SIST EN 1899-2:2000

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wasserbeschaffenheit - Bestimmung des biochemischen Sauerstoffbedarfs nach n Tagen (BSBn) - Teil 2: Verfahren für unverdünnte Proben (ISO 5815:1989, modifiziert)Qualité de l'eau - Détermination de la demande biochimique en oxygene apres n jours (DOBn) - Partie 2: Méthode pour les échantillons non dilués (ISO 5815:1989, modifiée)Water quality - Determination of biochemical oxygen demand after n days (BODn) - Part 2: Method for undiluted samples (ISO 5815:1989, modified)13.060.50VQRYLExamination of water for chemical substancesICS:Ta slovenski standard je istoveten z:EN 1899-2:1998SIST EN 1899-2:2000en01-januar-2000SIST EN 1899-2:2000SLOVENSKI
STANDARD



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



SIST EN 1899-2:2000



INTERNATIONAL STANDARD 5815 Second edition 1989-08-01 -- Water quality - Determination of biochemical oxygen demand after 5 days (BODs) - Dilution and seeding method Qua/it@ de l’eau - Dktermination de la demande biochimigue en oxyghne aprks 5 jours fDBOS) - Mkthode par dilution et ensemencement Reference number IS0 5815 : 1989 (E) SIST EN 1899-2:2000



Is0 5815 : 1989 (El Foreword IS0 (the International Organization for Standardization) is a worldwide federation of national standards bodies (IS0 member bodies). The work of preparing International Standards is normally carried out through IS0 technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with ISO, also take part in the work. IS0 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the IS0 Council. They are approved in accordance with IS0 procedures requiring at least 75 % approval by the member bodies voting. International Standard IS0 5815 was prepared by Technical Committee ISO/TC 147, Water quality. This second edition constitutes a minor replaces cancels and revision. the first edition (IS0 5815 : 19831, of which it Annex A of this International Standard is for information only. 0 IS0 1989 All rights reserved. No part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization Case postale 56 l CH-121 1 Genkve 20 l Switzerland Printed in Switzerland SIST EN 1899-2:2000



INTERNATIONAL STANDARD IS0 5815-1989 (E) Water quality - Determination of biochemical oxygen demand after 5 days (BOD5) - Dilution and seeding method 1 Scope This International Standard specifies a method for the empirical and conventional determination of the biochemical oxygen demand of waters by dilution and seeding. The method is applicable to all waters having biochemical oxygen demands greater than or equal to 3 mg of oxygen per litre and not exceeding 6 000 mg of oxygen per Iitre. For biochemical oxygen demands greater than 6 000 mg of oxygen per litre, the method is still applicable, but the errors caused by the dilutions necessary require the results to be interpreted with circumspection. The results obtained are the product of a combination of biochemical and chemical actions. They do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defined, chemical process. Never- theless, they provide an indication from which the quality of waters can be estimated. The test may be influenced by the presence of various substances. Those which are toxic to micro-organisms, for example bactericides, toxic metals or free chlorine, will inhibit biochemical oxidation. The presence of algae or nitrifying micro-organisms may produce artificially high results. Annex A gives infor and temperatur .es. mation on alternative incubation periods 2 Normative references I SQ 7393-l : 1985, Water quality - Determination of free chlorine and total chlorine - Part I : Titrime tric method using N, N-diethyl- 7,4phenylenediamine. IS0 7393-2 : 1985, Water quality - Determination of free chlorine and total chlorine - Part 2 : Calorimetric method using N , N-die thy/- 1,4phenylenediamine, for routine con trof purposes. 3 Definition For the purposes definition applies. of this International Standard, the following biochemical oxygen demand IBOD) : The mass concentra- tion of dissolved oxygen consumed under specified conditions by the biological oxidation of organic and/or inorganic matter in water. (Definition taken from IS0 6107-2.) For the purpose of this In ternational Sta ndard, “biological oxidation” is take n to mean “biochemical o xidatio n”. 4 Principle The following standards contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publication, the editions indicated were valid. All standards are subject to revision’ and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of IEC and IS0 maintain registers of currently valid International Stan- dards. Neutralization of the sample of water to be analysed and dilu- tion with varying amounts of a dilution water rich in dissolved oxygen and containing a seed of aerobic micro-organisms, with or without suppression of nitrification, as desired. I SO 5813 : 1983, Water quality -- Determination of dissolved oxygen - lodometric method. IS0 5814 : 1984, Water quality - Determination of dissolved oxygen - Electrochemical probe method. IS0 6107-2 : 1981 f Water quality - Vocabulary - Part 2. Incubation at a controlled temperature for a defined period, 5 days, in the dark, in a completely filled and stoppered bottle. Determination of the dissolved oxygen concentration before and after incubation. Calculation of the mass of oxygen con- sumed per litre of water. Simultaneous performance of a check tion of glucose and glutamic acid. 5 Reagents test on a standard solu- During the analysis’ use only reagents of recognized analytical grade and only distilled water or water of equivalent purity (water distilled in an all-glass apparatus or demineralized water 1. 1 SIST EN 1899-2:2000



IS0 5815-1989 (El The water shall not contain more than 0,Ol mg of copper per litre, and shall be free from chlorine, chloramines, caustic alkalinity, organic matter and acids. 5.1 Seeding water. If the test sample does not contain, by itself, sufficient adapted micro-organisms, seeding water, obtained in one of the follow- ing ways, shall be used: a) Urban waste water, drawn from a main sewer or from a sewer of a residential zone free from marked industrial con- tamination This water shall be decanted before use. b) Add 100 g of garden soil to 1 litre of water. Mix and allow to stand for 10 min. Take 10 ml of the supernatant liquid and make up to 1 litre with water. c) River or lake water containing urban waste water. d) Settled effluent from a waste water treatment plant. e) Water taken downstream from the discharge of the water to be analysed or water containing micro-organisms adapted to the water to be analysed and cultivated in the laboratory (case of industrial effluents containing sub- stances which degrade with difficulty). 5.2 Salt solutions. The following solutions are stable for at least 1 month and should be stored in glass bottles in the dark. They should be discarded at the first sign of precipitation or biological growth. 52.1 Phosphate, buffer solution. Dissolve 8,5 g of potassium dihydrogenphosphate (KH,P04)f 21,75 g of dipotassium hydrogenphosphate ( KZHP04), 33,4 g of disodium hydrogenphosphate heptahydrate (Na2HP04~7H20) and 1,7 g of ammonium chloride (NH&I) in about 500 ml of water. Dilute to 1 000 ml and mix. NOTE - The pH of this buffer solution should be 7’2 without further adjustment. 5.2.2 Wlagnesium sulfate heptahydrate, 22,5 g/l solution. Dissolve 22’5 g of magnesium sulfate heptahydrate (MgSO,a 7H20) in water. Dilute to 1 000 ml and mix. 5.2.3 Calcium chloride, 27,5 g/l solution. Dissolve 27,5 g .of anhydrous calcium chloride (CaC12) (or equivalent, if hydrated calcium chloride is used) in water. Dilute to 1 000 ml and mix. 5.2.4 Iron(lll) chloride hexahydrate, Of25 g/l solution. Dissolve Of25 g of iron(lll) chloride hexahydrate (FeCls.6HZO) in water. Dilute to 1 000 ml and mix. 5.3 Dilution water. Add to about 500 ml of water 1 ml of each of the salt solutions (5.2.1, 5.2.2, 5.2.3 and 5.2.4). Dilute to 1 000 ml and mix. Bring the solution thus obtained to a temperature of about 20 OC and keep at this temperature; aerate for 1 h, taking every pre- caution not to contaminate it, in particular by the addition of organic matter, oxidizing or reducing substances, or metalst) to ensure that the dissolved oxygen concentration is at least 8 mg/l. Use this solution within 24 h of preparation and discard any re- maining solution at the end of the working period. 5.4 Seeded dilution water. Add, according to its source 5 ml to 20 ml of the seeding water (5.1) per litre of dilution water (5.3). Store the seeded dilution water thus obtained at about 20 OC. Prepare immediately before use and discard any remaining solution at the end of the working day. The oxygen depletion over 5 days, at 20 OC of the seeded dilu- tion water (5.4)’ which is the blank value (8.3)’ shall preferably not exceed Of5 mg of oxygen per litre. 5.5 Hydrochloric acid (HCI), 0,5 mol/l. 5.6 Sodium hydroxide (NaOH 20 g/l. 5.7 Sodium sulfite (Na$O,), 0,5 mol/l. solution, approximately ), solution, approximately , solution, approximately 5.8 Glucose-glutamic acid, standard solution. Dry some dehydrated glucose (CGH1206) and some g acid (HOOC-CHZ-CH2-CHNH,-COOH) at 103 OC for 1 h. 150 + 1 mg of each, dissolve in water, dilute to 1 000 mix. Prepare the solution i mmediately before use and discard maining solution at t he end of the working day. lutamic Weigh ml and any re- 5.9 Allylthiourea (ATU) (C,H,N$), solution. Dissolve 1’00 g of allylthiourea in water, dilute to 1 000 ml and mix. The solution is stable for at least 2 weeks. I) It is recommended that either a bottle of compressed air be used, or a compressor fluid (compressors using diaphragm pumps) . Filter and wash the air before use. in which the air does not come into contact with any lubricating 2 SIST EN 1899-2:2000



IS0 5815-1989 (E) Table 1 - Recommended dilutions for determination of BOD, 4to 12 10 to 30 20 to 60 40 to 120 100 to 300 between 1 and 2 * R : river water; E : biologically purified sewage water; S : clarified s
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