ASTM E1047-85(2021)
(Specification)Standard Specification for Blood Sedimentation Tube, Wintrobe, Glass, Disposable
Standard Specification for Blood Sedimentation Tube, Wintrobe, Glass, Disposable
ABSTRACT
This specification covers disposable blood sedimentation tubes suitable for determining sedimentation rates and the volume of packed red blood cells. This specification covers a tube intended for one-time use only. The tubes shall be fabricated borosilicate glass, Type I, Class B, or from soda lime glass, Type II. The tubes shall be tested for their marking permanency and resistance to centrifugal test.
SCOPE
1.1 This specification covers disposable blood sedimentation tubes suitable for determining sedimentation rates and the volume of packed red blood cells.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 The following precautionary statement pertains only to the test method portion, Section 7 of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
- Status
- Published
- Publication Date
- 31-Dec-2020
- Technical Committee
- E41 - Laboratory Apparatus
- Drafting Committee
- E41.01 - Laboratory Ware and Supplies
Relations
- Effective Date
- 01-Feb-2024
- Effective Date
- 01-Jan-2018
- Effective Date
- 01-Dec-2011
- Effective Date
- 01-Nov-2006
- Effective Date
- 15-Aug-1993
- Effective Date
- 15-Aug-1993
Overview
ASTM E1047-85(2021) - Standard Specification for Blood Sedimentation Tube, Wintrobe, Glass, Disposable establishes the requirements for disposable Wintrobe blood sedimentation tubes made from glass. These laboratory tubes are designed specifically for one-time use in determining blood sedimentation rates and measuring the volume of packed red blood cells. The standard specifies the construction, material composition, physical dimensions, and applicable test methods to ensure accuracy and safety in laboratory applications.
This specification is accredited and maintained by ASTM International, following internationally recognized standardization principles outlined by the World Trade Organization TBT Committee.
Key Topics
- Materials: Tubes must be made from borosilicate glass, Type I, Class B, or soda lime glass, Type II, ensuring chemical resistance and clarity. Materials must adhere to ASTM E438 for laboratory glassware.
- Design and Dimensions:
- Minimum wall thickness of 0.6 mm
- Uniform cylindrical body with a round outside bottom and flat inside bottom
- Outside diameter: 5.0 ± 0.5 mm; inside diameter: 2.9 to 3.5 mm
- Length: 110 to 117 mm
- Graduated scale: 100 to 105 mm in 1 mm divisions, numbered each centimeter on both sides
- Markings: Clear, legible graduations and numerals are required. A wide color band at the top is included for identification. Markings must withstand normal use as verified by marking permanency tests.
- Performance Testing:
- Marking permanency: Ensures inscriptions and graduation lines remain legible during routine handling.
- Centrifugal resistance: Tubes must withstand at least 2500 g in a centrifuge without chipping, cracking, or breaking.
- Single-use Specification: Defined explicitly for one-time use to prevent cross-contamination and ensure reliability in clinical and research settings.
Applications
Wintrobe glass blood sedimentation tubes are essential in clinical laboratories for hematology testing, specifically for:
- Erythrocyte Sedimentation Rate (ESR): Accurate measurement of sedimentation rates to help in diagnosing and monitoring various medical conditions such as infections, inflammatory diseases, and certain blood disorders.
- Packed Red Blood Cell Volume Measurement: Determining the precise volume of erythrocytes in a blood sample, which is crucial for assessing patient health and guiding treatment decisions.
- Research Laboratories: Used in research studies involving blood sedimentation and hematological analysis.
These disposable tubes promote laboratory efficiency by reducing the risk of contamination, ensuring uniformity, and streamlining sample processing and disposal.
Related Standards
The ASTM E1047-85(2021) standard references and aligns with the following related standards to support the consistent, safe, and effective use of Wintrobe blood sedimentation tubes:
- ASTM E438 - Standard Specification for Glasses in Laboratory Apparatus: Specifies the composition, properties, and performance criteria for glassware used in scientific applications.
- Other Hematology Tube Standards: There are various other standards for reusable and disposable blood sedimentation tubes, which may differ in materials, tolerances, and cleaning protocols.
Practical Value
Implementing ASTM E1047-85(2021) ensures laboratories use safe, reliable, and well-calibrated disposable glass tubes for sedimentation and volumetric analysis. Compliance with this internationally recognized standard supports valid results, reduces contamination risks, and promotes best practices in clinical and research hematology.
Keywords: ASTM E1047, Wintrobe tube, blood sedimentation tube, disposable glass tube, laboratory standards, hematology, ESR, borosilicate glass, soda lime glass, clinical laboratory supplies.
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Frequently Asked Questions
ASTM E1047-85(2021) is a technical specification published by ASTM International. Its full title is "Standard Specification for Blood Sedimentation Tube, Wintrobe, Glass, Disposable". This standard covers: ABSTRACT This specification covers disposable blood sedimentation tubes suitable for determining sedimentation rates and the volume of packed red blood cells. This specification covers a tube intended for one-time use only. The tubes shall be fabricated borosilicate glass, Type I, Class B, or from soda lime glass, Type II. The tubes shall be tested for their marking permanency and resistance to centrifugal test. SCOPE 1.1 This specification covers disposable blood sedimentation tubes suitable for determining sedimentation rates and the volume of packed red blood cells. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 The following precautionary statement pertains only to the test method portion, Section 7 of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ABSTRACT This specification covers disposable blood sedimentation tubes suitable for determining sedimentation rates and the volume of packed red blood cells. This specification covers a tube intended for one-time use only. The tubes shall be fabricated borosilicate glass, Type I, Class B, or from soda lime glass, Type II. The tubes shall be tested for their marking permanency and resistance to centrifugal test. SCOPE 1.1 This specification covers disposable blood sedimentation tubes suitable for determining sedimentation rates and the volume of packed red blood cells. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 The following precautionary statement pertains only to the test method portion, Section 7 of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM E1047-85(2021) is classified under the following ICS (International Classification for Standards) categories: 11.100.30 - Analysis of blood and urine. The ICS classification helps identify the subject area and facilitates finding related standards.
ASTM E1047-85(2021) has the following relationships with other standards: It is inter standard links to ASTM E438-92(2024), ASTM E438-92(2018), ASTM E438-92(2011), ASTM E438-92(2006), ASTM E438-92(2001)e1, ASTM E438-92(1996). Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ASTM E1047-85(2021) is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E1047 −85 (Reapproved 2021)
Standard Specification for
Blood Sedimentation Tube, Wintrobe, Glass, Disposable
This standard is issued under the fixed designation E1047; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.2 Wintrobe , n—surname of the individual responsible
for the design of the reusableWintrobe tube and the method of
1.1 This specification covers disposable blood sedimenta-
use for both reusable and disposable tubes.
tion tubes suitable for determining sedimentation rates and the
volume of packed red blood cells.
4. Classification
1.2 The values stated in SI units are to be regarded as
4.1 This specification covers a tube that is intended to be
standard. No other units of measurement are included in this
usedonetimeonly.Itisnottobeconfusedwithareusabletube
standard.
that is described in other published standards.
1.3 The following precautionary statement pertains only to
the test method portion, Section 7 of this specification. This 5. Materials
standard does not purport to address all of the safety concerns,
5.1 Glass—The tubes made to this specification shall be
if any, associated with its use. It is the responsibility of the user
fabricatedborosilicateglass,TypeI,ClassB,orfromsodalime
of this standard to establish appropriate safety, health, and
glass, Type II, in accordance with Specification E438.
environmental practices and determine the applicability of
regulatory limitations prior to use.
6. Physical Requirements
1.4 This international standard was developed in accor-
6.1 Design—The tube shall be made of tubing with a
dance with internationally recognized principles on standard-
minimum wall thickness of 0.6 mm. The tube shall have a
ization established in the Decision on Principles for the
cylindrical body terminating in a round bottom on the outside
Development of International Standards, Guides and Recom-
and a flat bottom on the inside. The tube shall have a uniform
mendations issued by the World Trade Organization Technical
bore and a fire-polished top.
Barriers to Trade (TBT) Committee.
6.2 Workmanship—The tube shall be free as possible from
visible defects which detract from its serviceability. The tube
2. Referenced Documents
shall be well annealed so there will be no ring strain evident
2.1 ASTM Standards:
when tested under a polariscope.
E438Specification for Glasses in Laboratory Apparatus
6.3 Dimensions—The tube shall be made of tubing with an
outside diameter (O.D.) of 5.0 6 0.5 mm and an inside
3. Terminology
diameter (I.D.) of 2.9 to 3.5 mm. The uniformity of the bore
3.1 Definitions of Terms Specific to This Standard:
shall be 60.15 mm throughout the tube. The tube shall be 110
3.1.1 disposable, n—in accordance with this specification
to 117 mm long and have a graduated scale of 100 mm.
and the expected product performance expressed in this
Manufacturers have the option to place additional graduation
standard, those tubes which are to be used one time only.Any
lines on the tube to a maximum of 105 mm. Maximum overall
institutionorindividualwhoreusesadisposabletubemustbear
tolerance on the total graduation scale shall be 61.0 mm. The
full responsibility for its safety and effectiveness.
tube shall be legibly marked with the maker’s or vendor’s
name or mark and possess a wide color band at the top of the
tube for marking purposes.
This specification is under the jurisdiction of ASTM Committee E41 on
6.4 Graduation Scale—The tube shall be graduated 100 to
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
105 6 1.0 mm in 1 mm divisions and numbered every
Laboratory Ware and Supplies.
Current edition approved Jan. 1, 2021. Published February 2021. Originally
centimetre with two sets of numerals. One set of graduation
ap
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