EN ISO 16588:2003

SLOVENSKI STANDARD
01-februar-2004
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NURPDWRJUDILMH,62
Water quality - Determination of six complexing agents - Gas-chromatographic method
(ISO 16588:2002)
Wasserbeschaffenheit - Bestimmung von sechs Komplexbildnern -
Gaschromatographisches Verfahren (ISO 16588:2002)
Qualité de l'eau - Dosage des agents complexants - Méthode par chromatographie en
phase gazeuse (ISO 16588:2002)
Ta slovenski standard je istoveten z: EN ISO 16588:2003
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 16588
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2003
ICS 13.060.50
English version
Water quality - Determination of six complexing agents - Gas-
chromatographic method (ISO 16588:2002)
Qualité de l'eau - Dosage des agents complexants - Wasserbeschaffenheit - Bestimmung von sechs
Méthode par chromatographie en phase gazeuse (ISO Komplexbildnern - Gaschromatographisches Verfahren
16588:2002) (ISO 16588:2002)
This European Standard was approved by CEN on 3 November 2003.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United
Kingdom.
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COMITÉ EUROPÉEN DE NORMALISATION
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Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2003 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16588:2003 E
worldwide for CEN national Members.

Foreword
The text of ISO 16588:2002 has been prepared by Technical Committee ISO/TC 147 "Water
quality” of the International Organization for Standardization (ISO) and has been taken over as
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by May 2004, and conflicting national standards
shall be withdrawn at the latest by May 2004.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium, Czech
Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and
the United Kingdom.
Endorsement notice
The text of ISO 16588:2002 has been approved by CEN as EN ISO 16588:2003 without any
modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA
(normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions of
any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications, indicated
by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 3696 1987 Water for analytical laboratory use - EN ISO 3696 1995
Specification and test methods
ISO 5667-1 1980 Water quality - Sampling - Part 1: EN 25667-1 1993
Guidance on the design of sampling
programmes
ISO 5667-2 1991 Water quality - Sampling - Part 2: EN 25667-2 1993
Guidance on sampling techniques
INTERNATIONAL ISO
STANDARD 16588
First edition
2002-11-01
Water quality — Determination of six
complexing agents — Gas-chromatographic
method
Qualité de l'eau — Dosage de six agents complexants — Méthode par
chromatographie en phase gazeuse

Reference number
ISO 16588:2002(E)
ISO 16588:2002(E)
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©
ii ISO 2002 – All rights reserved

ISO 16588:2002(E)
Contents Page
1 Scope . 1
2 Normative references . 1
3 Principle . 2
4 Interferences . 2
5 Reagents . 2
6 Apparatus . 4
7 Sampling and sample stabilization . 4
8 Procedure . 5
9 Calibration . 7
10 Expression of results . 9
11 Test report . 9
Annex
A Examples of columns, chromatograms and mass spectra. 10
Bibliography. 12
©
ISO 2002 – All rights reserved iii

ISO 16588:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 16588 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee
SC 2, Physical, chemical and biochemical methods.
Annex A of this International Standard is for information only.
©
iv ISO 2002 – All rights reserved

ISO 16588:2002(E)
Introduction
It is essential that the test described in this International Standard be carried out by suitably qualified staff.
It should be investigated whether and to what extent particular problems will require the specification of additional
conditions.
©
ISO 2002 – All rights reserved v

INTERNATIONAL STANDARD ISO 16588:2002(E)
Water quality — Determination of six complexing agents —
Gas-chromatographic method
WARNING — Persons using this International Standard should be familiar with normal laboratory practice.
This standard does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance with
any national regulatory conditions.
1 Scope
This International Standard specifies a method for the determination of the water-soluble organic complexing agents
listed in Table 1 in the concentration range from 0,5µg/l to 200µg/l, if a sample volume between 50 ml and 100 ml is
used. The concentration range may change if diluted solutions are analysed. The method is applicable to drinking,
ground, surface and waste water.
Table 1 — Complexing agents determinable by this method
a
No. Name Composition Molecular mass CAS number
1EDTA — ethylenedinitrilotetraacetic acid C H O N 292,25 60-00-4
10 16 8 2
2NTA — nitrilotriacetic acid C H O N 191,14 139-13-9
6 9 6
3DTPA — diethylenetrinitrilopentaacetic acid C H O N 393,35 67-43-6
14 23 10 3
4MGDA — methylglycinediacetic acid C H O N 205,17 29578-05-0
7 11 6
5ββ-ADA — -alaninediacetic acid C H O N 205,17 6245-75-6
7 11 6
61,3-PDTA — 1,3-propylenedinitrilotetraacetic acid C H O N 306,27 1939-36-2
11 18 8 2
a
CAS: Chemical Abstracts System
In waste water analysis, it is recommended that a smaller sample volume, e.g. 5ml or 10ml, be used in order to
reduce matrix effects.
The adsorption of the six complexing agents on solid materials is negligibly low.
Other complexing agents of similar composition may also be determined using this method, provided they behave
similarly during sample pretreatment, derivatization and gas chromatography. This shall be checked in each
individual case.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 5667-1:1980, Water quality — Sampling — Part 1: Guidance on the design of sampling programmes
ISO 5667-2:1991, Water quality — Sampling — Part 2: Guidance on sampling techniques
©
ISO 2002 – All rights reserved 1

ISO 16588:2002(E)
3Principle
A test sample is stabilized with formaldehyde and evaporated to dryness. Hydrochloric or formic acid is added and
the sample again evaporated to dryness. The complexing agents are then esterified to the n-propyl, iso-propyl or
n-butyl esters. Water is added and the esters are extracted with n-hexane, separated and identified by gas
chromatography, and determined quantitatively with a nitrogen-sensitive detector or by mass spectrometry.
For the determination of EDTA, DTPA, and 1,3-PDTA, 1,2-propylenedinitrotetraacetic acid (1,2-PDTA)
...