EN 17717:2024

SLOVENSKI STANDARD
oSIST prEN 17717:2023
01-maj-2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti salmonele (Salmonella spp.)
Plant biostimulants - Detection of Salmonella spp.
Pflanzen-Biostimulanzien - Nachweis von Salmonella spp.
Biostimulants des végétaux - Détection de Salmonella spp.
Ta slovenski standard je istoveten z: prEN 17717
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17717:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN 17717:2023
oSIST prEN 17717:2023
DRAFT
EUROPEAN STANDARD
prEN 17717
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2023
ICS 65.080 Will supersede CEN/TS 17717:2022
English Version
Plant biostimulants - Detection of Salmonella spp.
Biostimulants des végétaux - Détection de Pflanzen-Biostimulanzien - Nachweis von
Salmonella spp. Salmonella spp.
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17717:2023 E
worldwide for CEN national Members.

oSIST prEN 17717:2023
prEN 17717:2023 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references. 6
3 Terms and definitions . 6
4 Principle . 7
4.1 General . 7
4.2 Enrichment in selective liquid medium . 7
4.3 Plating out on selective solid media . 7
4.4 Confirmation . 7
5 Culture media, reagents, antisera . 8
5.1 General . 8
5.2 Isolation chromogenic agar . 8
5.3 Non-selective agar . 8
5.4 Confirmation selective agar . 8
6 Equipment and consumables . 8
7 Sampling . 9
8 Preparation of test sample . 9
9 Procedure . 9
9.1 Test portion and initial suspension . 9
9.2 Selective enrichment . 10
9.3 Isolation . 10
9.4 Confirmation . 10
10 Expression of results . 12
11 Performance characteristics of the method . 12
11.1 Interlaboratory studies . 12
11.2 Sensitivity . 12
11.3 Specificity . 13
11.4 Predictive positive value (PPV) . 13
11.5 Predictive negative value (NPV) . 13
12 Test report . 13
Annex A (normative) Diagram of the Procedures . 14
Annex B (normative) Culture media and reagents . 15
B.1 General . 15
B.2 Buffered peptone water (BPW) . 15
B.4 Nutrient agar (example of non-selective medium) . 16
B.5 Triple sugar iron agar (TSI agar; example for H S production agar) . 17
B.6 Saline solution . 17
oSIST prEN 17717:2023
prEN 17717:2023 (E)
B.7 Antisera . 17
B.8 Performance testing for the quality assurance of the culture media . 18
Annex C (informative) Examples of selective plating-out media . 19
Annex D (informative) Method validation study and performance characteristics . 21
D.1 Material used in the interlaboratory comparison study . 21
D.2 Interlaboratory comparison results . 21
Annex ZA (informative) Relationship of this European Standard and the essential requirements of
Regulation (EU) 2019/1009 making available on the market of EU fertilising products aimed to be
covered . 23
Bibliography . 24
oSIST prEN 17717:2023
prEN 17717:2023 (E)
European foreword
This document (prEN 17717:2023) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN enquiry.
This document will supersede CEN/TS 17717:2022.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements of EU
Directive(s) / Regulation(s).
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an integral part
of this document.
oSIST prEN 17717:2023
prEN 17717:2023 (E)
Introduction
This document was prepared by the experts of CEN/TC 455 “Plant Biostimulants”. The European
Committee for Standardization (CEN) was requested by the European Commission (EC) to draft
European standards or European standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 laying down rules on the making available on the market of
EU fertilizing products (“FPR” or “Fertilising Products Regulation”). This Standardization Request,
presented as SR M/564 and M/564Amd1, also contributes to the Communication on “Innovating for
Sustainable Growth: A Bio economy for Europe”. Working Group 5 “Labelling and denominations”, was
created to develop a work program as part of this request.
Technical committee CEN/TC 455 “Plant Biostimulants” was established to carry out the work program
that will prepare a series of standards. The interest in biostimulants has increased significantly in Europe
as a valuable tool to use in agriculture. Standardization was identified as having an important role in
order to promote the use of biostimulants. The work of CEN/TC 455 seeks to improve the reliability of
the supply chain, thereby improving the confidence of farmers, industry, and consumers in biostimulants,
and will promote and support commercialisation of the European biostimulant industry.
Biostimulants used in agriculture can be applied in multiple ways: on soil, on plants, as seed treatment,
etc. A microbial plant biostimulant consists of a microorganism or a consortium of microorganisms, as
referred to in Component Material Category 7 of Annex II of the EU Fertilizing Products Regulation (EU)
2019/1009 [1].
This document is applicable to all microbial biostimulants in agriculture.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
oSIST prEN 17717:2023
prEN 17717:2023 (E)
1 Scope
This document describes a method for the detection of Salmonella spp. in biostimulants of the following
Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products,
as described in Regulation (EU) 2019/1009 of the European Parliament and of the Council [1]:
— PFC 6(A): Microbial plant biostimulant;
— PFC 6(B): Non-microbial plant biostimulant;
— CMC 7: Microorganisms.
It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if
positive a confirmation with a serological test (and if required, a selective media).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17708:— , Plant biostimulants — Preparation of sample for microbial analysis
EN 17724:— , Plant biostimulants — Terminology
EN ISO 7218:2007 , Microbiology of food and animal feeding stuffs — General requirements and guidance
for microbiological examinations (ISO 7218:2007)
EN ISO 11133:2014 , Microbiology of food
...