Fertilizers - Determination of nitrification inhibitor DMPSA in fertilizers - Method using high-performance liquid chromatography (HPLC)

This document specifies a method for the determination of nitrification inhibitor 2-(3,4-dimethyl-pyrazol-1-yl)-succinic acid (DMPSA) using high-performance liquid chromatography (HPLC), which is applicable to all mineral fertilizers.

Düngemittel - Bestimmung des Nitrifikationshemmstoffs DMPSA in Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)

Dieses Dokument legt ein Verfahren zur Bestimmung des Nitrifikationshemmstoffs 2-(3,4-Dimethyl-pyrazol-1-yl)-bernsteinsäure (DMPSA) mittels Hochleistungs-Flüssigchromatographie (HPLC) fest. Das Verfahren ist für alle mineralischen Düngemittel anwendbar.

Engrais - Dosage de l'inhibiteur de nitrification DMPSA - Méthode par chromatographie liquide à haute performance (HPLC)

Le présent document spécifie une méthode pour le dosage de l’inhibiteur de nitrification acide 2-(3,4-diméthyl-pyrazol-1-yl)succinique (DMPSA) par chromatographie liquide haute performance (HPLC). Elle s’applique à tous les engrais minéraux.

Gnojila - Določevanje inhibitorja nitrifikacije DMPSA v gnojilih - Metoda s tekočinsko kromatografijo visoke ločljivosti (HPLC)

Ta metoda določa metodo za določanje inhibitorja nitrifikacije 2-(3,4-dimetil-pirazol-1-il-sukcinske kisline (DMPSA) s tekočinsko kromatografijo visoke ločljivosti (HPLC). Metoda se uporablja za vsa mineralna gnojila.

General Information

Status
Published
Public Enquiry End Date
09-Apr-2017
Publication Date
31-Jan-2019
ICS
65.080 - Fertilizers
Technical Committee
KAT - Soil quality
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
06-Nov-2018
Due Date
11-Jan-2019
Completion Date
16-Nov-2018
Ref Project
EN 17090:2018 - Fertilizers - Determination of nitrification inhibitor DMPSA in fertilizers - Method using high-performance liquid chromatography (HPLC)

Overview

EN 17090:2018 (CEN) specifies a validated analytical method for the determination of the nitrification inhibitor DMPSA (2-(3,4-dimethyl‑pyrazol‑1‑yl)‑succinic acid) in mineral fertilizers using high‑performance liquid chromatography (HPLC). The standard is applicable to all mineral fertilizers and sets out reagents, apparatus, sample preparation, HPLC conditions, calibration, calculation and reporting requirements for reliable quantification of DMPSA in mg/kg.

Key topics and technical requirements

  • Analyte and scope: Determination of DMPSA in mineral fertilizers. DMPSA occurs as two isomers; the method quantifies the sum of both chromatographic peaks.
  • Sample preparation: Dissolution/extraction in water according to EN 1482-2; filtration (0.45 µm) to remove conditioning agents.
  • Reagents: Grade 3 water (EN ISO 3696), HPLC‑grade acetonitrile, phosphoric acid (85 %), DMPSA reference material (>99 % purity).
  • HPLC conditions (example settings specified):
    • Reverse phase C18 column (e.g. 7 µm, 250 × 4 mm)
    • Eluent: water/acetonitrile 90/10 with 1 ml phosphoric acid
    • Flow rate: 1.0 ml/min; injection volume: 20 µl
    • Detection: UV at 232 nm
    • Expected retention time for DMPSA: ~10–12 min
  • Calibration and quantification: Multi‑point calibration from prepared DMPSA standards; calibration accepted when correlation coefficient r ≥ 0.99. Calculation yields wDMPSA in mg/kg (formula provided in the standard); report mean of duplicate injections to nearest 0.001 g/kg.
  • Quality and precision:
    • Inter‑laboratory trial (2015) with 11 labs provides repeatability (r) and reproducibility (R) limits for tested fertilizer matrices (examples in the standard, e.g., mean values and r/R for NPK, ASN, urea).
    • Repeatability and reproducibility are assessed per ISO 5725-2.

Applications and users

  • Who uses EN 17090:2018:
    • Fertilizer manufacturers and quality control laboratories verifying DMPSA content
    • Accreditation and testing laboratories performing official analyses
    • Regulatory agencies and environmental monitoring bodies checking compliance and label claims
  • Practical uses:
    • Quality assurance of nitrification‑inhibitor‑containing fertilizer products
    • Supporting conformity assessment and regulatory reporting
    • Method transfer and validation in routine analytical labs using HPLC

Related standards

  • EN 1482-2 - Sampling and sample preparation for fertilizers
  • EN 12944-1 / EN 12944-2 - Vocabulary relevant to fertilizers
  • EN ISO 3696 - Water for analytical laboratory use
  • ISO 5725-2 - Determination of repeatability and reproducibility

Keywords: EN 17090:2018, DMPSA, nitrification inhibitor, HPLC, fertilizers, determination, chromatography, CEN, repeatability, reproducibility.

Frequently Asked Questions

SIST EN 17090:2018 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Fertilizers - Determination of nitrification inhibitor DMPSA in fertilizers - Method using high-performance liquid chromatography (HPLC)". This standard covers: This document specifies a method for the determination of nitrification inhibitor 2-(3,4-dimethyl-pyrazol-1-yl)-succinic acid (DMPSA) using high-performance liquid chromatography (HPLC), which is applicable to all mineral fertilizers.

This document specifies a method for the determination of nitrification inhibitor 2-(3,4-dimethyl-pyrazol-1-yl)-succinic acid (DMPSA) using high-performance liquid chromatography (HPLC), which is applicable to all mineral fertilizers.

SIST EN 17090:2018 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers. The ICS classification helps identify the subject area and facilitates finding related standards.

SIST EN 17090:2018 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.

Standards Content (Sample)


2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung des Nitrifikationshemmstoffs DMPSA in Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)Engrais - Dosage de l'inhibiteur de nitrification DMPSA - Méthode par chromatographie liquide à haute performance (HPLC)Fertilizers - Determination of nitrification inhibitor DMPSA in fertilizers - Method using high-performance liquid chromatography (HPLC)65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 17090:2018SIST EN 17090:2018en,fr,de01-december-2018SIST EN 17090:2018SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 17090
November
t r s z ICS
x wä r z r English Version
Fertilizers æ Determination of nitrification inhibitor DMPSA in fertilizers æ Method using highæperformance liquid Engrais æ Dosage de l 5inhibiteur de nitrification DMPSA dans les engrais æ Méthode par chromatographie
Düngemittel æ Bestimmung des Nitrifikationshemmstoffs DMPSA in Düngemitteln æ Verfahren mit HochleistungsæFlüssigchromatographie This European Standard was approved by CEN on
t w June
t r s zä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Serbiaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Rue de la Science 23,
B-1040 Brussels
t r s z CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s y r { rã t r s z ESIST EN 17090:2018

Results of the inter-laboratory test . 8 Table A.1 — Statistical results of the inter-laboratory trial . 8 Bibliography . 9 SIST EN 17090:2018
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