prEN ISO 6529 rev

SLOVENSKI STANDARD
oSIST prEN ISO 6529 :2011
01-oktober-2011
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Protective clothing -- Protection against chemicals -- Determination of resistance of
protective clothing materials to permeation by liquids and gases
Schutzkleidung - Schutz gegen Chemikalien - Bestimmung des Widerstands von
Schutzkleidungsmaterialien gegen die Permeation von Flüssigkeiten und Gasen
Vêtements de protection - Protection contre les produits chimiques - Détermination de la
résistance des matériaux utilisés pour la confection des vêtements de protection à la
perméation par des liquides et des gaz
Ta slovenski standard je istoveten z: prEN ISO 6529 rev
ICS:
13.340.10 Varovalna obleka Protective clothing
oSIST prEN ISO 6529 :2011 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN ISO 6529 :2011
oSIST prEN ISO 6529 :2011
EUROPEAN STANDARD
DRAFT
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2011
ICS 13.340.10 Will supersede EN ISO 6529:2001
English Version
Protective clothing - Protection against chemicals -
Determination of resistance of protective clothing materials to
permeation by liquids and gases (ISO/DIS 6529:2011)
Vêtements de protection - Protection contre les produits Schutzkleidung - Schutz gegen Chemikalien - Bestimmung
chimiques - Détermination de la résistance des matériaux des Widerstands von Schutzkleidungsmaterialien gegen
utilisés pour la confection des vêtements de protection à la die Permeation von Flüssigkeiten und Gasen (ISO/DIS
perméation par des liquides et des gaz (ISO/DIS 6529:2011)
6529:2011)
This draft European Standard is submitted to CEN members for parallel enquiry. It has been drawn up by the Technical Committee
CEN/TC 162.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations which
stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other language
made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are aware and to
provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without notice and
shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN ISO 6529 rev:2011: E
worldwide for CEN national Members.

oSIST prEN ISO 6529 :2011
prEN ISO 6529:2011 (E)
Contents Page
Foreword .3

oSIST prEN ISO 6529 :2011
prEN ISO 6529:2011 (E)
Foreword
This document (prEN ISO 6529:2011) has been prepared by Technical Committee ISO/TC 94 “Personal
safety - Protective clothing and equipment” in collaboration with Technical Committee CEN/TC 162 “Protective
clothing including hand and arm protection and lifejackets” the secretariat of which is held by DIN.
This document is currently submitted to the parallel Enquiry.
This document will supersede EN ISO 6529:2001.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association, and supports essential requirements of EU Directive.
For relationship with EU Directive, see informative Annex ZA, which is an integral part of this document.
Endorsement notice
The text of ISO/DIS 6529:2011 has been approved by CEN as a prEN ISO 6529:2011 without any
modification.
oSIST prEN ISO 6529 :2011
oSIST prEN ISO 6529 :2011
DRAFT INTERNATIONAL STANDARD ISO/DIS 6529
ISO/TC 94/SC 13 Secretariat: SNV
Voting begins on Voting terminates on

2011-07-14 2011-12-14
INTERNATIONAL ORGANIZATION FOR STANDARDIZATION  •  МЕЖДУНАРОДНАЯ ОРГАНИЗАЦИЯ ПО СТАНДАРТИЗАЦИИ  •  ORGANISATION INTERNATIONALE DE NORMALISATION

Protective clothing — Protection against chemicals —
Determination of resistance of protective clothing materials to
permeation by liquids and gases
Vêtements de protection — Protection contre les produits chimiques — Détermination de la résistance des
matériaux utilisés pour la confection des vêtements de protection à la perméation par des liquides et des gaz
[Revision of second edition (ISO 6529:2001)]
ICS 13.340.10
ISO/CEN PARALLEL PROCESSING
This draft has been developed within the International Organization for Standardization (ISO), and
processed under the ISO-lead mode of collaboration as defined in the Vienna Agreement.
This draft is hereby submitted to the ISO member bodies and to the CEN member bodies for a parallel
five-month enquiry.
Should this draft be accepted, a final draft, established on the basis of comments received, will be
submitted to a parallel two-month approval vote in ISO and formal vote in CEN.

In accordance with the provisions of Council Resolution 15/1993 this document is circulated in
the English language only.
Conformément aux dispositions de la Résolution du Conseil 15/1993, ce document est
distribué en version anglaise seulement.

To expedite distribution, this document is circulated as received from the committee
secretariat. ISO Central Secretariat work of editing and text composition will be undertaken at
publication stage.
Pour accélérer la distribution, le présent document est distribué tel qu'il est parvenu du
secrétariat du comité. Le travail de rédaction et de composition de texte sera effectué au
Secrétariat central de l'ISO au stade de publication.

THIS DOCUMENT IS A DRAFT CIRCULATED FOR COMMENT AND APPROVAL. IT IS THEREFORE SUBJECT TO CHANGE AND MAY NOT BE
REFERRED TO AS AN INTERNATIONAL STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS BEING ACCEPTABLE FOR INDUSTRIAL, TECHNOLOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT, WITH THEIR COMMENTS, NOTIFICATION OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPORTING DOCUMENTATION.
©  International Organization for Standardization, 2011

oSIST prEN ISO 6529 :2011
ISO/DIS 6529
Copyright notice
This ISO document is a Draft International Standard and is copyright-protected by ISO. Except as permitted
under the applicable laws of the user’s country, neither this ISO draft nor any extract from it may be
reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic,
photocopying, recording or otherwise, without prior written permission being secured.
Requests for permission to reproduce should be addressed to either ISO at the address below or ISO’s
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E-mail copyright@iso.org
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Reproduction may be subject to royalty payments or a licensing agreement.
Violators may be prosecuted.
ii © ISO 2011 – All rights reserved

oSIST prEN ISO 6529 :2011
ISO/DIS 6529
Contents Page
Foreword .v
Introduction.vi
1 Scope.1
2 Normative references (Note to editor – none of the references to other standards are
normative) .2
3 Terms and definitions .2
4 Principle .5
5 Choice of analytical technique and collection medium .5
5.1 General .5
5.2 Gaseous collection media .6
5.3 Liquid collection media.6
5.4 Other collection media.6
6 Apparatus.6
7 System Configuration .8
7.1 Open-Loop .8
7.2 Closed-Loop.9
8 Detection .10
8.1 Frequency of analysis.10
8.2 Analytical Methods.11
9 Sampling .12
9.1 Sampling procedure.12
9.2 Preparation of test specimens .12
9.3 Measurement of test specimen thickness and mass .12
10 Procedure.13
10.1 Calibration.13
10.2 Validation .13
10.3 Preparation of test apparatus .13
10.4 Method A — Liquid chemicals with continuous contact.14
10.5 Method B — Gaseous chemicals with continuous contact .15
10.6 Method C — Liquid or gaseous chemical with intermittent contact.18
10.7 Calculation of results.18
10.8 Visual assessment of test specimen.22
11 Report .23
Annex A (informative) Recommended list of chemicals for comparing permeation resistance of
protective clothing materials .25
Annex B (informative) Sources of permeation test cells and permeation test cell parts.28
Annex C (informative) Designs and Specifications of Commonly-Used Permeation Test Cells.29
Annex D (informative) Suggested procedures for calibrating and measuring the sensitivity of
permeation-test systems .33
Annex E (informative) Suggested method for the determination of air impermeability.37
Annex F (informative) Testing the permeation resistance of seams and closures.39
Annex ZA (informative) Relationship between this European Standard and the Essential
Requirements of EC Directive 89/686/EEC .42
oSIST prEN ISO 6529 :2011
ISO/DIS 6529
Bibliography. 43

iv © ISO 2011 – All rights reserved

oSIST prEN ISO 6529 :2011
ISO/DIS 6529
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6529 was prepared by Technical Committee ISO/TC 94, Personal safety - Protective clothing and
equipment, Subcommittee SC 13 and by Technical Committee CEN/TC 162, Protective clothing including
hand and arm protection and lifejackets in collaboration.
This third edition cancels and replaces the second edition (EN ISO 6529:2001), which has been technically
revised.
This European Standard has been prepared under a mandate given to CEN by the European Commission
and the European Free Trade Association, to support Essential Requirements of EU Directive 89/686/EEC

For relationship with the EU directive, see informative Annex ZA which is an integral part of this document.
oSIST prEN ISO 6529 :2011
ISO/DIS 6529
Introduction
Workers involved in the production, use, transportation, and emergency response with liquid and gaseous
chemicals can be exposed to numerous compounds capable of causing harm upon contact with the human
body. The deleterious effects of these chemicals can range from acute trauma such as skin irritation and burn
to chronic degenerative disease, such as cancer. Since engineering controls may not eliminate all possible
exposures, attention is often placed on reducing the potential for direct skin contact through the use of
protective clothing that resists permeation, penetration and degradation.
The test methods described in this standard are intended to be used to evaluate the barrier effectiveness of
materials used for protective clothing (see Note) against ingress by liquid or gaseous chemicals. Options are
provided for conducting this testing under both conditions of continuous or intermittent contact with the
chemicals.
These test methods provide various options for reporting test results in terms of breakthrough time,
permeation rate and cumulative permeation to allow a comparison of protective clothing material permeation
resistance. These parameters are key measures of the effectiveness of a clothing material to act as a barrier
to the test chemical. Such information is used in the comparison of clothing materials during the process of
selecting clothing for protection from hazardous chemicals. Long breakthrough times, low permeation rates
and low cumulative permeation mass are characteristic of high level barrier materials.
Resistance to penetration by liquid chemicals should be determined by using ISO 6530 while resistance to
penetration by liquid chemicals under pressure should be determined by using ISO 13994. These International
Standards are listed in the Bibliography.
It has been assumed in the drafting of this International Standard that the execution of its provisions will be
entrusted to appropriately qualified and experienced people with a sound understanding of analytical
chemistry. Appropriate precautions should be taken when carrying out this type of testing in order to avoid
injury to health and contamination of the environment.
NOTE Finished items of protective clothing include gloves, arm shields, aprons, suits, hoods, boots, etc. The phrase
“specimens from finished items” encompasses seamed and other discontinuous regions as well as the usual continuous
regions of protective clothing items.

vi © ISO 2011 – All rights reserved

oSIST prEN ISO 6529 :2011
DRAFT INTERNATIONAL STANDARD ISO/DIS 6529

Protective clothing — Protection against chemicals —
Determination of resistance of protective clothing materials to
permeation by liquids and gases
1 Scope
This International Standard describes laboratory test methods to determine the resistance of materials used in
protective clothing to permeation by liquid or gaseous chemicals under the conditions of either continuous or
intermittent contact.
Method A is applicable to the testing of liquid chemicals, either volatile or soluble in water, expected to be in
continuous contact with the protective clothing material.
Method B is applicable to the testing of gaseous chemicals expected to be in continuous contact with the
protective clothing material.
Method C is applicable to the testing of liquid chemicals, either volatile or soluble in water, expected to be in
intermittent contact with the protective clothing material.
These test methods are only suitable for the testing of air-impermeable protective clothing materials (see
Note 1). They assess the permeation resistance of the protective clothing material under laboratory
conditions in terms of breakthrough time, permeation rate, and cumulative permeation. These test methods
also enable observations to be made of the effects of the test liquid on the protective clothing material under
test.
NOTE 1 In many cases the manufacturer of the protective clothing material will be able to advise whether their product
is air-impermeable. If this information is not available the procedure in Annex E may be used to evaluate air-
impermeability.
These test methods are only suitable for measuring permeation by liquids and gases. Permeation by solid
challenge chemicals is beyond the scope of this standard.
NOTE 2 It can be difficult or impossible to normalize the results of permeation tests carried out against solid challenge
chemicals. The normalised rate of permeation is dependent on the area of fabric exposed to the challenge chemical. In
the case of solids this will, in turn, depend on factors such as particle size, size distribution, particle shape and packing
considerations
These test methods address only the performance of materials or certain material constructions (e.g. seams)
used in protective clothing. These test methods do not address the design, overall construction and
components, or interfaces of garments or other factors which may affect the overall protection offered by the
protective clothing.
It is emphasized that these tests do not necessarily simulate conditions to which clothing materials are likely to
be exposed in practice. In most cases the conditions of the permeation test will be far more challenging than
expected workplace conditions. The use of test data should therefore be restricted to broad comparative
assessment of such material according to their permeation-resistance characteristics.
oSIST prEN ISO 6529 :2011
ISO/DIS 6529
2 Normative references (Note to editor – none of the references to other standards
are normative)
The following normative documents contain provisions which, through reference in this text, constitute
provisions of this International Standard. For dated references, subsequent amendments to, or revisions of,
any of these publications do not apply. However, parties to agreements based on this International Standard
are encouraged to investigate the possibility of applying the most recent editions of the normative documents
indicated below. For undated references, the latest edition of the normative document referred to applies.
Members of ISO and IEC maintain registers of currently valid International Standards.
3 Terms and definitions
For the purposes of this International Standard, the following terms and definitions apply.
3.1
analytical technique
method of quantifying the amount of permeated chemical in the collection medium
NOTE Such methods are often specific to individual chemical and collection-medium combinations.
EXAMPLES Applicable analytical techniques can include ultraviolet (UV) and infrared (IR) spectrophotometry, mass
spectrometry, pH measurement, ion chromatography, conductimetry, colorimetry, atmospheric analytical detector tubes
and radionuclide tagging/detection counting. Although gas- and liquid-chromatography are separation techniques rather
than detection methods they can be used in conjunction with suitable detectors to quantify the amount of permeated
chemical in the collection medium.
3.2
breakthrough detection time
elapsed time measured from the start of the test to the sampling time that immediately precedes the sampling
time at which the test chemical is first detected
See Figure 1.
NOTE The breakthrough detection time is dependent on the sensitivity of the method and the frequency of sampling
(the interval between sampling times).
3.3
closed-loop
refers to a testing mode in which the collection medium volume is fixed and continuously circulated or recycled
NOTE 1 The collection medium volume may change slightly from sampling without replacement of the sampled
collection medium.
NOTE 2 The closed-loop collection medium need not necessarily be completely physically enclosed. For example,
liquid collection media may be open to the air in an expansion vessel or constant-pressure header vessel.
3.4
collection medium
liquid or gas on the inner “clean” side of the test sample in which any permeated chemical is collected.
3.5
contact time
in an intermittent contact (Method C) test, the duration that the challenge-side chamber of the permeation cell
contains test chemical during each cycle
3.6
cumulative permeation mass
total amount of chemical that permeates during a specified time from the start of the test
2 © ISO 2011 – All rights reserved

oSIST prEN ISO 6529 :2011
ISO/DIS 6529
NOTE 1 Quantification of cumulative permeation enables the comparison of permeation behaviour under intermittent
contact conditions with analogous data for tests carried out under continuous-contact conditions.
NOTE 2 The measurement of cumulative permeation may depend on the sensitivity of the permeation-test system.
3.7
cycle time
in an intermittent contact (Method C) test, the interval of time from the start of one contact period to the start of
the next contact period
NOTE 1 The breakthrough detection time for a method sensitivity of 0,05 μg/cm2/min is 23 min but would be reported
at 20 min, which corresponds to the last sampling time preceding the test. The normalized breakthrough detection time at
a normalization permeation rate of 0,1 μg/cm2/min is 33 min, but similarly would be reported at 28 min, which corresponds
to the preceding sampling time. The steady-state permeation rate is approximately 0,15 μg/cm2/min.
NOTE 2 The cumulative permeated mass over a 60 minute period is equal to the area of the shaded region under the
graph
Figure 1 — Schematic permeation graph showing actual and normalized breakthrough times and
cumulative permeation mass
3.8
degradation
deleterious change in one or more physical properties of a protective clothing material
NOTE Deleterious changes can be manifest as either an increase or decrease in a physical property. For example if
the protective clothing material has been embrittled an increase in puncture resistance may be observed
oSIST prEN ISO 6529 :2011
ISO/DIS 6529
3.9
minimum detectable mass permeated
smallest mass of test chemical that is detectable with the complete permeation-test system
NOTE This value is not necessarily the intrinsic limit of detection for the analytical instrument.
3.10
minimum detectable permeation rate
lowest rate of permeation that is measurable with the complete permeation-test system
NOTE This value is not necessarily the intrinsic limit of detection for the analytical instrument.
3.11
normalization permeation rate
permeation rate used for determining the normalized breakthrough detection time
2 2
NOTE This test method provides two choices of normalization permeation rates: 0,1 μg/cm /min or 1,0 μg/cm /min.
3.12
normalized breakthrough detection time
time at which the permeation rate reaches the normalization permeation rate
See Figure 1.
3.13
open-loop
testing mode in which fresh collection medium flows continuously through the collection chamber of the test
cell and is not reused or recycled
3.14
penetration
flow of a chemical through closures, porous materials, seams and holes or other imperfections in a protective
clothing material on a non-molecular level
3.15
permeation
process by which a chemical moves through a protective clothing material on a molecular level
NOTE Permeation involves
a) sorption of molecules of the chemical into the contacted (outside) surface of a material,
b) diffusion of the sorbed molecules in the material, and
c) desorption of the molecules from the opposite (inside) surface of the material into the collection medium.
3.16
permeation mass
quantity of test chemical that passes through the protective clothing material within a given time
3.17
permeation rate
quantity of test chemical that passes through a given exposed surface area of protective clothing material in a
given time. Permeation rate is usually expressed in the units micrograms per square centimetre per minute
-2 -1
(μgcm min )
3.18
protective clothing material
any material or combination of materials used in an item of clothing for the purpose of isolating parts of the
body from a potential hazard
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oSIST prEN ISO 6529 :2011
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3.19
purge time
In an intermittent contact test (Method C) this is the time immediately following the termination of the contact
time when the test chemical is removed from the challenge-side chamber and air or nitrogen is blown over the
outside surface of the protective clothing material
3.20
steady-state permeation rate
constant rate of permeation that occurs after breakthrough when the chemical contact is continuous and all
forces affecting permeation have reached equilibrium
NOTE Steady-state permeation may not be achieved during the period for which permeation testing is conducted.
3.21
test chemical or challenge chemical
liquid or gas that is used to challenge the protective clothing material specimen
NOTE The liquid or gas may be either one component (i.e. a neat liquid or gas) or have several components (i.e. a
mixture or solution).
4 Principle
The protective clothing material specimen acts as a partition between one chamber of a permeation test cell,
which contains the test chemical, and another chamber, which contains the collection medium.
The test chemical may be either a liquid or a gas. The protective clothing material specimen may contact the
test chemical either continuously or intermittently depending on the choice of the method used.
The collection medium, which may be liquid or gas, is (either periodically or continuously) analysed
quantitatively for its concentration of the challenge chemical. The amount of that chemical that has passed
through the material specimen is recorded as a function of time after its initial contact with the material.
Different test configurations may be used depending on the choice of the test chemical, collection medium and
conditions of the test.
The breakthrough detection time, normalized breakthrough detection time, permeation rate, and cumulative
permeation of the test chemical may be determined by either graphical representation or appropriate
calculations, or both,
A group of chemicals, representing a range in chemical properties, which can be used to compare the
permeation resistance is given in annex A.
5 Choice of analytical technique and collection medium
5.1 General
The combination of the analytical technique and the collection medium shall be selected to optimise sensitivity
for the detection of the test chemical. Wherever possible the collection medium shall represent actual
occupational conditions.
NOTE: When in use, the inner face of chemical protective clothing fabrics is usually exposed to either air inside the
garment, aqueous solutions from perspiration, or both. The use of, for example, ammonia or methane as collection media
would not be appropriate since neither will be present inside the protective clothing during normal use. Use of such
collection media may also affect the test results since these substances may permeate the test specimen from the
collection side of the test cell.
oSIST prEN ISO 6529 :2011
ISO/DIS 6529
5.2 Gaseous collection media
A gaseous collection medium is usually used under continuous flow conditions for the collection of permeating
molecules that are capable of vaporizing from the test liquid under the conditions of the test both quantitatively
and in sufficient quantities for analysis. The gaseous collection medium shall be a gas or gas mixture which
does not interfere with the detection of the test chemical and does not itself permeate or degrade the fabric
under test. The quality of the gas supply shall be of sufficient consistency over the duration of the test that
changes do not interfere with detection of the test chemical.
EXAMPLES Nitrogen or dry air.
NOTE 1 If ambient air is used as a collection medium care should be taken to ensure that the moisture content does not
vary significantly during the test
NOTE 2 Helium and hydrogen may not be a suitable collection media since these gases can permeate some plastics
and elastomers, including gaskets and sealing-washers
NOTE 3 If reactive gases such as oxygen are used as collection media appropriate safety measures should be adopted
5.3 Liquid collection media
A liquid collection medium is usually used for the collection of permeated molecules of low volatility that are
soluble in the collecting medium under the conditions of the test in sufficient quantities for analysis. The liquid
collection medium shall be either water, an aqueous solution or another liquid which does not interfere with
the detection of the test chemical and does not itself permeate or degrade the fabric under test.
NOTE It should be recognised that there are circumstances under which the above criteria are mutually exclusive. For
example, when testing a PVC fabric for resistance to permeation by an involatile isocyanate it will be found that the
challenge chemical is insoluble in aqueous collection media and that the test fabric is readily permeated or degraded by
virtually all non-aqueous liquids. Under such circumstances testing is, unfortunately, not possible.
If there is any doubt as to whether a liquid collection medium will degrade or permeate a test fabric then the
test fabric shall first be tested for permeation resistance to the collection medium. In many cases this will be
possible by open-loop testing using a gaseous collection medium. If any permeation or degradation is
observed over an 8 hour exposure then the collection medium shall be deemed to be inappropriate.
5.4 Other collection media
Other collection medium such as solid sorbents may be used provided that suitable collection efficiencies are
demonstrated for the test chemical being used. The efficiency of such collection media shall, wherever
possible, be validated by comparison to similar tests using either a gaseous or liquid collection medium.
NOTE Care should be taken to ensure that there is complete and continuous contact between the inner face of the
fabric and the collection medium. This is particularly important when considering solid particulate collection media since
the degree of contact will depend on particle size.
6 Apparatus
6.1 Thickness gauge, suitable for measuring thickness to the nearest 0,02 mm, as specified in ISO 2286-
3, ISO 5084 or similar to determine the thickness of each protective clothing material specimen tested.
NOTE The purpose of this gauge is not to give a definitive measurement of fabric thickness but to highlight any inter-
sample thickness variations. Permeation can be extremely sensitive to very minor variations in sample thickness.
6.2 Analytical balance, capable of being read to the nearest 0,01 g.
6 © ISO 2011 – All rights reserved

oSIST prEN ISO 6529 :2011
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Note The level of precision stated above is that necessary for measuring the mass per unit area of test fabrics. If
mass is used in any procedures for the calibration of detection equipment it may be necessary to have a balance capable
of being read to the nearest 0.0001g
6.3 Permeation test cell, consisting of a two-chambered cell for contacting the specimen with the test
chemical on the specimen's normal outside surface (clothing exterior) and with a collection medium on the
specimen's normal inside surface (clothing interior). Test cells conforming to one of the diagrams and
associated descriptions in Annex B are suitable but other designs are acceptable provided that they meet the
following criteria:
The area of the outer surface of the fabric that is exposed to the test chemical shall coincide with the area of
the inner surface of the fabric which is exposed to the collection medium.
The capacity of the challenge side of the apparatus shall be sufficiently large that volume and/or concentration
of test chemical are not significantly diminished by permeation
The challenge side of the apparatus shall be so designed that the mass or flow of the test chemical does not
apply undue force to the fabric under test.
The collection side of the apparatus shall be so designed that the mass or flow of the collection medium does
not apply undue force to the fabric under test.
The difference in pressure between the two surfaces of the test sample shall not exceed 5000Pa
NOTE 1 Undue force may cause the fabric to stretch thereby rendering it thinner and less resistant to permeation
NOTE 2 If the test chemical or collection medium flows into and out of the apparatus the pipes into and out of the cell
should be of sufficient internal cross-sectional area that no significant pressure is generated under flow conditions.
The apparatus shall be so designed that the outer surface of the test sample is in complete contact with the
test chemical and the inner surface is in complete contact with the collection medium.
NOTE 3 Care should be taken that air bubbles in liquid collection media and liquid test chemicals do not rest against the
fabric under test
The collection side of the apparatus shall be so designed that the collection medium is thoroughly mixed and
that aliquots of collection medium analysed for the presence of test chemical are representative of the whole
of the collection medium.
NOTE 4 This may be achieved in a number of ways including physical agitation or turbulent flow of the collection
medium.
The materials of construction of the apparatus shall be such that they do not alter the nature or composition of
the test chemical, the collection medium or any of the test chemical that has permeated through the fabric
The apparatus shall be so designed that the only way that test chemical can get into the collection medium is
by first permeating through the test fabric.
NOTE 5 Care should be taken to ensure that test chemical cannot leak out of the challenge side of the apparatus, flow
around the edge of the test sample and then leak into the collection side. This is particularly likely to happen by capillary
action if one or both surfaces of the test fabric are made from a woven or non-woven textile.
6.4 Equipment for the collection medium, for either a gaseous or a liquid collection medium, capable of a
collection medium flow rate of five volume changes per minute for the collection chamber of the permeation
test cell.
6.4.1 Pump (if necessary).
6.4.2 Method of flow control.
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6.4.3 Piping or tubing.
6.4.4 Permeation test cell (see 6.3).
6.4.5 Analytical detector, suitable for the test chemical.
6.5 Equipment for open-loop or closed-loop permeation testing, consisting of the components shown
in Figures 2 and 3.
6.5.1 Pump (if necessary).
6.5.2 Method of flow control.
6.5.3 Piping or tubing.
6.5.4 Permeation test cell
6.5.5 Means for stirring or agitating the collection medium (when appropriate).
Note When used, agitation or stirring of the liquid collection medium at a rate of 0,1 r/s has been found to be
satisfactory for some permeation testing.
6.5.6 Analytical detector, suitable for the detection of the test chemical in the collection medium.
6.6 Stopwatch, or electronic timer.
6.7 Constant temperature chamber, bath or room, used to maintain the permeation test cell
within ± 1,0 °C of the nominal test temperature.
NOTE 1 For reasons of safety, chemical permeation testing is often carried out in laboratory fume-cupboards or in
association with other fume-extraction equipment. If air conditioning equipment is used to maintain the test temperature
air flowing into the laboratory, to replace air and fumes extracted, may first need to be conditioned to the appropriate
temperature.
If a water-bath is used to maintain the test temperature then steps shall be taken to ensure that water does
not leak into either side of the test cell or wick into any internal or external textile component of the test
specimen.
NOTE 2 This requirement may be achieved by first enclosing the test cell in a waterproof bag before the bagged cell is
then immersed in the water-bath.
7 System Configuration
The apparatus shall be arranged in either the open-loop or closed-loop configuration.
NOTE 1 The specific configuration is generally determined by method of collection in combination with the techniques
used for detection of the test chemical or its component chemicals.
NOTE 2 Closed-loop testing may provide different results from open-loop testing as a consequence of differences in the
system configuration and collection media. The rate of desorption of permeated molecules from the inner face of the test
chemical can depend on the nature of the collection medium.
7.1 Open-Loop
In the open-loop configuration the collection medium flows (see NOTE 1) from a supply reservoir, through the
collection side of the permeation test cell, to a detector where it is analysed for the presence of test chemical.
After analysis the collection medium flows to waste.
8 © ISO 2011 – All rights reserved

oSIST prEN ISO 6529 :2011
ISO/DIS 6529
NOTE 1 The collection may flow under gravity (in the case of a liquid) under pressure (in the case of a gas) or may flow
by the action of a suitable pump (as illustrated below)
NOTE 2 Awaiting text to describe how to control flow of liquid collection media under gravity
NOTE 3 The open-loop configuration is typically used with in conjunction with gaseous collection media when testing
against volatile organic challenge chemicals
The apparatus shall be selected, designed and configured so as optimise the test sensitivity. For the open-
loop configuration this is usually best achieved by keeping the area of fabric exposed to the test chemical as
large as practicable. In this regard, larger models of test-cell tend to give higher sensitivity.
The flow-rate of collection medium through the collection side of the test cell shall be sufficiently high that any
molecules of test chemical permeating through the test fabric are rapidly mixed into the collection medium.
The minimum flow-rate required to achieve this will depend on the exact design of the permeation test cell. A
flow-rate equal to five times the volume of the collection side of the test cell per minute shall be used unless,
for a specific design of test cell, it has previously been demonstrated that a lower flow-rate gives equally good
mixing. In such cases mixing shall be deemed sufficient if a further increase in the degree of mixing does not
alter the measured breakthrough time or steady-state permeation rate.
NOTE 4 Lower flow-rates result in lower dilution of permeated chemical and thereby increase minimum detection levels

Key
1 Fresh collection medium 4 Pump
2 Sampling side for collection medium 5 Sample analyser
3 Collection medium sample 6 Waste
Figu
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