ISO 18722-2:2026

International
Standard
ISO 18722-2
First edition
Tobacco heating systems —
2026-10
Determination of aerosol collected
mass and nicotine in the aerosol
with a puffing regime according to
the ISO 5501 series —
Part 2:
Aerosol heated tobacco products
(aHTPs)
Systèmes de chauffage du tabac — Détermination de la masse
d'aérosol collecté et de la nicotine dans l'aérosol avec un régime
de fumage conformément à la série ISO 5501 —
Partie 2: Produits de tabac chauffés par un aérosol (PTCa)
Reference number
© ISO 2026
All rights reserved.
This ISO publication is protected by copyright and is owned by ISO and/or its licensors.
The content of this ISO publication is provided under licence, not sold. Use is subject to the applicable licence terms issued by ISO,
an ISO member body, or an authorized third-party distributor.
Except as required for implementation or expressly permitted by a separate licence, no part of this ISO publication may be
reproduced, distributed, modified, used, or made available in any form or by any means – electronic or mechanical, including
photocopying, scanning, recording, or posting on internal or external digital platforms.
Any use beyond the scope of the granted rights is prohibited and may result in legal action.
ISO copyright office
CP 401 • CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 2
7 Preparation . 3
7.1 Preparation of glassware .3
7.2 Preparation of extraction solution .3
7.3 Preparation of calibration solutions .3
8 Procedure . 4
8.1 Preparation of test samples .4
8.2 Glass fibre filter pads handling .4
8.3 Aerosol collection and sample preparation .4
8.4 Test portion .4
8.5 Setting up the apparatus .4
8.6 Calibration of the gas chromatograph .5
8.7 Determination .5
8.7.1 Determination of ACM .5
8.7.2 Determination of THSML .6
8.7.3 Determination of nicotine in the test portion .6
9 Expression of results . 6
10 Repeatability and reproducibility . 6
11 Test report . 7
Annex A (informative) Capacity of glass fibre filter pads . 8
Annex B (informative) Simultaneous analysis of glycerol and propylene glycol with this method . 9
Annex C (informative) Example chromatogram . 10
Bibliography .11

iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
A list of all parts in the ISO 18722 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
Introduction
A tobacco heating system (THS) is a specific combination of a tobacco heating device (THD) and a heated
tobacco product (HTP) used together and designed to produce a nicotine-containing aerosol without
combustion of the tobacco substrate. An HTP is a product containing a tobacco substrate that is designed to
be heated and not combusted by a separate source to produce a nicotine-containing aerosol.
With the emerging development and commercialization of HTPs, there are a number of different approaches
to heating the HTP, categorized as electrically, aerosol and carbon HTPs (eHTP, aHTP and cHTP respectively),
[1]
as described in ISO 6080 . Carbon heated tobacco products (cHTPs) have not been commercially available
as of 2022.
In some countries, the regulation of HTPs requires nicotine yields in aerosol. Therefore, there is a necessity
to have an international standard in place to get reliable/comparable data on nicotine in the aerosol from
THSs, with a specific HTP. Technical investigation of HTPs requires determining aerosol collected mass
(ACM) in combination with a specific THD.
This document was developed for the determination of ACM with a gravimetric method, and nicotine
yields in trapped aHTP aerosol utilizing gas chromatography coupled with a flame ionization detector. This
[2]
document is based on the CORESTA recommended method (CRM) 107 , which was written on the basis of
[3]
the results obtained in an interlaboratory study conducted in 2021 involving 17 laboratories .
No machine puffing regime can represent all human puffing behaviour.
— It is recommended that aHTP also be tested under conditions of a different intensity of machine puffing
than those specified in this document.
— Machine puffing testing is useful to characterize aHTP emissions for design and regulatory purposes, but
communication of machine measurements to users can result in misunderstandings about differences in
exposure and risk across brands.
— Aerosol emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid as measures of human exposure or risks.
Communicating differences between products in machine measurements as differences in exposure or
risk is a misuse of testing using ISO standards.
WARNING — Regulation and standardization are independent from each other, and standardization
does not pre-empt regulation.
v
International Standard ISO 18722-2:2026(en)
Tobacco heating systems — Determination of aerosol
collected mass and nicotine in the aerosol with a puffing
regime according to the ISO 5501 series —
Part 2:
Aerosol heated tobacco products (aHTPs)
1 Scope
[4]
This document specifies an analytical method with a puffing regime according to ISO 5501-2 to
determine aerosol collected mass (ACM) and nicotine in the aerosol from aerosol heated tobacco products,
in combination with a specific tobacco heating device (THD).
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 5501-2, Tobacco heating systems — Definitions and standard conditions for aerosol generation and
collection — Part 2: Aerosol heated tobacco products (aHTPs)
ISO 6080, Tobacco heating systems — Vocabulary
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 6080 and the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
aerosol collected mass
ACM
mass of aerosol collected on an aerosol trapping system from the operation of a tobacco heating system
(THS) by a routine analytical puffing machine
[5]
Note 1 to entry: See ISO 4387 for total particulate matter (TPM).
[6]
[SOURCE: ISO 5501-3:2024 , 3.15]
3.2
tobacco heating system mass loss
THSML
reduction in mass of the tobacco heating system (THS) from its operation by a routine analytical puffing
machine
3.3
combined consumable
combination of one or more consumable types used together over the aerosol collection process
Note 1 to entry: aHTP category may include products having a more frequently changed HTP than the e-liquid based
consumable. Current products have a manufacturer-indicated maximum number of puffs for the aHTP, and termination
of the aerosol collection process should be aligned with this indication.
4 Principle
Aerosol shall be generated and collected as described in ISO 5501-2. The ACM and optionally THSML are
determined gravimetrically. The collected matter is then extracted with propane-2-ol solution containing
internal standard (see 7.2). The nicotine content of an aliquot of the solution is determined by gas
chromatography with flame ionization detection (GC-FID) and quantitated by internal standard calibration.
Results are expressed as the weight of nicotine collected per combined consumable, or per puff as warranted.
5 Reagents
Use only reagents of recognized analytical grade.
1)
5.1 Carrier gas, helium (CAS RN® 7440-59-7), nitrogen (CAS RN® 7727-37-9), or hydrogen (CAS RN®
1333-74-0) of high purity.
5.2 Auxiliary gases, air and hydrogen (CAS RN® 1333-74-0) for flame, and nitrogen (CAS RN® 7727-37-
9) as make-up gas, of high purity, for the flame ionization detector.
5.3 Propane-2-ol (CAS RN® 67-63-0), with maximum water content of 1,0 mg/ml, used with internal
standard(s) to prepare the extraction solution.
NOTE Propane-2-ol is hygroscopic and can absorb water over time if not stored in a sealed container/bottle.
5.4 Internal standards, quinaldine (CAS RN® 91-63-4), n-heptadecane (CAS RN® 629-78-7) or
n-octadecane (CAS RN® 593-45-3) of purity not less than 99 %
...