ASTM E714-94(2021)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation:E714 −94 (Reapproved 2021)
Standard Specification for
Disposable Glass Serological Pipets
This standard is issued under the fixed designation E714; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope time only. Any institution or individual who reuses a dispos-
able pipet must bear full responsibility for its safety and
1.1 This specification covers disposable glass serological
effectiveness.
pipets, calibrated “to deliver,” used in measuring volumes of
liquids.
4. Materials and Manufacture
1.2 This international standard was developed in accor-
4.1 The pipets shall be fabricated from borosilicate glass,
dance with internationally recognized principles on standard-
Type I, ClassAor B; or soda-lime glass, Type II, as defined in
ization established in the Decision on Principles for the
Specification E438.
Development of International Standards, Guides and Recom-
mendations issued by the World Trade Organization Technical 5. Design
Barriers to Trade (TBT) Committee.
5.1 Shape—Pipets shall be straight and of one-piece con-
struction. Any cross section of a pipet taken in a plane
2. Referenced Documents
perpendicular to the longitudinal axis shall be circular.
2.1 ASTM Standards:
5.2 Delivery Tips—Delivery tips shall be made with a
E438 Specification for Glasses in Laboratory Apparatus
gradual taper of 10 to 25 mm for capacities up to 2 cm
E542 Practice for Calibration of Laboratory Volumetric
3 3
inclusive, and 15 to 30 mm for the 5 cm and 10 cm size(s).
Apparatus
The tip end shall be reasonably perpendicular to the longitu-
E920 Specification for Commercially Packaged Laboratory
dinal axis of the pipet and shall be firepolished.
Apparatus
3 3
5.3 Top End—The 10 cm and 25 cm pipet shall have a top
E921 Specification for Export Packaged Laboratory Appa-
end tooled to a diameter of 7 to 9 mm and shall have an overall
ratus
length of 15 to 25 mm from the top. The top end shall be
E1133 Practice for Performance Testing of Packaged Labo-
suitable for plugging with filtering material. All top ends shall
ratory Apparatus for United States Government Procure-
be reasonably perpendicular to the longitudinal axis of the
ments
pipet, and shall be firepolished.
3. Terminology
5.4 Dimensions and Outflow Times—The limiting dimen-
3.1 Definitions of Terms Specific to This Standard: sions and outflow times shall be as shown in Table 1. Outflow
3.1.1 accuracy—the expected distribution of mean volumes times shall be determined on unplugged pipets, using distilled
around the stated volume. water at 25 6 5 °C, and by means of a stopwatch.
3.1.2 coeffıcient of variation—the expected distribution of
6. Markings
individual volumes around the mean volume.
6.1 Graduation Markings—Graduation lines shall be be-
3.1.3 disposable pipets—in accordance with this specifica-
tween 0.2 and 0.5 mm in width, and in a plane perpendicular to
tion and the expected product performance expressed in this
the longitudinal axis of the pipet and parallel to each other. A
specification, those serological pipets which are to be used one
maingraduationlineshallextendatleastthree-fifthsoftheway
around the pipet. The values of all main graduation lines shall
be in Arabic numerals directly above the line referenced.
This specification is under the jurisdiction of ASTM Committee E41 on
Intermediate graduation lines shall extend at least one-fifth of
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
Laboratory Ware and Supplies.
the way around the pipet, and least graduation lines shall
Current edition approved Jan. 1, 2021. Published February 2021. Originally
extend at least one-seventh of the distance around the pipet.
approvedin1980.Lastpreviouseditionapprovedin2015asE714 – 94(2015).DOI:
The 0 graduation line shall be at least 90 mm below the top of
10.1520/E0714-94R21.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or the pipet on all sizes.
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