EN 13651:2001

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Izboljševalci tal in rastni substrati - Ekstrakcija hranil, topnih v kalcijevem kloridu/DTPA (CAT)Bodenverbesserungsmittel und Kultursubstrate - Extraktion von in Calciumchlorid/DTPA (CAT) löslichen NährstoffenAmendements du sol et supports de culture - Extraction des éléments nutritifs solubles dans le chlorure de calcium/DTPA (CAT)Soil improvers and growing media - Extraction of calcium chloride/DTPA (CAT) soluble nutrients65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 13651:2001SIST EN 13651:2002en01-november-2002SIST EN 13651:2002SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 13651September 2001ICS 65.080English versionSoil improvers and growing media - Extraction of calciumchloride/DTPA (CAT) soluble nutrientsAmendements du sol et supports de culture - Extractiondes éléments nutritifs solubles dans le chlorure decalcium/DTPA (CAT)Bodenverbesserungsmittel und Kultursubstrate - Extraktionvon in Calciumchlorid/DTPA (CAT) löslichen NährstoffenThis European Standard was approved by CEN on 11 August 2001.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2001 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 13651:2001 ESIST EN 13651:2002

Results of an interlaboratory trial to determine calcium chloride/DTPA(CAT) soluble nutrients and elements.8Annex B (informative)
Methods of analysis used in the interlaboratory trial.15Bibliography.16SIST EN 13651:2002

3 C in an extraction volume ratio of 1+5.The extracted nutrients are determined by various methods as appropriate (see annex B).5 Reagents5.1 GeneralAll reagents used shall be of recognised analytical quality and water of grade 2 according to EN ISO3696.5.2Dihydrated calcium chloride, CaCl2.2H2O.5.3Diethylenetriaminepentaacetic acid (DTPA), C14H23N3O10.5.4Concentrated extracting solution CaCl2/DTPA, (CAT), dissolve 14,7 g CaCl2.2H2O (5.2) and7,88 g DTPA (5.3) in 800 ml hot water (temperature approximately 80 C) with stirring on a magneticstirrer in a 1000 ml beaker.
At 75 C
10 C the reagents will dissolve within 2 h.
Allow to cool toambient temperature. Transfer the solution to a 1000 ml flask and dilute to the mark with water. TheSIST EN 13651:2002

1,42 g/ml; not less than 65 % mass/volume.5.7Nitric acid, c(HNO3) = 0,5 mol/l, dilute 35 ml nitric acid (5.6) to one litre with water.6 Apparatus6.1GeneralNOTEIt has been found convenient to keep separate sets of glassware for the determinations given in annex B,in order to reduce the possibility of within-laboratory contamination.All glass apparatus and plastic vessels used in the procedure should be subject to an appropriatepreparation procedure in order to keep the risk of contamination as low as possible. It is recommendedthat all vessels (glass and plastic) are cleansed by carefully immersing in warm nitric acid (5.6) for aminimum of 6 h and then rinsed in water.Rubber stoppers, which may contain traces of metals shall not be used. It is recommended to use plasticor any other stopper free of all substances to be analyzed.Borosilicate glassware shall not be used if boron is to be determined.The apparatus consists of the usual laboratory apparatus, and in particular the following :6.2Plastic bottles or containers, sufficiently large (500 ml to 1500 ml) with screw cap to accommodatethe volume of the sample, extractant and at least 10 % air volume.6.3Shaking or mixing machine, capable of holding the plastic bottles or containers (6.2) andmaintaining the sample in suspension without damaging the structure of the sample. The use of ahorizontal table shaker is recommended.6.4Filter paper, cellulose-based ashless types, with a medium pore size of approximately 8 m anddiameter of 150 mm.NOTECentrifugation is an acceptable alternative.6.5Analytical balance with an accuracy of 10 mg.7 Test samplePrepare the laboratory sample in accordance with EN 13040:1999, clause 8, and determine thelaboratory compacted bulk density of the sample in accordance with EN 13040:1999, annex A.SIST EN 13651:2002

3 C.8.1.2 Test samples passing through a 40 mm sieveTake a weight equivalent to 250 ml of the sample volume (EN 13040:1999, clause 8.3) to the nearest 1 gand transfer to the container (6.2). Add 1250 ml of extracting solution (5.5), secure the cap and shake for1 h on the shaking machine (6.3) at 22 C
3 C.8.1.3 FiltrationFilter through filter paper (6.4) discarding at least the first 10 ml. In some cases paper filtration is too slowor even impossible. In such cases alternative procedures for obtaining a clear supernatant are acceptableand the technique used shall be reported. The filtered extract is stable for three days in a hermeticallyclosed polyethylene bottle if stored in a refrigerator at 0 C to 5 C. The filtrate can be stored for longerperiods in a deep freezer at about
18 C.NOTEBefore using a solution that has been frozen, the thawed solution shall be thoroughly mixed to eliminategradient separation that occurs on freezing and subsequent thawing.8.2BlankThe reagent blank test shall be carried out in parallel with the determination, by the same procedure asoutlined in 8.1.1 or 8.1.2 and 8.1.3, using the same quantities of all the reagents as in the determinationbut omitting the test portion.NOTEThe measurement of a blank is introduced to determine the contribution of the extracting solution,glassware and filter paper used.9 Determination of the extracted nutrients and elementsSee annex B.10 Expression of resultsSubtract the values det
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