EN ISO 14403:2002

SLOVENSKI STANDARD
01-maj-2003
.DNRYRVWYRGH'RORþHYDQMHFHORWQHJDLQSURVWHJDFLDQLGD]QHSUHNLQMHQR
SUHWRþQRDQDOL]R,62
Water quality - Determination of total cyanide and free cyanide by continuous flow
analysis (ISO 14403:2002)
Wasserbeschaffenheit - Bestimmung von Gesamtcyanid und freiem Cyanid mit der
kontinuierlichen Fließanalytik (ISO 14403:2002)
Qualité de l'eau - Dosage des cyanures totaux et des cyanures libres par analyse en flux
continu (ISO 14403:2002)
Ta slovenski standard je istoveten z: EN ISO 14403:2002
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 14403
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2002
ICS 13.060.01
English version
Water quality - Determination of total cyanide and free cyanide
by continuous flow analysis (ISO 14403:2002)
Qualité de l'eau - Dosage des cyanures totaux et des Wasserbeschaffenheit - Bestimmung von Gesamtcyanid
cyanures libres par analyse en flux continu (ISO und freiem Cyanid mit der kontinuierlichen Fließanalytik
14403:2002) (ISO 14403:2002)
This European Standard was approved by CEN on 1 March 2002.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2002 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14403:2002 E
worldwide for CEN national Members.

CORRECTED  2002-04-17
Foreword
This document (ISO 14403:2002) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by September 2002, and conflicting
national standards shall be withdrawn at the latest by September 2002.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the
United Kingdom.
Endorsement notice
The text of the International Standard ISO 14403:2002 has been approved by CEN as a
European Standard without any modifications.
NOTE  Normative references to International Standards are listed in annex ZA (normative).
Annex ZA
(normative)
Normative references to International Publicationswith their relevant
European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text, and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE  Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN/HD Year
ISO 3696 1987 Water for analytical laboratory use — EN ISO 3696 1995
Specifications and test methods
INTERNATIONAL ISO
STANDARD 14403
First edition
2002-03-01
Water quality — Determination of total
cyanide and free cyanide by continuous
flow analysis
Qualité de l'eau — Dosage des cyanures totaux et des cyanures libres par
analyse en flux continu
Reference number
ISO 14403:2002(E)
©
ISO 2002
ISO 14403:2002(E)
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©
ii ISO 2002 – All rights reserved

ISO 14403:2002(E)
Contents Page
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Interferences . 2
5 Principle . 2
6 Reagents . 3
7 Apparatus . 5
8 Sampling and sample preparation . 9
9 Procedure . 10
10 Calculation . 12
11 Precision and accuracy . 13
12 Test report . 13
Annexes
A Determination of the actual cyanide concentration in potassium cyanide stock solution. 14
B Example for the determination of total cyanide and free cyanide by continuous flow analysis (CFA) with gas
diffusion and amperometric detection . 15
C Results of the interlaboratory trial for cyanide determination. 17
Bibliography. 18
©
ISO 2002 – All rights reserved iii

ISO 14403:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 14403 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee
SC 2, Physical, chemical, biochemical methods.
Annex A forms a normative part of this International Standard. Annexes B and C are for information only.
©
iv ISO 2002 – All rights reserved

ISO 14403:2002(E)
Introduction
Methods using flow analysis automate wet chemical procedures and are particularly suitable for the processing of
many analytes in water in large sample series at a high analysis frequency.
Analysis can be performed by flow injection analysis (FIA) or continuous flow analysis (CFA). In this International
Standard the latter is specified. The CFA method shares the feature of an automatic dosage of the sample into a flow
system (manifold) where the analytes in the sample react with the reagent solutions on their way through the
manifold. The sample preparation may be integrated in the manifold. The reaction product is measured in a flow
detector (e.g. flow photometer).
It is absolutely essential that the test described in this International Standard be carried out by suitable qualified staff.
It should be investigated whether and to what extend particular problems will require the specification of additional
marginal conditions.
©
ISO 2002 – All rights reserved v

INTERNATIONAL STANDARD ISO 14403:2002(E)
Water quality — Determination of total cyanide and free cyanide by
continuous flow analysis
WARNING — Persons using this International Standard should be familiar with normal laboratory practice.
This International Standard does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure
compliance with any national regulatory conditions.
1 Scope
This International Standard specifies methods for the determination of cyanide in various types of water (such as
ground, drinking, surface, leachate and waste water) with cyanide concentrations usually above 3µg/l expressed as
cyanide ions. The CFA method is applicable to a mass concentration range from 10µg/l to 100µg/l. The range of
application may be changed by varying the operation conditions.
NOTE Seawater may be analyzed with changes in sensitivity and adaptation of the reagent and calibration solutions to the
salinity of the samples.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent editions of the normative documents indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 5725-2:1994, Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method
for the determination of repeatability and reproducibility of a standard measurement method
ISO 8466-1:1990, Water quality — Calibration and evaluation of analytical methods and estimation of performance
characteristics — Part 1: Statistical evaluation of the linear calibration function
3 Terms and definitions
For the purposes of this International Standard, the following terms and definitions apply.
3.1
total cyanide
sum of some organically bound cyanides, free cyanide ions, complex compounds and cyanide bound in simple m
...