SIST-TP CEN/TR 16420:2013

SLOVENSKI STANDARD
01-januar-2013
Analitska metoda za analizo propikonazola v obdelanih vzorcih lesa
Analytical Method for the Analysis of Propiconazole in Treated Wood Samples
Analyseverfahren zum Nachweis von Propiconazol in behandelten Holzproben
Méthode d'analyse du propiconazole dans des échantillons de bois traité
Ta slovenski standard je istoveten z: CEN/TR 16420:2012
ICS:
79.040 Les, hlodovina in žagan les Wood, sawlogs and sawn
timber
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

TECHNICAL REPORT
CEN/TR 16420
RAPPORT TECHNIQUE
TECHNISCHER BERICHT
October 2012
ICS 79.040
English Version
Analytical Method for the Analysis of Propiconazole in treated
Wood Samples
Méthode d'analyse du propiconazole dans des échantillons Analyseverfahren zum Nachweis von Propiconazol in
de bois traité behandelten Holzproben

This Technical Report was approved by CEN on 27 August 2012. It has been drawn up by the Technical Committee CEN/TC 38.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 16420:2012: E
worldwide for CEN national Members.

Contents Page
Foreword .3
1 Scope .4
2 Normative references .4
3 Safety precautions .4
4 Principle .5
5 Reagents .5
6 Apparatus .5
7 Test sample .6
7.1 General .6
7.2 Dry matter content .6
8 Analytical procedure .6
8.1 General .6
8.2 Standard solutions .7
8.3 Determination .7
9 Calculation and expression of results .9
10 Quality assurance .9
11 Precision .9
12 Test report . 10
Bibliography . 11

Foreword
This document (CEN/TR 16420:2012) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
1 Scope
This CEN Technical Report specifies a laboratory method for determining the content of propiconazole in
treated wood using either Gas Chromatography (GC) or High Performance Liquid Chromatography (HPLC).
The method is aiming at determining the treatment quality at the time of treatment.
NOTE 1 Under appropriate circumstances the method is applicable for tebuconazole-treated wood as well as for the
analysis of waste timber with respect to its propiconazole content.
The method has a detection limit lower than 1 µg propiconazole/g and a quantification limit corresponding to
30 µg propiconazole/g of wood material expressed as dry matter. It can be used over a measurement range
up to a propiconazole content of 600 µg/g of dry matter.
NOTE 2 This method may need some modifications with some wood species such as hardwoods.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
EN 212, Wood preservatives – General guidance on sampling and preparation for analysis of wood
preservatives and treated timber
EN 351-2, Durability of wood and wood-based products – Preservative treated solid wood – Part 2: Guidance
on sampling for the analysis of preservative-treated wood
EN 13183-1, Moisture content of a piece of sawn timber – Part 1: Determination by oven dry method
3 Safety precautions
Persons using this method should be familiar with normal analytical laboratory procedures and practice.
This method does not purport to address all safety problems, if any, associated with its use.
It is the responsibility of the user to establish safety and health practices and to ensure compliance with any
European or national regulatory conditions.
4 Principle
Propiconazole is quantitatively extracted from the wood material using methanol. The extracted compounds
are analysed by GC-ECD, GC-MS, GC-NPD, HPLC-UV or HPLC-DAD.
NOTE Other analytical techniques have been reported to be adequately applicable. However, when using other
analytical techniques the comparability to the method described in this CEN Technical Report should be demonstrated.
1) 2)
Neither FID nor NPD detectors are expected to be appropriate detecting devices.
5 Reagents
5.1 General
During the analysis, unless otherwise specified, use only reagents of recognised analytical grade that have
been checked in advance as to not interfere with the analytical results.
5.2 Methanol (CH OH), analytical grade.
NOTE Other solvents may be used instead of methanol (e.g. ethylacetate) as some extraction difficulties may occur
with some wood species (e.g. hardwoods). It is recommended to cross check the extraction efficiency of any other solvent
or solvent mixture with that of methanol.
5.3 Propiconazole [C H Cl N O ] [CAS 60207-90-1] of certified purity:
15 17 2 3 2
 purity >98 % - mixture of stereo isomers (e.g. Pestanal from Sigma-Aldrich, cat. 45642),
 alternatively, (typical purity 50 %) - mixture of stereo isomers, from Janssen Pharmaceutica, supplied
with Certificate of Analysis.
6 Apparatus
Ordinary laboratory apparatus and the following:
6.1 Grinder, capable of grinding timber samples such that the resultant particles will pass through a 1000
µm sieve.
6.2 Analytical balance, accurate to 0,1 mg.
6.3 Ultra-sonic bath equipped with a thermostat capable of controlling and maintaining a temperature of
(50 ± 1) °C.
6.4 Adjustable volumetric micropipettes, 10 µl, 100 µl and 1000 µl full capacity.
6.5 Volumetric pipettes, 10 ml capacity.
6.6 Volumetric flasks, 50 ml capacity.
6.7 Glass tubes 30 ml capacity, with screw caps provided with an insert of polytetrafluoroethylene (PTFE)
or alternatively with standard ground stoppers provided with PTFE standard ground sockets and conical joint
clips.
1) Concluded from the 2007 round robin test.
2) Concluded from the 2008 round robin test.
6.8 PTFE filters 0,45 µm porosity with supporting device.
6.9 2 ml GC vials with sealed caps.
6.10 Gas chromatograph equipped with a splitless/split or a non-discriminating injection system and an
electron capture detector (ECD) for example.
6.11 Semi-polar separation column. One capillary column, or preferably two with a stationary phase of
different polarity; leng
...