Microbeam analysis — Analytical electron microscopy — Methods for calibrating image magnification by using reference materials with periodic structures

ISO 29301:2017 specifies a calibration procedure applicable to images recorded over a wide magnification range in a transmission electron microscope (TEM). The reference materials used for calibration possess a periodic structure, such as a diffraction grating replica, a super-lattice structure of semiconductor or an analysing crystal for X-ray analysis, and a crystal lattice image of carbon, gold or silicon. This document is applicable to the magnification of the TEM image recorded on a photographic film, or an imaging plate, or detected by an image sensor built into a digital camera. This document also refers to the calibration of a scale bar. This document does not apply to the dedicated critical dimension measurement TEM (CD-TEM) and the scanning transmission electron microscope (STEM).

Analyse par microfaisceaux — Microscopie électronique analytique — Méthodes d'étalonnage du grandissement d'image au moyen de matériaux de référence de structures périodiques

General Information

Status
Withdrawn
Publication Date
05-Dec-2017
Current Stage
9599 - Withdrawal of International Standard
Completion Date
16-Oct-2023
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ISO 29301:2017 - Microbeam analysis -- Analytical electron microscopy -- Methods for calibrating image magnification by using reference materials with periodic structures
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INTERNATIONAL ISO
STANDARD 29301
Second edition
2017-12
Microbeam analysis — Analytical
electron microscopy — Methods
for calibrating image magnification
by using reference materials with
periodic structures
Analyse par microfaisceaux — Microscopie électronique analytique
— Méthodes d'étalonnage du grandissement d'image au moyen de
matériaux de référence de structures périodiques
Reference number
ISO 29301:2017(E)
©
ISO 2017

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ISO 29301:2017(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
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copyright@iso.org
www.iso.org
ii © ISO 2017 – All rights reserved

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ISO 29301:2017(E)

Contents Page
Foreword .iv
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Image magnification . 5
4.1 Definition of the image magnification . 5
4.2 Expressing magnification . 6
5 Reference materials . 6
5.1 General . 6
5.2 Requirements for CRM/RM. 7
5.3 Storage and handling . 7
6 Calibration procedures . 7
6.1 General . 7
6.2 Mounting CRM/RM . 8
6.3 Setting TEM operating conditions for calibration . 8
6.4 Capturing digitized image . 9
6.5 Digitizing the image recorded on photographic film .10
6.5.1 General.
...

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