EN ISO 20418-3:2020
(Main)Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)
Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)
This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.
NOTE 1 The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition.
The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.
NOTE 2 In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts)].
Textilien - Qualitative und quantitative Proteomanalyse einiger Tierhaarfasern - Teil 3: Peptiddetektion mit LC-MS ohne Proteinreduktion (ISO 20418-3:2020)
Dieses Dokument legt ein qualitatives und quantitatives Verfahren zur Bestimmung der Zusammensetzung von Tierhaarfasermischungen (aus Wolle, Kaschmir, Yak, Alpaka, Kamel oder Angora) mittels LC MS ohne Proteinreduktion fest.
ANMERKUNG 1 Die Zusammensetzung von Haarfasern nichttierischen Ursprungs kann mithilfe von ISO 1833 (alle Teile) bestimmt werden. Beide Ergebnisse werden kombiniert, um die Gesamtfaserzusammensetzung zu bestimmen.
Das Verfahren beruht auf einer Vorabidentifizierung, mittels Lichtmikroskopie, aller Fasern in der Mischung auf der Grundlage ihrer Morphologie nach ISO/TR 11827 [4]. Es ist nicht anzuwenden, wenn Fasern von der gleichen Tierart vorliegen (wie zum Beispiel Mischungen aus Kaschmir und Mohair).
ANMERKUNG 2 In diesem Fall wird die quantitative Analyse mittels mikroskopischer Analyse durchgeführt [zum Beispiel ISO 17751 (alle Teile)].
Textiles - Analyse protéomique qualitative et quantitative de certaines fibres animales - Partie 3: Détection des peptides par LC-MS sans réduction protéique (ISO 20418-3:2020)
Le présent document spécifie une méthode qualitative et quantitative pour déterminer la composition des mélanges de fibres animales ? poils - (composés de laine, de cachemire, de yack, d'alpaga, de chameau ou d'angora) par LC-MS sans réduction protéique.
NOTE 1 La composition des fibres autres que les poils animaux peut être mesurée conformément à l'ISO 1833 (toutes les parties). Les résultats de ces deux méthodes sont ensuite combinés pour déterminer la composition globale des fibres.
La méthode repose sur une identification préliminaire, par microscopie optique, de toutes les fibres présentes dans le mélange sur la base de leur morphologie, conformément à l'ISO/TR 11827[4]. Elle n'est pas applicable si des fibres de la même espèce animale (telles que des mélanges de cachemire et de mohair) sont présentes.
NOTE 2 Dans ce cas, l'analyse quantitative est réalisée par microscopie [tel que décrit dans l'ISO 17751 (toutes les parties), par exemple].
Tekstilije - Kvalitativna in kvantitativna proteomska analiza nekaterih živalskih vlaken - 3. del: Odkrivanje peptida z uporabo LC-MS brez zmanjšanja proteina (ISO 20418-3:2020)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2020
Tekstilije - Kvalitativna in kvantitativna proteomska analiza nekaterih živalskih
vlaken - 3. del: Odkrivanje peptida z uporabo LC-MS brez zmanjšanja proteina (ISO
20418-3:2020)
Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres -
Part 3: Peptide detection using LC-MS without protein reduction (ISO 20418-3:2020)
Textilien - Qualitative und quantitative Proteomanalyse einiger Tierhaarfasern - Teil 3:
Peptiddetektion mit LC-MS ohne Proteinreduktion (ISO 20418-3:2020)
Textiles - Analyse protéomique qualitative et quantitative de certaines fibres animales -
Partie 3: Détection des peptides par LC-MS sans réduction protéique (ISO 20418-
3:2020)
Ta slovenski standard je istoveten z: EN ISO 20418-3:2020
ICS:
59.060.01 Tekstilna vlakna na splošno Textile fibres in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 20418-3
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2020
EUROPÄISCHE NORM
ICS 59.060.01
English Version
Textiles - Qualitative and quantitative proteomic analysis
of some animal hair fibres - Part 3: Peptide detection using
LC-MS without protein reduction (ISO 20418-3:2020)
Textiles - Analyse protéomique qualitative et Textilien - Qualitative und quantitative
quantitative de certaines fibres animales - Partie 3: Proteomanalyse einiger Tierhaarfasern - Teil 3:
Détection des peptides par LC-MS sans réduction Peptiddetektion mit LC-MS ohne Proteinreduktion (ISO
protéique (ISO 20418-3:2020) 20418-3:2020)
This European Standard was approved by CEN on 7 June 2020.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20418-3:2020 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 20418-3:2020) has been prepared by Technical Committee ISO/TC 38
"Textiles" in collaboration with Technical Committee CEN/TC 248 “Textiles and textile products” the
secretariat of which is held by BSI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2021, and conflicting national standards shall
be withdrawn at the latest by January 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 20418-3:2020 has been approved by CEN as EN ISO 20418-3:2020 without any
modification.
INTERNATIONAL ISO
STANDARD 20418-3
First edition
2020-06
Textiles — Qualitative and
quantitative proteomic analysis of
some animal hair fibres —
Part 3:
Peptide detection using LC-MS without
protein reduction
Textiles — Analyse protéomique qualitative et quantitative de
certaines fibres animales —
Partie 3: Détection des peptides par LC-MS sans réduction protéique
Reference number
ISO 20418-3:2020(E)
©
ISO 2020
ISO 20418-3:2020(E)
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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ISO 20418-3:2020(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms . 2
5 Principle . 2
6 Reagents . 3
7 Apparatus . 3
8 Test method . 4
8.1 Sampling . 4
8.2 Preliminary identification . 4
8.3 Wash for degreasing . 4
8.4 Powderization of fibres . 4
8.5 Trypsin digestion . 4
8.6 Marker peptides . 5
8.7 LC-MS analysis . 5
8.8 Evaluation of the validity of observed data . 5
8.9 Calculation of correction factor . 5
8.9.1 Correction factor . 5
8.9.2 Correction factor for Cas1 against She1 and Yak1 (kc). 6
8.9.3 Correction factor for Yak2 against She2, She3 and Cas2 (ky) . 6
8.9.4 Correction factor for alpaca and camel (ka) . 6
8.9.5 Correction factor for angora (kr) . 6
8.10 Calculation of blending ratio . 6
8.10.1 Calculation of blending ratio of cashmere, sheep wool and yak . 6
8.10.2 Calculation by She1, Cas1, Yak1 and kc . 7
8.10.3 Calculation by She2, She3, Cas2, Yak2 and ky . 7
8.10.4 Calculation by She1, She2, She3, Cas2, Yak1 . 7
8.10.5 Calculation of blending ratio of camel, alpaca and angora . 8
9 Test report . 9
Annex A (informative) Marker peptides of cashmere, sheep wool and yak fibres .10
Annex B (informative) Example of LC-MS analysis conditions .13
Annex C (informative) Analysis of camel, alpaca and angora fibres .16
Annex D (informative) Example of marker peptide mass chromatograms .19
Annex E (informative) Results of interlaboratory study .23
Bibliography .24
ISO 20418-3:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
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