SIST EN 1482-4:2024

SLOVENSKI STANDARD
01-junij-2024
Gnojila, sredstva za apnjenje in inhibitorji - Vzorčenje in priprava vzorcev - 4. del:
Vzorčenje za ugotavljanje prisotnosti mikroorganizmov
Fertilizers, liming materials and inhibitors - Sampling and sample preparation - Part 4:
Sampling for microbial presence
Düngemittel, Kalkdünger und Inhibitoren - Probenahme und Probenvorbereitung - Teil 4:
Probenahme für das Vorkommen von Mikroorganismen
Engrais, amendements minéraux basiques et inhibiteurs - Échantillonnage et préparation
de l'échantillon - Partie 4 : Échantillonnage pour déterminer la présence microbienne
Ta slovenski standard je istoveten z: EN 1482-4:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 1482-4
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2024
EUROPÄISCHE NORM
ICS 65.080
English Version
Fertilizers, liming materials and inhibitors - Sampling and
sample preparation - Part 4: Sampling for microbial
presence
Engrais, amendements minéraux basiques et Düngemittel, Kalkdünger und Inhibitoren -
inhibiteurs - Échantillonnage et préparation de Probenahme und Probenvorbereitung - Teil 4:
l'échantillon - Partie 4 : Échantillonnage pour Probenahme für das Vorkommen von
déterminer la présence microbienne Mikroorganismen
This European Standard was approved by CEN on 29 January 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 1482-4:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Sampling . 6
4.1 General requirements . 6
4.2 Sampling types of materials . 6
4.3 Sampling procedure . 7
4.3.1 Maximum quantity of the sampled portion . 7
4.3.2 Number of segments to be sampled . 7
4.3.3 Calculation of the minimum number of sampling points . 8
4.3.4 Calculation of the minimum number of sampling points per segment . 8
4.3.5 Minimum quantity of a segment sample . 8
4.3.6 Collecting the samples . 8
5 Labelling of samples . 9
6 Submission of samples to laboratory . 9
7 Sampling report . 9
8 Multipurpose sampling . 10
Annex A (normative) Multipurpose sampling method . 11
A.1 Sampling strategy . 11
A.2 Sampling purpose . 12
A.3 Sample size required . 13
Bibliography . 14

European foreword
This document (EN 1482-4:2024) has been prepared by Technical Committee CEN/TC 260 “Fertilizers
and liming materials”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2024, and conflicting national standards shall
be withdrawn at the latest by October 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a standardization request given to CEN by the European
Commission and the European Free Trade Association.
EN 1482, Fertilizers, liming materials and inhibitors — Sampling and Sample preparation consists of four
parts:
— Part 1: General sampling provisions;
— Part 2: General sample preparation provisions;
— Part 3: Sampling of static heaps;
— Part 4: Sampling for microbial presence.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
In order to check compliance with the related requirements of Regulation (EU) 2019/1009 [1] methods
have to be specified in standards. In this document the method for obtaining a sample from organic
fertilizers, organo-mineral fertilizers and inorganic fertilizers containing more than 1 % by mass of
organic carbon to detect levels of microbial presence is specified. Any set of segment samples collected
by following the procedures described in this standard is considered to be representative of the sampled
portion.
1 Scope
This document specifies the method for taking a sample from both solid and liquid forms of organic
fertilizers, organo-mineral fertilizers and inorganic fertilizers containing more than 1 % by mass of
organic carbon, when in packages, containers or in bulk, to detect levels of microbial presence. For the
applicability to blends refer to 4.2.4.
This document does not apply to sampling for microbial presence in growing media and soil improvers
(see EN 12579 [6]) or plant biostimulants (see CEN/TS 17702-1 [3]).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 12944-1, Fertilizers and liming materials - Vocabulary - Part 1: General terms
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 12944-1 and the following apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
3.1
batch
total quantity of material present assumed to have the same characteristics
Note 1 to entry: A batch is produced by the same process at the same time, under the same conditions and labelled
in the same manner.
3.2
sampled portion
in relation to sampling for microbiological testing, the quantity of material from the same batch from
which segment samples are taken
3.3
segment
(virtual) part of the sampled portion from which a segment sample is taken for microbiological testing
3.4
segment sample
combination of all incremental samples taken from one segment for microbiological testing to be used as
a laboratory sample
3.5
sampling point
point from which the incremental sample is taken
3.6
incremental sample
quantity of material taken from one sampling point
3.7
solid form
form characterised by structural rigidity and resistance to changes of shape or volume, either in a regular
geometric lattice (crystalline solids) or in an irregular manner (an amorphous solid)
Note 1 to entry: Based on Regulation (EU) 2019/1009 [1], Chapter 1, Article 2, (7).
3.8
liquid form
suspension or solution
Note 1 to entry: Based on Regulation (EU) 2019/1009 [1], Chapter 1, Article 2, (6).
3.9
competent person
person who has acquired through training, qualifications or experience, or a combination of these, the
knowledge and skills enabling that person to perform a specified task
[SOURCE: ISO 17842-1:2015, 3.6 [7]]
4 Sampling
4.1 General requirements
4.1.1 All sampling equipment shall be clean, dry and made from materials which will not contaminate
the sample. Sampling equipment shall be appropriate to the physical characteristics of the product in
order to ensure a representative sample of the product is obtained, i.e. size of individual units of the
product, width and depth of flow of material being sampled in motion. Examples of sampling equipment
for various sampling methods are prescribed in EN 1482-1.
4.1.2 Sampling equipment, including sample containers, shall be subject to a sterilisation process
before use. To avoid cross-contamination, another set of sterilised equipment shall be used to obtain each
individual segment sample. When using new, unopened plastic bags, the bags do not need sterilisation.
4.1.3 Steps shall be taken when sampling, sample handling and sample transport to avoid any sort of
contamination of the sample. Sampling shall be undertaken by a competent person.
4.2 Sampling types of materials
4.2.1 Where a product is chemically declared as a fertilizer but appears physically more like a soil
improver or growing media, and consists totally or mainly of one or more of the following products:
a) organic material, substances or mixtures of these, or
b) plants, plant parts or plant extracts or
c) compost or
d) fresh crop digestates or
e) digestates other than fresh crop digestate.
Sampling shall follow the same sampling plan for microbial sampling for the above type of products as
detailed in EN 12579 [6].
NOTE These categories of products are further defined in Regulation (EU) 2019/1009 [1].
4.2.2 For organic and organo-mineral fertilizers other than those covered by 4.2.1 sampling shall be
carried out as described in 4.3.
4.2.3 Sampling of inorganic fertilizers.
Inorganic macronutrient or micronutrient fertilizer containing more than 1 % organic carbon, with the
exception of organic carbon from certain sources detailed in Regulation (EU) 2019/1009 [1] shall be
sampled as described in 4.3.
NOTE These are the only classes of inorganic fertilizer where maximum micro-organism levels are prescribed
by Regulation (EU) 2019/1009 [1].
4.2.4 Sampling of fertilizing product blends.
When a product blend consists of a mixture of different categories of fertilizing products, and the
sampling method requirements are the same for all the categories, it is possible for the fertilizing product
blend to be sampled for microbial testing using the same requirements.
NOTE If sampling requirements are different then the sampling officer can use the method that seems most
appropriate and, if possible, take samples of the different categories of fertilizing products in the blend.
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