ISO 9022-11:2015

INTERNATIONAL ISO
STANDARD 9022-11
Second edition
2015-04-15
Optics and photonics —
Environmental test methods —
Part 11:
Mould growth
Optique et photonique — Méthodes d’essais d’environnement —
Partie 11: Moisissures
Reference number
ISO 9022-11:2015(E)
©
ISO 2015

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ISO 9022-11:2015(E)

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© ISO 2015
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
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Published in Switzerland
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ISO 9022-11:2015(E)

Contents Page
Foreword .iv
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 General information and test conditions . 1
3.1 Test fungi . 1
3.2 Fungal spore suspension . 2
3.2.1 Fungal cultures . 2
3.2.2 Spore suspensions . 2
3.2.3 Mixed spore suspension . 3
3.3 Control strips . 3
3.4 Specimen . 4
3.5 Incubation cabinets and climatic chambers . 5
4 Conditioning method 85: Mould growth . 5
5 Procedure. 5
5.1 General . 5
5.2 Preconditioning . 6
5.3 Activities during conditioning . 6
5.4 Recovery . 6
5.5 Evaluation . 6
5.6 General level for acceptance . 6
6 Environmental test code . 7
7 Specification . 7
Annex A (informative) Explanatory notes . 8
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ISO 9022-11:2015(E)

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 1,
Fundamental standards.
This second edition cancels and replaces the first edition (ISO 9022-11:1994), of which it constitutes a
minor revision.
ISO 9022 consists of the following parts, under the general title Optics and photonics — Environmental
test methods:
— Part 1: Definitions, extent of testing
— Part 2: Cold, heat and humidity
— Part 3: Mechanical stress
— Part 4: Salt mist
— Part 6: Dust
— Part 7: Resistance to drip or rain
— Part 8: High internal pressure, low internal pressure, immersion
— Part 9: Solar radiation and weathering
— Part 11: Mould growth
— Part 12: Contamination
— Part 14: Dew, hoarfrost, ice
— Part 17: Combined contamination, solar radiation
— Part 20: Humid atmosphere containing sulfur dioxide or hydrogen sulfide
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ISO 9022-11:2015(E)

— Part 22: Combined cold, dry heat or temperature change with bump or random vibration
— Part 23: Low pressure combined with cold, ambient temperature and dry and damp heat
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ISO 9022-11:2015(E)

Introduction
Optical instruments are affected during their use by a number of different environmental parameters
which they are required to resist without significant reduction in performance and to remain within
defined specifications.
The type and severity of these parameters depend on the conditions of use of the instrument (for
example, in the laboratory or workshop) and on its geographical location. The environmental effects
on optical instrument performance in the tropics and subtropics are totally different from those found
when they are used in arctic regions. Individual parameters cause a variety of different and overlapping
effects on instrument performance.
The manufacturer attempts to ensure, and the user naturally expects, that instruments will resist the
likely rigours of their environment throughout their life. This expectation can be assessed by exposure
of the instrument to a range of simulated environmental parameters under controlled laboratory
conditions. The severity of these conditions is often increased to obtain meaningful results in a relatively
short period of time.
In order to allow assessment and comparison of the response of optical instruments to appropriate
environmental conditions, ISO 9022 contains details of a number of laboratory tests which reliably
simulate a variety of different environments. The tests are based largely on IEC standards, modified
where necessary to take into account features special to optical instruments.
As a result of continuous progress in all fields, optical instruments are no longer only precision-
engineered optical products, but, depending on their range of application, also contain additional
assemblies from other fields. For this reason, the principal function of the instrument is to be assessed to
determine which International Standard should be used for testing. If the optical function is of primary
importance, then ISO 9022 is applicable, but if other functions take precedence then the appropriate
International Standard in the field concerned should be applied. Cases can arise where application of
both ISO 9022 and other appropriate International Standards will be necessary.
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INTERNATIONAL STANDARD ISO 9022-11:2015(E)
Optics and photonics — Environmental test methods —
Part 11:
Mould growth
WARNING — Although the species of fungi selected for testing do not normally present a hazard
to humans, certain people can develop allergies or other reactions. The use of experienced and
trained personnel is required to ensure the proper handling of fungi and the appropriate conduct
of the tests. It is, therefore, recommended to entrust the performance of the tests required by this
part of ISO 9022 to a microbiological laboratory, since such laboratories have the appropriate
equipment and trained personnel.
1 Scope
This part of ISO 9022 specifies the methods relating to the environmental tests of optical instruments,
including additional assemblies from other fields (e.g. mechanical, chemical, and electronic devices)
under equivalent conditions, for their ability to resist the influence of mould growth.
However, complete instruments or assemblies are only tested as specified in this part of ISO 9022 in
exceptional cases. Normally, representative specimens such as mounted optics, material samples, or
surface coatings on representative samples are used for testing.
The tests described in this part of ISO 9022 are designed for the selection of materials and components
for instruments likely to be used in an environment that is conducive to mould growth, rather than for
regular production control.
The purpose of testing is to investigate to what extent the optical, climatic, mechanical, chemical and electrical
(including electrostatic) performance characteristics of the specimen are affected by mould growth.
In addition, the tests in this part of ISO 9022 are designed to assess to what extent metabolic waste
products (such as enzymes or acids) excreted by fungi, cause etching, corrosion, or short-circuits on, for
instance, printed circuit boards.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 9022-1, Optics and photonics — Environmental test methods — Part 1: Definitions, extent of testing
3 General information and test conditions
3.1 Test fungi
The species of fungi selected for testing (see Table 1) are frequently found on optical glass surfaces.
Among them are species of hydrophyl, mesophyl, and xerophyl fungi.
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ISO 9022-11:2015(E)

Table 1 — Test fungi
Series No. Species
1 Aspergillus niger
2 Aspergillus flavus
3 Aspergillus versicolor
4 Trichoderma viride
5 Penicillium funiculosum
6 Penicillium citrinum
7 Paecilomyces
8 Chaetomium globosum
9 Eurotium tonophilum
10 Aspergillus penicilloiden (Vitrocolae)
Since strains of fungi change their characteristics with time, only fungal species are specified. The test
report or relevant specification, respectively, shall, however, specify the fungal strains used for testing.
3.2 Fungal spore suspension
3.2.1 Fungal cultures
Pure cultures of each of the fungus species specified in Table 1 shall be maintained separately on an
appropriate agar medium (e.g. malt agar).
The fungal cultures used for the spore suspension shall not be older than 14 days to 21 days and shall
not be used more than once for preparing a mixed spore suspension.
3.2.2 Spore suspensions
For preparing the spore suspensions, and wherever else in this Subclause “water” is specified, use
distilled or fully demineralized sterile water containing 0,05 % (mass fraction) of a non-toxic wetting
agent such as sodium dioctylsulfosuccinate or sodium laurylsulfate.
Pour 10 ml of the water into each of the fungal cultures described in 3.2.1.
Using a sterile platinum loop or any other suitable means, carefully scrape the spores from the mycelial
mat. Take care to leave out clumps of agar. Pour the spores charge into a sterile Erlenmeyer flask
containing 45 ml of water. Add sterile solid glass beads and shake vigorously to liberate the spores from
the fruiting body and to break the spore clumps. Filter the dispersed fungal spore suspension through
sterile glass wool to remove mycelial fragments.
Centrifuge the filtrate and discard the supernatant liquid. Resuspend the residue in 50 ml of water and
centrifuge. Wash the spores obtained from each of the fungi in this manner three times.
Dilute the final washed residue with the mineral salts solution specified in Table 2 in such a manner that
each resultant spore suspension contains (1 000 000 ± 200 000) spores per millilitre, measured using a
suitable counting chamber.
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ISO 9022-11:2015(E)

Table 2 — Mineral salts solution
Mass
Component
g
Potassium dihydrogen orthophosphate (KH PO ) 0,7
2 4
Potassium monohydrogen orthophosphate (K HPO ) 0,7
2 4
Magnesium sulfate heptahydrate (MgSO .7H O) 0,7
4 2
Ammonium nitrate (NH NO ) 1,0
4 3
Sodium chloride (NaCl) 0,005
Iron(II) sulfate heptahydrate (FeSO .7H O) 0,002
4 2
Zinc sulfate heptahydrate (ZnSO .7H O) 0,002
4 2
Manganese(II) sulfate monohydrate (MnSO .H O) 0,001
4 2
Distilled water (H O) 1 000,0
2
Sterilize the mineral salts solution in an autoclave at 120 °C for 20 min. Using sodium hydroxide solution,
c(NaOH) = 0,01 mol/l, adjust the pH of the solution to 6,0 to 6,5 after sterilization. (Percentage purity of
the chemicals: atomic adsorption spectroscopy.)
Inoculate each of 10 Petri dishes containing an appropriate agar medium (e.g. malt agar) with spore
suspension and immediately incubate the dishes, to check the viability of each fungus species, in the
incubation chamber to be used for exposing the specimens. In the event that fungicide-treated specimens
are under test in the incubation chamber, expose the Petri dishes to exactly the same climatic conditions
in a separate incubation chamber. The absence of growth of any of the various fungus species, at the end
of one week, will invalidate the results of all simultaneously performed tests using these spores. Such
invalidated tests shall be repeated using freshly prepared m
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