SIST EN ISO 23696-1:2026
(Main)Water quality - Determination of nitrate in water using small-scale sealed tubes - Part 1: Dimethylphenol colour reaction (ISO 23696-1:2023)
Water quality - Determination of nitrate in water using small-scale sealed tubes - Part 1: Dimethylphenol colour reaction (ISO 23696-1:2023)
This document specifies a method for the determination of nitrate as NO3-N in water of various origin such as natural water (including groundwater, surface water and bathing water), drinking water and wastewater, in a measuring range of concentration between 0,10 mg/l and 225 mg/l of N03-N using the small-scale sealed tube method. Different measuring ranges of small-scale sealed tube methods can be required.
The measuring ranges can vary depending on the type of the small-scale sealed tube method of different manufacturers.
It is up to the user to choose the small-scale sealed tube test with the appropriate application range or to adapt samples with concentrations exceeding the measuring range of a test by preliminary dilution.
NOTE 1 The results of a sealed-tube test are most precise in the middle of the application range of the test.
Manufacturers' small-scale sealed tube methods are based on dimethylphenol colour reaction depending on the typical operating procedure of the small-scale sealed tube used, see Clause 9.
NOTE 2 Laws, regulations or standards can require that the data is expressed as NO3- after conversion with the stoichiometric conversion factor 4,426 81 in Clause 11.
NOTE 3 In the habitual language, use of sewage treatment and on the displays of automated sealed-tube test devices, NO3 without indication of the negative charge has become the common notation for the parameter nitrate and especially for the parameter nitrate-N. This notation is adopted in this document even though not being quite correct chemical nomenclature.
Wasserbeschaffenheit - Bestimmung von Nitrat in Wasser mittels Küvetten - Teil 1: Verfahren mit Dimethylphenol (ISO 23696-1:2023)
Qualité de l'eau - Détermination du nitrate dans l'eau par la méthode à petite échelle en tubes fermés - Partie 1: Réaction colorimétrique au diméthylphénol (ISO 23696-1:2023)
Le présent document spécifie une méthode de détermination du nitrate en tant que NO3-N dans des eaux d’origines diverses telles que l’eau naturelle (y compris les eaux souterraines, les eaux de surface et les eaux de baignade), l’eau potable et les eaux usées, dans une plage de concentration de mesure comprise entre 0,10 mg/l et 225 mg/l de N03-N en utilisant la méthode à petite échelle en tubes fermés. Différentes plages de mesure des méthodes à petite échelle en tubes fermés peuvent être requises.
Les plages de mesure peuvent varier en fonction du type de méthode à petite échelle en tubes fermés des différents fabricants.
Il appartient à l’utilisateur de choisir l’essai en tubes fermés à petite échelle avec la plage d’application appropriée ou d’adapter les échantillons dont les concentrations dépassent la plage de mesure d’un essai par dilution préliminaire.
NOTE 1 Les résultats d’un essai en tube fermé sont plus précis au milieu de la plage d’application de l’essai.
Les méthodes des fabricants pour les tubes fermés à petite échelle sont basées sur la réaction colorimétrique au diméthylphénol dépendant du mode opératoire type du tube fermé à petite échelle utilisé, voir l’Article 9.
NOTE 2 Les lois, réglementations ou normes peuvent exiger que les données soient exprimées en NO3- après conversion avec le facteur de conversion stœchiométrique 4,426 81 de l’Article 11.
NOTE 3 Dans le langage habituel du traitement des eaux usées et sur les écrans des photomètres ou des spectrophotomètres à essai en tube fermé automatisés, le NO3 sans indication de la charge négative est devenu la notation courante pour le paramètre nitrate et en particulier pour le paramètre nitrate-N. Cette notation est adoptée dans le présent document, même si la nomenclature chimique n’est pas tout à fait correcte.
Kakovost vode - Določanje nitrata v vodi z zatesnjeno kiveto - 1. del: Barvna reakcija z dimetilfenolom (ISO 23696-1:2023)
General Information
- Status
- Published
- Public Enquiry End Date
- 02-Nov-2025
- Publication Date
- 12-Mar-2026
- Technical Committee
- KAV - Water quality
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 28-Jan-2026
- Due Date
- 04-Apr-2026
- Completion Date
- 13-Mar-2026
Relations
- Refers
SIST ISO 5667-10:2021 - Water quality - Sampling - Part 10: Guidance on sampling of waste waters - Effective Date
- 09-Feb-2026
- Effective Date
- 28-Jan-2026
- Effective Date
- 28-Jan-2026
Overview
EN ISO 23696-1:2025 (identical to ISO 23696-1:2023) specifies a photometric method for determination of nitrate as NO3‑N in water using small-scale sealed tubes based on the dimethylphenol colour reaction. The method covers a measuring range of 0.10 mg/L to 225 mg/L NO3‑N (manufacturer ranges may vary). It applies to natural waters (groundwater, surface water, bathing water), drinking water and wastewater and is intended for routine laboratory and field-compatible photometric testing.
Key topics and technical requirements
- Analytical principle: Nitrate reacts in acidic solution with 2,6‑dimethylphenol to form 4‑nitro‑dimethylphenol; absorbance is read by a photometer or spectrophotometer.
- Measuring range: Typical ranges between 0.10 and 225 mg/L NO3‑N; users must select the sealed‑tube kit with an appropriate application range or dilute concentrated samples.
- Reagents and composition: Small-scale tubes contain 2,6‑dimethylphenol, sulfuric acid and phosphoric acid in defined mass‑fraction ranges; typical reading wavelengths: 340 ± 5 nm, 350 ± 5 nm or 365 ± 5 nm.
- Interferences: Common interferents include metals (Ag, Ca, Fe, Cr(VI)), chloride and organic load (COD). Manufacturers must provide interference limits; high chloride may require silver sulfate treatment or dilution.
- Sampling & sample prep: Follow ISO 5667 series guidance. On-site filtration (0.45 μm) is mandatory for wastewater and surface water when required. Sample pH should be 3–10 and reaction temperature 15–25 °C for reproducible colour development.
- Quality control: Calibration verification, system and interference checks, use of certified reference materials (e.g., potassium nitrate), and adherence to instrument calibration stored by manufacturers.
- Result expression: Results may be required as NO3‑ (convert using stoichiometric factor 4.42681) or reported as NO3‑N per regulatory requirements.
- Safety & competence: Tests should be performed by qualified staff following normal laboratory safety practice.
Applications
- Routine monitoring of nitrate in drinking water, groundwater, surface water and wastewater.
- Rapid field or on‑site testing using automated sealed‑tube photometers.
- Regulatory compliance testing, water utility operations, environmental monitoring, and process control in wastewater treatment (nitrification/denitrification process checks).
Who should use this standard
- Environmental and water testing laboratories
- Water utilities and wastewater treatment operators
- Environmental regulators and compliance officers
- Manufacturers of sealed‑tube nitrate test kits and photometric analyzers
- QA/QC managers implementing photometric nitrate testing protocols
Related standards
- ISO 5667‑1, ‑3, ‑10 (sampling guidance and handling)
- ISO 8466‑1 (calibration/evaluation of analytical methods)
- ISO 8655‑2 (pipettes)
- ISO/IEC 17025 (laboratory competence)
Keywords: nitrate testing, nitrate in water, NO3‑N determination, small‑scale sealed tubes, dimethylphenol colour reaction, photometric nitrate analysis, EN ISO 23696‑1:2025, ISO 23696‑1:2023.
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Frequently Asked Questions
SIST EN ISO 23696-1:2026 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Water quality - Determination of nitrate in water using small-scale sealed tubes - Part 1: Dimethylphenol colour reaction (ISO 23696-1:2023)". This standard covers: This document specifies a method for the determination of nitrate as NO3-N in water of various origin such as natural water (including groundwater, surface water and bathing water), drinking water and wastewater, in a measuring range of concentration between 0,10 mg/l and 225 mg/l of N03-N using the small-scale sealed tube method. Different measuring ranges of small-scale sealed tube methods can be required. The measuring ranges can vary depending on the type of the small-scale sealed tube method of different manufacturers. It is up to the user to choose the small-scale sealed tube test with the appropriate application range or to adapt samples with concentrations exceeding the measuring range of a test by preliminary dilution. NOTE 1 The results of a sealed-tube test are most precise in the middle of the application range of the test. Manufacturers' small-scale sealed tube methods are based on dimethylphenol colour reaction depending on the typical operating procedure of the small-scale sealed tube used, see Clause 9. NOTE 2 Laws, regulations or standards can require that the data is expressed as NO3- after conversion with the stoichiometric conversion factor 4,426 81 in Clause 11. NOTE 3 In the habitual language, use of sewage treatment and on the displays of automated sealed-tube test devices, NO3 without indication of the negative charge has become the common notation for the parameter nitrate and especially for the parameter nitrate-N. This notation is adopted in this document even though not being quite correct chemical nomenclature.
This document specifies a method for the determination of nitrate as NO3-N in water of various origin such as natural water (including groundwater, surface water and bathing water), drinking water and wastewater, in a measuring range of concentration between 0,10 mg/l and 225 mg/l of N03-N using the small-scale sealed tube method. Different measuring ranges of small-scale sealed tube methods can be required. The measuring ranges can vary depending on the type of the small-scale sealed tube method of different manufacturers. It is up to the user to choose the small-scale sealed tube test with the appropriate application range or to adapt samples with concentrations exceeding the measuring range of a test by preliminary dilution. NOTE 1 The results of a sealed-tube test are most precise in the middle of the application range of the test. Manufacturers' small-scale sealed tube methods are based on dimethylphenol colour reaction depending on the typical operating procedure of the small-scale sealed tube used, see Clause 9. NOTE 2 Laws, regulations or standards can require that the data is expressed as NO3- after conversion with the stoichiometric conversion factor 4,426 81 in Clause 11. NOTE 3 In the habitual language, use of sewage treatment and on the displays of automated sealed-tube test devices, NO3 without indication of the negative charge has become the common notation for the parameter nitrate and especially for the parameter nitrate-N. This notation is adopted in this document even though not being quite correct chemical nomenclature.
SIST EN ISO 23696-1:2026 is classified under the following ICS (International Classification for Standards) categories: 13.060.50 - Examination of water for chemical substances. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 23696-1:2026 has the following relationships with other standards: It is inter standard links to SIST ISO 5667-10:2021, SIST EN ISO 19111:2020/A2:2024, SIST EN 12255-4:2023. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST EN ISO 23696-1:2026 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2026
Kakovost vode - Določanje nitrata v vodi z zatesnjeno kiveto - 1. del: Barvna
reakcija z dimetilfenolom (ISO 23696-1:2023)
Water quality - Determination of nitrate in water using small-scale sealed tubes - Part 1:
Dimethylphenol colour reaction (ISO 23696-1:2023)
Wasserbeschaffenheit - Bestimmung von Nitrat in Wasser mittels Küvetten - Teil 1:
Verfahren mit Dimethylphenol (ISO 23696-1:2023)
Qualité de l'eau - Détermination du nitrate dans l'eau par la méthode à petite échelle en
tubes fermés - Partie 1: Réaction colorimétrique au diméthylphénol (ISO 23696-1:2023)
Ta slovenski standard je istoveten z: EN ISO 23696-1:2025
ICS:
13.060.50 Preiskava vode na kemične Examination of water for
snovi chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 23696-1
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2025
EUROPÄISCHE NORM
ICS 13.060.50
English Version
Water quality - Determination of nitrate in water using
small-scale sealed tubes - Part 1: Dimethylphenol colour
reaction (ISO 23696-1:2023)
Qualité de l'eau - Détermination du nitrate dans l'eau Wasserbeschaffenheit - Bestimmung von Nitrat in
par la méthode à petite échelle en tubes fermés - Partie Wasser mittels Küvetten - Teil 1: Verfahren mit
1: Réaction colorimétrique au diméthylphénol (ISO Dimethylphenol (ISO 23696-1:2023)
23696-1:2023)
This European Standard was approved by CEN on 14 December 2025.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 23696-1:2025 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
The text of ISO 23696-1:2023 has been prepared by Technical Committee ISO/TC 147 "Water quality”
of the International Organization for Standardization (ISO) and has been taken over as EN ISO 23696-
1:2025 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2026, and conflicting national standards shall be
withdrawn at the latest by June 2026.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 23696-1:2023 has been approved by CEN as EN ISO 23696-1:2025 without any
modification.
INTERNATIONAL ISO
STANDARD 23696-1
First edition
2023-02
Water quality — Determination of
nitrate in water using small-scale
sealed tubes —
Part 1:
Dimethylphenol colour reaction
Qualité de l'eau — Détermination du nitrate dans l'eau par la
méthode à petite échelle en tubes fermés —
Partie 1: Réaction colorimétrique au diméthylphénol
Reference number
ISO 23696-1:2023(E)
ISO 23696-1:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 23696-1:2023(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 2
5 Interferences . 2
6 Sampling and sample preparation . 3
7 Reagents and standard solutions . 3
8 Apparatus . 3
9 Procedure .4
9.1 Reaction conditions for the colour reaction . 4
9.2 Dimethylphenol based colour reaction . 4
10 Quality control . 4
10.1 V erification of the calibration . 4
10.2 System and interferences checks . 5
11 Calculation . 5
12 Expression of results .5
13 Test report . 6
Annex A (informative) Performance data . 7
Bibliography . 8
iii
ISO 23696-1:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2,
Physical, chemical and biochemical methods.
A list of all parts in the ISO 23696 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
ISO 23696-1:2023(E)
Introduction
Nitrate plays an important role in nature and are naturally present in ecosystems. The main anthropic
sources are agriculture (fertilizers and manure) and industrial sources such as food, chemical, paper
companies, etc. and as a result of natural nitrification processes of reduced forms of nitrogen. In turn,
nitrate can be converted to nitrogen gas by denitrifying bacteria. This process takes on strategic
importance within biological water purification plants.
The presence of significant concentrations of nitrate in surface and bathing water, drinking water and
wastewater can pose a risk to human health and/or environment.
v
INTERNATIONAL STANDARD ISO 23696-1:2023(E)
Water quality — Determination of nitrate in water using
small-scale sealed tubes —
Part 1:
Dimethylphenol colour reaction
WARNING — Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document
be carried out by suitably qualified staff.
1 Scope
This document specifies a method for the determination of nitrate as NO -N in water of various origin
such as natural water (including groundwater, surface water and bathing water), drinking water and
wastewater, in a measuring range of concentration between 0,10 mg/l and 225 mg/l of N0 -N using the
small-scale sealed tube method. Different measuring ranges of small-scale sealed tube methods can be
...




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