ASTM D4200-82(2019)
(Test Method)Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters
Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters
SIGNIFICANCE AND USE
5.1 This test method may be applied to determine the suitability of grid-marked membrane filters for use in bacteriological culture techniques for the detection and enumeration of bacterial organisms.
5.2 A particularly sensitive organism and growth conditions have been selected for this test method in order to maximize sensitivity to toxic materials possibly present in the inks used for grid-marking membrane filters.
SCOPE
1.1 This test method describes a procedure whereby the user of ink-gridded membrane filters in water quality studies can ascertain whether or not the grid lines are toxic and inhibitory to bacterial growth when the membrane and its entrapped bacteria are incubated on a suitable media.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
- Status
- Published
- Publication Date
- 31-Oct-2019
- Technical Committee
- D19 - Water
- Drafting Committee
- D19.08 - Membranes and Ion Exchange Materials
Relations
- Effective Date
- 01-Nov-2019
- Effective Date
- 01-May-2020
- Effective Date
- 01-Mar-2010
- Effective Date
- 01-Sep-2006
- Effective Date
- 01-Sep-2006
- Effective Date
- 01-Mar-2006
- Effective Date
- 15-Feb-2006
- Effective Date
- 01-Mar-2004
- Effective Date
- 01-Mar-2004
- Effective Date
- 10-Aug-2003
- Effective Date
- 10-Mar-2003
- Effective Date
- 10-Jul-2002
- Effective Date
- 10-Jul-2002
- Effective Date
- 10-Feb-2002
- Effective Date
- 10-Feb-2002
Overview
ASTM D4200-82(2019), titled Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters, provides a precise method for determining whether the ink used to mark grids on membrane filters inhibits or affects bacterial growth. This standard is essential for laboratories and professionals involved in water quality analysis, specifically when membrane filters are used for bacterial enumeration and detection in microbiological studies. By ensuring that grid ink does not introduce toxic effects, the method helps maintain the accuracy and reliability of water microbiology results.
Key Topics
- Suitability Testing: The test method evaluates if grid-marked membrane filters are suitable for bacteriological culture by revealing any inhibitory or toxic effects from the ink used to mark the grid lines.
- Detection of Toxicity: Using a particularly sensitive bacterial organism and specific growth conditions, the method is designed to maximize detection of any toxic materials from the grid ink that may affect microbial growth.
- Qualitative Assessment: This is a qualitative test, relying on the observation of bacterial colony distribution and growth patterns near the grid lines after incubation.
- Use of Standard Procedures: The method requires standard microbiological apparatus, reagents, and procedures, ensuring reproducibility and consistency across laboratories.
- Safety and Compliance: Users are reminded to consider all safety, health, and environmental practices before conducting the test, as well as regulatory requirements.
Applications
- Water Quality Laboratories: Ensuring the membrane filters used in microbial testing do not emit inhibitory compounds from their grid markings, which could impact the detection and enumeration of bacteria such as E. coli in water samples.
- Filter Manufacturers: Quality assurance for gridded membrane filters to certify that inks used do not interfere with microbial growth, supporting product claims and regulatory compliance.
- Microbiological Research: Suitability assessment for membrane filters used in diverse research applications where accurate microbial enumeration is critical.
- Environmental Monitoring: Regulatory bodies and contract labs can implement this standard to verify the integrity of their materials for bacteriological culture techniques, ensuring valid and defensible results.
Related Standards
- ASTM D1129: Terminology Relating to Water - Provides the relevant terminology referenced in ASTM D4200-82(2019).
- ASTM D1193: Specification for Reagent Water - Describes the standard for reagent water used in the procedures outlined.
- ISO 7704: Water quality - Evaluation of membrane filters used for microbiological analysis - Offers complementary guidelines for the evaluation of membrane filter performance in water testing.
- ASTM D5465: Standard Practice for Determining Microbial Colony Counts from Membrane Filter Procedures - Related to counting techniques for membrane filter testing.
Keywords: membrane filters, ink grids, bacteriological culture, inhibitory effects, water quality, microbial testing, ASTM D4200, grid-marked filters, toxic ink, bacterial enumeration.
By adhering to ASTM D4200-82(2019), organizations can ensure that grid-marked membrane filters will not compromise microbial testing results due to toxic effects from inks. This standard supports quality assurance in environmental monitoring, water analysis, and any field where accurate detection of bacterial organisms using membrane filtration is required.
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Frequently Asked Questions
ASTM D4200-82(2019) is a standard published by ASTM International. Its full title is "Standard Test Method for Evaluating Inhibitory Effects of Ink Grids on Membrane Filters". This standard covers: SIGNIFICANCE AND USE 5.1 This test method may be applied to determine the suitability of grid-marked membrane filters for use in bacteriological culture techniques for the detection and enumeration of bacterial organisms. 5.2 A particularly sensitive organism and growth conditions have been selected for this test method in order to maximize sensitivity to toxic materials possibly present in the inks used for grid-marking membrane filters. SCOPE 1.1 This test method describes a procedure whereby the user of ink-gridded membrane filters in water quality studies can ascertain whether or not the grid lines are toxic and inhibitory to bacterial growth when the membrane and its entrapped bacteria are incubated on a suitable media. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
SIGNIFICANCE AND USE 5.1 This test method may be applied to determine the suitability of grid-marked membrane filters for use in bacteriological culture techniques for the detection and enumeration of bacterial organisms. 5.2 A particularly sensitive organism and growth conditions have been selected for this test method in order to maximize sensitivity to toxic materials possibly present in the inks used for grid-marking membrane filters. SCOPE 1.1 This test method describes a procedure whereby the user of ink-gridded membrane filters in water quality studies can ascertain whether or not the grid lines are toxic and inhibitory to bacterial growth when the membrane and its entrapped bacteria are incubated on a suitable media. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM D4200-82(2019) is classified under the following ICS (International Classification for Standards) categories: 71.040.20 - Laboratory ware and related apparatus. The ICS classification helps identify the subject area and facilitates finding related standards.
ASTM D4200-82(2019) has the following relationships with other standards: It is inter standard links to ASTM D4200-82(2011), ASTM D1129-13(2020)e2, ASTM D1129-10, ASTM D1129-06ae1, ASTM D1129-06a, ASTM D1193-06, ASTM D1129-06, ASTM D1129-04, ASTM D1129-04e1, ASTM D1129-03a, ASTM D1129-03, ASTM D1129-01, ASTM D1129-02a, ASTM D1129-02, ASTM D1129-99a. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ASTM D4200-82(2019) is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D4200 − 82 (Reapproved 2019)
Standard Test Method for
Evaluating Inhibitory Effects of Ink Grids on Membrane
Filters
This standard is issued under the fixed designation D4200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 4. Summary of Test Method
4.1 A heavy bacterial suspension is filtered through a
1.1 Thistestmethoddescribesaprocedurewherebytheuser
of ink-gridded membrane filters in water quality studies can gridded membrane filter.The bacterial concentration employed
is sufficient to cover much of the membrane with bacterial
ascertain whether or not the grid lines are toxic and inhibitory
to bacterial growth when the membrane and its entrapped colonies.
bacteria are incubated on a suitable media.
4.2 After filtration the membrane is incubated on a suitable
medium and the distribution of the colonies and shape of the
1.2 The values stated in SI units are to be regarded as
colonies noted in the area around each grid line.
standard. No other units of measurement are included in this
standard.
5. Significance and Use
1.3 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the 5.1 This test method may be applied to determine the
responsibility of the user of this standard to establish appro- suitability of grid-marked membrane filters for use in bacte-
priate safety, health, and environmental practices and deter- riological culture techniques for the detection and enumeration
mine the applicability of regulatory limitations prior to use. of bacterial organisms.
1.4 This international standard was developed in accor-
5.2 Aparticularly sensitive organism and growth conditions
dance with internationally recognized principles on standard-
have been selected for this test method in order to maximize
ization established in the Decision on Principles for the
sensitivity to toxic materials possibly present in the inks used
Development of International Standards, Guides and Recom-
for grid-marking membrane filters.
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
6. Apparatus
6.1 Incubator, capable of maintaining temperatures of
2. Referenced Documents
44.5 6 0.2°C.
2.1 ASTM Standards:
6.2 Membrane Filtration Units.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
6.3 Vacuum Source with trap vessel.
6.4 Forceps, blunt-nosed.
3. Terminology
6.5 Autoclave or other sterilizing equipment.
3.1 Definitions:
6.6 Expendables:
3.1.1 For definitions of terms used in this standard, refer to
6.6.1 Gridded membrane filters.
Terminology D1129.
6.6.2 1-mL and 10-mL pipets.
6.6.3 Petri dishes (50-mm) containing 6 to 8 mL of agar
medium or a 100-mm dish with 20 6 2 mLof agar medium, or
This test method is under the jurisdiction of ASTM Committee D19 on Water
both.
and is the direct responsibility of Subcommittee D19.08 on Membranes and Ion
Exchange Materials. 6.6.4 Erlenmeyer flasks.
Current edition approved Nov. 1, 2019. Published January 2020. Originally
approved in 1982. Last previous edition approved in 2011 as D4200 – 82 (2011).
7. Reagents and Materials
DOI: 10.1520/D4200-82R19.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
7.1 Purity of Water—Unless otherwise indicated, reference
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
to water shall be understood to mean reagent water confor
...
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