SIST EN 13368-2:2018
(Main)Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
This European Standard specifies a method for the chromatographic determination of the iron chelated by each individual ortho(hydroxy)-ortho(hydroxy) isomer of the chelating agents [o,o] EDDHA, [o,o] EDDHMA and by HBED in fertilizers containing one or more of these substances, except for [o,o] EDDHMA and HBED mixes. The method allows the identification and the determination of the total concentration of water soluble iron chelates of these chelating agents. Also, after derivatization with Fe, the soluble amount of the chelating agents can be determined when other micro-nutrients, beside Fe are present in fertilizers containing [o,o] EDDHA, [o,o] EDDHMA or HBED.
This method is applicable to EC fertilizers covered by Regulation (EC) No 2003/2003 [4]. It is applicable to a mass fraction of the metal chelated of at least 0,625 %.
NOTE 1 The substances EDDHA (ethylenediamine-N,N'-di[(hydroxyphenyl)acetic acid] and EDDHMA (ethylenediamine-N,N'-di[(hydroxymethylphenyl)acetic acid] exist as several different isomeric forms. Positional isomers for the hydroxyl or methyl groups (in ortho, meta, and para positions) as well as stereo isomers (meso and dl-racemic forms) are known. Both meso and dl-racemic forms of the [ortho,ortho] EDDHA and [ortho,ortho] EDDHMA are positional isomers for the hydroxyl groups allowed by the Regulation (EC) No 2003/2003. Since para, meta and ortho methyl positional isomers of the EDDHMA present quite similar stability, they could be grouped: in the method here described the para, meta and ortho methyl positional isomers of the [o,o] EDDHMA are considered together. HBED (N,N'-bis(2-hydroxybenzyl)-ethylenediamine-N,N'-diacetic acid) does not present isomeric forms.
NOTE 2 At present, analytically pure standards only exist for [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA and HBED. All other substances being unavailable as a standard, the influence of their eventual presence in the samples (with respect to the sensitivity and the selectivity of this method) has not been studied.
NOTE 3 The meso and the dl-racemic forms of [o,o] EDDHA and [o,o] EDDHMA can be determined separately by this method.
Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 2: Bestimmung von Fe chelatisiertem [o,o] EDDHA, [o,o] EDDHMA und HBED, oder der Menge der Chelatbildner, mit Ionen-Paarchromatographie
Diese Europäische Norm legt ein Verfahren zur chromatographischen Bestimmung von Eisen, das mit jedem individuellen ortho(hydroxy)-ortho(hydroxy)-Isomer der Chelatbildner [o,o] EDDHA, [o,o] EDDHMA und mit HBED Chelate bildet, in Düngemitteln fest, die eine oder mehrere dieser Substanzen enthalten, mit Ausnahme von [o,o] EDDHMA und HBED-Gemischen. Das Verfahren erlaubt die Identifizierung und die Bestimmung der Gesamtkonzentration von wasserlöslichen Eisenchelaten dieser Chelatbildner. Nach der Derivatisierung mit Fe ist auch die Bestimmung des löslichen Anteils der Chelatbildner möglich, wenn andere Spurennährstoffe außer Fe in Düngemitteln vorhanden sind, die [o,o] EDDHA, [o,o] EDDHMA oder HBED enthalten.
Dieses Verfahren ist anwendbar für EG-Düngemittel, die der Verordnung (EG) Nr. 2003/2003 [4] unterliegen. Es gilt für einen Massenanteil an chelatisiertem Metall von mindestens 0,625 %.
ANMERKUNG 1 Die Substanzen EDDHA (Ethylendiamin-N,N'-di[(hydroxyphenyl)essigsäure)] und EDDHMA (Ethylendiamin-N,N'-di[(hydroxy-methylphenyl)essigsäure)] existieren in mehreren unterschiedlichen isomeren Formen. Es sind sowohl Stellungsisomere (in Ortho-, Meta- und Para-Stellungen) als auch Stereoisomere (Meso- und dl-Racemformen) der Hydroxyl- oder Methylgruppen bekannt. Die Meso- und dl-Racemformen von ortho,ortho-EDDHA und ortho,ortho-EDDHMA sind Stellungsisomere der Hydroxylgruppen, die nach der Verordnung (EG) Nr. 2003/2003 zugelassen sind. Da Para-, Meta- und Orthomethyl-Stellungsisomere von EDDHMA eine sehr ähnliche Stabilität aufweisen, könnten sie in einer Gruppe zusammengefasst werden: in dem hier beschriebenen Verfahren werden die Para-, Meta- und Orthomethyl-Stellungsisomere von [o,o] EDDHMA zusammen betrachtet. HBED (N,N'-Bis(2-hydroxybenzyl)ethylendiamin-N,N'-diessigsäure) bildet keine isomeren Formen aus.
ANMERKUNG 2 Gegenwärtig existieren analysenreine Standards nur für ortho,ortho-EDDHA, ortho,ortho-EDDHMA und HBED. Da alle anderen Substanzen als Standards nicht zur Verfügung stehen, ist deren Einfluss bei eventuellem Vorkommen in den Proben (in Bezug auf die Empfindlichkeit und die Trennschärfe dieses Verfahrens) noch nicht untersucht worden.
ANMERKUNG 3 Mit diesem Verfahren können die Meso- und dl-Racemformen von [o,o] EDDHA und [o,o] EDDHMA einzeln bestimmt werden.
Engrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 2: Détermination du fer chélaté [o,o] EDDHA, [o,o] EDDHMA et HBED, ou de la quantité d'agents chélatants, par chromatographie d'appariement d'ions
La présente norme européenne spécifie une méthode de détermination par chromatographie du fer chélaté par chacun des isomères ortho(hydroxy)-ortho(hydroxy) des agents chélatants [o,o] EDDHA et [o,o] EDDHMA et par l'HBED dans les engrais contenant l'une ou plusieurs de ces substances, à l'exception des mélanges de [o,o] EDDHMA et d'HBED. La méthode permet l'identification et la détermination de la concentration totale des chélates de fer solubles dans l’eau de ces agents chélatants. Par ailleurs, après dérivatisation avec le fer, la quantité d’agents chélatants soluble peut être déterminée, quand d’autres oligo-éléments que le fer sont présents, dans les engrais contenant [o,o] EDDHA, [o,o] EDDHMA ou HBED.
La méthode décrite concerne les engrais européens couverts par le Règlement (CE) n°2003/2003 [4]. Il est applicable à une teneur en métal chélaté d'au moins 0,625 %.
NOTE 1 Les substances EDDHA (acide éthylènediamine-N,N'-di[(hydroxyphényl)acétique] et EDDHMA (acide éthylènediamine-N,N'-di(hydroxy-méthylphényl)acétique] existent sous plusieurs formes isomères différentes. Des isomères de position des groupes hydroxyl et méthyl (en position ortho, méta et para) sont connus, aussi bien que des stéréo-isomères (formes méso et dl-racémiques). Les deux formes méso et dl-racémiques de [ortho,ortho] EDDHA et de [ortho,ortho] EDDHMA sont des isomères de position des groupes hydroxyl autorisés par la réglementation (CE) n° 2003/2003. Dans la mesure où les isomères de position ortho, méta et para des groupes méthyl de l'EDDHMA présentent une stabilité tout à fait similaire, ils ont pu être regroupés. Dans la méthode décrite ici, les isomères de position ortho, méta et para des groupes méthyl de l'[o,o]-EDDHMA sont donc considérés conjointement. L'HBED (acide N,N´-bis(2-hydroxybenzyl)-éthylènediamine-N,N´-diacétique) ne présente pas de formes isomères.
NOTE 2 À l'heure actuelle, il n'existe de produit de référence pur pour analyse que pour [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA et l'HBED. Aucune autre substance n'étant disponible en tant que produit de référence, l'influence de leur présence éventuelle dans les échantillons n'a fait l'objet d'aucune étude (du point de vue de la sensibilité et de la sélectivité de la méthode).
NOTE 3 Les formes méso et dl-racémiques de [o,o] EDDHA et [o,o] EDDHMA peuvent être déterminées séparément par la présente méthode.
Gnojila - Določevanje sredstev za kelatiziranje v gnojilih s kromatografijo - 2. del: Določevanje železovih kelatov z [o,o] EDDHA, [o,o] EDDHMA in HBED ali količine sredstev za kelatiziranje s kromatografijo ionskih parov
Ta evropski standard določa metodo za kromatografsko določevanje železovih kelatov z vsakim posameznim orto(hidroksi)-orto(hidroksi) izomerom kelatov [o,o] EDDHA, [o,o] EDDHMA in HBED v gnojilih, ki vsebujejo eno ali več teh snovi, razen mešanic [o,o] EDDHMA in HBED. Metoda omogoča opredelitev in določevanje skupne koncentracije v vodi topnih železovih kelatov teh kelatov. Po derivatizaciji z železom se lahko količina topnih kelatov določi tudi, ko so v gnojilih, ki vsebujejo kelate [o,o] EDDHA, [o,o] EDDHMA ali HBED, prisotna druga mikrohranila razen železa.
Ta metoda se uporablja za gnojila ES, navedena v Uredbi (ES) št. 2003/2003 [4]. Uporablja se za masni delež kovinskih kelatov najmanj 0,625 %.
OPOMBA 1: Snovi EDDHA (etilendiamin-N,N'-di[(hidroksifenil)ocetna kislina] in EDDHMA (etilendiamin-N,N'-di[(hidroksimetilfenil)ocetna kislina] obstajajo v različnih izomernih oblikah. Znani so pozicijski izomeri hidroksilnih ali metilnih skupin (na pozicijah orto, meta in para) in stereo izomeri (oblike mezo in dl-racemične oblike). Oblike mezo ter dl-racemične oblike [orto,orto] EDDHA in [orto,orto] EDDHMA so pozicijski izomeri hidroksilnih skupin, ki jih dovoljuje Uredba (ES) št. 2003/2003. Ker je stabilnost para-, meta- in ortometil pozicijskih izomerov EDDHMA podobna, jih je mogoče razvrstiti v isto skupino: v metodi, opisani v tem dokumentu, so para-, meta- in ortometil pozicijski izomeri [o,o] EDDHMA obravnavani skupaj. HBED (N,N'-bis(2-hidroksibenzil)-etilendiamin-N,N'-diocetna kislina) nima izomernih oblik.
OPOMBA 2: Analitično neoporečni standardi trenutno obstajajo samo za [orto,orto] EDDHA, [orto,orto] EDDHMA in HBED. Ker druge snovi niso standardizirane, vpliv njihove možne prisotnosti v vzorcih (glede na občutljivost in primernost te metode) ni bil preučen.
OPOMBA 3: Oblike mezo in dl-racemične oblike [o,o] EDDHA in [o,o] EDDHMA je s to metodo mogoče določiti ločeno.
General Information
- Status
- Published
- Public Enquiry End Date
- 01-Aug-2016
- Publication Date
- 07-Dec-2017
- Technical Committee
- KAT - Soil quality
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 17-Oct-2017
- Due Date
- 22-Dec-2017
- Completion Date
- 08-Dec-2017
Relations
- Effective Date
- 01-Jan-2018
Overview
EN 13368-2:2017 (CEN) defines a validated chromatographic method for the determination of iron chelates and related chelating agents in fertilizers. The standard covers the isocratic ion‑pair high‑performance liquid chromatography (HPLC) separation and photometric detection of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, and describes how, after Fe‑derivatization, the soluble amounts of these chelating agents can be quantified even when other micronutrients are present. The method is applicable to EC fertilizers under Regulation (EC) No 2003/2003, for samples where the mass fraction of metal chelated is ≥ 0.625 %.
Key topics
- Analytical scope
- Identification and quantification of water‑soluble Fe‑chelates of [o,o] EDDHA, [o,o] EDDHMA and HBED.
- Determination of the chelating agent (after Fe derivatization) when other micro‑nutrients are present.
- Not applicable to certain mixes (see standard text for exclusions).
- Method principle
- Ion‑pair chromatography on a reverse‑phase silica column with tetrabutylammonium (TBA+) and acetonitrile eluent.
- Photometric detection (280 nm) to resolve individual ortho‑ortho isomers; meso and dl‑racemic forms can be separated.
- Performance and limitations
- Precision and reproducibility supported by inter‑laboratory tests (annexed results).
- Pure analytical standards are currently available only for [o,o] EDDHA, [o,o] EDDHMA and HBED; effects of other isomers not fully studied.
- Potential peak overlap (e.g., meso [o,o] EDDHMA vs HBED) can occur depending on column - column selection may be required.
Applications
Who uses EN 13368-2:2017 and why:
- Fertilizer manufacturers - quality control of micronutrient formulations containing Fe‑chelates to ensure label accuracy and regulatory compliance.
- Analytical laboratories - routine testing and method validation for iron chelate content in water‑soluble fertilizer products.
- Regulatory authorities - verification of compliance with EC fertilizer regulations and permitted chelating agent declarations.
- R&D teams - formulation development and stability testing of chelated micronutrient products.
Practical benefits:
- Enables precise determination of chelated iron (Fe‑chelates) and, after derivatization, the chelator content even in complex fertilizer matrices.
- Supports product registration, performance specification, and traceability of micronutrient content.
Related standards
- EN 13368 series:
- Part 1 - EDTA, HEEDTA and DTPA by ion chromatography
- Part 3 - [S,S]-EDDS by ion pair chromatography
- EN 1482-2 - Sampling and sample preparation for fertilizers
- EN ISO 3696 - Water for analytical laboratory use
- Regulation (EC) No 2003/2003 - EC fertilizers
Keywords: EN 13368-2:2017, ion pair chromatography, iron chelates, [o,o] EDDHA, [o,o] EDDHMA, HBED, fertilizers testing, HPLC, chelating agents, EC fertilizers.
Frequently Asked Questions
SIST EN 13368-2:2018 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Fertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography". This standard covers: This European Standard specifies a method for the chromatographic determination of the iron chelated by each individual ortho(hydroxy)-ortho(hydroxy) isomer of the chelating agents [o,o] EDDHA, [o,o] EDDHMA and by HBED in fertilizers containing one or more of these substances, except for [o,o] EDDHMA and HBED mixes. The method allows the identification and the determination of the total concentration of water soluble iron chelates of these chelating agents. Also, after derivatization with Fe, the soluble amount of the chelating agents can be determined when other micro-nutrients, beside Fe are present in fertilizers containing [o,o] EDDHA, [o,o] EDDHMA or HBED. This method is applicable to EC fertilizers covered by Regulation (EC) No 2003/2003 [4]. It is applicable to a mass fraction of the metal chelated of at least 0,625 %. NOTE 1 The substances EDDHA (ethylenediamine-N,N'-di[(hydroxyphenyl)acetic acid] and EDDHMA (ethylenediamine-N,N'-di[(hydroxymethylphenyl)acetic acid] exist as several different isomeric forms. Positional isomers for the hydroxyl or methyl groups (in ortho, meta, and para positions) as well as stereo isomers (meso and dl-racemic forms) are known. Both meso and dl-racemic forms of the [ortho,ortho] EDDHA and [ortho,ortho] EDDHMA are positional isomers for the hydroxyl groups allowed by the Regulation (EC) No 2003/2003. Since para, meta and ortho methyl positional isomers of the EDDHMA present quite similar stability, they could be grouped: in the method here described the para, meta and ortho methyl positional isomers of the [o,o] EDDHMA are considered together. HBED (N,N'-bis(2-hydroxybenzyl)-ethylenediamine-N,N'-diacetic acid) does not present isomeric forms. NOTE 2 At present, analytically pure standards only exist for [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA and HBED. All other substances being unavailable as a standard, the influence of their eventual presence in the samples (with respect to the sensitivity and the selectivity of this method) has not been studied. NOTE 3 The meso and the dl-racemic forms of [o,o] EDDHA and [o,o] EDDHMA can be determined separately by this method.
This European Standard specifies a method for the chromatographic determination of the iron chelated by each individual ortho(hydroxy)-ortho(hydroxy) isomer of the chelating agents [o,o] EDDHA, [o,o] EDDHMA and by HBED in fertilizers containing one or more of these substances, except for [o,o] EDDHMA and HBED mixes. The method allows the identification and the determination of the total concentration of water soluble iron chelates of these chelating agents. Also, after derivatization with Fe, the soluble amount of the chelating agents can be determined when other micro-nutrients, beside Fe are present in fertilizers containing [o,o] EDDHA, [o,o] EDDHMA or HBED. This method is applicable to EC fertilizers covered by Regulation (EC) No 2003/2003 [4]. It is applicable to a mass fraction of the metal chelated of at least 0,625 %. NOTE 1 The substances EDDHA (ethylenediamine-N,N'-di[(hydroxyphenyl)acetic acid] and EDDHMA (ethylenediamine-N,N'-di[(hydroxymethylphenyl)acetic acid] exist as several different isomeric forms. Positional isomers for the hydroxyl or methyl groups (in ortho, meta, and para positions) as well as stereo isomers (meso and dl-racemic forms) are known. Both meso and dl-racemic forms of the [ortho,ortho] EDDHA and [ortho,ortho] EDDHMA are positional isomers for the hydroxyl groups allowed by the Regulation (EC) No 2003/2003. Since para, meta and ortho methyl positional isomers of the EDDHMA present quite similar stability, they could be grouped: in the method here described the para, meta and ortho methyl positional isomers of the [o,o] EDDHMA are considered together. HBED (N,N'-bis(2-hydroxybenzyl)-ethylenediamine-N,N'-diacetic acid) does not present isomeric forms. NOTE 2 At present, analytically pure standards only exist for [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA and HBED. All other substances being unavailable as a standard, the influence of their eventual presence in the samples (with respect to the sensitivity and the selectivity of this method) has not been studied. NOTE 3 The meso and the dl-racemic forms of [o,o] EDDHA and [o,o] EDDHMA can be determined separately by this method.
SIST EN 13368-2:2018 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers; 71.040.50 - Physicochemical methods of analysis. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN 13368-2:2018 has the following relationships with other standards: It is inter standard links to SIST EN 13368-2:2013. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN 13368-2:2018 is associated with the following European legislation: EU Directives/Regulations: 2003/2003, 2019/1009. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
You can purchase SIST EN 13368-2:2018 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie - Teil 2: Bestimmung von Fe chelatisiertem [o,o] EDDHA, [o,o] EDDHMA und HBED, oder der Menge der Chelatbildner, mit Ionen-PaarchromatographieEngrais - Détermination des agents chélatants dans les engrais par chromatographie - Partie 2: Détermination du fer chélaté [o,o] EDDHA, [o,o] EDDHMA et HBED, ou de la quantité d'agents chélatants, par chromatographie d'appariement d'ionsFertilizers - Determination of chelating agents in fertilizers by chromatography - Part 2: Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography71.040.50Fizikalnokemijske analitske metodePhysicochemical methods of analysis65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 13368-2:2017SIST EN 13368-2:2018en,fr,de01-januar-2018SIST EN 13368-2:2018SLOVENSKI
STANDARDSIST EN 13368-2:20131DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 13368-2
November
t r s y ICS
x wä r z r Supersedes EN
s u u x zæ tã t r s tEnglish Version
Fertilizers æ Determination of chelating agents in fertilizers by chromatography æ Part
tã Determination of Fe chelated of chelating agentsá by ion pair chromatography Engrais æ Détermination des agents chélatants dans les engrais par chromatographie æ Partie
t ã Détermination de la quantité d 5agents chélatantsá par chromatographie d 5appariement d 5ions
Düngemittel æ Bestimmung von Chelatbildnern in Düngemitteln mit Chromatographie æ Teil
tã EDDHMA und HBEDá oder der Summe der Chelatbildnerá mit IonenæPaarchromatographie This European Standard was approved by CEN on
s s September
t r s yä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
t r s y CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s u u x zæ tã t r s y ESIST EN 13368-2:2018
Statistical results of the inter-laboratory tests . 19 A.1 Statistical results of the inter-laboratory test performed in 2003 . 19 A.1.1 Test samples . 19 A.1.2 Inter-laboratory test procedure . 19 A.1.3 Results and statistical interpretation . 19 A.2 Statistical results of the inter-laboratory test performed in 2010 . 21 A.2.1 Test samples . 21 A.2.2 Inter-laboratory test procedure . 21 A.2.3 Results and statistical interpretation . 21 SIST EN 13368-2:2018
General procedure for the determination of the titrimetric purity of the chelating agents using a photometric automatic titrator . 26 Annex C (informative)
Complete names of chelating agents . 27 Bibliography . 28 SIST EN 13368-2:2018
1) SYMMETRYTM C18, from WATERS, LiChroCART® Purospher® RP-18, from MERCK or equivalent are examples of suitable products available commercially. This information is given for the convenience of users of this European Standard and does not constitute an endorsement by CEN of these products. SIST EN 13368-2:2018
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記事のタイトル：SIST EN 13368-2:2018 - 肥料 - クロマトグラフィーによる肥料中のキレート剤の定量 - パート2：[o,o] EDDHA、[o,o] EDDHMA、およびHBEDによってキレートされた鉄の定量またはイオンペアクロマトグラフィーによるキレート剤の量の測定 記事の内容：この欧州規格は、肥料中の各個別のオルト（ヒドロキシ）-オルト（ヒドロキシ）異性体によってキレートされた鉄のクロマトグラフィーによる定量方法を規定しています。ただし、[o,o] EDDHMAとHBEDの混合物を除き、これらの物質を含む肥料に適用されます。この方法では、これらのキレート剤の水溶性鉄キレートの識別と総濃度の測定が可能です。また、[o,o] EDDHA、[o,o] EDDHMA、またはHBEDを含む肥料において、Feを誘導した場合、他の微量栄養素が存在する場合にキレート剤の可溶性量を測定することも可能です。 この方法は、規制（EC）No 2003/2003によってカバーされるEC肥料に適用されます。金属キレートの質量分率が少なくとも0.625％である場合に適用されます。 注1：EDDHA（エチレンジアミン-N,N'-ジ（ヒドロキシフェニル）酢酸）やEDDHMA（エチレンジアミン-N,N'-ジ（ヒドロキシメチルフェニル）酢酸）は、いくつかの異性体形態で存在します。ヒドロキシル基やメチル基の位置異性体（ortho、meta、およびpara位置）や立体異性体（メソおよびdl-ラセミ体）が存在することが知られています。[ortho,ortho] EDDHAと[ortho,ortho] EDDHMAのメソおよびdl-ラセミ体は、規制（EC）No 2003/2003で許可されるヒドロキシル基の位置異性体です。EDDHMAのpara、meta、orthoメチル位置異性体は安定性が非常に似ているため、この方法では、[o,o] EDDHMAのpara、meta、orthoメチル位置異性体を一緒に考慮しています。HBED（N,N'-ビス（2-ヒドロキシベンジル）-エチレンジアミン-N,N'-ジ酢酸）には異性体形態が存在しません。 注2：現在、[ortho,ortho] EDDHA、[ortho,ortho] EDDHMA、およびHBEDの分析用純粋基準のみが存在します。その他の物質に対しては基準がないため、それらの物質の存在が試料に及ぼす影響（この方法の感度と選択性に関して）は研究されていません。 注3：[o,o] EDDHAと[o,o] EDDHMAのメソおよびdl-ラセミ体は、この方法によって個別に測定することができます。
The article discusses the European Standard SIST EN 13368-2:2018, which provides a method for determining the iron chelated by different chelating agents in fertilizers. The method is applicable to fertilizers containing substances like EDDHA, EDDHMA, and HBED. It allows for the identification and measurement of the total concentration of water soluble iron chelates of these chelating agents. The method can also be used to determine the soluble amount of chelating agents when other micro-nutrients are present in the fertilizers. The standard is applicable to EC fertilizers and requires a minimum mass fraction of 0.625% for the metal chelated. The article also mentions the different isomeric forms of EDDHA and EDDHMA and the lack of standards for other substances, which affects the method's sensitivity and selectivity. Additionally, the method allows for the separate determination of the meso and dl-racemic forms of EDDHA and EDDHMA.
기사 제목: SIST EN 13368-2: 2018 - 비료 - 크로마토그래피를 통한 비료 중 키렐레이팅제의 결정 - 제2부: [o,o] EDDHA, [o,o] EDDHMA 및 HBED에 의해 키렐레이트된 철 또는 키렐레이트제의 양을 이온 페어 크로마토그래피를 통해 결정 기사 내용: 이 유럽 표준은 [o,o] EDDHA, [o,o] EDDHMA와 HBED의 각 개별 올토 (하이드록시) - 올토 (하이드록시) 이성질체에 의해 키렐레이트 된 철의 크로마토그래피 분석 방법을 규정한다. 이 방법은 [o,o] EDDHA, [o,o] EDDHMA 또는 HBED를 포함하는 비료에 대해 이러한 키렐레이팅제의 수용성 철 금속 키렐레이트의 총 농도를 식별하고 결정하는 것을 가능하게 한다. 또한, Fe로 유도 된 경우 [o, o] EDDHA, [o, o] EDDHMA 또는 HBED를 포함하는 비료에서 철 이외의 미량영양소의 존재시 키렐레이팅제의 용해도를 결정할 수 있다. 이 방법은 2003/2003 EC 규정에 따라 적용 가능한 EC 비료에 적용된다. 최소 0.625%의 금속 키렐레이트 질량 분율에 적용된다. 참고 1 - EDDHA (에디렌디아민-N, N'-di [(hydroxyphenyl)acetic acid]와 EDDHMA (에디렌디아민-N, N'-디 [(hydroxymethylphenyl)acetic acid]은 여러 다른 이성질체 형태로 존재한다. 하이드록실 또는 메틸 기 - (오토, 메타 및 파라 위치)를위한 위치 이성질체뿐만 아니라 구조 이성질체 (메소 및 dl-라세믹 형태)가 알려져 있다. [ortho,ortho] EDDHA와 [ortho,ortho] EDDHMA의 메소 및 dl-라세믹 형태 모두 2003/2003 EC 규정에서 허용 된 하이드록실 기의 위치 이성질체이다. EDDHMA의 파라, 메타 및 올토 메틸 위치 이성질체는 안정성이 매우 유사하기 때문에 이 방법에서는 [o,o] EDDHMA의 파라, 메타 및 올토 메틸 위치 이성질체를 함께 고려한다. HBED (N,N-bis(2-hydroxybenzyl)-ethylenediamine-N,N'-diacetic acid)는 이성질체 형태를 가지지 않는다. 참고 2 - 현재, [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA 및 HBED에 대한 분석적으로 순수한 표준만 존재한다. 그 외의 물질에 대한 분석적으로 순수한 표준은 없으므로 이러한 물질이 샘플에 존재하는 경우 (이 방법의 감도 및 선택도에 대한) 영향을 조사하지 않았다. 참고 3 - [o,o] EDDHA와 [o,o] EDDHMA의 메소 및 dl-라세믹 형태는이 방법을 통해 별도로 결정할 수 있다.
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