EN ISO 9936:2016

SLOVENSKI STANDARD
01-junij-2016
1DGRPHãþD
SIST EN ISO 9936:2006
SIST EN ISO 9936:2006/A1:2011
SIST EN ISO 9936:2006/AC:2009
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHWRNRIHURODLQWRNRWULHQRODV
WHNRþLQVNRNURPDWRJUDILMRYLVRNHORþOMLYRVWL,62
Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents
by high-performance liquid chromatography (ISO 9936:2016)
Tierische und pflanzliche Fette und Öle - Bestimmung des Tocopherol- und Tocotrienol-
Gehaltes mittels Hochleistungsflüssigchromatographie (ISO 9936:2016)
Corps gras d'origines animale et végétale -- Détermination des teneurs en tocophérols et
en tocotriénols par chromatographie en phase liquide à haute performance (ISO
9936:2016)
Ta slovenski standard je istoveten z: EN ISO 9936:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 9936
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2016
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 9936:2006
English Version
Animal and vegetable fats and oils - Determination of
tocopherol and tocotrienol contents by high-performance
liquid chromatography (ISO 9936:2016)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination des teneurs en tocophérols et en des Tocopherol- und Tocotrienol-Gehaltes mittels
tocotriénols par chromatographie en phase liquide à Hochleistungsflüssigchromatographie (ISO 9936:2016)
haute performance (ISO 9936:2016)
This European Standard was approved by CEN on 4 March 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9936:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 9936:2016) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2016, and conflicting national standards shall
be withdrawn at the latest by October 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 9936:2006.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 9936:2016 has been approved by CEN as EN ISO 9936:2016 without any modification.
INTERNATIONAL ISO
STANDARD 9936
Third edition
2016-04-01
Animal and vegetable fats and
oils — Determination of tocopherol
and tocotrienol contents by high-
performance liquid chromatography
Corps gras d’origines animale et végétale — Détermination des
teneurs en tocophérols et en tocotriénols par chromatographie en
phase liquide à haute performance
Reference number
ISO 9936:2016(E)
©
ISO 2016
ISO 9936:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved

ISO 9936:2016(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Sampling . 3
8 Preparation of test sample . 3
9 Procedure. 3
9.1 Preparation of calibration solutions . 4
9.1.1 Stock calibration solutions . 4
9.1.2 Standard solution . 4
9.2 Optimization of working parameters . 4
9.3 Preparation of test solution . 5
9.4 Determination . 5
10 Expression of results . 6
11 Precision . 6
11.1 Interlaboratory test. 6
11.2 Repeatability . 6
11.3 Reproducibility . 7
12 Test report . 7
Annex A (informative) Examples of chromatograms . 8
Annex B (informative) Saponification .10
Annex C (informative) Results of interlaboratory tests .12
Bibliography .16
ISO 9936:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical
Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This third edition cancels and replaces the second edition (ISO 9936:2006), which has been technically
revised. It also incorporates the Amendment ISO 9936:2006/Amd.1:2011 and the Technical Corrigendum
ISO 9936:2006/Cor.1:2008. A non-applicability statement for milk and milk products has been added to
the Scope.
iv © ISO 2016 – All rights reserved

INTERNATIONAL STANDARD ISO 9936:2016(E)
Animal and vegetable fats and oils — Determination of
tocopherol and tocotrienol contents by high-performance
liquid chromatography
1 Scope
This International Standard specifies a method for the determination of the contents of free α-, β-, γ-,
and δ-tocopherols and tocotrienols (re
...