Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance

SIGNIFICANCE AND USE
This practice provides laboratory procedures for rating the relative bioresistance of metalworking fluid formulations, for determining the need for microbicide addition prior to or during fluid use in metalworking systems and for evaluating microbicide performance. General considerations for microbicide selection are provided in Practice E 2169.
The factors affecting challenge population numbers, taxonomic diversity, physiological state, inoculation frequency and biodeterioration effects in recirculating metalworking fluid systems are varied and only partially understood. Consequently, the results of tests completed in accordance with this practice should be used only to compare the relative performance of products or microbicide treatments included in a test series. Results should not be construed as predicting actual field performance.
SCOPE
1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Methods D 3946 and E 686.
1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change, physical property change or some combination thereof.
1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant components.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Historical
Publication Date
09-Apr-2003
Current Stage
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ASTM E2275-03e1 - Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
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e1
Designation:E2275–03
Standard Practice for
Evaluating Water-Miscible Metalworking Fluid Bioresistance
1
and Antimicrobial Pesticide Performance
This standard is issued under the fixed designation E 2275; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1
e NOTE—Footnote 1 was editorially revised in November 2003.
1. Scope D 3519 Test Method for Foam in Aqueous Media (Blender
Test)
1.1 This practice addresses the evaluation of the relative
D 3601 Test Method for Foam in Aqueous Media (Bottle
inherent bioresistance of water-miscible metalworking fluids,
Test)
the bioresistance attributable to augmentation with antimicro-
D 4012 Test Method for Adenosine Triphosphate (ATP)
bial pesticides or both. It replaces Methods D 3946 and E 686.
Content of Microorganisms in Water
1.2 In this practice relative bioresistance is determined by
D 4627 Method for Iron Chip Corrosion for Water-
challenging metalworking fluids with a biological inoculum
Dilutable Metalworking Fluids
that may either be characterized (comprised of one or more
D 5465 Practice for Determining Microbial Colony Counts
known biological cultures) or uncharacterized (comprised of
from Waters Analyzed by Plating Methods
biologically contaminated metalworking fluid or one or more
E70 Test Method for pH of Aqueous Solutions with the
unidentified isolates from deteriorated metalworking fluid).
Glass Electrode
Challenged fluid bioresistance is defined in terms of resistance
E 1326 Guide for Evaluating Nonconventional Microbio-
to biomass increase, viable cell recovery increase, chemical
logical tests Used for Enumerating Bacteria
property change, physical property change or some combina-
E 2169 Practice for Selecting Antimicrobial Pesticides for
tion thereof.
use in Water-miscible Metalworking Fluids
1.3 This practice is applicable to antimicrobial agents that
2.2 Other Standards:
are incorporated into either the metalworking fluid concentrate
3
4.027 Synthetic Hard Water
or end-use dilution. It is also applicable to metalworking fluids
9215A.6a Heterotrophic Plate Count Media, Plate Count
that are formulated using non-microbicidal, inherently biore-
4
Agar
sistant components.
4
9216 Direct Total Microbial Count
1.4 This standard does not purport to address all of the
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Microbiological Test <71>
safety concerns, if any, associated with its use. It is the
2.3 Government Standard:
responsibility of the user of this standard to establish appro-
40 CFR 156 Labeling Requirements for Pesticides and De-
priate safety and health practices and determine the applica-
vices
bility of regulatory limitations prior to use.
3. Terminology
2. Referenced Documents
2 3.1 Definitions:
2.1 ASTM Standards:
3.1.1 active ingredient, n—thechemicalcomponentorcom-
D 888 Test Methods for Dissolved Oxygen in Water
ponents of an antimicrobial pesticide that provides its micro-
D 1067 Test Methods for Acidity or Alkalinity of Water
bicidal performance.
D 1193 Specification for Reagent Grade Water
3.1.2 antimicrobial pesticide, n—chemical additive regis-
D 3342 Method for Dispersion Stability of New (Unused)
teredunder40 CFR 152,forusetoinhibitgrowth,proliferation
Rolling Oil Dispersions in Water
or both of microorganisms.
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This practice is under the jurisdiction of ASTM Committee E35 on Pesticides
3
and Alternative Control Agents and is the direct responsibility of Subcommittee AOAC International Methods of Analysis, AOAC International, Gaithersburg,
E35.15 on Antimicrobial Agents. MD.
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Current edition approved April 10, 2003. Published May 2003. Available from American Public Health Association (APHA) Standard Meth-
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or ods for the Examination of Water and Wastewater800IStreet,NWWashington,DC
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM 20001.
5
Standards volume information, refer to the standard’s Document Summary page on Available from U.S. Pharmacopeia/National Formulary (USP/NF), 12601
the ASTM website. Twinbrook Parkway Rockville, MD 20852.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
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E2275–03
3.1.3 as supplied, adj—antimicrobial pesticide finished exchanged weekly with fresh fluid before the additional
product including the active ingredient(s), solvent and any challenge. The percentage of fluid exchange should reflect
additional inactive ingredients. anticipated fluid turnover rates in fluid’s end-use application.
3.1.4 biocide, n—any chemical intended f
...

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