CEN/TS 13130-13:2005
(Main)Materials and articles in contact with foodstuffs - Plastics substances subject to limitation - Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Materials and articles in contact with foodstuffs - Plastics substances subject to limitation - Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants.
NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty food simulants, sunflower oil and a mixture of synthetic triglycerides.
Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Substanzen in Kunststoffen, die Beschränkungen unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
Diese Technische Spezifikation, Teil der EN 13130, legt ein Verfahren zur Bestimmung von Bisphenol A in den Prüflebensmitteln Wasser, 3%ige Essigsäure (m/V) in wässriger Lösung, 15%iges Ethanol (Volumenanteil) in wässriger Lösung und rektifiziertes Olivenöl fest. Der ermittelte Gehalt an monomerem Bisphenol A wird in Milligramm Bisphenol A je Kilogramm Prüflebensmittel angegeben. Das Verfahren eignet sich zur quantitativen Bestimmung von Bisphenol A bei einem Mindestgehalt von 0,2 mg je Kilogramm Prüflebensmittel bis 0,7 mg je Kilogramm Prüflebensmittel.
ANMERKUNG Das Verfahren sollte auch auf andere wässrige Prüflebensmittel sowie auf die anderen fettigen Prüflebensmittel, Sonnenblumenöl und eine Mischung synthetischer Triglyceride anwendbar sein.
Matériaux et objets en contact avec les denrées alimentaires - Substances dans les matières plastiques soumises à des limitations - Partie 13 : Détermination du 2,2-bis(4-hydroxyphènyl) propane (Bisphénol A) dans les simulants d'aliments
Materiali in predmeti v stiku z živili - Snovi v polimernih materialih, katerih koncentracija je omejena - 13. del: Določevanje 2,2-bis(4-hidroksifenil)propana (Bisfenola A) v modelnih raztopinah za živila
General Information
- Status
- Published
- Publication Date
- 22-Feb-2005
- Technical Committee
- CEN/TC 194 - Utensils in contact with food
- Drafting Committee
- CEN/TC 194/SC 1/WG 2 - Methods of test for monomers
- Current Stage
- 6060 - Definitive text made available (DAV) - Publishing
- Start Date
- 23-Feb-2005
- Completion Date
- 23-Feb-2005
Relations
- Effective Date
- 19-Jan-2023
Overview
CEN/TS 13130-13:2005 specifies an analytical method for the determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A, BPA) migrating from plastics into food simulants. It is part of the EN 13130 series addressing materials and articles in contact with foodstuffs and substances in plastics that are subject to limitation. The procedure quantifies BPA in aqueous simulants (water, 3 % w/v acetic acid, 15 % v/v ethanol) and in rectified olive oil, expressing results as mg BPA per kg of food simulant. The method was pre‑validated in a collaborative trial and is intended to support EU food‑contact plastics legislation.
Key topics and technical requirements
- Analytical principle: High-performance liquid chromatography (HPLC) with ultraviolet (UV) detection; diode array detection (DAD) for confirmation.
- Scope of simulants: Water, 3 % acetic acid (w/v), 15 % ethanol (v/v) and rectified olive oil. Note: method is expected to be applicable to other aqueous and fatty simulants (e.g., sunflower oil, synthetic triglyceride mixtures).
- Quantitation range: Method applicable to quantitative determination at a minimum level indicated as 0,2 mg/kg to 0,7 mg/kg of food simulant (as stated in the technical specification).
- Sample preparation:
- Aqueous samples are analyzed directly after appropriate handling.
- Fatty samples (olive oil) are extracted with n‑hexane then methanol/water (1:1) and the lower aqueous/methanolic layer is analyzed.
- Blank and calibration samples are prepared in matching simulants.
- Chromatographic conditions (illustrative example):
- C18 reversed-phase column (e.g., 250 × 4.6 mm, 5 µm), mobile phase methanol/water 70:30, flow ~1 mL/min, detection at ~280 nm.
- Injection of samples in duplicate; detector sensitivity sufficient to detect low nanogram on‑column amounts.
- Calibration and expression: Calibration with matrix-matched standards; results reported as mg BPA per kg simulant (mg/kg).
Applications and users
- Regulatory and compliance testing for migration of BPA from plastic materials and articles intended to contact food.
- Quality control laboratories in the food packaging, plastics and polymer industries.
- National and independent testing laboratories verifying compliance with EU directives on food contact materials.
- Research laboratories studying migration behavior of BPA from polymers.
Related standards
- EN 13130-1: Guide to test methods for specific migration and selection of exposure conditions (to be read in conjunction).
- Other parts of EN 13130 cover determination of specific monomers and migrants (see EN 13130 series).
Keywords: CEN/TS 13130-13:2005, Bisphenol A, BPA, food simulants, HPLC, migration testing, plastics in contact with food, EN 13130, specific migration.
Frequently Asked Questions
CEN/TS 13130-13:2005 is a technical specification published by the European Committee for Standardization (CEN). Its full title is "Materials and articles in contact with foodstuffs - Plastics substances subject to limitation - Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants". This standard covers: This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants. NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty food simulants, sunflower oil and a mixture of synthetic triglycerides.
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants. NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty food simulants, sunflower oil and a mixture of synthetic triglycerides.
CEN/TS 13130-13:2005 is classified under the following ICS (International Classification for Standards) categories: 67.250 - Materials and articles in contact with foodstuffs. The ICS classification helps identify the subject area and facilitates finding related standards.
CEN/TS 13130-13:2005 has the following relationships with other standards: It is inter standard links to prEN 13130-13. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase CEN/TS 13130-13:2005 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2005
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NRQFHQWUDFLMDMHRPHMHQDGHO 'RORþHYDQMHELV KLGURNVLIHQLO SURSDQD
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Materials and articles in contact with foodstuffs - Plastics substances subject to limitation
- Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food
simulants
Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Substanzen in Kunststoffen,
die Beschränkungen unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-Hydroxyphenyl)
Propan (Bisphenol A) in Prüflebensmitteln
Matériaux et objets en contact avec les denrées alimentaires - Substances dans les
matieres plastiques soumises a des limitations - Partie 13 : Détermination du 2,2-bis(4-
hydroxyphenyl) propane (Bisphénol A) dans les simulants d'aliments
Ta slovenski standard je istoveten z: CEN/TS 13130-13:2005
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
TECHNICAL SPECIFICATION
CEN/TS 13130-13
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
February 2005
ICS 67.250
English version
Materials and articles in contact with foodstuffs - Plastics
substances subject to limitation - Part 13: Determination of 2,2-
bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Matériaux et objets en contact avec les denrées Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln
alimentaires - Substances dans les matières plastiques - Substanzen in Kunststoffen, die Beschränkungen
soumises à des limitations - Partie 13 : Détermination du unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-
2,2-bis(4-hydroxyphènyl) propane (Bisphénol A) dans les Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
simulants d'aliments
This Technical Specification (CEN/TS) was approved by CEN on 16 December 2004 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 13130-13:2005: E
worldwide for CEN national Members.
Contents
page
Foreword. 3
Introduction . 5
1 Scope. 6
2 Normative references . 6
3 Principle . 6
4 Reagents . 7
5 Apparatus . 7
6 Samples . 8
7 Procedure . 9
8 Expression of results. 10
9 Confirmation. 11
10 Test report . 12
Bibliography . 13
Foreword
This document (CEN/TS 13130-13:2005) has been prepared by Technical Committee CEN/TC 194
“Utensils in contact with food”, the secretariat of which is held by BSI.
This part of EN 13130 has been prepared within the Standards, Measurement and Testing project,
MAT1-CT92-0006, “Development of Methods of Analysis for Monomers” and has been prepared by
Subcommittee (SC 1) of TC 194 "Utensils in contact with food" as one of a series of test methods for
plastics materials and articles in contact with foodstuffs.
This standard is intended to support Directives 2002/72/EC [1], 89/109/EEC [2], 82/711/EEC [3] and
its amendments 93/8/EEC [4] and 97/48/EC [5], and 85/572/EEC [6].
At the time of preparation and publication of this part of EN 13130 the European Union legislation
relating to plastics materials and articles intended to come into contact with foodstuffs is incomplete.
Further Directives and amendments to existing Directives are expected which could change the
legislative requirements which this standard supports. It is therefore strongly recommended that users
of this standard refer to the latest relevant published Directive(s) before commencement of a test or
tests described in this standard.
This part of EN 13130 should be read in conjunction with EN 13130-1.
Further parts of EN 13130, under the general title Materials and articles in contact with foodstuffs -
Plastics substances subject to limitation, have been prepared, and others are in preparation,
concerned with the determination of specific migration from plastics materials into foodstuffs and food
simulants and the determination of specific monomers and additives in plastics. The parts of
EN 13130 are as follows.
Part 1: Guide to test methods for the specific migration of substances from plastics to foods and food
simulants and the determination of substances in plastics and the selection of conditions of exposure
to food simulants
Part 2: Determination of terephthalic acid in food simulants
Part 3: Determination of acrylonitrile in food and food simulants
Part 4: Determination of 1,3-butadiene in plastics
Part 5: Determination of vinylidene chloride in food simulants
Part 6: Determination of vinylidene chloride in plastics
Part 7: Determination of monoethylene glycol and diethylene glycol in food simulants
Part 8: Determination of isocyanates in plastics
Part 9: Determination of acetic acid, vinyl ester in food simulants
Part 10: Determination of acrylamide in food simulants
Part 11: Determination of 11-aminoundecanoic acid in food simulants
Part 12: Determination of 1,3-benzenedimethanamine in food simulants
Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Part 14: Determination of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indoline in food simulants
Part 15: Determination of 1,3-butadiene in food simulants
Part 16: Determination of caprolactam and caprolactam salt in food simulants
Part 17: Determination of carbonyl chloride in plastics
Part 18: Determination of 1,2-dihydroxybenzene, 1,3-dihydroxybenzene,
1,4-dihydroxybenzene, 4,4’-dihydroxybenzophenone and 4,4’dihydroxybiphenyl in food simulants
Part 19: Determination of dimethylaminoethanol in food simulants
Part 20: Determination of epichlorohydrin in plastics
Part 21: Determination of ethylenediamine and hexamethylenediamine in food simulants
Part 22: Determination of ethylene oxide and propylene oxide in plastics
Part 23: Determination of formaldehyde and hexamethylenetetramine in food simulants
Part 24: Determination of maleic acid and maleic anhydride in food simulants
Part 25: Determination of 4-methyl-pentene in food simulants
Part 26: Determination of 1-octene and tetrahydrofuran in food simulants
Part 27: Determination of 2,4,6-triamino-1,3,5-triazine in food simulants
Part 28: Determination of 1,1,1-trimethylolpropane in food simulants
Parts 1 to 8 are European Standards. Parts 9 to 28 are Technical Specifications.
WARNING All chemicals are hazardous to health to a greater or lesser extent. It is beyond
the scope of this Technical Specification to give instructions for the safe handling of all
chemicals, that meet, in full, the legal obligations in all countries in which this Technical
Specification may be followed. Therefore, specific warnings are not given and users of this
Technical Specification should ensure that they meet all the necessary safety requirements in
their own country.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to announce this CEN Technical Specification: Austria, Belgium, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Introduction
2,2-bis(4-hydroxyphenyl)propane, hereinafter referred to as Bisphenol A, C H O , PM/Ref. 13480, is
15 16 2
a monomer used in the manufacture of certain plastics materials and articles intended to come into
contact with foodstuffs. After manufacture residual Bisphenol A can remain in the finished product and
may migrate into foodstuffs coming into contact with that product.
The method has been pre-validated by a collaborative trial with three laboratories.
1 Scope
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food
simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive
oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per
kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A
at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants.
NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty
food simulants, sunflower oil and a mixture of synthetic triglycerides.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN 13130-1:2004, Materials and articles in contact with foodstuffs – Plastics substances subject to
limitation – Part 1: Guide to test methods for the specific migration of substances from plastics to
foods and food simulants and the determination of substances in plastics and the selection of
conditions of exposure to food simulants.
3 Principle
The level of Bisphenol A in aqueous food simulants is determined by high performance liquid
chromatography (HPLC) with ultra violet (UV) detection. Olive oil test samples are extracted with a
mixture of water/methanol and the resultant solution analyzed by HPLC. Calibration is achieved by
analysis of relevant simulants containing known amounts of Bisphenol A. Confirmation of Bisphenol A
is carried out by diode array detection.
4 Reagents
NOTE All reagents should be of recognized analytical quality unless otherwise stated.
4.1 Analyte
2,2-bis(4-hydroxyphenyl)propane (Bisphenol A or 4,4'-(methylethylidene)-bisphenol or
4,4'-isopropylidenediphenol), C H O , molecular weight: 228,28, purity > 99 %.
15 16 2
4.2 Chemicals
4.2.1 n-Hexane
4.2.2 Methanol
4.2.3 Water, deionized
4.3 Solutions
4.3.1 Extraction solvent, methanol/water = 1:1
Measure 100 ml of methanol (4.2.2) and 100 ml of water (4.2.3) and mix.
4.3.2 Mobile phase for HPLC, methanol/water = 70:30
Measure 500 ml of methanol (4.2.2) and 215 ml of water (4.2.3) and mix.
4.3.3 Stock solution of Bisphenol A in methanol at a defined concentration of approximately
0,38 mg/ml
Weigh to the nearest 0,1 mg approximately 37,5 mg of Bisphenol A (4.1.1) into a 100 ml volumetric
flask. Dissolve the Bisphenol A in methanol and make up to the mark with methanol (4.2.2).
Calculate the concentration in micrograms of Bisphenol A per millilitre of solution.
Repeat the procedure to obtain a second stock solution.
NOTE The solution can be stored in a well closed container in dark for a maximum of 3 months at any
temperature between +20 °C and –20 °C.
5 Apparatus
NOTE An instrument or item of apparatus is listed only where it is special or made to a particular
specification, usual laboratory glassware and equipment being assumed to be available.
5.1 High performance liquid chromatograph, preferably, equipped with an automatic 20 µl loop
injector and a variable wavelength UV detector connected to an integrator.
5.2 HPLC column, capable of separating Bisphenol A fully from peaks originating from the
simulants and/or solvents used.
Appropriate operating conditions shall be established for the specific equipment used for the
determination.
NOTE T
...
SLOVENSKI STANDARD
01-april-2005
Materiali in predmeti v stiku z živili - Snovi v polimernih materialih, katerih
koncentracija je omejena - 13. del: Določevanje 2,2-bis(4-hidroksifenil)propana
(Bisfenola A) v modelnih raztopinah za živila
Materials and articles in contact with foodstuffs - Plastics substances subject to limitation
- Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food
simulants
Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Substanzen in Kunststoffen,
die Beschränkungen unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-
Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
Matériaux et objets en contact avec les denrées alimentaires - Substances dans les
matieres plastiques soumises a des limitations - Partie 13 : Détermination du 2,2-bis(4-
hydroxyphenyl) propane (Bisphénol A) dans les simulants d'aliments
Ta slovenski standard je istoveten z: CEN/TS 13130-13:2005
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
TECHNICAL SPECIFICATION
CEN/TS 13130-13
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
February 2005
ICS 67.250
English version
Materials and articles in contact with foodstuffs - Plastics
substances subject to limitation - Part 13: Determination of 2,2-
bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Matériaux et objets en contact avec les denrées Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln
alimentaires - Substances dans les matières plastiques - Substanzen in Kunststoffen, die Beschränkungen
soumises à des limitations - Partie 13 : Détermination du unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-
2,2-bis(4-hydroxyphènyl) propane (Bisphénol A) dans les Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
simulants d'aliments
This Technical Specification (CEN/TS) was approved by CEN on 16 December 2004 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 13130-13:2005: E
worldwide for CEN national Members.
Contents
page
Foreword. 3
Introduction . 5
1 Scope. 6
2 Normative references . 6
3 Principle . 6
4 Reagents . 7
5 Apparatus . 7
6 Samples . 8
7 Procedure . 9
8 Expression of results. 10
9 Confirmation. 11
10 Test report . 12
Bibliography . 13
Foreword
This document (CEN/TS 13130-13:2005) has been prepared by Technical Committee CEN/TC 194
“Utensils in contact with food”, the secretariat of which is held by BSI.
This part of EN 13130 has been prepared within the Standards, Measurement and Testing project,
MAT1-CT92-0006, “Development of Methods of Analysis for Monomers” and has been prepared by
Subcommittee (SC 1) of TC 194 "Utensils in contact with food" as one of a series of test methods for
plastics materials and articles in contact with foodstuffs.
This standard is intended to support Directives 2002/72/EC [1], 89/109/EEC [2], 82/711/EEC [3] and
its amendments 93/8/EEC [4] and 97/48/EC [5], and 85/572/EEC [6].
At the time of preparation and publication of this part of EN 13130 the European Union legislation
relating to plastics materials and articles intended to come into contact with foodstuffs is incomplete.
Further Directives and amendments to existing Directives are expected which could change the
legislative requirements which this standard supports. It is therefore strongly recommended that users
of this standard refer to the latest relevant published Directive(s) before commencement of a test or
tests described in this standard.
This part of EN 13130 should be read in conjunction with EN 13130-1.
Further parts of EN 13130, under the general title Materials and articles in contact with foodstuffs -
Plastics substances subject to limitation, have been prepared, and others are in preparation,
concerned with the determination of specific migration from plastics materials into foodstuffs and food
simulants and the determination of specific monomers and additives in plastics. The parts of
EN 13130 are as follows.
Part 1: Guide to test methods for the specific migration of substances from plastics to foods and food
simulants and the determination of substances in plastics and the selection of conditions of exposure
to food simulants
Part 2: Determination of terephthalic acid in food simulants
Part 3: Determination of acrylonitrile in food and food simulants
Part 4: Determination of 1,3-butadiene in plastics
Part 5: Determination of vinylidene chloride in food simulants
Part 6: Determination of vinylidene chloride in plastics
Part 7: Determination of monoethylene glycol and diethylene glycol in food simulants
Part 8: Determination of isocyanates in plastics
Part 9: Determination of acetic acid, vinyl ester in food simulants
Part 10: Determination of acrylamide in food simulants
Part 11: Determination of 11-aminoundecanoic acid in food simulants
Part 12: Determination of 1,3-benzenedimethanamine in food simulants
Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Part 14: Determination of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indoline in food simulants
Part 15: Determination of 1,3-butadiene in food simulants
Part 16: Determination of caprolactam and caprolactam salt in food simulants
Part 17: Determination of carbonyl chloride in plastics
Part 18: Determination of 1,2-dihydroxybenzene, 1,3-dihydroxybenzene,
1,4-dihydroxybenzene, 4,4’-dihydroxybenzophenone and 4,4’dihydroxybiphenyl in food simulants
Part 19: Determination of dimethylaminoethanol in food simulants
Part 20: Determination of epichlorohydrin in plastics
Part 21: Determination of ethylenediamine and hexamethylenediamine in food simulants
Part 22: Determination of ethylene oxide and propylene oxide in plastics
Part 23: Determination of formaldehyde and hexamethylenetetramine in food simulants
Part 24: Determination of maleic acid and maleic anhydride in food simulants
Part 25: Determination of 4-methyl-pentene in food simulants
Part 26: Determination of 1-octene and tetrahydrofuran in food simulants
Part 27: Determination of 2,4,6-triamino-1,3,5-triazine in food simulants
Part 28: Determination of 1,1,1-trimethylolpropane in food simulants
Parts 1 to 8 are European Standards. Parts 9 to 28 are Technical Specifications.
WARNING All chemicals are hazardous to health to a greater or lesser extent. It is beyond
the scope of this Technical Specification to give instructions for the safe handling of all
chemicals, that meet, in full, the legal obligations in all countries in which this Technical
Specification may be followed. Therefore, specific warnings are not given and users of this
Technical Specification should ensure that they meet all the necessary safety requirements in
their own country.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to announce this CEN Technical Specification: Austria, Belgium, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Introduction
2,2-bis(4-hydroxyphenyl)propane, hereinafter referred to as Bisphenol A, C H O , PM/Ref. 13480, is
15 16 2
a monomer used in the manufacture of certain plastics materials and articles intended to come into
contact with foodstuffs. After manufacture residual Bisphenol A can remain in the finished product and
may migrate into foodstuffs coming into contact with that product.
The method has been pre-validated by a collaborative trial with three laboratories.
1 Scope
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food
simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive
oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per
kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A
at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants.
NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty
food simulants, sunflower oil and a mixture of synthetic triglycerides.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN 13130-1:2004, Materials and articles in contact with foodstuffs – Plastics substances subject to
limitation – Part 1: Guide to test methods for the specific migration of substances from plastics to
foods and food simulants and the determination of substances in plastics and the selection of
conditions of exposure to food simulants.
3 Principle
The level of Bisphenol A in aqueous food simulants is determined by high performance liquid
chromatography (HPLC) with ultra violet (UV) detection. Olive oil test samples are extracted with a
mixture of water/methanol and the resultant solution analyzed by HPLC. Calibration is achieved by
analysis of relevant simulants containing known amounts of Bisphenol A. Confirmation of Bisphenol A
is carried out by diode array detection.
4 Reagents
NOTE All reagents should be of recognized analytical quality unless otherwise stated.
4.1 Analyte
2,2-bis(4-hydroxyphenyl)propane (Bisphenol A or 4,4'-(methylethylidene)-bisphenol or
4,4'-isopropylidenediphenol), C H O , molecular weight: 228,28, purity > 99 %.
15 16 2
4.2 Chemicals
4.2.1 n-Hexane
4.2.2 Methanol
4.2.3 Water, deionized
4.3 Solutions
4.3.1 Extraction solvent, methanol/water = 1:1
Measure 100 ml of methanol (4.2.2) and 100 ml of water (4.2.3) and mix.
4.3.2 Mobile phase for HPLC, methanol/water = 70:30
Measure 500 ml of methanol (4.2.2) and 215 ml of water (4.2.3) and mix.
4.3.3 Stock solution of Bisphenol A in methanol at a defined concentration of approximately
0,38 mg/ml
Weigh to the nearest 0,1 mg approximately 37,5 mg of Bisphenol A (4.1.1) into a 100 ml volumetric
flask. Dissolve the Bisphenol A in methanol and make up to the mark with methanol (4.2.2).
Calculate the concentration in micrograms of Bisphenol A per millilitre of solution.
Repeat the procedure to obtain a second stock solution.
NOTE The solution can be stored in a well closed container in dark for a maximum of 3 months at any
temperature between +20 °C and –20 °C.
5 Apparatus
NOTE An instrument or item of apparatus is listed only where it is special or made to a particular
specification, usual laboratory glassware and equipment being assumed to be available.
5.1 High performance liquid chromatograph, preferably, equipped with an automatic 20 µl loop
injector and a variable wavelength UV detector connected to an integrator.
5.2 HPLC column, capable of separating Bisphenol A fully from peaks originating from the
simulants and/or solvents used.
Appropriate operating conditions shall be established for the specific equipment used for the
determination.
NOTE The
...
The article discusses a document, CEN/TS 13130-13:2005, which outlines a method for determining the presence of Bisphenol A (a harmful plastic substance) in different food simulants. The method can be used to measure the Bisphenol A content in water, acetic acid aqueous solution, ethanol aqueous solution, and rectified olive oil. The results are expressed in milligrams per kilogram of food simulant. The method can detect Bisphenol A at levels ranging from 0.2 mg/kg to 0.7 mg/kg in food simulants, and it may also be applicable to other aqueous and fatty food simulants like sunflower oil and synthetic triglyceride mixtures.
記事タイトル：CEN/TS 13130-13:2005 - 食品と接触する材料 - 制限を受けるプラスチック物質 - 第13部：食品模擬体中の2,2-ビス(4-ヒドロキシフェニル)プロパン（ビスフェノールA）の測定 記事内容：この文書はEN 13130の一部であり、食品模擬体である水、3 % w/v 酢酸水溶液、15 % v/v エタノール水溶液、および脱臭オリーブオイル中のビスフェノールAの測定方法を規定しています。測定されたビスフェノールAモノマーのレベルは食品模擬体1キログラムあたりのミリグラムビスフェノールAとして表されます。この方法は、食品模擬体中のビスフェノールAを0.2 mg/kgから0.7 mg/kgの最小レベルで定量的に測定するのに適用されます。 注意：この方法は、他の水性食品模擬体や他の脂肪性食品模擬体であるひまわり油や合成トリグリセリド混合物にも適用可能です。
기사 제목: CEN/TS 13130-13:2005 - 식품 접촉용 재료 및 제품 - 제한되는 플라스틱 물질 - 제 13부: 식품 시뮬린트에서 2,2-비스(4-히드록시페닐)프로판 (비스페놀 A)의 정량화 방법 기사 내용: 이 문서는 EN 13130의 일부인 CEN/TS 13130-13:2005로, 식품 시뮬린트인 물, 3% w/v 요산 수용액, 15% v/v 에탄올 수용액, 정제된 올리브 오일에서 비스페놀 A의 양을 정량화하는 방법을 명시하고 있다. 측정된 비스페놀 A 단량체의 수준은 식품 시뮬린트 1kg당 밀리그램 단위로 표시된다. 이 방법은 식품 시뮬린트 내의 비스페놀 A를 0.2mg/kg에서 0.7mg/kg 최소 수준에서 정량화할 수 있다. 이 방법은 다른 수분이 많은 식품 시뮬린트 및 해바라기 오일 및 합성 트리글리세라이드 혼합물과 같은 다른 지방성 식품 시뮬린트에도 적용할 수 있다.
기사 제목: CEN / TS 13130-13:2005 - 식품과 접촉할 수있는 재료 및 제품 - 제한을 받는 플라스틱 물질 - 파트 13 : 식품 유사체에서 2,2-비스 (4-하이드록시페닐)프로판(비스페놀 A)의 결정 기사 내용: 이 문서는 EN 13130의 일부로, 식품 유사체인 물, 3 % w/v 아세트산 수용액, 15 % v/v 에탄올 수용액, 정제된 올리브 오일에서의 비스페놀 A의 결정을 위한 방법을 규정합니다. 결정된 비스페놀 A 단량체의 수준은 식품 유사체당 밀리그램 단위로 표현됩니다. 이 방법은 0.2 mg/kg에서 0.7 mg/kg의 최소 수준에서 비스페놀 A의 정량 결정에 적용될 수 있습니다. 참고: 이 방법은 다른 수용성 식품 유사체뿐만 아니라 태양꽃오일과 합성 트리글리세이드 혼합물과 같은 다른 지방성 식품 유사체에도 적용될 수 있습니다.
The article discusses the CEN/TS 13130-13:2005 standard, which focuses on the determination of Bisphenol A (BPA) in various food simulants. The standard specifies a method for measuring BPA levels in water, acetic acid aqueous solution, ethanol aqueous solution, and rectified olive oil. The BPA concentration is expressed in milligrams per kilogram of food simulant. The method can be used to quantify BPA levels ranging from 0.2 mg/kg to 0.7 mg/kg in food simulants. The method may also be applicable to other types of aqueous and fatty food simulants, such as sunflower oil and synthetic triglyceride mixtures.
記事のタイトル: CEN/TS 13130-13:2005 - 食品と接触する材料および製品 - 制約されるプラスチック物質 - 第13部: 食品シミュラント中の2,2-ビス(4-ヒドロキシフェニル)プロパン (ビスフェノールA)の定量方法 記事の内容: この文書はEN 13130の一部であるCEN/TS 13130-13:2005に関して、食品シミュラントである水、3% w/v酢酸水溶液、15% v/vエタノール水溶液、精製オリーブオイル中のビスフェノールAの存在を定量化する方法を規定しています。測定結果は食品シミュラント1kgあたりのミリグラム単位で表示されます。この方法は食品シミュラント中のビスフェノールAを0.2mg/kgから0.7mg/kgの最小レベルで定量化することが可能です。この方法は他の水系の食品シミュラントだけでなく、向日葵油や合成トリグリセリドの混合物などの脂肪食品シミュラントにも適用可能です。
記事のタイトル：CEN/TS 13130-13:2005 - 食品と接触する材料および製品 - 制約対象となるプラスチック物質 - 第13部：食品模擬物中の2,2-ビス（4-ヒドロキシフェニル）プロパン（ビスフェノールA）の測定 記事の内容：この文書は、EN 13130の一部であり、水、3％w/vの酢酸水溶液、15％v/vのエタノール水溶液、精製オリーブオイルの食品模擬物中のビスフェノールAを測定する方法を規定しています。測定されたビスフェノールA単量体のレベルは、食品模擬物1キログラム当たりのミリグラムとして表されます。この方法は、食品模擬物中のビスフェノールAを0.2 mg/kgから0.7 mg/kgの最低レベルで定量的に測定するのに適用できます。 注意：この方法は、他の水性食品模擬物だけでなく、ひまわり油や合成トリグリセリドの混合物などの他の脂肪性食品模擬物にも適用できる可能性があります。
The article is about CEN/TS 13130-13:2005, which is part of EN 13130 and specifies a method for determining the presence of Bisphenol A (BPA) in food simulants. The method can be used to measure BPA in water, 3% acetic acid aqueous solution, 15% ethanol aqueous solution, and rectified olive oil. The results are expressed as milligrams of BPA per kilogram of food simulant. The method can detect BPA at levels ranging from 0.2 mg/kg to 0.7 mg/kg in food simulants. This method can also be adapted for other aqueous food simulants as well as fatty food simulants like sunflower oil and a mixture of synthetic triglycerides.
CEN/TS 13130-13:2005 - 식품과 접촉하는 자재 및 용기 - 제한을 받는 플라스틱 물질 - 13부: 식품 시뮬란트에서 2,2-비스(4-히드록시페닐)프로판 (비스페놀 A)의 측정 이 문서는 EN 13130의 일부로, 식품 시뮬란트인 물, 3 % acetic acid 수용액, 15 % 에탄올 수용액 및 정제된 올리브 오일에서 비스페놀 A를 측정하는 방법을 명시한다. 측정된 비스페놀 A 모노머의 수치는 식품 시뮬란트 1 킬로그램 당 밀리그램 비스페놀 A로 표시된다. 이 방법은 최소 0.2 mg/kg에서 0.7 mg/kg의 비스페놀 A 함량을 식품 시뮬란트에서 양적으로 측정하는 데 적용 가능하다. 참고: 이 방법은 그 외의 수분 식품 시뮬란트와 해바라기 오일 및 합성 트리글리세라이드 혼합물과 같은 기름질 식품 시뮬란트에도 적용 가능해야 한다.
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