SIST EN 49-2:2015

SLOVENSKI STANDARD
SIST EN 49-2:2015
01-oktober-2015
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SIST EN 49-2:2005
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Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis à vis de
Anobium punctatum (De Geer) par l'observation de la ponte et de la survie des larves -
Partie 2 : Application par imprégnation (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 49-2:2015
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST EN 49-2:2015 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 49-2:2015

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SIST EN 49-2:2015

EUROPEAN STANDARD
EN 49-2

NORME EUROPÉENNE

EUROPÄISCHE NORM
August 2015
ICS 71.100.50 Supersedes EN 49-2:2005
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by egg-
laying and larval survival - Part 2: Application by impregnation
(Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung
l'efficacité protectrice vis à vis de Anobium punctatum (De gegenüber Anobium punctatum (De Geer) durch
Geer) par l'observation de la ponte et de la survie des Beobachten der Eiablage und des Überlebens von Larven -
larves - Partie 2 : Application par imprégnation (Méthode de Teil 2: Anwendung durch Volltränkung
laboratoire) (Laboratoriumsverfahren)
This European Standard was approved by CEN on 16 July 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-2:2015 E
worldwide for CEN national Members.

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SIST EN 49-2:2015
EN 49-2:2015 (E)
Contents Page
European foreword .3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle .5
5 Test materials .6
5.1 Biological material .6
5.2 Products and reagents .6
5.3 Apparatus .6
6 Sampling .7
7 Test specimens .8
7.1 Species of wood.8
7.2 Wood quality .8
7.3 Provision of test specimens .8
7.4 Dimensions of test specimens .8
7.5 Number of test specimens .8
8 Procedure .9
8.1 Preparation of the test specimens .9
8.1.1 Conditioning of test specimens before treatment .9
8.1.2 Treatment of the test specimens .9
8.1.3 Drying and conditioning of the test specimens after treatment . 10
8.2 Exposure of the test specimens to the insects . 11
8.3 Conditions and duration of the test . 11
8.4 Examination of the test specimens . 11
9 Validity of test . 12
10 Expression of results . 12
10.1 Assessment of the protective effectiveness . 12
10.2 Toxic values . 12
11 Test report . 12
Annex A (informative) Example of a test report . 14
Annex B (informative) Identification of sex of test insects (Anobium punctatum) . 16
Annex C (informative) Culturing technique for Anobium punctatum . 17
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory . 20
Bibliography . 21

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SIST EN 49-2:2015
EN 49-2:2015 (E)
European foreword
This document (EN 49-2:2015) has been prepared by Technical Committee CEN/TC 38 “Durability of wood
and wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2016 and conflicting national standards shall be withdrawn
at the latest by February 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 49-2:2005.
Significant technical differences between this document and EN 49-2:2005 are as follows:
a) generalization of material for preparing the egg-laying zones;
b) introduction of new harmonized specifications for wood quality.
EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum (De
Geer) by egg-laying and larval survival, consists of two parts:
— Part 1: Application by surface treatment (Laboratory method);
— Part 2: Application by impregnation (Laboratory method).
EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to be
applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
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Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the
concentration at which the product prevents the development of infestation from egg laying.
The method simulates conditions which can occur in practice on timber which has been treated some time
previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In making this
assessment the methods by which the preservative may be applied should be taken into account. It is further
recommended that results from this test should be supplemented by those from other appropriate tests, and
above all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other
products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of
separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for
personnel (see also Annex D for environmental, health and safety precautions).
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SIST EN 49-2:2015
EN 49-2:2015 (E)
1 Scope
This European Standard specifies a method for the determination of the protective effectiveness or the toxic
values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in
wood which has been treated previously by full impregnation. This method is applicable to:
— water-insoluble chemicals which are being studied as active insecticides;
— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates, and
— water-soluble materials, for example salts.
NOTE This method can be used in conjunction with an ageing procedure, for example EN 73.
2 Normative reference
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid,
the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in untreated
control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or
by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test
specimens.
Depending on the test being carried out either
— on a set of test specimens of a susceptible wood species that is impregnated with a solution of the
preservative, or
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— if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that
are impregnated with a series of solutions in which the concentration of preservative is ranged in a given
progression.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected daily from naturally infested wood or laboratory culture (see
Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.2.2 and
C.6) and then checked to ensure that they are undamaged, active, and free from any infestation by mites.
Determine the sex (see Annex B) of the collected and checked adults and place the males and females in
separate containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the end sections of test specimens.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and
insoluble in the product under test.
NOTE Sodium carboxymethyl cellulose, food grade, has been found to be suitable.
5.2.3 Xylene, technical grade, mixed isomers.
5.2.4 Water, complying with grade 3 of EN ISO 3696.
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a
residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the
egg-laying zones.
NOTE Cotton, linen and polyamide-gauze have been proven suitable.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.
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The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that
this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Treatment vessel(s), of a material that does not react with the preservative under test, for example of
glass for organic products and of polyethylene for salts containing fluorine.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
5.3.6 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-fitting
cover and containing supports that will give minimum contact with treated test specimens to be placed on
them. The vessels and supports shall be of a material that does not react with the preservative under test, for
example glass for organic compounds and polyethylene for products containing fluorine.
5.3.7 Vacuum vessel(s), fitted with stopcocks, capable of receiving the treatment vessels (5.3.3).
1)
.
5.3.8 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa
5.3.9 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with
which they come into contact during the test.
5.3.10 Safety equipment and protective clothing and gloves, appropriate for the test product and the test
solvent, to ensure the safety of the operator.
5.3.11 Test containers, suitable for holding the test specimens and of material resistant to the solvents
used, and fitted with perforated covers to provide a good exchange of air.
NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.
5.3.12 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.
5.3.13 X-ray apparatus, (optional) with tungsten-target and beryllium window, with voltage and current
continuously variable in the ranges:
— voltage: 10 kV to 50 kV;
— current: 0 mA to 15 mA.
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and
handled in accordance with any written recommendations from the supplier.
For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.

1) 100 Pa = 1 mbar.
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7 Test specimens
7.1 Species of wood
The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka)
Lieblin) or pedunculate oak (Quercus robur Linnaeus).
2)
Additional tests may be carried out using other timber species but, if so, this should be stated in the test
report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not
have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees
preferably felled in winter. The trees shall be cut immediately after felling and the timber rapidly air-dried or
kiln dried at temperatures below 60 °C. The wood shall not be stored for more than five years.
3)
The wood shall be exclusively sapwood and having between two annual rings per 10 mm and 10 annual
rings per 10 mm.
It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens
4)
Prepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of
2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the
grain. The annual rings shall be parallel to the broad faces (contact angle of less than 5°). Make transverse
cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens
(50 ± 0,5) mm long.
The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock
originally of more than 500 test specimens.
7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be
(50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.
For the purposes of calculating the mass of preservative retained per unit volume of wood (8.1.2.2) the
3
nominal volume of each test specimen shall be taken as 18,75 cm .
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens
Use:
a) five test specimens (see 7.4) for each preservative and each concentration;

2) The growth of young larvae of Anobium punctatum is slow in test specimens from resinous wood. Results from test
specimens in resinous wood should be compared with those obtained from oak test specimens.
3) It is not essential in this test for the starch content to be high.
4) These test specimens may be taken from the trunk of the tree or the large branches.
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b) five untreated control test specimens (see 7.4) for a complete test of any given preservative;
c) five control test specimens (7.4) treated with that solvent or diluent (5.2.4 or 5.2.5) if a solvent or diluent
(water included) is used.
It is advisable to treat more than the specified number of test specimens so that, after weighing, any test
specimens with abnormally high or low retentions can be rejected from the batch.
8 Procedure
8.1 Preparation of the test specimens
8.1.1 Conditioning of test specimens before treatment
Allow the test specimens to condition in the conditioning chamber (5.3.2), for a minimum of two weeks.
8.1.2 Treatment of the test specimens
8.1.2.1 Preparation of the treatment solution
8.1.2.1.1 Solid preservatives
Water-soluble preservatives:
— dissolve the preservative in the water (5.2.4) to the required concentration, or to a series of
concentrations if toxic values are to be determined.
Non-water-soluble preservatives:
— dissolve the preservative in an appropriate solvent (5.2.5) to the required concentration, or to a series of
concentrations if toxic values are to be determined.
8.1.2.1.2 Liquid preservatives
If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a
concentrate or if toxic values are to be determined, dilute the preservative with the diluent to the required
working concentration, using the procedure specified by the manufacturer.
All treatment solutions shall be freshly prepared.
8.1.2.1.3 Toxic values
If toxic values are to be determined, prepare a series of at least five concentrations by mass, distributed
evenly about the expected toxic values. A solvent or diluent control, i.e. treatment at concentration = 0, shall
also be used. If the approximate toxic values are unknown, the concentrations shall form a widely spaced
geometric progression for a first test and a more closely spaced geometric or arithmetic progression for
subsequent tests.
All treatment solutions shall be freshly prepared.
8.1.2.2 Impregnation
Carry out impregnation in ascending order of concentration, starting with the solvent control
(concentration = 0).
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The following procedure ensures the required complete impregnation of test specimens by the test solutions.
For each concentration weigh each test specimen, to the nearest 0,05 g, and then stack the test specimens in
one of the treatment vessels (5.3.3) so that as much of their face as possible is exposed (e.g. by piling them
crosswise). Ballast the stack of test specimens with the weights (5.3.9) to prevent them floating later when the
liquid is admitted.
Place each vessel in one of the vacuum vessels (5.3.7), attach the vacuum pump (5.3.8) and reduce the
pressure to 700 Pa. Maintain this vacuum for 15 min. Observe the proper safety measures for vacuum
vessels. After this period, close the stopcock to the vacuum pump (5.3.8) and open the other stopcock to allow
the solution of preservative to be drawn into the treatment vessel. Keep the test specimens covered
completely by the solution throughout the remainder of the impregnation process.
Next, admit air to bring the vacuum vessel back to atmospheric pressure, remove the treatment vessel with its
submerged test specimens from the vacuum vessel, cover it and leave it for 2 h, adding further solution as
necessary to keep the test specimens fully covered by liquid.
After this impregnation treatment, remove the test specimens one by one, remove the excess liquid from their
surfaces by lightly blotting with filter paper (5.2.6) and immediately weigh each to the nearest 0,05 g.
In the case of water-soluble preservatives, for example salts and organic chemicals which are being studied
as active substances, calculate the mass of active matter retained by each test specimen from the mass of
5)
solution absorbed and its concentration .
In the case of organic formulations and organic water-dispersible formulations the retention is expressed for
each test specimen in terms of the corresponding mass of the formulation retained but, if a concentrate is
supplied, the retention is expressed in terms of the solution prepared ready for use as specified by the
manufacturer.
Calculate the mass of preservative retained per unit volume of wood in kilograms per cubic metre, for each
test specimen.
Calculate the mean mass of preservative retained per unit volume of wood for each set of five test specimens.
8.1.3 Drying and conditioning of the test specimens after treatment
Arrange the impregnated test specimens treated with each preservative concentration on their narrow faces,
resting on two glass rods, not touching each other in the drying vessel (5.3.6). Place the cover on the drying
vessel. Place the drying vessel in the conditioning chamber (5.3.2). Invert the test specimens twice each week
during the subsequent drying period, temporarily removing the cover to perform these operations.
To prevent mould growth on test specimens treated with water-diluted preservatives, place a small dish
containing the xylene (5.2.3) in the drying vessel (5.3.6).
During the first week retain the cover on the drying vessel.
During the second week uncover the drying vessel progressively each day.
From the beginning of the third week leave the drying vessel fully open. Drying shall be complete at the end of
the fourth week.
NOTE The drying and conditioning of the test specimens depend on the nature of the product under test and on the
solvent or diluent used. For slow drying products, it may be necessary to extend the conditioning process.

5) When dealing with preservative formulations whose constituents may be selectively absorbed by wood, it is necessary
to carry out chemical analysis of the solution before and after impregnation. Similarly, analysis is recommended if very
dilute solutions are used.
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If in the case of slow drying products, the conditioning period is extended, the extended conditioning period
shall be stated in the test report.
If the test specimens are to be subject to an ageing procedure, this shall be carried out after this drying
procedure.
8.2 Exposure of the test specimens to the insects
Coat the transverse faces of each test specimen with the paraffin wax (5.2.1) applied as a single brush coat at
70 °C to 90 °C, and allow to dry for 24 h.
Next prepare the egg-laying zones by attaching a piece of the fine cloth (5.2.7) measuring approximately
45 mm × 20 mm on each large face of the test specimen. Use the paste (5.2.2) to attach the cloth and smooth
this out so that the mash openings are not twisted.
Immediately prior to exposure to egg-laying, condition all the test specimens for one week in the testing
chamber (5.3.5).
Place each test specimen in one of the test containers (5.3.11) and add five female insects and at least five
male insects. Cover the container with a disc of filter paper (5.2.6). Keep this
...