SIST EN ISO 16264:2004

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wasserbeschaffenheit - Bestimmung löslicher Silicate mittels Fließanalytik (FIA und CFA) und photometrischer Detektion (ISO 16264:2002)Qualité de l'eau - Dosage des silicates solubles par analyse en flux (FIA et CFA) et détection photométrique (ISO 16264:2002)Water quality - Determination of soluble silicates by flow analysis (FIA and CFA) and photometric detection (ISO 16264:2002)13.060.50VQRYLExamination of water for chemical substancesICS:Ta slovenski standard je istoveten z:EN ISO 16264:2004SIST EN ISO 16264:2004en,fr,de01-junij-2004SIST EN ISO 16264:2004SLOVENSKI
STANDARD



SIST EN ISO 16264:2004



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN ISO 16264February 2004ICS 13.060.50English versionWater quality - Determination of soluble silicates by flow analysis(FIA and CFA) and photometric detection (ISO 16264:2002)Qualité de l'eau - Dosage des silicates solubles par analyseen flux (FIA et CFA) et détection photométrique (ISO16264:2002)Wasserbeschaffenheit - Bestimmung löslicher Silicatemittels Fließanalytik (FIA und CFA) und photometrischerDetektion (ISO 16264:2002)This European Standard was approved by CEN on 2 January 2004.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2004 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 16264:2004: ESIST EN ISO 16264:2004



EN ISO 16264:2004 (E)2ForewordThe text of ISO 16264:2002 has been prepared by Technical Committee ISO/TC 147 "Waterquality" of the International Organization for Standardization (ISO) and has been taken over asEN ISO 16264:2004 by Technical Committee CEN/TC 230 "Water analysis", the secretariat ofwhich is held by DIN.This European Standard shall be given the status of a national standard, either by publication ofan identical text or by endorsement, at the latest by August 2004, and conflicting nationalstandards shall be withdrawn at the latest by August 2004.According to the CEN/CENELEC Internal Regulations, the national standards organizations ofthe following countries are bound to implement this European Standard: Austria, Belgium,Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.Endorsement noticeThe text of ISO 16264:2002 has been approved by CEN as EN ISO 16264:2004 without anymodifications.NOTE
Normative references to International Standards are listed in annex ZA (normative).SIST EN ISO 16264:2004



EN ISO 16264:2004 (E)3Annex ZA(normative)Normative references to international publicationswith their relevant European publicationsThis European Standard incorporates by dated or undated reference, provisions from otherpublications. These normative references are cited at the appropriate places in the text and thepublications are listed hereafter. For dated references, subsequent amendments to or revisions ofany of these publications apply to this European Standard only when incorporated in it byamendment or revision. For undated references the latest edition of the publication referred toapplies (including amendments).NOTE Where an International Publication has been modified by common modifications, indicatedby (mod.), the relevant EN/HD applies.PublicationYearTitleENYearISO 36961987Water for analytical laboratory use -Specification and test methodsEN ISO 36961995SIST EN ISO 16264:2004



SIST EN ISO 16264:2004



Reference numberISO 16264:2002(E)© ISO 2002
INTERNATIONAL STANDARD ISO16264First edition2002-05-15Water quality — Determination of soluble silicates by flow analysis (FIA and CFA) and photometric detection Qualité de l'eau — Dosage des silicates solubles par analyse en flux (FIA et CFA) et détection photométrique
SIST EN ISO 16264:2004



ISO 16264:2002(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
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SIST EN ISO 16264:2004



ISO 16264:2002(E) © ISO 2002 – All rights reserved iii Contents Page Foreword.iv Introduction.v 1 Scope.1 2 Normative references.1 3 General interferences.1 4 Principle.2 5 Reagents.2 6 Apparatus.4 7 Sampling and sample preparation.7 8 Procedure.7 9 Calculation.9 10 Precision.9 11 Test report.10 Bibliography.11
SIST EN ISO 16264:2004



ISO 16264:2002(E) iv © ISO 2002 – All rights reserved
Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 16264 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical, chemical, biochemical methods. SIST EN ISO 16264:2004



ISO 16264:2002(E) © ISO 2002 – All rights reserved v Introduction Further investigation will be necessary to determine whether and to what extent particular problems will require the specification of additional minor conditions. It is absolutely essential that tests conducted according to this International Standard be carried out by suitably qualified staff. Differentiation is required between flow injection analysis (FIA)[1], [2], and continuous flow analysis (CFA) [3]. Both methods share the feature of an automatic dosage of the sample into a flow system (manifold) where the analytes in the sample react with the reagent solutions on their way through the manifold. The sample preparation may be integrated into the manifold. The reaction product is determined in a flow detector (e.g. photometer). This detector produces a signal from which the concentration of the parameter can be calculated. Methods using flow analysis automate wet chemical procedures and are particularly suitable for processing many analytes in water in large sample series at a high analysis frequency.
SIST EN ISO 16264:2004



SIST EN ISO 16264:2004



INTERNATIONAL STANDARD ISO 16264:2002(E) © ISO 2002 – All rights reserved 1 Water quality — Determination of soluble silicates by flow analysis (FIA and CFA) and photometric detection WARNING — Persons using this International Standard should be familiar with normal laboratory practice. This International Standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. 1 Scope This International Standard specifies two methods, i.e. flow injection analysis (FIA) and continuous flow analysis (CFA), for the determination of soluble silicate ions in various types of water (such as ground, drinking, surface, leachate and waste water). Both methods are applicable to the determination of a mass concentration of silicate (SiO2) ranging from 0,2 mg/l to 20 mg/l (with working ranges 0,2 mg/l to 2,0 mg/l and 2 mg/l to 20 mg/l). Other mass concentration ranges are applicable, provided they cover exactly one decade of concentration units (e.g. 0,02 mg/l to 0,2 mg/l in SiO2). These methods can be made applicable to seawater by changing the sensitivity and by adapting the reagent and calibration solutions to the salinity of the samples. 2 Normative references The following normative documents contain provisions which, through reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standards. ISO 3696:1987, Water for analytical laboratory use — Specification and test methods ISO 5725-2:1994, Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method ISO 8466-1:1990, Water quality — Calibration and evaluation of analytical methods and estimation of performance characteristics — Part 1: Statistical evaluation of the linear calibration function 3 General interferences Tannin, large amounts of iron, sulfide and phosphate interfere. The treatment with oxalic acid reduces the interference from tannin and eliminates interference from phosphate provided the mass concentrations are < 20 mg/l. If this value is exceeded, the amount of oxalic acid may not be sufficient to destroy all molybdophosphoric acid being formed. In this case, dilute the sample with water (5.1). When aluminium ions are present in the water sample, the addition of oxalic acid is not completely effective in eliminating phosphate interference. In this case, add 0,2 ml of a 0,1 mol/l sodium cyanide solution per litre of sample prior to analysis. If the sample contains fluoride in concentrations > 150 mg/l, treat the samples with boric acid. High concentrations of oxidizing agents are destroyed by adding 1 mol/l of sodium sulfite solution prior to analysis. If the sample contains large concentrations of sulfide, e.g. > 5 mg/l, special provisions are necessary. SIST EN ISO 16264:2004



ISO 16264:2002(E) 2 © ISO 2002 – All rights reserved
4 Principle 4.1 FIA method The sample is injected into a carrier stream of water by means of a valve. An acidic solution of heptamolybdate is added to the sample and reacts with silicates and phosphates to form molybdosilicate and molybdophosphoric acid. The latter is destroyed by oxalic acid. The molybdosilicate is then reduced to molybdenum blue using a tin(II) chloride solution. The result is expressed in milligrams of SiO2 per litre of water. 4.2 CFA method An acidic solution of heptamolybdate is added to the sample and reacts with silicates and phosphates to form molybdosilicate and molybdophosphoric acid. The latter is destroyed by oxalic acid. The molybdosilicate is then reduced to molybdenum blue using ascorbic acid. The result is expressed in milligrams of SiO2 per litre of water. 5 Reagents Use analytical grade chemicals unless otherwise specified. Check the silicate blank value of the water and of the reagents (8.2.1). Carefully degas all reagent solutions, e.g. by vacuum filtration or by purging with helium (about 1 min). Use only polyethene or polypropene laboratory ware (flasks, beakers and pipettes) for the preparation and storage of the samples (see clause 7), water (5.1) and the solutions 5.8, 5.9 and 5.10. 5.1 Water, complying with purity grade 1 of ISO 3696. 5.2 Sulfuric acid (H2SO4), ρ(H2SO4) = 1,84 g/ml. 5.3 Molybdate solution, (see R1 in Figures 1 and 2). In a 1 000 ml volumetric flask, dissolve (30 ± 0,3) g of hexaammonium heptamolybdate tetrahydrate, (NH4)6Mo7O24⋅4H2O, in about 800 ml of water (5.1). Add 15 ml of sulfuric acid (5.2) and dilute to volume with water. The solution may be stored for up to 3 months at room temperature. Discard the solution if precipitation or coloration occurs. 5.4 Oxalic acid solution, (see R2 in Figures 1 and 2). In a 1 000 ml volumetric flask, dissolve (44 ± 0,4) g of oxalic acid dihydrate, C2H2O4⋅2H2O, in about 800 ml of water (5.1) and dilute to volume with water. The solution may be stored up to 3 months at room temperature. 5.5 Tin(II) chloride reagent, for the determination of silicate by FIA (see R3 in Figure 1). Slowly add 28 ml of sulfuric acid (5.2) to 800 ml water (5.1) in a 1 0
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