Fertilizers - Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT) - Method using high-performance liquid chromatography (HPLC)

This European Standard specifies a method for the quantitative determination of the urease inhibitors N-(n-Butyl)thiophosphoric acid triamide (NBPT, CAS-No. 94317-64-3) and N-(n-Propyl)thiophosphoric acid triamide (NPPT, CAS-No. 916809-14-8) content in urea based fertilizers using high-performance liquid chromatography (HPLC).

Düngemittel - Bestimmung von N-(n-Butyl)thiophosphorsäuretriamid (NBPT) und N-(n-Propyl)thiophosphorsäuretriamid (NPPT) - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)

Diese Europäische Norm legt ein Verfahren zur quantitativen Bestimmung des Gehalts der Ureasehemmstoffe N (n Butyl)thiophosphorsäuretriamid (NBPT, CAS-Nr. 94317 64 3) und N-(n-Propyl)thiophosphorsäuretriamid (NPPT, CAS-Nr. 916809 14 8) in harnstoffbasierten Düngemitteln mit Hochleistungs-Flüssigchromatographie (HPLC) fest.

Engrais - Dosage du acide triamide (NBPT) et de N-(n-propyl) triamide de l'acide thiophosphorique (NPPT) - Méthode par chromatographie liquide à haute performance (HPLC)

La présente norme européenne spécifie une méthode de détermination quantitative de la teneur en inhibiteurs d'uréase N-(n-butyl)triamide de l'acide thiophosphorique (NBPT, N° CAS 94317-64-3) et N-(n-propyl)triamide de l'acide thiophosphorique (NPPT, N° CAS 916809-14-8) dans les engrais à base d'urée, par chromatographie liquide à haute performance (HPLC).

Gnojila - Določevanje N-(n-butil)-triamida tiofosforne kisline (NBPT) in N-(n-propil)-triamida tiofosforne kisline (NPPT) - Metoda tekočinske kromatografije visoke ločljivosti (HPLC)

Ta dokument določa metodo kvantitativnega določanja vsebnosti inhibitorjev sečnine N-(n-butil)-triamida tiofosforne kisline (NBPT, št. CAS 94317-64-3) in N-(n-propil)-triamida tiofosforne kisline (NPPT št. CAS 916809-14-8) v gnojilih, ki temeljijo na sečnini, z metodo tekočinske kromatografije visoke ločljivosti (HPLC).

General Information

Status
Published
Public Enquiry End Date
03-Aug-2014
Publication Date
09-Aug-2015
ICS
65.080 - Fertilizers
Technical Committee
KAT - Soil quality
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
08-Jul-2015
Due Date
12-Sep-2015
Completion Date
10-Aug-2015
Directive
2003/2003 - Regulation (EC) N° 2003/2003 of the European Parlament and of the council of 13 October 2003 relating to fertilisers
Mandate
M/418 - Standardization mandate M/335 assigned to CEN concerning the modernisation of the methods of analysis of fertilizers
Ref Project
EN 16651:2015 - Fertilizers - Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT) - Method using high-performance liquid chromatography (HPLC)

Overview

EN 16651:2015 (CEN) specifies a validated analytical method for the quantitative determination of the urease inhibitors NBPT (N-(n‑Butyl)thiophosphoric acid triamide, CAS 94317‑64‑3) and NPPT (N-(n‑Propyl)thiophosphoric acid triamide, CAS 916809‑14‑8) in urea‑based fertilizers. The method uses high‑performance liquid chromatography (HPLC) with UV detection (205 nm) and provides procedures for sample preparation, calibration, calculation of results and precision assessment.

Key topics and technical requirements

  • Scope: Quantitative determination of NBPT and NPPT in urea fertilizers.
  • Analytical principle: HPLC analysis of aqueous extracts with UV detection at 205 nm.
  • Typical HPLC conditions:
    • Example column: LiChroSpher 100 RP‑8 (250 mm × 4 mm, 5 μm).
    • Mobile phase (eluent): 750 ml water + 250 ml acetonitrile (per 1 000 ml), filtered.
    • Flow rate: 1.0 ml/min; injection 20 μl; expected retention times: NPPT ≈ 5 min, NBPT ≈ 7 min.
  • Reagents and standards: HPLC‑grade acetonitrile, grade 1 water (EN ISO 3696), analytical NBPT and NPPT standards; prescribed stock and calibration solutions (C1, C2, C3) with defined concentrations.
  • Sample preparation: Example test portion - weigh 7.500 g sample into 250 ml flask, dissolve in water, filter (0.45 μm) and analyze within 24 hours.
  • Calibration and calculation: External calibration using three standards; calculation of response factors and expression of results as mass fraction (%) for NBPT and NPPT; total active content = NBPT + NPPT.
  • Precision: Inter‑laboratory trial (2013, 11 labs) and statistical precision data (repeatability and reproducibility) are included; method precision assessed according to ISO 5725‑2.
  • Environmental and safety: Disposal guidance subject to safety data sheets and local regulations.
  • Normative references: EN 1482‑2 (sampling/sample prep), EN 12944‑1/2 (vocabulary), EN ISO 3696 (water quality).

Applications and users

  • Quality control laboratories in fertilizer production verifying urease inhibitor content.
  • Independent analytical testing labs certifying product composition and regulatory compliance.
  • Research and development teams assessing stability or formulation of urea‑based fertilizers containing NBPT/NPPT.
  • Regulatory authorities and certification bodies monitoring labeling and performance of urease inhibitors. Practical uses include batch release testing, supplier verification, traceability of active ingredient concentration, and supporting agronomic efficacy claims.

Related standards

  • EN 1482‑2 - Sampling and sample preparation for fertilizers.
  • EN 12944‑1 / EN 12944‑2 - Vocabulary for fertilizers and soil improvers.
  • EN ISO 3696 - Water for analytical laboratory use.

Keywords: EN 16651:2015, NBPT, NPPT, HPLC method, fertilizers, urease inhibitors, urea‑based fertilizers, CEN, calibration, analytical method, quality control.

Frequently Asked Questions

SIST EN 16651:2015 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Fertilizers - Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT) - Method using high-performance liquid chromatography (HPLC)". This standard covers: This European Standard specifies a method for the quantitative determination of the urease inhibitors N-(n-Butyl)thiophosphoric acid triamide (NBPT, CAS-No. 94317-64-3) and N-(n-Propyl)thiophosphoric acid triamide (NPPT, CAS-No. 916809-14-8) content in urea based fertilizers using high-performance liquid chromatography (HPLC).

This European Standard specifies a method for the quantitative determination of the urease inhibitors N-(n-Butyl)thiophosphoric acid triamide (NBPT, CAS-No. 94317-64-3) and N-(n-Propyl)thiophosphoric acid triamide (NPPT, CAS-No. 916809-14-8) content in urea based fertilizers using high-performance liquid chromatography (HPLC).

SIST EN 16651:2015 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers. The ICS classification helps identify the subject area and facilitates finding related standards.

SIST EN 16651:2015 is associated with the following European legislation: EU Directives/Regulations: 2003/2003; Standardization Mandates: M/418. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.

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Standards Content (Sample)


2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von N-(n-Butyl)thiophosphorsäuretriamid (NBPT) und N-(n-Propyl)thiophosphorsäuretriamid (NPPT) - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)Engrais - Dosage du acide triamide (NBPT) et de N-(n-propyl) triamide de l'acide thiophosphorique (NPPT) - Méthode par chromatographie liquide à haute performance (HPLC)Fertilizers - Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT) - Method using high-performance liquid chromatography (HPLC)65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16651:2015SIST EN 16651:2015en,fr,de01-september-2015SIST EN 16651:2015SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16651
May 2015 ICS 65.080 English Version
Fertilizers - Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT) - Method using high-performance liquid chromatography (HPLC)
Engrais - Dosage du N-(n-butyl)triamide de l'acide thiophosphorique (NBPT) et du N-(n-propyl)triamide de l'acide thiophosphorique (NPPT) - Méthode par chromatographie liquide à haute performance (HPLC)
Düngemittel - Bestimmung von N-(n-Butyl)thiophosphorsäuretriamid (NBPT) und N-(n-Propyl)thiophosphorsäuretriamid (NPPT) - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC) This European Standard was approved by CEN on 16 April 2015.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2015 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16651:2015 ESIST EN 16651:2015

Results of the inter-laboratory test . 10 Bibliography . 11
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