EN ISO 648:2008

SLOVENSKI STANDARD
01-december-2008
1DGRPHãþD
SIST ISO 648:1995
Laboratorijska steklovina - Pipete z eno oznako volumna (ISO 648:2008)
Laboratory glassware - Single volume pipettes (ISO 648:2008)
Laborgeräte aus Glas - Vollpipetten (ISO 648:2008)
Verrerie de laboratoire - Pipettes à un volume (ISO 648:2008)
Ta slovenski standard je istoveten z: EN ISO 648:2008
ICS:
17.060 Merjenje prostornine, mase, Measurement of volume,
gostote, viskoznosti mass, density, viscosity
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 648
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2008
ICS 17.060
English Version
Laboratory glassware - Single-volume pipettes (ISO 648:2008)
Verrerie de laboratoire - Pipettes à un volume (ISO Laborgeräte aus Glas - Vollpipetten (ISO 648:2008)
648:2008)
This European Standard was approved by CEN on 4 October 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 648:2008: E
worldwide for CEN national Members.

Contents Page
Foreword.3

Foreword
This document (EN ISO 648:2008) has been prepared by Technical Committee ISO/TC 48 "Laboratory
glassware and related apparatus" in collaboration with Technical Committee CEN/TC 332 “Laboratory
equipment”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by April 2009, and conflicting national standards shall be withdrawn at the
latest by April 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 648:2008 has been approved by CEN as a EN ISO 648:2008 without any modification.

INTERNATIONAL ISO
STANDARD 648
Second edition
2008-10-15
Laboratory glassware — Single-volume
pipettes
Verrerie de laboratoire — Pipettes à un volume

Reference number
ISO 648:2008(E)
©
ISO 2008
ISO 648:2008(E)
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ii © ISO 2008 – All rights reserved

ISO 648:2008(E)
Contents Page
Foreword. iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Basis of adjustment. 2
4.1 Unit of volume. 2
4.2 Delivery volume . 2
4.3 Reference temperature. 2
5 Types and classes of accuracy. 2
5.1 Classes of accuracy . 2
5.2 Types of single-volume pipette . 2
6 Maximum permissible errors. 3
7 Construction. 3
7.1 Material . 3
7.2 Shape . 3
7.3 Bulb . 3
7.4 Dimensions. 4
7.5 Top of pipette . 5
7.6 Delivery jet. 5
7.7 Delivery time. 5
7.8 Waiting time. 6
8 Graduation line(s) . 6
9 Setting of the meniscus . 6
10 Marking . 6
11 Visibility of graduation lines, figures and inscriptions. 7
12 Colour coding. 7
Bibliography . 8

ISO 648:2008(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 648 was prepared by Technical Committee ISO/TC 48, Laboratory equipment, Subcommittee SC 6,
Laboratory and volumetric ware.
This second edition cancels and replaces the first edition (ISO 648:1977), which has been technically revised
to incorporate the following modifications:
a) single-volume pipettes with two marks have been added;
b) the waiting time for pipettes Class AS has been reduced to 5 s;
c) dimensions have been modified;
d) the nominal volume of 200 ml has been deleted.

iv © ISO 2008 – All rights reserved

INTERNATIONAL STANDARD ISO 648:2008(E)

Laboratory glassware — Single-volume pipettes
1 Scope
This International Standard specifies metrological and constructional requirements for volumetric pipettes with
one mark (total delivery) and for volumetric pipettes with two marks, both of which are adequate for general
laboratory purposes.
The details specified are in conformity with the principles of design and construction of volumetric glassware
given in ISO 384.
NOTE For graduated pipettes, see ISO 835. For piston-operated pipettes, see ISO 8655-2.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 384:1978, Laboratory glassware — Principles of design and construction of volumetric glassware
ISO 719, Glass — Hydrolytic resistance of glass grains at 98 °C — Method of test and classification
ISO 1769, Laboratory glassware — Pipettes — Colour coding
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
delivery volume
volume of liquid discharged from the pipette
NOTE Due to retention of liquid on the inner surface of the volumetric instrument, the volume of liquid delivered is not
identical with the volume of liquid contained by the volumetric instrument.
3.2
delivery time
time required for the descent of the liquid meniscus from the graduation line to the point at which the meniscus
appears to come to rest in the jet for one-mark pipettes and from the upper graduation line to the lower
graduation line for two-mark pipettes
3.3
waiting time
time to be observed after apparent completion of the liquid delivery of the volumetric instrument
NOTE A waiting time applies for Class AS single-volume pipettes (see Clause 5 and 7.8).
ISO 648:2008(E)
4 Basis of adjustment
4.1 Unit of volume
The unit of volume shall be the millilitre (ml), which is equivalent to one cubic centimetre (cm ).
4.2 Delivery volume
Single-volume pipettes shall be adjusted with water of grade 3 quality, in accordance with to ISO 3696, to
deliver their nominal volume (Ex). The delivery should be
...