ASTM E3162-18
(Practice)Standard Practice Measuring the Durability of Antibacterial Agents Applied to Textiles under Simulated Home Laundering Conditions
Standard Practice Measuring the Durability of Antibacterial Agents Applied to Textiles under Simulated Home Laundering Conditions
SIGNIFICANCE AND USE
5.1 The durability of antimicrobial agents applied to textiles is an important attribute for many of the available technologies on the market. Antimicrobial agents that claim durability are typically fixed ionically, covalently or physically, or both, to a textile surface and are expected to retain their antimicrobial functionality after 5, 25 or 50 washes.
5.2 Textile wash standards do exist that measure features as diverse as colorfastness or softener retention, pilling, or even the appearance of the decorative coatings of a zipper; however, no wash method exists that is specific for measuring the durability of an antimicrobial agent applied directly into or onto a textile surface.
5.3 Current wash standards have been written to either closely simulate (AATCC TM135) or accelerate (AATCC TM61) the laundering conditions that would be experienced during normal home laundering. While shown to be effective when testing physical properties of textiles, these methods introduce variables to the washing protocol that can directly affect the final antimicrobial properties of a fabric. For example, many wash protocols add bleach or softeners which can build up over time and may introduce false positive results in industry standard microbiological tests. Conversely, powdered detergents if not completely rinsed after each wash can leave residual surfactants that can build up over time but are generally removed during wear. These residual detergents can potentially coat an antimicrobial surface and provide false negative results.
5.4 Very specific parameters are identified within this practice to closely replicate home launderings as identified and studied in previous wash protocols (AATCC TM61) and accepted within the textile industry. This practice uses detergents and washing conditions which limit potential cross contamination of samples during washing and unrealistic deposition of residual detergents on the test fabric. These conditions increase the reproducibility...
SCOPE
1.1 To determine the durability of standard antibacterial treatments on textile products such as apparel, piece goods, household articles, hereinafter referred to as “textile” or “textile products” to multiple home launderings.
1.2 This practice subjects textile products treated with antimicrobial agents to multiple simulated and accelerated home launderings under defined parameters such that reproducible and reliable antimicrobial analysis can be performed using standard industry accepted protocols.
1.3 For some antimicrobial agents, the durability of antibacterial properties resulting from exposure to detergent solution and abrasive action of multiple home launderings has been shown to be approximated by one 45-minute laundering cycle. The exact correlation between expressed laundry care instructions and exposure conditions identified in the practice should be determined separately for every antimicrobial agent.
1.4 The subsequent microbiological methods shall be performed by individuals experienced and adept in microbiological procedures and in facilities suitable for the handling of the microorganisms under test.
1.5 This standard may involve hazardous materials, operation, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
General Information
- Status
- Published
- Publication Date
- 31-Mar-2018
- Technical Committee
- E35 - Pesticides, Antimicrobials, and Alternative Control Agents
- Drafting Committee
- E35.15 - Antimicrobial Agents
Relations
- Effective Date
- 01-Nov-2019
- Effective Date
- 01-Apr-2018
- Effective Date
- 01-Mar-2017
- Effective Date
- 15-Sep-2015
- Effective Date
- 01-Sep-2015
- Effective Date
- 01-Apr-2015
- Effective Date
- 15-Jun-2013
- Effective Date
- 15-Jun-2013
- Effective Date
- 15-May-2013
- Effective Date
- 01-Feb-2012
- Effective Date
- 01-Feb-2012
- Effective Date
- 01-May-2010
- Effective Date
- 15-Jan-2009
- Effective Date
- 15-Jan-2009
- Effective Date
- 15-Jan-2009
Overview
ASTM E3162-18 is an internationally recognized standard developed by ASTM International, providing a practice for measuring the durability of antibacterial agents applied to textiles under simulated home laundering conditions. This standard is essential for manufacturers and laboratories aiming to evaluate the effectiveness and lifespan of antimicrobial finishes on textiles such as apparel, household linens, and other fabric goods after repeated wash cycles. ASTM E3162-18 supports reliable and reproducible testing protocols to reflect real-world consumer laundering scenarios, thereby facilitating valid antibacterial durability claims.
Key Topics
Importance of Antibacterial Durability
Assessing antibacterial durability is vital for ensuring textiles retain their antimicrobial function after numerous washes, as advertised. Antimicrobial finishes are typically bonded to textile surfaces and are expected to maintain effectiveness after 5, 25, or even 50 laundering cycles.Simulation of Home Laundering Conditions
Standardized laundering parameters, including temperature, agitation, detergents, and abrasion, are defined to closely replicate home washing conditions. Special care is taken to avoid testing artifacts caused by bleach, softeners, or detergent residues that may affect results.Test Methods and Protocols
The standard outlines the use of steel canisters, standard detergents, defined wash cycles (typically a 45-minute cycle approximates multiple home laundries), and controlled temperatures. Samples are carefully cleaned and conditioned for subsequent microbiological analysis.Reliability and Reproducibility
By minimizing cross-contamination and standardizing wash variables, ASTM E3162-18 increases reproducibility and reliability of antibacterial testing data, allowing valid comparisons across products and technologies.Safety and Competency
The practice addresses the need for appropriate safety, health, and environmental practices, and requires that microbiological testing be performed by qualified personnel in suitable facilities.
Applications
Textile Manufacturing and Quality Control
Textile and apparel manufacturers utilize ASTM E3162-18 to bolster performance claims for antimicrobial textiles, such as athletic wear, bedding, uniforms, and healthcare fabrics. Reliable data supports product labeling and marketing related to sustained antibacterial efficacy.Third-Party Testing and Certification
Accredited laboratories employ this standard to deliver independent verification of antibacterial durability for clients, helping ensure compliance with industry and regulatory expectations.Research and Development
R&D teams apply ASTM E3162-18 to screen new antimicrobial treatments and formulations, ensuring that innovations provide durable protection after real-world laundering.Regulatory and Compliance Processes
The standard provides a recognized methodology for substantiating claims during regulatory submissions or product registration processes, reducing the risk of misleading information in the marketplace.
Related Standards
- ASTM D123 – Terminology Relating to Textiles
- ASTM E2756 – Terminology Relating to Antimicrobial and Antiviral Agents
- ASTM E2922 – Guide for the Use of Standard Test Methods for Evaluating Antibacterial Activity on Textiles
- AATCC TM61 – Colorfastness to Laundering: Accelerated
- AATCC TM135 – Dimensional Changes of Fabrics after Home Laundering
- AATCC Monograph M6 – Standardization of Home Laundry Test Conditions
- ISO 6330 – Textiles – Domestic Washing and Drying Procedures for Textile Testing
Practical Value
Adopting ASTM E3162-18 enhances credibility in antimicrobial textile claims, safeguarding consumer trust and supporting compliance. The practice provides an authoritative process for measuring the durability of antibacterial agents under realistic, reproducible laundering conditions. This is crucial for textiles marketed with hygienic or odor-control features, especially where sustained antimicrobial performance is a key selling point.
Keywords: ASTM E3162-18, antibacterial durability, antimicrobial textiles, home laundering conditions, textile testing standards, ASTM International, antibacterial testing, laundering simulation, textile industry standards.
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Frequently Asked Questions
ASTM E3162-18 is a standard published by ASTM International. Its full title is "Standard Practice Measuring the Durability of Antibacterial Agents Applied to Textiles under Simulated Home Laundering Conditions". This standard covers: SIGNIFICANCE AND USE 5.1 The durability of antimicrobial agents applied to textiles is an important attribute for many of the available technologies on the market. Antimicrobial agents that claim durability are typically fixed ionically, covalently or physically, or both, to a textile surface and are expected to retain their antimicrobial functionality after 5, 25 or 50 washes. 5.2 Textile wash standards do exist that measure features as diverse as colorfastness or softener retention, pilling, or even the appearance of the decorative coatings of a zipper; however, no wash method exists that is specific for measuring the durability of an antimicrobial agent applied directly into or onto a textile surface. 5.3 Current wash standards have been written to either closely simulate (AATCC TM135) or accelerate (AATCC TM61) the laundering conditions that would be experienced during normal home laundering. While shown to be effective when testing physical properties of textiles, these methods introduce variables to the washing protocol that can directly affect the final antimicrobial properties of a fabric. For example, many wash protocols add bleach or softeners which can build up over time and may introduce false positive results in industry standard microbiological tests. Conversely, powdered detergents if not completely rinsed after each wash can leave residual surfactants that can build up over time but are generally removed during wear. These residual detergents can potentially coat an antimicrobial surface and provide false negative results. 5.4 Very specific parameters are identified within this practice to closely replicate home launderings as identified and studied in previous wash protocols (AATCC TM61) and accepted within the textile industry. This practice uses detergents and washing conditions which limit potential cross contamination of samples during washing and unrealistic deposition of residual detergents on the test fabric. These conditions increase the reproducibility... SCOPE 1.1 To determine the durability of standard antibacterial treatments on textile products such as apparel, piece goods, household articles, hereinafter referred to as “textile” or “textile products” to multiple home launderings. 1.2 This practice subjects textile products treated with antimicrobial agents to multiple simulated and accelerated home launderings under defined parameters such that reproducible and reliable antimicrobial analysis can be performed using standard industry accepted protocols. 1.3 For some antimicrobial agents, the durability of antibacterial properties resulting from exposure to detergent solution and abrasive action of multiple home launderings has been shown to be approximated by one 45-minute laundering cycle. The exact correlation between expressed laundry care instructions and exposure conditions identified in the practice should be determined separately for every antimicrobial agent. 1.4 The subsequent microbiological methods shall be performed by individuals experienced and adept in microbiological procedures and in facilities suitable for the handling of the microorganisms under test. 1.5 This standard may involve hazardous materials, operation, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
SIGNIFICANCE AND USE 5.1 The durability of antimicrobial agents applied to textiles is an important attribute for many of the available technologies on the market. Antimicrobial agents that claim durability are typically fixed ionically, covalently or physically, or both, to a textile surface and are expected to retain their antimicrobial functionality after 5, 25 or 50 washes. 5.2 Textile wash standards do exist that measure features as diverse as colorfastness or softener retention, pilling, or even the appearance of the decorative coatings of a zipper; however, no wash method exists that is specific for measuring the durability of an antimicrobial agent applied directly into or onto a textile surface. 5.3 Current wash standards have been written to either closely simulate (AATCC TM135) or accelerate (AATCC TM61) the laundering conditions that would be experienced during normal home laundering. While shown to be effective when testing physical properties of textiles, these methods introduce variables to the washing protocol that can directly affect the final antimicrobial properties of a fabric. For example, many wash protocols add bleach or softeners which can build up over time and may introduce false positive results in industry standard microbiological tests. Conversely, powdered detergents if not completely rinsed after each wash can leave residual surfactants that can build up over time but are generally removed during wear. These residual detergents can potentially coat an antimicrobial surface and provide false negative results. 5.4 Very specific parameters are identified within this practice to closely replicate home launderings as identified and studied in previous wash protocols (AATCC TM61) and accepted within the textile industry. This practice uses detergents and washing conditions which limit potential cross contamination of samples during washing and unrealistic deposition of residual detergents on the test fabric. These conditions increase the reproducibility... SCOPE 1.1 To determine the durability of standard antibacterial treatments on textile products such as apparel, piece goods, household articles, hereinafter referred to as “textile” or “textile products” to multiple home launderings. 1.2 This practice subjects textile products treated with antimicrobial agents to multiple simulated and accelerated home launderings under defined parameters such that reproducible and reliable antimicrobial analysis can be performed using standard industry accepted protocols. 1.3 For some antimicrobial agents, the durability of antibacterial properties resulting from exposure to detergent solution and abrasive action of multiple home launderings has been shown to be approximated by one 45-minute laundering cycle. The exact correlation between expressed laundry care instructions and exposure conditions identified in the practice should be determined separately for every antimicrobial agent. 1.4 The subsequent microbiological methods shall be performed by individuals experienced and adept in microbiological procedures and in facilities suitable for the handling of the microorganisms under test. 1.5 This standard may involve hazardous materials, operation, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
ASTM E3162-18 is classified under the following ICS (International Classification for Standards) categories: 59.080.01 - Textiles in general. The ICS classification helps identify the subject area and facilitates finding related standards.
ASTM E3162-18 has the following relationships with other standards: It is inter standard links to ASTM E2756-19, ASTM E2756-18, ASTM D123-17, ASTM D123-15b, ASTM D123-15a, ASTM D123-15, ASTM D123-13a, ASTM D123-13ae1, ASTM D123-13, ASTM D123-12, ASTM D123-12e1, ASTM E2756-10, ASTM D123-09e2, ASTM D123-09e1, ASTM D123-09. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ASTM E3162-18 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E3162 − 18
Standard Practice
Measuring the Durability of Antibacterial Agents Applied to
Textiles under Simulated Home Laundering Conditions
This standard is issued under the fixed designation E3162; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2.1 ASTM Standards:
1.1 To determine the durability of standard antibacterial
D123Terminology Relating to Textiles
treatments on textile products such as apparel, piece goods,
E2756Terminology Relating toAntimicrobial andAntiviral
household articles, hereinafter referred to as “textile” or
Agents
“textile products” to multiple home launderings.
E2922Guide for The Use of Standard Test Methods and
1.2 This practice subjects textile products treated with
Practices for EvaluatingAntibacterialActivity on Textiles
antimicrobial agents to multiple simulated and accelerated
2.2 AATCC Standards:
home launderings under defined parameters such that repro-
AATCC TM 61: Colorfastness to Laundering: Accelerated
ducible and reliable antimicrobial analysis can be performed
AATCC TM135: Dimensional Changes of Fabrics after
using standard industry accepted protocols.
Home Laundering
1.3 Forsomeantimicrobialagents,thedurabilityofantibac- AATCCMonographM6:StandardizationofHomeLaundry
terial properties resulting from exposure to detergent solution
Test Conditions
and abrasive action of multiple home launderings has been
2.3 ISO Standard:
shown to be approximated by one 45-minute laundering cycle.
ISO 6330Textiles – Domestic washing and drying proce-
The exact correlation between expressed laundry care instruc-
dures for textile testing
tions and exposure conditions identified in the practice should
3. Terminology
be determined separately for every antimicrobial agent.
3.1 Definitions—For definition of terms used in this test
1.4 The subsequent microbiological methods shall be per-
method, refer to: E2756 Standard Terminology Relating to
formed by individuals experienced and adept in microbiologi-
Antimicrobial and Antiviral Agents and D123: Standard Ter-
cal procedures and in facilities suitable for the handling of the
minology Relating to Textiles.
microorganisms under test.
1.5 This standard may involve hazardous materials,
4. Summary of Practice
operation, and equipment. This standard does not purport to
4.1 Fabrics are washed under conditions of temperature,
address all of the safety concerns, if any, associated with its
detergentsolution,andabrasiveactionsimilartothatoccurring
use. It is the responsibility of the user of this standard to
instandardhomelaunderingconditionsusingindustrystandard
establish appropriate safety, health, and environmental prac-
equipment and parameters.
tices and determine the applicability of regulatory limitations
4.2 Singular samples are placed in defined canisters with
prior to use.
detergents and steel balls to represent typical exposures, liquor
1.6 This international standard was developed in accor-
to goods ratios and abrasions experienced in the home laun-
dance with internationally recognized principles on standard-
dering situation. The abrasive action is the result of steel balls
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom-
mendations issued by the World Trade Organization Technical
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Barriers to Trade (TBT) Committee.
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website.
Available from American Association of Textile Chemists and Colorists
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides, (AATCC), P.O. Box 12215, Research Triangle Park, NC 27709-2215, http://
Antimicrobials, and Alternative Control Agents and is the direct responsibility of www.aatcc.org.
Subcommittee E35.15 on Antimicrobial Agents. Available from International Organization for Standardization (ISO), ISO
Current edition approved April 1, 2018. Published July 2018. DOI: 10.1520/ Central Secretariat, BIBC II, Chemin de Blandonnet 8, CP 401, 1214 Vernier,
E3162–18 Geneva, Switzerland, http://www.iso.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E3162 − 18
which,togetherwiththeaggressiveagitationoffixedcanisters, 5.6 This practice is appropriate for porous materials such as
simulatetheabrasiveactionofmultiplefabricsinasingleload. textiles or any porous, soft substrate that is intended to
withstand multiple home washes. This practice is intended to
4.3 After each cycle, samples are removed, rinsed thor-
measure the durable antibacterial properties of such materials.
oughly by hand in water and returned to a fresh solution of
In most instances, further studies will be required to support
detergents and steel balls.
and substantiate actual claims being made for the performance
4.4 After the final cycle, samples are removed, thoroughly
of treated materials in practice or as part of a regulatory
rinsed and dried prior to microbiological testing.
process.
4.5 The use of single canisters during wash allows one to
5.7 This standard practice has been shown to be effective at
measure the durability of an antimicrobial finish without
measuring the durability of polymer based antimicrobial
possible interferences that could occur due to contaminated
agents to home laundering conditions. Particle based or other
ballast, cross contamination of active ingredients from other
antimicrobial agents may require modifications of the current
treated articles or from residual solid detergents. These inter-
methodology to simulate laundering conditions in practice.
ferences are often seen with other standards that are not
The exact correlation between expressed laundry care instruc-
designed specifically for measuring antimicrobial properties.
tions on the antimicrobial treated article and the exposure
conditions identified in the standard practice must be deter-
5. Significance and Use
mined separately for every antimicrobial active.
5.1 The durability of antimicrobial agents applied to
6. Apparatus
textiles is an important attribute for many of the available
technologies on the market. Antimicrobial agents that claim
6.1 accelerated laundering machine—a laundering machine
durabilityaretypicallyfixedionically,covalentlyorphysically, for rotating closed canisters in a thermostatically controlled
or both, to a textile surface and are expected to retain their water bath at 40 6 2 rpm.
antimicrobial functionality after 5, 25 or 50 washes.
6.2 adapter plates, stainless-steel plates for holding canis-
5.2 Textile wash standards do exist that measure features
...




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