SIST EN 17712:2025

SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17712:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Staphylococcus aureus
Plant biostimulants - Detection of Staphylococcus aureus
Pflanzen-Biostimulanzien - Nachweis von Staphylococcus aureus
Biostimulants des végétaux - Recherche de Staphylococcus aureus
Ta slovenski standard je istoveten z: EN 17712:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 17712
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17712:2022
English Version
Plant biostimulants - Detection of Staphylococcus aureus
Biostimulants des végétaux - Recherche de Pflanzen-Biostimulanzien - Nachweis von
Staphylococcus aureus Staphylococcus aureus
This European Standard was approved by CEN on 26 August 2024.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALIS A T IO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17712:2024 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 6
1 Scope . 7
2 Normative references . 7
3 Terms and definitions . 7
4 Principle . 8
5 Diluents and media . 8
5.1 General . 8
5.2 Modified Giolitti-Cantoni broth. 8
5.3 Baird-Parker RPF agar . 9
6 Apparatus and glassware . 9
7 Sampling . 9
8 Preparation of test sample .10
8.1 General .10
8.2 Solid formulations .10
8.3 Liquid formulations.10
9 Procedure.10
9.1 Enrichment in Giolitti-Cantoni broth .10
9.2 Isolation on Baird-Parker RPF agar .10
9.3 Selection of plates and interpretation .11
10 Expression of results .11
11 Precision .11
12 Test report .11
13 Performance characteristics of the method .12
13.1 Interlaboratory studies .12
13.2 Sensitivity .12
13.3 Specificity .12
13.4 Positive predictive value (PPV) .12
13.5 Negative predictive value (NPV) .12
Annex A (normative) Composition and preparation of culture media and reagents .13
A.1 General .13
A.2 Baird-Parker Rabbit Plasma Fibrinogen (RPF) agar .13
A.3 Modified Giolitti-Cantoni broth.14
Annex B (informative) Repeatability and reproducibility of the method .15
B.1 Materials used in the interlaboratory comparison study .15
B.2 Interlaboratory comparison results .16
B.3 Contingency table analysis .17
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered.18
Bibliography . 19
European foreword
This document (EN 17712:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17712:2022.
— the European foreword, Introduction and Scope have been updated for consistency with other
standards;
— normative references have been updated;
— in Clause 3, the definitions have been revised;
— in Clause 7 and Clause 8, the sampling instructions have been replaced by the relevant normative
reference;
— in Clause 8, the sample preparation instructions have been updated in line with comments received;
— in Clause 9, the procedure has been clarified and references to the media and apparatus required
have been added;
— a new Clause 10 has been added to specify how the results of the test should be expressed;
— in Clause 12, new elements have been added to the test report requirements;
— Annexes A and B have been revised for consistency with other standards;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document specifies a method to verify that the pathogen Staphylococcus aureus is absent from
microbial plant biostimulants. The method is based on the enumeration of coagulase-positive
staphylococci in a sample by counting of colonies obtained on a solid medium (Baird-Parker medium)
after aerobic incubation at 36 °C ± 2 °C.
This document is applicable to all formulations of microbial plant biostimulants in liquid or solid form.
This document is not applicable to other fertilizing products.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Plant Biostimulants and where the following category Plant Biostimulants is the highest
% in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not
the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend
of fertilizing products is composed of components in equal quantity or in case the component EU
fertilising products used for the blend have identical formulations , the user decides which standard to
apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 7218:2024, Microbiology of the food chain — General requirements and guidance for
microbiological examinations (ISO 7218:2024)
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sa
...