EN ISO 16094-2:2025
(Main)Water quality - Analysis of microplastic in water - Part 2: Vibrational spectroscopy methods for waters with low content of suspended solids including drinking water (ISO 16094-2:2025)
Water quality - Analysis of microplastic in water - Part 2: Vibrational spectroscopy methods for waters with low content of suspended solids including drinking water (ISO 16094-2:2025)
This document establishes key principles for the investigation of microplastics in drinking water and water with low content of natural suspended solids using a microscopy technique coupled with vibrational spectroscopy.
This method is applicable to:
— determine the size of microplastics [which range from 1 µm to 5 000 µm], count them and classify them by size range;
— identify the chemical composition of microplastics, the main ones (most used in industry and most abundant in the environment) being: polyethylene (PE), polypropylene (PP), polyethylene terephthalate (PET), polycarbonate (PC), polystyrene (PS), polytetrafluoroethylene (PTFE), polyvinyl chloride (PVC), polyamide (PA), polymethyl methacrylate (PMMA) and polyurethane (PU);
This method is applicable to water with a low content of organic matter and other suspended matter as defined in ISO 6107 (1 mg/l to 100 mg/l or lower when interfering with the determination), i.e.,
— ultrapure water;
— water intended for human consumption;
— raw groundwaters.
Given the very low concentrations of microplastics usually present in these waters, special attention needs to be paid to potential sources of contamination during sample preparation.
This method is intended to determine and characterize large numbers of particles in the sample in automatic mode.
This method can also identify the nature of the other particles that are outside the scope of this document, for example minerals, proteins, cellulose and pigments.
This method does not apply to the characterization of substances intentionally added to or adsorbed on the surface of microplastics. This method does not apply to the determination of the geometric shape of microplastics.
Wasserbeschaffenheit - Analyse von Kunststoff in Wasser - Teil 2: Verfahren mittels Vibrationsspektroskopie (ISO 16094-2:2025)
Dieses Dokument legt wichtige Grundsätze für die Untersuchung von Mikrokunststoffen in Trinkwasser und Wasser mit einem geringen Gehalt an natürlichen Schwebstoffen unter Verwendung eines Mikroskopieverfahrens in Verbindung mit der Schwingungsspektroskopie fest.
Dieses Verfahren ist anwendbar für:
die Ermittlung der Größe von Mikrokunststoffen [die von 1 µm bis 5 000 µm reichen], ihre Zählung sowie die Klassifizierung nach Größenbereichen;
die Bestimmung der chemischen Zusammensetzung von Mikrokunststoffen, wobei die wichtigsten (in der Industrie am meisten verwendeten und in der Umwelt am häufigsten vorkommenden) Mikrokunststoffe Polyethylen (PE), Polypropylen (PP), Polyethylenterephthalat (PET), Polycarbonat (PC), Polystyrol (PS), Polytetrafluorethylen (PTFE), Polyvinylchlorid (PVC), Polyamid (PA), Polymethylmethacrylat (PMMA) und Polyurethan (PU) sind.
Dieses Verfahren ist anwendbar auf Wasser mit einem geringen Gehalt an organischen Stoffen und anderen Schwebstoffen wie in ISO 6107 definiert (1 100 mg/l oder weniger, falls sie die Ermittlung stören), d. h.,
Reinstwasser;
Wasser für den menschlichen Gebrauch;
rohes Grundwasser.
In Anbetracht der sehr geringen Konzentrationen von Mikrokunststoffen, die in diesen Wässern üblicherweise vorhanden sind, ist es erforderlich, bei der Probenvorbereitung besonders auf mögliche Kontaminationsquellen zu achten.
Dieses Verfahren ist für die Ermittlung und Charakterisierung einer großen Anzahl von Partikeln in der Probe im automatischen Modus vorgesehen.
Mit diesem Verfahren kann auch die Beschaffenheit der sonstigen Partikel, die nicht in den Anwendungsbereich dieses Dokuments fallen, z. B. Mineralien, Proteine, Zellulose und Pigmente, bestimmt werden.
Dieses Verfahren gilt nicht für die Charakterisierung von Stoffen, die absichtlich Mikrokunststoffen zugesetzt oder an deren Oberfläche adsorbiert wurden. Dieses Verfahren gilt nicht für die Ermittlung der geometrischen Form von Mikrokunststoffen.
Qualité de l'eau - Analyse des microplastiques dans l'eau - Partie 2: Méthodes de spectroscopie vibrationnelle pour les eaux à faible teneur en matières en suspension, y compris l'eau potable (ISO 16094-2:2025)
Le présent document établit les principes clés de l'étude des microplastiques dans l'eau potable et les eaux à faible teneur en matières en suspension naturelles à l'aide d'une technique de microscopie couplée à une spectroscopie vibrationnelle.
Cette méthode est applicable pour:
— déterminer la taille des microplastiques [qui va de 1 µm à 5 000 µm], les dénombrer et les classer par intervalles de tailles;
— identifier la composition chimique des microplastiques, dont les principaux (les plus utilisés dans l'industrie et les plus présents dans l'environnement) sont les Polyéthylène (PE), Polypropylène (PP), Poly(téréphtalate d'éthylène) (PET), Polycarbonate (PC), Polystyrène (PS), Polytétrafluoroéthylène (PTFE), Polychlorure de vinyle (PVC), Polyamide (PA), Polyméthacrylate de méthyle (PMMA) et Polyuréthane (PU);
Cette méthode est applicable aux eaux à faible teneur en matière organique et en autres matières en suspension comme défini dans l'ISO 6107 (1 mg/l à 100 mg/l ou moins lorsque cela interfère avec la détermination), notamment:
— les eaux ultra pures;
— les eaux destinées à la consommation humaine;
— les eaux souterraines brutes.
Étant donné les très faibles concentrations de microplastiques habituellement présentes dans ces eaux, une attention particulière doit être portée aux possibles sources de contamination lors de la préparation de l'échantillon.
Cette méthode a pour but de déterminer et de caractériser un grand nombre de particules dans l'échantillon en mode automatique.
Cette méthode peut aussi identifier la nature des autres particules qui n'entrent pas dans le domaine d'application du présent document, par exemple les minéraux, les protéines, la cellulose et les pigments.
Cette méthode ne s'applique pas à la caractérisation des substances intentionnellement ajoutées ou adsorbées à la surface des microplastiques. Cette méthode ne s'applique pas à la détermination de la forme géométrique de microplastiques.
Kakovost vode - Analiza mikroplastike v vodi - 2. del: Metode vibracijske spektroskopije za vodo z nizko vsebnostjo suspendiranih delcev, vključno s pitno vodo (ISO 16094-2:2025)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN ISO 16094-2:2024
01-januar-2024
Kakovost vode - Analiza mikroplastike v vodi - 2. del: Metode vibracijske
spektroskopije za vodo z nizko vsebnostjo suspendiranih trdnih delcev, vključno s
pitno vodo (ISO/DIS 16094-2:2023)
Water quality - Analysis of microplastic in water - Part 2: Vibrational spectroscopy
methods for waters with low content of suspended solids including drinking water
(ISO/DIS 16094-2:2023)
Wasserbeschaffenheit - Analyse von Kunststoff in Wasser - Teil 2: Verfahren mittels
Vibrationsspektroskopie (ISO/DIS 16094-2:2023)
Qualité de l'eau - Analyse des microplastiques dans l'eau - Partie 2: Méthodes de
spectroscopie vibrationnelle pour les eaux à faible teneur en matières en suspension, y
compris l'eau potable (ISO/DIS 16094-2:2023)
Ta slovenski standard je istoveten z: prEN ISO 16094-2
ICS:
13.060.45 Preiskava vode na splošno Examination of water in
general
oSIST prEN ISO 16094-2:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
oSIST prEN ISO 16094-2:2024
oSIST prEN ISO 16094-2:2024
DRAFT INTERNATIONAL STANDARD
ISO/DIS 16094-2
ISO/TC 147/SC 2 Secretariat: DIN
Voting begins on: Voting terminates on:
2023-11-21 2024-02-13
Water quality — Analysis of microplastic in water —
Part 2:
Vibrational spectroscopy methods for waters with low
content of suspended solids including drinking water
ICS: 13.060.45
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
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NATIONAL REGULATIONS.
ISO/DIS 16094-2:2023(E)
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PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023
oSIST prEN ISO 16094-2:2024
ISO/DIS 16094-2:2023(E)
DRAFT INTERNATIONAL STANDARD
ISO/DIS 16094-2
ISO/TC 147/SC 2 Secretariat: DIN
Voting begins on: Voting terminates on:
Water quality — Analysis of microplastic in water —
Part 2:
Vibrational spectroscopy methods for waters with low
content of suspended solids including drinking water
ICS: 13.060.45
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
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NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 16094-2:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
Published in Switzerland
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023
oSIST prEN ISO 16094-2:2024
ISO/DIS 16094-2:2023(E)
Contents Page
Foreword .v
Introduction . vi
1 Scope . 1
2 Normative references . 2
3 Terms, definitions and abbreviations . 2
3.1 Terms and definitions . 2
3.2 Abbreviations . 3
4 Principle . 4
5 Interferences . 4
6 Reagents, consumables and reference materials . 4
6.1 Particle-free water of known quality . 4
6.2 Detergent solution . 5
6.3 Ethanol . 5
6.4 Plastic reference material . . 5
6.5 Containers for filter transportation . 5
7 Precautions for the laboratory environment, apparatus and materials .5
7.1 Operating precautions linked to the laboratory environment . 5
7.2 Equipment . 5
7.2.1 Infrared (IR) apparatus coupled to microscope, consisting of: . 5
7.2.2 Raman coupled to microscope, consisting of: . 6
7.2.3 Filtration system . 6
7.2.4 Characteristics of filters: type and sizes. 7
7.3 Precautions and cleaning protocol for materials . 7
7.4 Bottles or containers for sampling . 8
8 Sampling . 8
9 Operating protocol . 8
9.1 Volume of test sample . 8
9.2 Samples filtration protocol . 8
9.3 Analytical control blanks . 9
9.4 Adjustment and calibration of instruments . 9
9.4.1 Infrared microscope . 9
9.4.2 Raman microscope . 10
9.5 Cartesian coordinates of analysed particles. 10
9.6 Analysis of the filter surface . 10
9.6.1 Choice of the optical objectives . 10
9.6.2 Selection of particles to be analysed or choice of the analysed surface area. 10
9.6.3 TOTAL Model . 11
9.6.4 RANDOM Model. 11
9.6.5 CAKE Model .12
9.6.6 SPIRAL or HELIX Model . 13
9.6.7 Particles enumeration and size description .13
9.6.8 IR spectra acquisition and particles identification .13
9.6.9 Raman spectra acquisition and particles identification . 14
9.7 Sampling and analytical control blank analysis . 14
9.8 Spectra treatment . 14
9.9 Criteria of chemical composition identification. 15
9.10 Spectral interferences .15
10 Method characterization and verification .16
10.1 Verification of the particles size measurement accuracy . 16
10.2 Verification of microplastics identification and classification at claimed size . 16
iii
oSIST prEN ISO 16094-2:2024
ISO/DIS 16094-2:2023(E)
10.3 Determination of minimal HQI for automatic identification . 16
10.4 Determination of the reporting limits of the method . 17
10.5 Verification of microplastics recovery rate of the method . 17
11 Quality check of analytical control blanks in test series .18
12 Expression of results .18
13 Test report .19
Annex A (informative) List of characteristic wave numbers for the main polymers .20
Annex B (informative) Creation of an internal spectral database .23
Annex C (informative) Description of known interference during acquisition of spectral
fingerprint — Presence of pigments .
...
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