ISO 21716-1:2020

INTERNATIONAL ISO
STANDARD 21716-1
First edition
Ships and marine technology —
Bioassay methods for screening anti-
fouling paints —
Part 1:
General requirements
PROOF/ÉPREUVE
Reference number
ISO 21716-1:2020(E)
©
ISO 2020

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ISO 21716-1:2020(E)

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Published in Switzerland
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ISO 21716-1:2020(E)

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Preparation of the test panels . 3
4.1 Test procedure — General . 3
4.2 Selection of a material for substrates . 3
4.3 Application of an anti-fouling paint . 4
4.4 Aging of the test panels . 4
4.5 Inspecting and pre-treating of the panels . 4
5 Experimental system . 4
5.1 General . 4
5.2 Water flow-through equipment . 5
Annex A (informative) Aging methods — Examples . 6
Bibliography . 9
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ISO 21716-1:2020(E)

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 8, Ships and marine technology,
Subcommittee SC 2, Marine environment protection, in collaboration with Technical Committee
ISO/TC 35, Paints and varnishes, Subcommittee SC 9, General test methods for paints and varnishes.
A list of all parts in the ISO 21716 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
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ISO 21716-1:2020(E)

Introduction
Anti-fouling paints that contain biocides are widely used to prevent fouling of ship hulls by marine
organisms. Effective anti-fouling technologies are critical to maintaining the fuel consumption
efficiency of ships, minimizing the release of greenhouse gases and other hazardous air pollutants,
and also for minimizing the possible translocation of aquatic species through maritime trade. The
evaluation of anti-fouling paints is generally undertaken by adopting a tiered approach, whereby paint
manufacturers use a battery of laboratory, raft, patch tests and full vessel trials. Raft, patch tests and
full vessel trials are generally conducted over extended periods of time and are predominantly relied
upon for the prediction of coating performance when used commercially on in-service ships.
The results of raft, patch test and full vessel trials (field testing) can be used as part of the regulatory
process for pesticidal or biocidal products in certain countries in order to demonstrate the efficacy
of an anti-fouling paint. Laboratory testing alone is recognized as being unable to predict in-service
performance of efficacy. For example, guidance published by the European Chemical Agency (ECHA) on
the assessment and evaluation of efficacy for anti-fouling products states clearly that laboratory testing
of individual anti-fouling paints is not undertaken as it is not considered to be a realistic evaluation
of the product; field testing, which permits anti-fouling products to be tested under similar operating
conditions and stresses as those encountered when the anti-fouling products are in service is routinely
undertaken instead (see Reference [6]).
Whilst laboratory tests are unable to reliably predict in-service coating performance, they have merit
in the screening of experimental coatings for further evaluation during the research and development
process.
Reproducible objective data obtained by following standardized screening methods, independent of
the test location or the season, can be a useful tool to support the selection of anti-fouling paints for
higher tier testing, e.g. raft or ship tests. ISO 21716 provides a compilation and description of in vitro
bioassay methods intended to aid the process of screening anti-fouling paints prior to higher tier raft
or ship tests. Toxicological screening methods included in each part of ISO 21716 can be used for such
purposes as early decision-making in research and product development, rapid feedback on potential
toxicological concerns, or for the preliminary assessment of anti-fouling paints. For instance, ISO 21716
provides information on methods that can be used to screen anti-fouling paints in order to determine
whether to continue development of an experimental paint and/or a product that contains a particular
ingredient, or to determine whether to take on the cost of performing the remaining tiers within a
complete tiered-testing strategy.
ISO 21716 provides screening bioassays related to certain common genera of fouling organisms, namely
barnacles, mussels and algae. These screening tests are relatively simple and rapid laboratory tests
that can be performed to provide an indication of the toxicity of a painted surface towards selected
test organisms. The screening tests described in each part of ISO 21716 can be used as part of a tiered
approach to predict the ability of an anti-fouling coating to prevent fouling on ships. Alternatively, to
prevent the translocation of invasive marine species by progressively involving subsequent semi-field
(e.g. raft panels) and field testing (e.g. ship trials). On their own, the screening tests described in each
part of ISO 21716 do not reliably predict the ability of an anti-fouling coating to prevent fouling on ships
or the translocation of invasive marine species.
ISO 21716 is not intended to provide a list of validated tests for testing the efficacy of anti-fouling; this
can be covered in regulations. It is not intended to provide a list of validated tests for this purpose,
nor for predicting the ability of a fouling control paint to prevent fouling on ships or to prevent the
translocation of invasive marine species.
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INTERNATIONAL STANDARD ISO 21716-1:2020(E)
Ships and marine technology — Bioassay methods for
screening anti-fouling paints —
Part 1:
General requirements
1 Scope
This document specifies general requirements and common specifications for preparing and aging
panels coated with anti-fouling paint to perform laboratory bioassay screening tests against specified
organisms. Such tests are given in the other parts of the ISO 21716 series, with which this document is
intended to be used.
This document is applicable to all anti-fouling paints that prevent or deter the attachment and growth
of sessile organisms on a surface through chemical or biological means.
It is not applicable to the following:
— coatings that deter or prevent fouling solely by physical means such as biocide-free foul release paints;
— anti-fouling methods used for controlling harmful marine organisms and pathogenic organisms in
ships' ballast water and sediments according to IMO International Convention for the Control and
[7]
Management of Ships' Ballast Water and Sediments, 2004 .
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 2808, Paints and varnishes — Determination of film thickness
ISO 1513, Paints and varnishes — Examination and preparation of test samples
ISO 21716-2, Ships and marine technology — Bioassay methods for screening anti-fouling paints — Part 2:
Barnacles
ISO 21716-3, Ships and marine technology — Bioassay methods for screening anti-fouling paints —
Part 3: Mussels
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
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ISO 21716-1:2020(E)

3.1
aging
providing the test group (3.9) with a surface condition that is more representative of the expected in-
service surface condition of an anti-fouling paint (3.2) than a freshly applied paint surface by exposure
to dynamic and/or static immersion in natural seawater (3.8) or artificial seawater (3.3)
3.2
anti-fouling paint
paint containing biocides (3.4) specifically designed to prevent or deter the attachment and growth of
sessile organisms on a hull surface through chemical or biological means
3.3
artificial seawater
mixture of water and salts to mimic the properties of natural seawater
Note 1 to entry: Water prepared from the mixtures of salts described in ISO 15181-1, ISO 10253, ISO/TS 20787, or
ASTM D 1141-98, and water prepared from commercially available pro
...