ISO 17293-1:2014

INTERNATIONAL ISO
STANDARD 17293-1
First edition
2014-04-01
Surface active agents — Determination
of chloroacetic acid (chloroacetate) in
surfactants —
Part 1:
HPLC method
Agents de surface — Détermination de l’acide chloroacétique
(chloroacétate) dans les agents tensioactifs —
Partie 1: Méthode CLHP
Reference number
©
ISO 2014
© ISO 2014
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ii © ISO 2014 – All rights reserved

Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Principle . 1
4 Reagents . 1
4.1 General . 1
5 Apparatus . 2
6 Procedures . 2
6.1 HPLC conditions . 2
6.2 Calibration . 3
6.3 Sampling and analysis . 3
7 Results and calculation . 3
7.1 General . 3
7.2 The content of monochloroacetic acid . 4
7.3 The content of dichloroacetic acid . 4
8 Precision . 4
8.1 Repeatability limit . 4
8.2 Reproducibility limit . 4
9 Test report . 4
Annex A (informative) Preliminary treatment of betaine sample . 6
Annex B (informative) Typical chromatogram . 8
Foreword
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The committee responsible for this document is ISO/TC 91, Surface active agents.
ISO 17293 consists of the following parts, under the general title Surface active agents — Determination
of chloroacetic acid (chloroacetate) in surfactants:
— Part 1: HPLC method
— Part 2: Ionic chromatographic method
iv © ISO 2014 – All rights reserved

INTERNATIONAL STANDARD ISO 17293-1:2014(E)
Surface active agents — Determination of chloroacetic acid
(chloroacetate) in surfactants —
Part 1:
HPLC method
1 Scope
This part of ISO 17293 specifies a method for the determination of monochloroacetic acid
(monochloroacetate) and dichloroacetic acid (dichloroacetate) in surfactants by HPLC method.
The method applies for anionic surfactants such as alkyl (phenyl) ethoxylated carboxylate (AEC) or
amphoteric surfactants such as alkyl imidazoline carboxylate, alkyl dimethyl betaine, and fatty acetyl
propyl dimethyl betaine.
The limit of detection (LOD) is ≤0,3 µg/ml for monochloroacetic acid and ≤0,2 µg/ml for dichloroacetic
acid; the limit of quantification (LOQ) is ≤1,0 µg/ml for monochloroacetic acid and ≤0,75 µg/ml for
dichloroacetic acid (using a standard solution).
The LOD, at 5 g of sample weight, is ≤6 mg/kg for monochloroacetic acid and ≤4 mg/kg for dichloroacetic
acid; and the LOQ is ≤20 mg/kg for monochloroacetic acid and ≤15 mg/kg for dichloroacetic acid.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 607, Surface active agents and detergents — Methods of sample division
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results — Part 2: Basic method
for the determination of repeatability and reproducibility of a standard measurement method
3 Principle
The sample is dissolved in the mobile phase in order to analyse by high performance liquid chromatography
(HPLC). After injection, it flows through a C -bonded silicone gel column. The monochloroacetic acid
(monochloroacetate) and dichloroacetic acid (dichloroacetate) are separated in the column
...