EN ISO 12193:2004

SLOVENSKI STANDARD
01-maj-2004
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD'RORþHYDQMHVYLQFD]GLUHNWQRDWRPVNR
DEVRUSFLMVNRVSHNWURPHWULMR]JUDILþQRNLYHWR,62
Animal and vegetable fats and oils - Determination of lead by direct graphite furnace
atomic absorption spectroscopy (ISO 12193:2004)
Tierische und pflanzliche Fette und Öle - Bestimmung von Blei durch direkte Graphitofen
-Atomabsorptionsspektrometrie (ISO 12193:2004)
Corps gras d'origines animale et végétale - Détermination de la teneur en plomb par
spectrométrie d'absorption atomique directe avec four en graphite (ISO 12193:2004
Ta slovenski standard je istoveten z: EN ISO 12193:2004
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 12193
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2004
ICS 67.200.10 Supersedes EN ISO 12193:1999
English version
Animal and vegetable fats and oils - Determination of lead by
direct graphite furnace atomic absorption spectroscopy (ISO
12193:2004)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung von
de la teneur en plomb par spectrométrie d'absorption Blei durch direkte Graphitofen-
atomique directe avec four en graphite (ISO 12193:2004 Atomabsorptionsspektrometrie (ISO 12193:2004)
This European Standard was approved by CEN on 19 February 2004.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12193:2004: E
worldwide for CEN national Members.

CORRECTED  2004-02-25
Foreword
This document (EN ISO 12193:2004) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 307 "Oilseeds,
vegetable and animal fats and oils and their by-products - Methods of sampling and analysis",
the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by August 2004, and conflicting national
standards shall be withdrawn at the latest by August 2004.
This document supersedes EN ISO 12193:1999.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 12193:2004 has been approved by CEN as EN ISO 12193:2004 without any
modifications.
INTERNATIONAL ISO
STANDARD 12193
Second edition
2004-02-01
Animal and vegetable fats and oils —
Determination of lead by direct graphite
furnace atomic absorption spectroscopy
Corps gras d'origines animale et végétale — Détermination de la teneur
en plomb par spectrométrie d'absorption atomique directe avec four en
graphite
Reference number
ISO 12193:2004(E)
©
ISO 2004
ISO 12193:2004(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.

©  ISO 2004
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2004 – All rights reserved

ISO 12193:2004(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 12193 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This second edition cancels and replaces the first edition (ISO 12193:1994), which has been technically
revised.
INTERNATIONAL STANDARD ISO 12193:2004(E)

Animal and vegetable fats and oils — Determination of lead by
direct graphite furnace atomic absorption spectroscopy
1 Scope
This International Standard specifies a method for the determination of trace amounts (> 0,001 mg/kg) of lead
in all types of crude or refined edible oils and fats.
2 Principle
The oil or fat is atomized in a graphite furnace, with or without a platform, connected to an atomic absorption
spectrometer which has been previously calibrated using standard solutions of an organo compound of lead.
The metal content is measured from the observed absorbance at a wavelength of 283,3 nm.
3 Reagents
Use only reagents of recognized analytical grade, unless otherwise specified, and distilled or demineralized
water or water of equivalent purity.
3.1 Cyclohexane, analytical grade.
3.2 Lecithin, containing 2 % phosphorus.
3.3 Matrix modifier: 2 % (by mass/volume) lecithin solution prepared by dissolving 2 g of lecithin (3.2) in
100 ml of cyclohexane (3.1).
3.4 Blank oil, refined.
Any edible oil is suitable, stored in a metal-free polyethylene bottle. The lead content of the oil shall not be
greater than 0,001 mg/kg.
3.5 Lead standard stock solution, c(Pb) = 10 mg/kg.
This is prepared by appropriate dilution of an organometallic standard with the blank oil (3.4).
1)
NOTE A suitable standard is available from the following companies:
 Continental Oil Company, Ponca City, Oklahoma, USA (Conostan, 5 000 mg/kg);
 Merck KGaA, Darmstadt, Germany [Certipur 115051, lead standard dissolved in oil, c(Pb) = 1 g/kg] in standard oil;
 VWR International [Certipur 1.15051, lead standard dissolved in oil, c(Pb) = 1 g/kg] in standard oil;
 VWR International [Specpure ALFA019527.AD, lead, Organic AAS standard solution, Specpure, c(Pb) = 1 000 mg/g].

1) These are examples
...