ASTM E723-07

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Designation: E723 − 07
StandardTest Method for
Efficacy of Antimicrobials as Preservatives for Aqueous-
Based Products Used in the Paper Industry (Bacterial
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Spoilage)
This standard is issued under the fixed designation E723; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.3 preservatives, n—a chemical agent(s) added to a
product to reduce or prevent microbial growth.
1.1 This laboratory test method is used to determine the
efficacyofanantimicrobialforpreventingbacterialspoilageof
4. Summary of Test Method
in-process aqueous-based products used in the paper industry.
4.1 Aqueous material to be preserved is inoculated with an
For information on fungal spoilage, see Test Method E875.
appropriate bacterial inoculum followed by addition of a
This test method should be performed by persons who have
bactericidethatwillreducepopulationsofbacteriaandprevent
had basic microbiological training.
thegrowthofsurvivorsforaspecifiedperiodoftime.Bacterial
1.2 This standard does not purport to address all of the
numbers in the sample are determined at various time periods
safety concerns, if any, associated with its use. It is the
and compared to a contol without any bactericide. The proper
responsibility of the user of this standard to establish appro-
level of antimicrobial is one that reduces and keeps the
priate safety and health practices and determine the applica-
organisms to an acceptable level in the test material.
bility of regulatory limitations prior to use.
5. Significance and Use
2. Referenced Documents
5.1 This test method should be used to determine if an
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2.1 ASTM Standards:
antimicrobial preserves pigment suspensions, dye solutions,
D1193Specification for Reagent Water
pulp slurries, starch solutions, polymers, sizing agents, latex
E875Test Method for Efficacy of Fungal ControlAgents as
emulsions,andotheraqueous-basedmaterialsusedinthepaper
Preservatives for Aqueous-Based Products Used in the
industry from bacterial spoilage.
Paper Industry
E1054Test Methods for Evaluation of Inactivators of Anti-
6. Apparatus
microbial Agents
6.1 Balance—Two balances: one should be sensitive to 0.1
E1326GuideforEvaluatingNonconventionalMicrobiologi-
g at a load of 200 g and have a platform to accommodate
cal Tests Used for Enumerating Bacteria
bottles being used in the test. The second balance (analytical)
shouldbesensitiveto0.1mgandshouldbeemployedtoweigh
3. Terminology
the candidate preservative to be used in the preparation of the
3.1 Definitions of Terms Specific to This Standard:
stock solutions.
3.1.1 antimicrobial, n—chemicalorphysicalagentthatkills
6.2 Bottles—Borosilicateglassmilkdilutionbottlesorother
or inactivates microorganisms or suppresses their growth or
suitable containers fitted either with screw caps or Escher
reproduction.
rubber stoppers. These bottles are used for water blanks and
3.1.2 bactericide, n—a physical or chemical agent that kills
aqueous-based samples.
bacteria, but not necessarily bacterial spores.
6.3 Colony Counter—Anyoneofseveraltypesmaybeused
as the Quebec, Buck, and Wolfhuegel. A hand tally for the
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This test method is under the jurisdiction of ASTM Committee E35 on
recording of the bacterial count is recommended if manual
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
counting is done. Alternatively, an automated video colony
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2007. Published April 2007. Originally counter may also be used.
approved in 1980. Last previous edition approved in 2002 as E723–97 (2002).
6.4 Culture Tube Closures—Appropriate nontoxic closures
DOI: 10.1520/E0723-07.
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or should be selected.
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
6.5 Culture Tubes—Recommended size is 15 by 125 mm or
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. 18by150mmwithoutlip,andpreferablyofborosilicateglass.
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E723 − 07
6.6 Blender—Any blender that will assure proper agitation 8.3 The material under test should be inoculated with
and blending. sufficient microorganisms either from pure cultures or con-
tained in the spoiled material used as the inoculum to give a
6.7 Flaming Equipment—Depending upon circumstances,
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bacterial count of 1×10 CFU/mL or higher.
either an alco
...