cells may also be used.
6.3.1 Permeation test cell for liquid test chemicals, capable of accommodating liquid chemicals, constructed
from two end-fitting sections of straight glass pipe, each nominally sized to either a 25 mm or 51 mm diameter (see
Figure 2).
Materials other than glass may be used for tests involving chemicals incompatible with glass (e.g. hydrofluoric
acid).
Permeation test cells made from glass shall be arranged as follows.
� One end of each glass section shall be sealed closed (for example, with a glass disk equivalent in quality to
that of the glass of the original sections).
� The opposite end of each glass section shall retain the “as-manufactured” flared end.
� Inlet and outlet ports with appropriate stopcock valves shall be added to each glass section as shown.
� When assembled, the two glass sections shall be joined horizontally by flanges and a gasket shall be used at
the joint. A second gasket may be used on the other side of the test specimens if necessary, to obtain a proper
seal.
EXAMPLE PTFE gasket material.
� In closed-loop tests where increased analytical sensitivity is required, a shorter length of glass pipe may be
used to contain the collection medium. This reduces the contained volume and increases the ratio of material
specimen area to the collection medium volume. In open-loop tests, lower collection medium flow rates will
increase the system sensitivity by lowering the minimum detectable permeation rate.
The challenge-side chamber may be modified to include an additional outlet port (with stopcock) positioned
downward, opposite the liquid chemical inlet port. Such a modification will facilitate the repeated addition and
removal of liquid test chemicals during intermittent testing.
6 © ISO 2001 – All rights reserved

ISO 6529:2001(E)
Key
1 Replaceable stirring rod 5 Fill level
2 Challenge chemical inlet 6 Challenge side for test chemical
3 Stop cock adapter 7 Sampling side for collection medium
4 Gasket 8 Aluminium flanges
Figure 2 — Permeation test cell for liquid test chemicals
The clothing material specimen is positioned between the flared ends of the two glass sections that compose the
test cell. When the specimen is in place, the test cell is divided into two chambers.
NOTE Sources for permeation test cell parts are provided in annex C.
6.3.2 Permeation test cell for gaseous test chemicals (see Figure 3), capable of accommodating gaseous
chemicals, identical to the liquid permeation test cell except that, with reference to Figure 2, two collection medium
sections are used. Thus, the gaseous chemical can be circulated from its reservoir through the challenge-side
chamber. Flow shall be such that the composition and concentration of the gas in the test chamber does not
change with time and the test gas in the chamber is well mixed.
A specimen is positioned between the flared ends of the two glass sections that compose the test cell as shown in
Figure 3. When the specimen is in place, the test cell is divided into two chambers.
ISO 6529:2001(E)
Key
1 Sampling side for collection medium 5 Protective clothing material specimen
2 Challenge side for test chemical 6 Gasket
3Valve 7 Aluminium flanges
4 Filling tube
Figure 3 — Permeation test cell configured for intermittent contact (top view)
used for gaseous test chemicals
6.3.3 Alternative permeation test cells, for conducting permeation testing, see for example Figure 4.
6.4 Equipment for open-loop permeation testing, consisting of the components listed in 6.4.1 to 6.4.6 and
shown inFigure5.
In open-loop testing, select and configure equipment to provide the desired test system sensitivity in terms of the
minimum detectable-permeation rate.
The required rate of flow may be obtained either by suitable control of the gas pressure at the inlet to the
permeation test cell or by providing a pump at the outlet of the analytical detector.
NOTE 1 The specific configuration is generally determined by the method of collection in combination with the techniques
used for detection of the test chemical or its component chemicals.
NOTE 2 Open-loop testing may provide different results from closed-loop testing due to differences in the system
configuration.
NOTE 3 This test method provides a choice of two minimum detectable-permeation rates for testing, either at 0,1 �g/cm /min
or 1,0 �g/cm /min.
8 © ISO 2001 – All rights reserved

ISO 6529:2001(E)
Dimensions in millimetres
Key
1 Loose cover 4Screws
2 Liquid compartment 5 Flow compartment
3 Test material
Exposed area of test material: 4,91 cm
Volume of flow compartment: 17,2 cm
Figure 4 — Alternative permeation test cell
ISO 6529:2001(E)
Key
1 Fresh collection medium 4Pump
2 Sampling side for collection medium 5 Sample analyser
3 Collection medium sample 6Waste
Figure 5 — Example configuration of open-loop permeation test equipment
6.4.1 Equipment for the collection medium, for either a gaseous or a liquid collection medium, capable of a
collection medium flow rate of five volume changes per minute for the collection chamber of the permeation test
cell.
6.4.2 Pump (if necessary).
6.4.3 Method of flow control.
6.4.4 Piping or tubing.
6.4.5 Permeation test cell (see 6.3).
6.4.6 Analytical detector, suitable for the test chemical.
6.5 Equipment for closed-loop permeation testing, consisting of the components listed in 6.5.1 to 6.5.6 and
shown inFigure6.
In closed-loop testing, select and configure equipment to provide the desired test system sensitivity in terms of the
minimum detectable permeation mass.
NOTE 1 The specific configuration is generally determined by method of collection in combination with the techniques used
for detection of the test chemical or its component chemicals.
NOTE 2 Closed-loop testing may provide different results from open-loop testing due to differences in the system
configuration.
NOTE 3 This test method provides a choice of two minimum detectable permeation masses for testing, either at 0,10 �g/cm
or 1,0 �g/cm .
10 © ISO 2001 – All rights reserved

ISO 6529:2001(E)
Key
1 Recycled collection medium 4Pump
2 Sampling side for collection medium 5 Sample analyser (non-destructive)
3 Collection medium sample 6 Analysed collection medium
Figure 6 — Example configuration for closed-loop permeation test equipment
6.5.1 Pump (if necessary).
6.5.2 Method of flow control.
6.5.3 Piping or tubing.
6.5.4 Permeation test cell (see 6.3).
6.5.5 Means for stirring or agitating the collection medium (when appropriate).
When used, agitation or stirring of the liquid collection medium at a rate of 0,1 r/s has been found to be satisfactory
for some permeation testing.
6.5.6 Analytical detector, suitable for the test chemical.
6.6 Stopwatch,or electronic timer.
6.7 Constant temperature chamber, or bath, used to maintain the permeation test cell within� 1,0 °Cof the
test temperature.
7 Sampling
7.1 Sampling procedure
Select test specimens from single material samples or individual protective clothing items consisting of either a
single layer or a composite of multiple layers that is representative of an actual protective clothing construction with
all layers arranged in proper order.
ISO 6529:2001(E)
If in the design of an item of protective clothing, different materials or thicknesses of material are specified at
different locations, select specimens from each location.
If in the design of an item of protective clothing, seams are claimed to offer the same protection as the base
materials, test additional specimens containing such seams.
Cut each material specimen so as to have a minimum cross-dimension consistent with the size of the test cell for
producing a uniform seal.
Test three specimens taken at random from each protective clothing material, composite, area (in the case of
[1]
heterogeneous design) or other condition. If desired, generate random specimens as described in ISO 2859-1 .
7.2 Preparation of test specimens
Condition each protective clothing specimen for a minimum of 24 h by exposure to a temperature of (21� 5) °C
and a relative humidity of (60�10)% accordingtoISO139.
NOTE If warranted, use other preconditioning options to assess possible degradation mechanisms of protective clothing.
7.3 Measurement of test specimen thickness and mass
Measure the thickness of each specimen to the nearest 0,02 mm in accordance with ISO 2286-3, ISO 5084, or
other method appropriate for the type of material being tested. Measure the mass of each specimen to the nearest
1g/m in accordance with ISO 2286-2, ISO 3801, or other method appropriate for the type of material being tested.
8 Procedure
8.1 Calibration
Calibrate the response of the analytical system to the test chemical, or its component chemicals, and where
appropriate, ensure that concentrations up to 25 % saturation of the collection medium may be determined.
Characterize the sensitivity of an open-loop system by its minimum detectable permeation rate. A method for
determining this value is presented in annex D.
Characterize the sensitivity of a closed-loop system by its minimum detectable mass permeated.
NOTE 1 Comparison of results requires specific information about the test cell, the procedures, and the analytical techniques.
Results obtained from closed-loop and open-loop testing may not be directly comparable.
NOTE 2 The sensitivity of the test method in detecting low permeation rates or amounts of the test chemical that permeate is
determined by the combination of the analytical technique and the collection system selected as well as the ratio of the material
specimen area to the collection medium volume or flow rate.
NOTE 3 A sensitive analytical technique permits quantitative detection of the test chemical in the collection medium at volume
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or mass fractions as low as 10 %.
NOTE 4 Higher ratios of material specimen area to collection medium volume or flow rate permit earlier detection of
breakthrough and detection of lower permeation rates because higher concentrations of the test chemical in the collection
medium will develop in a given time period, relative to those that would occur at lower ratios.
8.2 Preparation of test apparatus
8.2.1 Mount the first protective clothing material specimen in the permeation test cell (6.3.1) and assemble as
shown inFigure2.
12 © ISO 2001 – All rights reserved

ISO 6529:2001(E)
8.2.2 If the test is to be carried out at ambient temperature (see Note 1), test at a minimum temperature of 20 °C.
Ensure that the test cell is maintained at the selected ambient temperature, � 2 °C for at least 30 min prior to
testing and that the collection medium is at test temperature before adding it to the test cell.
NOTE 1 For the purposes of this test method, ambient temperatures are considered to be in the range of 20 °Cto27 °C.
NOTE 2 Persons performing this test or end-users interpreting this test should realize that permeation-resistance test results
are significantly influenced by temperature and that tested protective clothing materials can be used in non-ambient conditions.
For example, the skin temperature may be 35 °C and some protective clothing, particularly gloves, can be in intimate contact
with the skin.
8.2.3 If the test is to be carried out at a non-ambient temperature, place the assembled permeation test cell into
a constant-temperature chamber or bath at the test temperature. Maintain the permeation test cell at the non-
ambient temperature for at least 30 min before the testing proceeds further.
8.2.4 Load the collection medium into the collection chamber of the permeation test cell (the chamber to which
the normal inside surface of the material specimen is exposed). Depending upon the combination of the analytical
technique and the collection medium selected, configure the test equipment as appropriate (see Figures 5 and 6).
8.2.5 If the test is to be carried out at a non-ambient temperature and the collection medium is a liquid, bring the
medium to the test temperature before adding it to the test cell.
8.2.6 Stir, circulate, or flow the collection medium continuously.
NOTE The purpose of agitating the collection medium is twofold:
a) to ensure that it is homogeneous for sampling and analytical purposes, and
b) to prevent or minimize concentration boundary layers of permeant at the interface of the clothing material and the collection
medium.
The degree of agitation necessary to achieve these objectives is dependent on the permeation rate and the relative solubilities
of the te
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