EN ISO 8655-2:2002

SLOVENSKI STANDARD
01-november-2002
9ROXPHWULþQHQDSUDYHGHOXMRþHQDEDWGHO%DWQHSLSHWH,62
Piston-operated volumetric apparatus - Part 2: Piston pipettes (ISO 8655-2:2002)
Volumenmessgeräte mit Hubkolben - Teil 2: Kolbenhubpipetten (ISO 8655-2:2002)
Appareils volumétriques a piston - Partie 2: Pipettes a piston (ISO 8655-2:2002)
Ta slovenski standard je istoveten z: EN ISO 8655-2:2002
ICS:
17.060 Merjenje prostornine, mase, Measurement of volume,
gostote, viskoznosti mass, density, viscosity
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 8655-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2002
ICS 17.060
English version
Piston-operated volumetric apparatus - Part 2: Piston pipettes
(ISO 8655-2:2002)
Appareils volumétriques à piston - Partie 2: Pipettes à Volumenmessgeräte mit Hubkolben - Teil 2:
piston (ISO 8655-2:2002) Kolbenhubpipetten (ISO 8655-2:2002)
This European Standard was approved by CEN on 13 July 2002.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2002 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8655-2:2002 E
worldwide for CEN national Members.

Foreword
This document (ISO 8655-2:2002) has been prepared by Technical Committee ISO/TC 48
"Laboratory glassware and related apparatus" in collaboration with Technical Committee
CEN/TC 332 "Laboratory equipment", the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by March 2003, and conflicting national
standards shall be withdrawn at the latest by March 2003.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the
United Kingdom.
Endorsement notice
The text of the International Standard ISO 8655-2:2002 has been approved by CEN as a
European Standard without any modifications.
NOTE Normative references to International Standards are listed in Annex ZA (normative).
Annex ZA (normative)
Normative references to international publications with their relevant European
publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and
the publications are listed hereafter. For dated references, subsequent amendments to or
revisions of any of these publications apply to this European Standard only when incorporated
in it by amendment or revision. For undated references the latest edition of the publication
referred to applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
EN ISO 3696 1995 Water for analytical laboratory use - ISO 3696 1987
Specification and test methods
INTERNATIONAL ISO
STANDARD 8655-2
First edition
2002-09-15
Piston-operated volumetric apparatus —
Part 2:
Piston pipettes
Appareils volumétriques à piston —
Partie 2: Pipettes à piston
Reference number
ISO 8655-2:2002(E)
ISO 8655-2:2002(E)
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©
ii ISO 2002 – All rights reserved

ISO 8655-2:2002(E)
Contents Page
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle of operation . 2
5 Design . 2
6 Type, designation . 5
7 Metrological performance requirements . 5
8 Marking . 8
Annexes
A Information to be provided by the supplier for the user of piston pipettes and tips . 9
B Possible sources of error for piston pipettes with air interface . 10
Bibliography. 11
©
ISO 2002 – All rights reserved iii

ISO 8655-2:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this part of ISO 8655 may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 8655-2 was prepared by Technical Committee ISO/TC 48, Laboratory glassware and
related apparatus, Subcommittee SC 1, Volumetric instruments.
ISO 8655 consists of the following parts, under the general title Piston-operated volumetric apparatus:
— Part 1: Terminology, general requirements and user recommendations
— Part 2: Piston pipettes
— Part 3: Piston burettes
— Part 4: Dilutors
— Part 5: Dispensers
— Part 6: Gravimetric methods for the determination of measurement error
The following part is under preparation:
— Part 7: Non-gravimetric methods for the determination of measurement error
Annex A forms a normative part of this part of ISO 8655. Annex B is for information only.
©
iv ISO 2002 – All rights reserved

ISO 8655-2:2002(E)
Introduction
ISO 8655 addresses the needs of:
— suppliers, as a basis for quality control including, where appropriate, the issuance of supplier's declarations;
— test houses and other bodies, as a basis for independent certification;
— users of the equipment, to enable routine checking of accuracy.
The tests specified should be carried out by trained personnel.
©
ISO 2002 – All rights reserved v

INTERNATIONAL STANDARD ISO 8655-2:2002(E)
Piston-operated volumetric apparatus —
Part 2:
Piston pipettes
1 Scope
This part of ISO 8655 specifies
— metrological requirements,
— maximum permissible errors,
— requirements for marking and
— information to be provided for users,
for air-displacement (type A) and positive-displacement (type D) single-channel and multi-channel piston pipettes,
complete with their selected tip(s) and any other essential, consumable parts, designed to deliver their specified
nominal volume (Ex).
NOTE General requirements and definitions of terms of piston-operated volumetric apparatus are given in ISO8655-1.
Conformity testing (type evaluation) of piston-operated volumetric apparatus is given in ISO 8655-6. Alternative test methods such
as photometric and titrimetric methods will be the subject of a future Part 7 to ISO 8655. For all other tests (e.g. quality assurance
by the supplier, analytical and measuring equipment quality assurance by the user) see ISO 8655-6 or alternative test methods.
For safety requirements of electrically powered piston pipettes, see IEC 61010-1.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of
this part of ISO 8655. For dated references, subsequent amendments to, or revisions of, any of these publications do
not apply. However, parties to agreements based on this part of ISO 8655 are encouraged to investigate the
possibility of applying the most recent editions of the normative documents indicated below. For undated references,
the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of
currently valid International Standards.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 8655-1:2002, Piston-operated volumetric apparatus — Part 1: Terminology, general requirements and user
recommendations
ISO 8655-6:2002, Piston-operated volumetric apparatus — Part 6: Gravimetric methods for the determination of
measurement error
©
ISO 2002 – All rights reserved 1

ISO 8655-2:2002(E)
3 Terms and definitions
For the purposes of this part of ISO 8655, the terms and definitions given in ISO 8655-1 and the following apply.
3.1
nominal volume
〈variable-volume piston pipette〉 greatest volume selectable by the user and specified by the manufacturer
NOTE This means that a variable-volume piston pipette with a useful volume range (see ISO 8655-1) of 10µl to 100µl has the
nominal volume of 100µl.
3.2
nominal volume
〈multi-channel piston pipette〉 greatest volume selectable by the user and specified by the manufacturer per channel
4 Principle of operation
The tip made of plastic or glass is attached to the piston pipette. With the piston at the lower aspiration limit, the tip is
dipped into the liquid to be dispensed as a measured volume. When moved to the upper aspiration limit, the piston
aspirates the liquid. The liquid volume to be dispensed is then expelled by depressing or sliding the piston between
the volume-defining limits. Some air-displacement piston pipettes (see 5.1, type A) have an extra air volume which
can be used to expel the last drop of liquid.
See also Figure 1.
5Design
5.1 Types of piston pipette
A piston pipette may be designed as follows:
— fixed volume, designed by the manufacturer to dispense only its nominal volume, for example 100µl;
— variable volume, designed by the manufacturer to dispense volumes selectable by the user within its specified
useful volume range, for example between 10µl and 100µl.
The
...