EN 15779:2009+A1:2013
(Main)Petroleum products and fat and oil derivates - Fatty acid methyl esters (FAME) for diesel engines - Determination of polyunsaturated (≥4 double bonds) fatty acid methyl esters (PUFA) by gas chromatography
Petroleum products and fat and oil derivates - Fatty acid methyl esters (FAME) for diesel engines - Determination of polyunsaturated (≥4 double bonds) fatty acid methyl esters (PUFA) by gas chromatography
This European Standard specifies a method for the determination of the polyunsaturated (≥ 4 double bonds) fatty acid (PUFA) methyl esters content of fatty acid methyl ester (FAME) as a whole between 0,6 % (m/m) and 1,5 % (m/m).
The method covers the predominant four polyunsaturated fatty acid methyl esters of eicosatetraenoic acid (C 20:4 (n-6)), eicosapentaenoic acid (C 20:5 (n-3)), docosapentaenoic acid (C 22:5 (n 3), and docosahexaenoic acid (C 22:6 (n 3)).
Studies have indicated that based on the linearity of results from this European Standard, PUFA methyl esters can be determined in FAME in the range between 0,3 % (m/m) to 3,0 % (m/m). However, the precision was not established in that range, as no samples within the upper ranges where included in the final interlaboratory test (see 10.1).
Although the method is applicable to all uses, it is predominantly for FAME for use in diesel engines.
NOTE 1 For the purposes of this document, the term “% (m/m)” is used to represent the mass fraction of a material.
NOTE 2 This European Standard is based on A.O.C.S Official Method Ce 1b-89 [1].
Mineralölerzeugnisse und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester (FAME) für Dieselmotoren - Bestimmung von mehrfach ungesättigten (≥ 4 Doppelbindungen) Fettsäuremethylestern (PUFA) mittels Gaschromatographie
Diese Europäische Norm legt ein Prüfverfahren für die Bestimmung des Gehaltes an mehrfach ungesättigten ( 4 Doppelbindungen) Fettsäuremethylestern (en: polyunsaturated fatty acid methyl ester, PUFA methyl ester) in Fettsäuremethylester (FAME) im Bereich von 0,6 % (m/m) bis 1,5 % (m/m) fest.
Das Prüfverfahren umfasst die Bestimmung der vier vorherrschenden mehrfach ungesättigten Fettsäure-methylester Eicosatetraensäure (C 20:4 (n-6)), Eicosapentaensäure (C 20:5 (n-3)), Docosapentaensäure (C 22:5 (n 3)) und Docosahexaensäure (C 22:6 (n 3)).
Studien haben gezeigt, dass basierend auf der Linearität von Ergebnissen nach dieser Europäischen Norm, PUFA-Methylester in FAME in einem Bereich von 0,3 % (m/m) bis 3,0 % (m/m) bestimmt werden können. Da im abschließenden Ringversuch keine Proben aus dem oberen Konzentrationsbereich einbezogen worden waren, ist die Präzision für diesen Bereich nicht bestimmt worden (siehe 10.1).
Obwohl dieses Prüfverfahren allgemein eingesetzt werden kann, gilt es hauptsächlich für die Untersuchung von FAME für den Einsatz in Dieselmotoren.
ANMERKUNG 1 Für die Zwecke dieses Dokuments wird das Symbol % (m/m) verwendet, um Massenanteile von Materialien in % auszudrücken.
ANMERKUNG 2 Diese Europäische Norm wurde auf der Basis von A.O.C.S. Official Method Ce 1b-98 [1] erstellt.
Produits pétroliers et produits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) pour moteurs diesel (gazole) - Détermination de la teneur en esters méthyliques d'acides gras polyinsaturés (≥ 4 doubles liaisons) (PUFA) par chromatographie en phase gazeuse
La présente Norme européenne prescrit une méthode pour déterminer la teneur en esters méthyliques d'acides gras polyinsaturés ( 4 doubles liaisons) (PUFA) des esters méthyliques d'acides gras (EMAG) en totalité, entre 0,6 % (m/m) et 1,5 % (m/m).
La méthode couvre les quatre principaux esters méthyliques d'acides gras polyinsaturés des acides éicosatétraénoïque (C 20:4 (n-6)), éicosapentaénoïque (C 20:5 (n-3)), docosapentaénoïque (C 22:5 (n-3)) et docosahexaénoïque (C 22:6 (n-3)).
Les études ont montré, à partir de la linéarité des résultats obtenus suivant la présente Norme européenne, que la teneur en esters méthyliques des PUFA peut être déterminée dans les EMAG quand celle-ci est comprise entre 0,3 % (m/m) et 3,0 % (m/m). Cependant, les valeurs de fidélité n’ont pas été établies dans ce domaine, étant donné qu’il n’y avait aucun échantillon dans la fourchette supérieure lors du dernier essai interlaboratoires (voir 10.1).
Bien que cette méthode soit valable pour toutes les applications, elle est principalement destinée aux EMAG utilisés dans les moteurs diesel.
NOTE 1 Pour les besoins de ce document, le terme «% (m/m)» est utilisé pour représenter la fraction massique des produits.
NOTE 2 La présente Norme européenne est basée sur la Méthode Officielle A.O.C.S Ce 1b-89 [1].
Naftni proizvodi ter maščobni in oljni derivati - Metilni estri maščobnih kislin (FAME) za dizelske motorje - Določevanje polinenasičenih (≥ 4 dvojne vezi) metilnih estrov maščobnih kislin (PUFA) s plinsko kromatografijo
Ta evropski standard določa metodo za določanje polinenasičenih (≥ 4 dvojne vezi) metilnih estrov maščobnih kislin (PUFA) v metilnih estrih maščobnih kislin (FAME) v količini med 0,6 % (m/m) in 1,5 % (m/m). Ta metoda zajema štiri glavne polinenasičene metilne estre maščobnih kislin eikozatetraenojske kisline (C 20:4 (n-6)), eikozapentanojske kisline (C 20:5 (n-3)), dokozapentanojske kisline (C22:5 (n-3) in dokozaheksanojske kisline (C 22:6 (n-3)). Študije so na podlagi linearnih rezultatov tega evropskega standarda pokazale, da so polinenasičeni metilni estri maščobnih kislin v metilnih estrih maščobnih kislin zaznavni v območju med 0,3 % (m/m) do 3,0 (m/m). Toda v tem območju ni bila dosežena natančnost, ker v medlaboratorijske preiskave niso bili vključeni nobeni vzorci v zgornjih mejah (glej 10.1). Čeprav se ta metoda uporablja za vse namene, se uporablja pretežno za določanje polinenasičenih maščobnih kislin za uporabo v dizelskih motorjih.
General Information
- Status
- Published
- Publication Date
- 24-Sep-2013
- Withdrawal Date
- 30-Mar-2014
- Technical Committee
- CEN/TC 19 - Petroleum products, lubricants and related products
- Current Stage
- 9093 - Decision to confirm - Review Enquiry
- Start Date
- 09-Dec-2020
- Completion Date
- 23-Sep-2025
Relations
- Effective Date
- 24-Jul-2013
Overview
EN 15779:2009+A1:2013 (CEN) defines a gas chromatography (GC) method for determining the content of polyunsaturated fatty acid methyl esters (PUFA, ≥4 double bonds) in fatty acid methyl ester (FAME) fuels (biodiesel), primarily for diesel-engine use. The method quantifies the combined mass fraction of the predominant PUFA methyl esters - C20:4 (n‑6), C20:5 (n‑3), C22:5 (n‑3) and C22:6 (n‑3) - in the range 0.6 % (m/m) to 1.5 % (m/m). Linearity has been shown between 0.3 % and 3.0 % (m/m), but precision was established only in the 0.6–1.5 % range.
Key topics and requirements
- Analytical principle: GC with flame ionization detector (FID) using an internal standard (C23:0 methyl ester) and theoretical detector correction factors for each PUFA.
- Target analytes: PUFA methyl esters with ≥4 double bonds - C20:4, C20:5, C22:5, C22:6.
- Typical procedure:
- Prepare internal standard (≈25 mg C23:0 in 25 mL heptane).
- Weigh ~100 mg FAME, add 1 mL IS, inject 1 µL into GC.
- Chromatographic conditions (examples from method):
- Capillary column: 30 m, 0.20–0.32 mm i.d., 0.25 µm film; bonded Carbowax / polyethylene glycol phase (e.g., HP‑INNOWax, SUPELCOWAX 10).
- Injector ≈220 °C, detector ≈275 °C, split ≈1:50; temperature program starting 150 °C with ramps to 250 °C.
- Carrier gas: hydrogen or helium (≥99.99 %).
- Calculation: PUFA content calculated using peak areas, internal standard concentration/volume, sample mass and specific correction factors (20:4 → 0.99; 20:5 → 0.98; 22:5 → 0.98; 22:6 → 0.97).
- Reporting: Mass percentage expressed in % (m/m), rounded to 0.01.
- Precision: repeatability r = 0.07 % (m/m); reproducibility R = 0.27 % (m/m).
Applications and users
- Quality control and conformity testing of biodiesel (FAME) for diesel engines to ensure PUFA content limits that affect oxidation stability.
- Test and analytical laboratories performing fuel specification compliance testing.
- Biodiesel producers and blend formulators monitoring oxidative stability risks related to high‑PUFA feedstocks (e.g., marine oils).
- Regulators and certification bodies setting or enforcing fuel quality limits.
Related standards
- EN ISO 3170 / EN ISO 3171 - sampling of petroleum liquids
- EN ISO 5509 - preparation of fatty acid methyl esters (marine oils sample prep)
- EN ISO 4259 - precision data and statistical evaluation
- A.O.C.S. Official Method Ce 1b‑89 - source method on which EN 15779 is based
Keywords: EN 15779, FAME, PUFA, gas chromatography, GC‑FID, biodiesel, fatty acid methyl esters, diesel engines, C20:5, C22:6, fuel testing.
Frequently Asked Questions
EN 15779:2009+A1:2013 is a standard published by the European Committee for Standardization (CEN). Its full title is "Petroleum products and fat and oil derivates - Fatty acid methyl esters (FAME) for diesel engines - Determination of polyunsaturated (≥4 double bonds) fatty acid methyl esters (PUFA) by gas chromatography". This standard covers: This European Standard specifies a method for the determination of the polyunsaturated (≥ 4 double bonds) fatty acid (PUFA) methyl esters content of fatty acid methyl ester (FAME) as a whole between 0,6 % (m/m) and 1,5 % (m/m). The method covers the predominant four polyunsaturated fatty acid methyl esters of eicosatetraenoic acid (C 20:4 (n-6)), eicosapentaenoic acid (C 20:5 (n-3)), docosapentaenoic acid (C 22:5 (n 3), and docosahexaenoic acid (C 22:6 (n 3)). Studies have indicated that based on the linearity of results from this European Standard, PUFA methyl esters can be determined in FAME in the range between 0,3 % (m/m) to 3,0 % (m/m). However, the precision was not established in that range, as no samples within the upper ranges where included in the final interlaboratory test (see 10.1). Although the method is applicable to all uses, it is predominantly for FAME for use in diesel engines. NOTE 1 For the purposes of this document, the term “% (m/m)” is used to represent the mass fraction of a material. NOTE 2 This European Standard is based on A.O.C.S Official Method Ce 1b-89 [1].
This European Standard specifies a method for the determination of the polyunsaturated (≥ 4 double bonds) fatty acid (PUFA) methyl esters content of fatty acid methyl ester (FAME) as a whole between 0,6 % (m/m) and 1,5 % (m/m). The method covers the predominant four polyunsaturated fatty acid methyl esters of eicosatetraenoic acid (C 20:4 (n-6)), eicosapentaenoic acid (C 20:5 (n-3)), docosapentaenoic acid (C 22:5 (n 3), and docosahexaenoic acid (C 22:6 (n 3)). Studies have indicated that based on the linearity of results from this European Standard, PUFA methyl esters can be determined in FAME in the range between 0,3 % (m/m) to 3,0 % (m/m). However, the precision was not established in that range, as no samples within the upper ranges where included in the final interlaboratory test (see 10.1). Although the method is applicable to all uses, it is predominantly for FAME for use in diesel engines. NOTE 1 For the purposes of this document, the term “% (m/m)” is used to represent the mass fraction of a material. NOTE 2 This European Standard is based on A.O.C.S Official Method Ce 1b-89 [1].
EN 15779:2009+A1:2013 is classified under the following ICS (International Classification for Standards) categories: 75.160.20 - Liquid fuels; 75.160.40 - Biofuels. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 15779:2009+A1:2013 has the following relationships with other standards: It is inter standard links to EN 15779:2009. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.PHKUIDFKPLWWHOV*DVFKURPDWRJUDILHJD]HXVH75.160.20Liquid fuelsICS:Ta slovenski standard je istoveten z:EN 15779:2009+A1:2013SIST EN 15779:2009+A1:2013en,fr,de01-december-2013SIST EN 15779:2009+A1:2013SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15779:2009+A1
September 2013 ICS 75.160.20 Supersedes EN 15779:2009
English Version
Petroleum products and fat and oil derivates - Fatty acid methyl esters (FAME) for diesel engines - Determination of polyunsaturated (≥4 double bonds) fatty acid methyl esters (PUFA) by gas chromatography
Produits pétroliers et produits dérivés des corps gras - Esters méthyliques d'acides gras (EMAG) pour moteurs diesel (gazole) - Détermination de la teneur en esters méthyäiques daacides gras éoäyinsaturés (≥ 4 doubäes liaisons) (PUFA) par chromatographie en phase gazeuse
Mineralölerzeugnisse und Erzeugnisse aus pflanzlichen und tierischen Fetten und Ölen - Fettsäure-Methylester (FAME) für Dieselmotoren - Bestimmung von mehrfach ungesättigten (≥ 4 aoééeäbindungen) Fettsäuremethylestern (PUFA) mittels Gaschromatographie This European Standard was approved
by CEN on 22 September 2009 and includes Amendment 1 approved by CEN on 5 August 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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B-1000 Brussels © 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15779:2009+A1:2013: E SIST EN 15779:2009+A1:2013
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