EN 16086-1:2011

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Bodenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenverträglichkeit - Teil 1: Wachstumstest mit Chinakohl im TopfAmendements du sol et supports de culture - Détermination de la réponse des plantes - Partie 1: Essai de croissance en pot avec du chou de ChineSoil improvers and growing media - Determination of plant response - Part 1: Pot growth test with Chinese cabbage65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16086-1:2011SIST EN 16086-1:2012en,fr,de01-marec-2012SIST EN 16086-1:2012SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16086-1
November 2011 ICS 65.080 English Version
Soil improvers and growing media - Determination of plant response - Part 1: Pot growth test with Chinese cabbage
Amendements du sol et supports de culture - Détermination de la réponse des plantes - Partie 1: Essai de croissance en pot avec du chou de Chine
Bodenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenverträglichkeit - Teil 1: Wachstumstest mit Chinakohl im Topf This European Standard was approved by CEN on 17 September 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
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Validation . 18Annex B (normative)
Nutrient supply and fist test . 21B.1 Composition of the nutrient solution . 21B.2 Possible adjustments of the nutrient supply during the test . 22B.2.1 Possible reasons for nutritional adjustments . 22B.2.2 Recommendations for nutritional adjustments . 22B.2.3 Supplement to the report . 23B.3 Fist test . 24Bibliography . 25
This test may not be suitable for all growing media since the growing media characteristics (e.g. nutrient content) will vary according to target use and the product is not tested in accordance with the specified use and pack recommendations. This test is not appropriate for the detection of nitrogen immobilization. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 13037, Soil improvers and growing media – Determination of pH
EN 13038, Soil improvers and growing media – Determination of electrical conductivity
EN 13040, Soil improvers and growing media – Sample preparation for chemical and physical tests, determination of dry matter content, moisture content and laboratory compacted bulk density EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 plant response variation in plant germination and/or growth when sown and grown in a growing medium, soil improver or constituent thereof or in an extract obtained from these materials Factors causing negative plant growth cannot be identified nor quantified by applying this method. 3.2 prepared sample portion of the laboratory sample, undiluted or diluted with sphagnum peat at given ratios, fertilized and limed as required SIST EN 16086-1:2012

4.2 Pot experiment with direct use of the prepared sample Sowing a defined quantity of Chinese cabbage into pots containing the prepared sample, cultivating under controlled conditions for a defined period of time and evaluating the plant response by determining the germination rate, fresh weight, abnormalities and overall plant growth. If the presence of graminacious herbicides is suspected, Spring barley shall be used in addition to Chinese cabbage. For testing of other specific effects, the use of additional plant species (for example lettuce) can be considered. 4.3 Pot experiment using an extract of the original sample Mixing the original sample with nutrient solution as an extractant, soaking for 4 h at ambient temperature and collecting the freely available nutrient solution. Filling pots with perlite saturated with the extract and continuing as described under 4.2, irrigating during the test period with a fixed quantity of the extract and afterwards water. If the presence of graminacious herbicides is suspected, Spring barley shall be used in addition to Chinese cabbage. For testing of other specific effects, the use of additional plant species (for example lettuce) can be considered. 5 Choice of methodology For most materials, the pot growth test can be carried out as described in Clause 6. However, for coarse materials such as bark, expanded clay, lava, mineral wool, perlite, polyurethane and pumice with an inherently low water holding capacity which are used as growing media without amendment, this procedure is unsuitable and the extract method described in Clause 7 shall be adopted. 6 Pot experiment with direct use of the prepared sample 6.1 Materials
6.1.1 Water of class 3 According to EN ISO 3696. 6.1.2 Sphagnum peat
Sphagnum peat with a degree of humification of H3 – H5 according to von Post scale, having a pH measured according to EN 13037 of between 3,0 and 4,5, an EC measured according to EN 13038 of between
1 mS m-1 and 5 mS m-1, a particle size of < 10 mm and to which neither lime nor fertilizer has been added. 6.1.3 Fertilized and limed sphagnum peat
Sphagnum peat (see 6.1.2), having a pH measured according to EN 13037 adjusted using ground limestone (see 6.1.4) to a range between 5,5 and 6,5, fertilized with a water soluble “complete” fertilizer with essential SIST EN 16086-1:2012

6.2.2 Sieve with 5 mm mesh size
6.2.3 Circular plant pot Upper diameter (12 ± 0,5) cm , height between 8,5 cm and 9,0 cm, volume between 650 ml and 700 ml, perforated bottom to provide drainage (for example plastic pot used in horticulture). 6.2.4 Saucer Saucer capable of catching all surplus water from the plant container after overhead watering. 6.2.5 Thin fleece or plastic sheet for covering the containers 6.2.6 Testing facility Testing facility capable of maintaining and monitoring the temperature and light intensity specified in 6.4 such as a greenhouse or plant growth room. 6.2.7 Irrigation device for watering the pots For example watering can, greenhouse watering hose 6.2.8 Analytical balance Accuracy 0,01 g, capacity 500 g. 6.3 Preparation of the sample 6.3.1 General preparation Pass the sample through a 20 mm sieve (see 6.2.1). Any foreign material such as plastic, metal or glass retained on the sieve shall be removed and noted. Other material that is retained on the sieve and which is an intrinsic part of the sample shall be physically reduced to parts of similar size as few times as are necessary to permit the entire sample to pass through the sieve. Fibrous materials i.e. coir fibres and straw shall be cut to a length ≤ 20 mm by using scissors. Thoroughly mix the laboratory sample with the broken particles that had been retained on the sieve taking care to minimise physical damage to the sample as a whole. Transportation SIST EN 16086-1:2012

(225 ± 25) mg N · l-1 (for example 1,5 g · l-1 water soluble complete fertilizer N : P2O5 : K2O – 15 : 10 : 20) (see B.1) is added to one litre of prepared sample (according to EN 13040). The dilution ratios in Table 1 are based on horticultural practice in the usage of growing media, growing media constituents, soil improvers and soil improver constituents. In general, it is sufficient to carry out the test using the highest proportion of test material. The second dilution ratio may be helpful for further judging plant response. If required (for example to fulfil certain quality certification requirements or legislation), materials can be tested with other dilution ratios than mentioned in Table 1 or without dilution. It might also be necessary to apply further nutritional adjustments, cases are described in B.2. Any adjustments shall be reported. SIST EN 16086-1:2012

obligatory optional Bark (uncomposted) 50 : 50 25 : 75 Brown coal (lignite) 50 : 50 25 : 75 Cocoa hulls 50 : 50 25 : 75 Coir pith 100 : 0 50 : 50 Coir fibres 50 : 50 25 : 75 Coir chips 50 : 50 25 : 75 Composts made of material such as biodegradable waste, bark, wood, straw, manure 50 : 50 25 : 75 Forest litter 50 : 50 25 : 75 Manure 25 : 75 10 : 90 Peat (any type) 100 : 0 50 : 50 Rice hulls 50 : 50 25 : 75 Solid digestate 20 : 80 10 : 90 Spent mushroom casing soil 25 : 75 10 : 90 Straw 50 : 50 25 : 75 Wood fibres 50 : 50 25 : 75 Wood chips 50 : 50 25 : 75 Wood shavings 50 : 50 25 : 75 Mineral materials Clay 25 : 75 10 : 90 Expanded clay (also broken) 50 : 50 25 : 75 Lava 50 : 50 25 : 75 Mineral wool flakes 50 : 50 25 : 75 Perlite (expanded) 50 : 50 25 : 75 Pumice 50 : 50 25 : 75 Sand 50 : 50 25 : 75 Vermiculite 50 : 50 25 : 75 Synthetic-organic materials (plastics) Expanded polystyrene flakes (styrofoam), urea-formaldehyde foam resins, etc. 50 : 50 25 : 75 NOTE Non-listed materials may also be tested; the dilution ratio(s) for non-listed materials may be chosen by analogy with similar product groups listed in Table 1. a To obtain the required quantity of test material and sphagnum peat for example at a ratio of 25 % (V/V) : 75 % (V/V), take – for example – one litre of test material and three litres of sphagnum peat. The laboratory compacted bulk density of the materials according to EN 13040 shall be the basis.
5 mm to 10 mm below the rim of the pot. If an even surface is not obtained due to coarse particles, fill spaces with mixed sample material passing through a 5 mm sieve (see 6.2.2). Place each pot on a saucer (see 6.2.4). NOTE 1 A higher number of replicates may be used. The number of replicates should be taken into account for the calculation of the results Spread 20 seeds of Chinese cabbage (see 6.1.5) evenly on the surface of each of three pots of the prepared sample and cover them with a thin layer (approximately 2 mm to 5 mm) of prepared sample passing through a
5 mm sieve (see 6.2.2). Then gently compact the surface again by using a suitable flat, round plate or saucer and moisten with water using a fine sprayer to ensure contact between the seed and the test material. NOTE 2 To cover the Spring barley seeds (see 6.1.6), usually more material (approximately 5 mm to 10 mm) than for cabbage is needed. The pots shall be arranged in the test area in a randomized design to minimise positional effects and bias associated with variations in temperature, light or humidity which affect plant growth.
Then, the pots in the testing facility (see 6.2.6) at a temperature maintained between 18 °C and 30 °C are loosely covered with horticultural fleece or plastic sheet (see 6.2.5) until at least 50 % of the seeds have germinated. After uncovering the pots the light intensity shall be maintained at 10 W m-2 for 12 h to 16 h per day and the sample kept moist by overhead irrigation with water (see 6.1.1). The watering intervals are dependent on plant growth and environmental conditions in accordance with good horticulture practice. Alternatively, a watering process according to ISO 22030 is possible. NOTE 3 Steps should be taken to avoid over-watering and water logging of the test sample. NOTE 4 To improve the watering process, the pots can be weighed during the test to provide an indication of the moisture content of the test samples and guidance about the frequency and quantity of water that needs to be applied to keep them moist. 6.5 Control sample As a control, the procedure as described above is carried out using fertilized and limed sphagnum peat (see 6.1.3). 6.6 Validity of the test If the average germination in the control sample (see 6.5) is below 85 % after five days, the test is not valid. SIST EN 16086-1:2012

50 % of the cotyledon surfac
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