ISO/FDIS 25353
(Main)Textiles — Qualitative proteomic analysis of protein fibres from leather — Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
- Abstract
This document specifies a qualitative procedure to qualifying leather animal species of (fibrous) protein from leather by MALDI-TOF mass spectrometer (MS). The composition of other fibres can be measured by methods described in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see Annex C as an example of mixtures of (fibrous) protein from leather and polyester). The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis of their morphology, according to ISO/TR 11827.
- Status
- Not Published
- Technical Committee
- ISO/TC 38 - Textiles
- Drafting Committee
- ISO/TC 38/WG 22 - Composition and chemical testing
- Current Stage
- 5020 - FDIS ballot initiated: 2 months. Proof sent to secretariat
- Start Date
- 26-Aug-2026
- Completion Date
- 26-Aug-2026
Buy Documents
ISO/FDIS 25353 - Textiles — Qualitative proteomic analysis of protein fibres from leather — Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
REDLINE ISO/FDIS 25353 - Textiles — Qualitative proteomic analysis of protein fibres from leather — Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
ISO/FDIS 25353 - Textiles — Analyse protéomique qualitative de fibres protéiques issues du cuir — Détection des peptides par spectrométrie de masse à temps de vol avec ionisation/désorption laser assistée par matrice (MALDI-TOF MS)
ISO/FDIS 25353 is an ISO standard for the qualitative identification of animal species in protein fibres from leather using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). It specifies how to extract the protein fibres, digest them into peptides, and detect species-specific markers so laboratories can identify the leather species in a sample. The document is written for textile and leather testing work, especially where leather fibres are blended with other fibres.
What does ISO/FDIS 25353 specify?
ISO/FDIS 25353 applies to protein fibres from leather and describes how to identify the animal species present in those fibres. It does not cover the other fibres in a blend; those are measured by methods in the ISO 1833 series, and the results are then combined to determine the whole fibre composition. The method starts with preliminary fibre identification by light microscopy according to ISO/TR 11827, so the analyst first sorts the fibres by morphology before running MALDI-TOF MS.
| Annex | What it covers |
|---|---|
| Annex A | Normative animal species-specific marker in MALDI-TOF-MS analysis |
| Annex B | Informative animal species-specific peaks in MALDI-TOF-MS analysis |
| Annex C | Informative quantitative analysis of mixtures of protein fibres from leather and polyester |
The main text is organized into scope, normative references, terms and definitions, symbols and abbreviated terms, principle, reagents, apparatus, preparation of test specimens, procedure, and test report.
What are the key requirements of ISO/FDIS 25353?
ISO/FDIS 25353 is a laboratory method built around protein extraction, enzymatic digestion, and mass-spectrometric marker checking. In practice, the user needs controlled sample preparation, a MALDI-TOF MS instrument, and a way to interpret species-specific peaks.
- Clause 5, Principle - The leather protein fibres are extracted with 2-mercaptoethanol, digested with trypsin, and then analysed by MALDI-TOF MS. This means the sample must be converted into measurable peptide fragments before species identification can happen.
- Clauses 6 and 7, Reagents and apparatus - The method uses analytical-grade reagents, including water per ISO 3696, ammonium bicarbonate, trypsin, 2-mercaptoethanol, acetonitrile, trifluoroacetic acid and CHCA matrix, together with a MALDI-TOF mass spectrometer, heating equipment, pipettes, a balance, a thermomixer and a spotting plate. In practice, a lab needs both biochemical reagents and the right instrument setup.
- Clause 8, Preparation of test specimens - Test specimens are cut and dried according to ISO 4044, then a mass of about 1 g is weighed. This keeps the specimen preparation consistent and representative.
- Clause 9.1, Protein extraction - The specimen is heated repeatedly with buffer at 95 °C, cooled, treated with trypsin at 37 °C, then mixed with CHCA matrix before analysis. This turns the leather protein into peptides that the instrument can detect.
- Clause 9.2, Animal species identification - Species identification is made by checking the presence or absence of species-specific peaks, first the primary peak and then a second type-specific peak. Annex A lists marker values and Annex B shows example peak patterns for cow, water buffalo, goat, sheep, pig, horse and crocodile.
- Clause 10, Test report - The report must identify the document used, describe the sample where possible, record the date and results, include mass spectra, state the animal species, note any quantitative identification where needed, and list deviations or unusual features. This supports traceability and review of the result.
- Annex C - An informative example shows how to analyse mixtures of protein fibres from leather and polyester by removing the polyester first. This is useful when the species test is only one part of a broader fibre composition check.
What terms does ISO/FDIS 25353 define?
ISO/FDIS 25353 uses a small set of terms that are central to the method.
- Protein - polymers of amino acids that play many critical roles in the body; here, they are the material extracted from leather.
- Peptide - a small protein, usually fewer than 50 amino acids long; these are the fragments measured by MALDI-TOF MS.
- Marker - a species-specific monoisotopic peak used for identification; analysts use it to call the leather species.
- MALDI - matrix-assisted laser desorption/ionization, a soft ionization method for mass spectrometry; it helps release peptides into the gas phase.
- TOF-MS - time-of-flight mass spectrometry, where ions are separated by the time they take to reach the detector; this is how the peptide masses are read.
- m/z - mass-to-charge ratio; the document uses this axis to locate the species-specific peaks.
Who uses ISO/FDIS 25353?
ISO/FDIS 25353 is used by laboratories and technical staff who test leather materials, especially when the sample contains leather fibres or leather-spun yarns mixed with other fibres. It is relevant for quality control, material identification, and documenting which animal species is present in a leather-derived fibre. It also supports work where the result must be reported clearly, with spectra and any deviations recorded.
Which standards are used with ISO/FDIS 25353?
ISO/FDIS 25353 cites and relies on several other ISO documents.
- ISO 3696 - provides grade 3 water for reagent preparation.
- ISO 4044 - gives the preparation method for leather chemical test samples.
- ISO 20418-2:2018 - is the source for shared definitions such as protein, peptide and marker.
- ISO/TR 11827 - is used for preliminary fibre identification by light microscopy before the MALDI-TOF MS step.
- ISO 1833 series - provides methods for measuring the composition of the other fibres in a blend.
- ISO 1833-24 - is used in Annex C as the example method for removing polyester before analysing the remaining leather fibres.
What does the ISO/FDIS 25353 document contain?
ISO/FDIS 25353 contains a full laboratory procedure for species identification in protein fibres from leather. It includes the reagent formulations, the apparatus list, specimen preparation, extraction and digestion steps, MALDI-TOF MS analysis, and the contents required in the test report. Annex A gives the normative marker list, Annex B shows example species-specific peak figures, and Annex C presents an informative workflow for leather/polyester mixtures.
Relations
- Effective Date
- 12-Feb-2026
- Effective Date
- 21-Jun-2025
Buy Documents
ISO/FDIS 25353 - Textiles — Qualitative proteomic analysis of protein fibres from leather — Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
REDLINE ISO/FDIS 25353 - Textiles — Qualitative proteomic analysis of protein fibres from leather — Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
ISO/FDIS 25353 - Textiles — Analyse protéomique qualitative de fibres protéiques issues du cuir — Détection des peptides par spectrométrie de masse à temps de vol avec ionisation/désorption laser assistée par matrice (MALDI-TOF MS)
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Frequently Asked Questions
ISO/FDIS 25353 is a draft published by the International Organization for Standardization (ISO). Its full title is "Textiles — Qualitative proteomic analysis of protein fibres from leather — Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)". This standard covers: This document specifies a qualitative procedure to qualifying leather animal species of (fibrous) protein from leather by MALDI-TOF mass spectrometer (MS). The composition of other fibres can be measured by methods described in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see Annex C as an example of mixtures of (fibrous) protein from leather and polyester). The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis of their morphology, according to ISO/TR 11827.
This document specifies a qualitative procedure to qualifying leather animal species of (fibrous) protein from leather by MALDI-TOF mass spectrometer (MS). The composition of other fibres can be measured by methods described in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see Annex C as an example of mixtures of (fibrous) protein from leather and polyester). The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis of their morphology, according to ISO/TR 11827.
ISO/FDIS 25353 is classified under the following ICS (International Classification for Standards) categories: 59.060.01 - Textile fibres in general. The ICS classification helps identify the subject area and facilitates finding related standards.
ISO/FDIS 25353 has the following relationships with other standards: It is inter standard links to FprEN ISO 25353, ISO/IEC 30118-14:2021. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ISO/FDIS 25353 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
FINAL DRAFT
International
Standard
ISO/TC 38
Textiles — Qualitative proteomic
Secretariat: SAC
analysis of protein fibres from
Voting begins on:
leather — Peptide detection using
2026-08-26
matrix-assisted laser desorption/
Voting terminates on:
ionization time-of-flight mass
2026-10-21
spectrometry (MALDI-TOF MS)
Textiles — Analyse protéomique qualitative de fibres protéiques
issues du cuir — Détection des peptides par MALDI-TOF MS
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
FINAL DRAFT
International
Standard
ISO/TC 38
Textiles — Qualitative proteomic
Secretariat: SAC
analysis of protein fibres from
Voting begins on:
leather — Peptide detection using
matrix-assisted laser desorption/
Voting terminates on:
ionization time-of-flight mass
spectrometry (MALDI-TOF MS)
Textiles — Analyse protéomique qualitative de fibres protéiques
issues du cuir — Détection des peptides par MALDI-TOF MS
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms. 2
5 Principle . 2
6 Reagents . 2
7 Apparatus . 3
8 Preparation of test specimens . 3
9 Procedure . 4
9.1 Protein extraction .4
9.2 Animal species identification of protein fibres from leather .4
10 Test report . 4
Annex A (normative) Animal species-specific marker in MALDI-TOF-MS analysis . 5
Annex B (informative) Animal species-specific peaks in MALDI-TOF-MS analysis. 6
Annex C (informative) Quantitative analysis of mixtures of protein fibres from leather and
polyester .13
Bibliography . 14
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 38, Textiles, in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 248, Textiles and textile products, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
Protein fibres from leather are staple fibres obtained by grinding leather scrap or shaving dust. A leather
spun yarn can be produced by spinning these leather fibres with other fibres.
In the protein fibres from leather industries, the identification of animal species is very important to
guarantee the quality of leather products. However, it is difficult to apply the microscopy method (ISO 17131
[1]
) to identify the protein fibres from leather, since it is not able to observe the cross-section structure of
[1]
leather or its surface. In the case of ISO 17131 , it is necessary to compare the material structure with
typical pictures of known samples by means of microscopy. For that reason, it can be difficult to absolutely
determine animal species of protein fibres from leather.
In order to overcome the difficulties of the microscope method, a method of applying matrix-assisted laser
desorption ionization-time of flight mass spectrometry mass spectrometer (MALDI-TOF MS) has been
developed for the discrimination of animal species of protein fibres from leather.
Since leather is mostly composed of protein, it utilizes enzymes to break down proteins into peptides and
analyse their mass spectra.
The method solves the problem of difficulty in identifying species when leather is pulverized and used, and
can be seen as an advanced technology in that it can be applied to various species as well as cowhide, which
is widely used.
v
FINAL DRAFT International Standard ISO/FDIS 25353:2026(en)
Textiles — Qualitative proteomic analysis of protein fibres
from leather — Peptide detection using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF MS)
1 Scope
This document specifies a qualitative procedure for identifying the animal species of leather using protein
fibres extracted from leather using matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS).
This document does not cover the composition of other fibres. This can be measured by methods described
in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see
Annex C as an example of mixtures of protein fibres from leather and poly
...
ISO/DISFDIS 25353
ISO/TC 38
Secretariat: SAC
Date: 2026-07-2208-12
Textiles — Qualitative proteomic analysis of protein fibres from
leather — Peptide detection using MALDI-TOF MSmatrix-assisted
laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF MS)
DISTextiles — Analyse protéomique qualitative de fibres protéiques issues du cuir — Détection des peptides par
MALDI-TOF MS
FDIS stage
TTThhhiiisss d d drrraftaftaft i i isss s s suuubbbmmmiiittttttededed t t tooo a pa pa parararallel vallel vallel vooottte e e iiinnn I I ISSSOOO,,, C C CEEENNN.
Voting begins on: 2025-09-25
Voting terminates on: 2025-12-18
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
E-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms . 2
5 Principle . 2
6 Reagents . 2
7 Apparatus . 3
8 Preparation of test specimens . 4
9 Procedure . 4
9.1 Protein extraction . 4
9.2 Animal species identification of protein fibres from leather . 4
10 Test report . 4
Annex A (normative) Animal species-specific marker in MALDI-TOF-MS analysis . 6
Annex B (informative) Animal species-specific peaks in MALDI-TOF-MS analysis . 7
Annex C (informative) Quantitative analysis of mixtures of protein fibres from leather and
polyester . 14
Bibliography . 15
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types of
ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent rights
in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that this
may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 38, Textiles, in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 248, Textiles and textile products, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
Protein fibres from leather are staple fibres obtained by grinding leather scrap or shaving dust. A leather spun
yarn can be produced by spinning these leather fibres with other fibres.
In the protein fibres from leather industries, the identification of animal species is very important to guarantee
[1]
the quality of leather products. However, it is difficult to apply the microscopy method (ISO 17131 (()) to
identify the protein fibres from leather, since it is not able to observe the cross-section structure of leather or
[1]
its surface. In the case of ISO 17131 , it is necessary to compare the material structure with typical pictures
of known samples by means of microscopy. For that reason, it can be difficult to absolutely determine animal
species of protein fibres from leather.
In order to overcome the difficulties of the microscope method, a method of applying matrix-assisted laser
desorption ionization-time of flight mass spectrometry mass spectrometer (MALDI-TOF MS) has been
developed for the discrimination of animal species of protein fibres from leather.
Since leather is mostly composed of protein, it utilizes enzymes to break down proteins into peptides and
analyse their mass spectra.
The method solves the problem of difficulty in identifying species when leather is pulverized and used, and
can be seen as an advanced technology in that it can be applied to various species as well as cowhide, which is
widely used.
v
Textiles — Qualitative proteomic analysis of protein fibres from
leather — Peptide detection using MALDI-TOF MSmatrix-assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-
TOF MS)
1 Scope
This document specifies a qualitative procedure for identifying the animal species of leather using protein
fibres extracted from leather using matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS).
This document does not cover the composition of other fibres. This can be measured by methods described in
the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see Annex
C as an example of mixtures of protein fibres from leather and polyester).
The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis
[2]
of their morphology, according to ISO/TR 11827 ISO/TR 11827.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 4044, Leather — Chemical tests — Preparation of chemical test samples
ISO 20418-2:2018, Textiles — Qualitative and quantitative proteomic analysis of some animal hair fibres — Part
2: Peptide detection using MALDI-TOF MS
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at http://www.iso.org/obphttp://www.iso.org/obp
— IEC Electropedia: available at http://www.electropedia.org/
3.1
protein
polymers of amino acids that play many critical roles in the body
[3]
[SOURCE: ISO 20418-2:2018 ISO 20418-2:2018, 3.2]
3.2
peptide
small protein (3.1) consisting of approximately less than 50 amino acids
[3]
[SOURCE: ISO 20418-2:2018 ISO 20418-2:2018,, 3.3]
3.3
marker
mass to change ratio of animal species specific monoisotopic peak used for identificationNote 1 to entry: See
Clause 4 for an explanation of mass to change retion.identification
N
...
PROJET FINAL
Norme
internationale
ISO/TC 38
Textiles — Analyse protéomique
Secrétariat: SAC
qualitative de fibres protéiques
Début de vote:
issues du cuir — Détection des
2026-08-26
peptides par spectrométrie
Vote clos le:
de masse à temps de vol avec
2026-10-21
ionisation/désorption laser assistée
par matrice (MALDI-TOF MS)
Textiles — Qualitative proteomic analysis of protein fibres
from leather — Peptide detection using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-
TOF MS)
LES DESTINATAIRES DU PRÉSENT PROJET SONT
INVITÉS À PRÉSENTER, AVEC LEURS OBSERVATIONS,
NOTIFICATION DES DROITS DE PROPRIÉTÉ DONT ILS
AURAIENT ÉVENTUELLEMENT CONNAISSANCE ET À
FOURNIR UNE DOCUMENTATION EXPLICATIVE.
OUTRE LE FAIT D’ÊTRE EXAMINÉS POUR
ÉTABLIR S’ILS SONT ACCEPTABLES À DES FINS
INDUSTRIELLES, TECHNOLOGIQUES ET COM-MERCIALES,
AINSI QUE DU POINT DE VUE DES UTILISATEURS, LES
PROJETS DE NORMES
TRAITEMENT PARALLÈLE ISO/CEN
INTERNATIONALES DOIVENT PARFOIS ÊTRE CONSIDÉRÉS
DU POINT DE VUE DE LEUR POSSI BILITÉ DE DEVENIR DES
NORMES POUVANT
SERVIR DE RÉFÉRENCE DANS LA RÉGLEMENTATION
NATIONALE.
Numéro de référence
PROJET FINAL
Norme
internationale
ISO/TC 38
Textiles — Analyse protéomique
Secrétariat: SAC
qualitative de fibres protéiques
Début de vote:
issues du cuir — Détection des
2026-08-26
peptides par spectrométrie
Vote clos le:
de masse à temps de vol avec
2026-10-21
ionisation/désorption laser assistée
par matrice (MALDI-TOF MS)
Textiles — Qualitative proteomic analysis of protein fibres
from leather — Peptide detection using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-
TOF MS)
LES DESTINATAIRES DU PRÉSENT PROJET SONT
INVITÉS À PRÉSENTER, AVEC LEURS OBSERVATIONS,
NOTIFICATION DES DROITS DE PROPRIÉTÉ DONT ILS
AURAIENT ÉVENTUELLEMENT CONNAISSANCE ET À
FOURNIR UNE DOCUMENTATION EXPLICATIVE.
DOCUMENT PROTÉGÉ PAR COPYRIGHT
OUTRE LE FAIT D’ÊTRE EXAMINÉS POUR
ÉTABLIR S’ILS SONT ACCEPTABLES À DES FINS
© ISO 2026 INDUSTRIELLES, TECHNOLOGIQUES ET COM-MERCIALES,
AINSI QUE DU POINT DE VUE DES UTILISATEURS, LES
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
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Publié en Suisse Numéro de référence
ii
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d'application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Symboles et abréviations . 2
5 Principe. 2
6 Réactifs . 2
7 Appareillage . 3
8 Préparation des éprouvettes . 3
9 Mode opératoire . 4
9.1 Extraction des protéines .4
9.2 Identification des espèces animales à l'origine des fibres protéiques issues du cuir .4
10 Rapport d'essai . 4
Annexe A (normative) Marqueur spécifique de l'espèce animale dans l'analyse par MALDI-
TOF MS . 5
Annexe B (informative) Pics spécifiques des espèces animales dans l'analyse par MALDI-TOF MS . 6
Annexe C (informative) Analyse quantitative des mélanges de fibres protéiques issues du cuir
avec des fibres de polyester .13
Bibliographie . 14
iii
Avant-propos
L'ISO (Organisation internationale de normalisation) est une fédération mondiale d'organismes nationaux
de normalisation (comités membres de l'ISO). L'élaboration des Normes internationales est en général
confiée aux comités techniques de l'ISO. Chaque comité membre intéressé par une étude a le droit de faire
partie du comité technique créé à cet effet. Les organisations internationales, gouvernementales et non
gouvernementales, en liaison avec l'ISO participent également aux travaux. L'ISO collabore étroitement avec
la Commission électrotechnique internationale (IEC) en ce qui concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier, de prendre note des différents
critères d'approbation requis pour les différents types de documents ISO. Le présent document a
été rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir
www.iso.org/directives).
L'ISO attire l'attention sur le fait que la mise en application du présent document peut entraîner l'utilisation
d'un ou de plusieurs brevets. L'ISO ne prend pas position quant à la preuve, à la validité et à l'applicabilité de
tout droit de propriété revendiqué à cet égard. À la date de publication du présent document, l'ISO n'avait pas
reçu notification qu'un ou plusieurs brevets pouvaient être nécessaires à sa mise en application. Toutefois,
il y a lieu d'avertir les responsables de la mise en application du présent document que des informations
plus récentes sont susceptibles de figurer dans la base de données de brevets, disponible à l'adresse
www.iso.org/brevets. L'ISO ne saurait être tenue pour responsable de ne pas avoir identifié de tels droits de
brevet.
Les appellations commerciales éventuellement mentionnées dans le présent document sont données pour
information, par souci de commodité, à l'intention des utilisateurs et ne sauraient constituer un engagement.
Pour une explication de la nature volontaire des normes, la signification des termes et expressions
spécifiques de l'ISO liés à l'évaluation de la conformité, ou pour toute information au sujet de l'adhésion de
l'ISO aux principes de l'Organisation mondiale du commerce (OMC) concernant les obstacles techniques au
commerce (OTC), voir le lien suivant: www.iso.org/avant-propos.
Le présent document a été élaboré par le comité technique ISO/TC 38, Textiles, en collaboration avec le
comité technique CEN/TC 248, Textiles et produits textiles, du Comité européen de normalisation (CEN)
conformément à l'Accord de coopération technique entre l'ISO et le CEN (Accord de Vienne).
Il convient que l'utilisateur adresse tout retour d'information ou toute question concernant le présent
document à l'organisme national de normalisation de son pays. Une liste exhaustive desdits organismes se
trouve à l'adresse www.iso.org/members.html.
iv
Introduction
Les fibres protéiques issues du cuir sont des fibres discontinues obtenues par broyage de chutes de cuir ou
de résidus de rasage. Un filé de fibres issues du cuir peut être produit en filant ces fibres issues du cuir avec
d'autres fibres.
Dans les fibres protéiques provenant de l'industrie du cuir, l'identification des espèces animales est très
importante pour garantir la qualité des produits en cuir. Cependant, il est difficile d'appliquer la méthode
[1]
de microscopie (ISO 17131 ) à l'identification des fibres protéiques issues du cuir, car cette méthode ne
[1]
permet pas d'observer la structure de la section du cuir ni sa surface. Dans le cas de l'ISO 17131, il est
nécessaire de comparer par microscopie la structure du matériau avec des images types d'échantillons
connus. Pour cette raison, il peut être difficile de déterminer avec certitude les espèces animales à l'origine
des fibres protéiques issues du cuir.
Pour remédier aux lacunes de la méthode au microscope, une méthode utilisant la spectrométrie de masse
à temps de vol avec ionisation/désorption laser assistée par matrice (MALDI-TOF MS) au moyen d'un
spectromètre de masse a été élaborée pour discriminer les espèces animales à l'origine des fibres protéiques
issues du cuir.
Le cuir étant principalement composé de protéines, ladite méthode utilise des enzymes pour décomposer les
protéines en peptides et analyser leur spectre de masse.
Cette méthode résout les difficultés à identifier les espèces lorsque le cuir est pulvérisé et usagé. Elle peut
être considérée comme une technologie avancée en ce sens qu'elle peut s'appliquer à diverses espèces, ainsi
qu'au cuir de vache qui est largement utilisé.
v
PROJET FINAL Norme internationale ISO/FDIS 25353:2026(fr)
Textiles — Analyse protéomique qualitative de fibres
protéiques issues du cuir — Détection des peptides par
spectrométrie de masse à temps de vol avec ionisation/
désorption laser assistée par matrice (MALDI-TOF MS)
1 Domaine d'application
Le présent document spécifie un mode opératoire qualitatif pour identifier à l'aide de fibres protéiques
issues du cuir les espèces animales utilisées pour le cuir, par spectrométrie de masse à temps de vol avec
ionisation/désorption laser assistée par matrice (MALDI-TOF MS).
Le présent document ne couvre pas la composition des autres fibres. Elle peut être mesurée par les méthodes
décrites dans la série ISO 1833. Les deux résultats sont ensuite combinés pour déterminer la composition
totale des fibres (voir Annexe C pour un exemple de mélanges de fibres protéiques issues du cuir et de
polyester).
La méthode repose sur une identification préliminaire, par microscopie optique, de toutes les fibres
[2]
contenues dans un mélange sur la base de leur morphologie, conformément à l'ISO/TR 11827 .
2 Réfé
...