EN ISO 15302:2017

SLOVENSKI STANDARD
01-julij-2017
1DGRPHãþD
SIST EN ISO 15302:2010
äLYDOVNHLQUDVWOLQVNHPDãþREHWHUROMD'RORþHYDQMHEHQ]R>D@SLUHQD0HWRGD
WHNRþLQVNHNURPDWRJUDILMHYLVRNHORþOMLYRVWL]UHYHU]QRID]R,62
Animal and vegetable fats and oils - Determination of benzo[a]pyrene - Reverse-phase
high performance liquid chromatography method (ISO 15302:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung des Benzo[a]pyren-Gehalts -
Umkehrphasen-Hochleistungsflüssigchromatographisches Verfahren (ISO 15302:2017)
Corps gras d'origines animale et végétale - Détermination du benzo[a]pyrène - Méthode
par chromatographie liquide à haute performance à polarité de phase inversée (ISO
15302:2017)
Ta slovenski standard je istoveten z: EN ISO 15302:2017
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 15302
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2017
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 15302:2010
English Version
Animal and vegetable fats and oils - Determination of
benzo[a]pyrene - Reverse-phase high performance liquid
chromatography method (ISO 15302:2017)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination du benzo[a]pyrène - Méthode par des Benzo[a]pyren-Gehalts - Umkehrphasen-
chromatographie liquide à haute performance à Hochleistungsflüssigchromatographisches Verfahren
polarité de phase inversée (ISO 15302:2017) (ISO 15302:2017)
This European Standard was approved by CEN on 13 April 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15302:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 15302:2017) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall
be withdrawn at the latest by October 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 15302:2010.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 15302:2017 has been approved by CEN as EN ISO 15302:2017 without any modification.
INTERNATIONAL ISO
STANDARD 15302
Third edition
2017-03
Animal and vegetable fats and oils
— Determination of benzo[a]pyrene
— Reverse-phase high performance
liquid chromatography method
Corps gras d’origines animale et végétale — Détermination du
benzo[a]pyrène — Méthode par chromatographie liquide à haute
performance à polarité de phase inversée
Reference number
ISO 15302:2017(E)
©
ISO 2017
ISO 15302:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
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Tel. +41 22 749 01 11
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copyright@iso.org
www.iso.org
ii © ISO 2017 – All rights reserved

ISO 15302:2017(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 3
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure. 4
10 Expression of results . 6
10.1 Calculation of relative response factors . 6
10.2 Calculation of benzo[a]pyrene content . 7
11 Precision . 7
11.1 Repeatability . 7
11.2 Reproducibility . 7
12 Test report . 7
Annex A (informative) Results of interlaboratory tests. 8
Bibliography . 9
ISO 15302:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO’s adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: w w w . i s o .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11,
Animal and vegetable fats and oils.
This third edition cancels and replaces the second edition (ISO 15302:2007), of which it constitutes a
minor revision. The scope of this document has been revised to exclude its application to milk and milk
products and their derivatives.
iv © ISO 2017 – All rights reserved

INTERNATIONAL STANDARD ISO 15302:2017(E)
Animal and vegetable fats and oils — Determination of
benzo[a]pyrene — Reverse-phase high performance liquid
chromatography method
1 Scope
This document specifies a method for the determination of benzo[a]pyrene in crude or refined edible
oils and fats by reverse-phase high performance liquid chromatography (HPLC) using fluorimetric
detection in the range 0,1 µg/kg to 50 µg/kg.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this
document.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http:// www.e lectropedia. org/
— ISO Online browsing platform: available at http:// www. iso. org/o bp
3.1
benzo[a]pyrene content
mass fraction of benzo[a]pyrene in the test portion, as determined using the method specified in this
document
Note 1 to entry: The content is expressed in micrograms per kilogram.
4 Principle
A test portion is dissolved in light petroleum and benzo[b]chrysene is added as internal standard. A
suitable amount of sample i
...