EN ISO 11816-1:2024
(Main)Milk and milk products - Determination of alkaline phosphatase activity - Part 1: Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 1: Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)
This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks.
This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l.
Milch und Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase - Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816‑1:2024)
Dieses Dokument legt ein fluorimetrisches Verfahren für die Bestimmung der Aktivität der alkalischen Phosphatase (ALP, EC 3.1.3.1) in Rohmilch, wärmebehandelter Vollmilch, fettarmer Milch, Magermilch und aromatisierter Milch fest.
Dieses Verfahren ist anwendbar für Milch und flüssige Milchprodukte von Kühen, Schafen und Ziegen. Es ist auch anwendbar für Milchpulver nach Rekonstitution.
Das Gerät zur Bestimmung von ALP kann Aktivitäten von bis zu 7 000 Milliunits je Liter (mU/l) auslesen. Wenn die Aktivität größer als 7 000 mU/l ist, dann wird mit ALP-freier Milch verdünnt, um einen Messwert von nicht mehr als 7 000 mU/l zu erhalten.
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1: Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2024)
Le présent document spécifie une méthode fluorimétrique pour la détermination de l’activité de la phosphatase alcaline (ALP) (EC 3.1.3.1) dans les laits crus et traités thermiquement entiers, demi‑écrémés, écrémés et les laits aromatisés.
La méthode est applicable au lait et aux boissons à base de lait de vache, de brebis et de chèvre. Elle est également applicable au lait en poudre après reconstitution.
L’instrument utilisé pour la détermination de l’ALP peut lire des activités jusqu’à 7 000 mU/l (milliunités par litre). Si l’activité est supérieure à 7 000 mU/l, une dilution avec du lait exempt d’ALP est effectuée de façon à obtenir un mesurage ne dépassant pas 7 000 mU/l.
Mleko in mlečni izdelki- Določanje aktivnosti alkalne fosfataze - 1. del: Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2024)
Ta dokument določa fluorimetrijsko metodo za določanje aktivnosti alkalne fosfataze (ALP) (EC
3.1.3.1) v surovem in toplotno obdelanem polnomastnem, polmastnem in posnetem mleku in mleku z okusom.
Ta metoda se uporablja za mleko in pijače na osnovi kravjega, ovčjega in kozjega mleka. Uporablja se tudi za mleko v prahu po pripravi.
Instrument, ki se uporablja za določanje alkalne fosfataze, lahko beleži aktivnosti do 7000 milienot na liter (mU/l).
Če je aktivnost višja od 7000 mU/l, se razredči z mlekom brez vsebnosti alkalne fosfataze, da se pridobi meritev, ki ne presega 7000 mU/l.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2024
Nadomešča:
SIST EN ISO 11816-1:2014
Mleko in mlečni izdelki- Določanje aktivnosti alkalne fosfataze - 1. del:
Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 1:
Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)
Milch und Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase -
Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816-
1:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1:
Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-1:2024
ICS:
67.100.10 Mleko in predelani mlečni Milk and processed milk
proizvodi products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 11816-1
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2024
EUROPÄISCHE NORM
ICS 67.100.10 Supersedes EN ISO 11816-1:2013
English Version
Milk and milk products - Determination of alkaline
phosphatase activity - Part 1: Fluorimetric method for milk
and milk-based drinks (ISO 11816-1:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milcherzeugnisse - Bestimmung der
la phosphatase alcaline - Partie 1: Méthode Aktivität der alkalischen Phosphatase - Teil 1:
fluorimétrique pour le lait et les boissons à base de lait Fluorimetrisches Verfahren für Milch und flüssige
(ISO 11816-1:2024) Milchprodukte (ISO 11816-1:2024)
This European Standard was approved by CEN on 25 March 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-1:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 11816-1:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-1:2013.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 11816-1:2024 has been approved by CEN as EN ISO 11816-1:2024 without any
modification.
International
Standard
ISO 11816-1
IDF 155-1
Fourth edition
Milk and milk products —
2024-01
Determination of alkaline
phosphatase activity —
Part 1:
Fluorimetric method for milk and
milk-based drinks
Lait et produits laitiers — Détermination de l'activité de la
phosphatase alcaline —
Partie 1: Méthode fluorimétrique pour le lait et les boissons à
base de lait
Reference numbers
ISO 11816-1:2024(en)
IDF 155-1:2024(en) © ISO and IDF 2024
ISO 11816-1:2024(en)
IDF 155-1:2024(en)
© ISO and IDF 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: +41 22 749 01 11 Phone: +32 2 325 67 40
Fax: +32 2 325 67 41
Email: copyright@iso.org Email: info@fil-idf.org
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
ISO 11816-1:2024(en)
IDF 155-1:2024(en)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparations . 4
8.1 Alkaline phosphatase-free milk .4
8.2 Preparation of the test sample .4
8.2.1 General .4
8.2.2 Pasteurized test samples .4
8.2.3 Dilution of test samples with high ALP values .4
9 Procedure . 4
9.1 Verification of instrument performance .4
9.1.1 General .4
9.1.2 Daily instrument tests .5
9.1.3 Using FLM200 .5
9.1.4 Using FLM300 .5
9.1.5 Controls .5
9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3) .6
9.3 Calibration .6
9.3.1 General .6
9.3.2 Using FLM200 .6
9.3.3 Using FLM300 .6
9.4 Determination .7
9.5 Test-sample-related controls .8
9.5.1 Recommended negative and positive control tests .8
9.5.2 Interfering substance test .8
9.5.3 Heat-stable microbial alkaline phosphatase control test .8
10 Calculation and expression of results . 8
10.1 Calibration ratio .8
10.2 Calculation .9
10.3 Expression of test results.9
11 Precision . 9
11.1 Interlaboratory study .9
11.2 Repeatability .
...
SLOVENSKI STANDARD
01-marec-2024
Nadomešča:
SIST EN ISO 11816-1:2014
Mleko in mlečni proizvodi - Določanje aktivnosti alkalne fosfataze - 1. del:
Fluorimetrijska metoda za mleko in pijače na osnovi mleka (ISO 11816-1:2024)
Milk and milk products - Determination of alkaline phosphatase activity - Part 1:
Fluorimetric method for milk and milk-based drinks (ISO 11816-1:2024)
Milch und Milcherzeugnisse - Bestimmung der Aktivität der alkalischen Phosphatase -
Teil 1: Fluorimetrisches Verfahren für Milch und flüssige Milchprodukte (ISO 11816-
1:2024)
Lait et produits laitiers - Détermination de l'activité de la phosphatase alcaline - Partie 1:
Méthode fluorimétrique pour le lait et les boissons à base de lait (ISO 11816-1:2024)
Ta slovenski standard je istoveten z: EN ISO 11816-1:2024
ICS:
67.100.10 Mleko in predelani mlečni Milk and processed milk
proizvodi products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 11816-1
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2024
EUROPÄISCHE NORM
ICS 67.100.10 Supersedes EN ISO 11816-1:2013
English Version
Milk and milk products - Determination of alkaline
phosphatase activity - Part 1: Fluorimetric method for milk
and milk-based drinks (ISO 11816-1:2024)
Lait et produits laitiers - Détermination de l'activité de Milch und Milcherzeugnisse - Bestimmung der
la phosphatase alcaline - Partie 1: Méthode Aktivität der alkalischen Phosphatase - Teil 1:
fluorimétrique pour le lait et les boissons à base de lait Fluorimetrisches Verfahren für Milch und flüssige
(ISO 11816-1:2024) Milchprodukte (ISO 11816-1:2024)
This European Standard was approved by CEN on 25 March 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11816-1:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 11816-1:2024) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by July 2024, and conflicting national standards shall be
withdrawn at the latest by July 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 11816-1:2013.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 11816-1:2024 has been approved by CEN as EN ISO 11816-1:2024 without any
modification.
International
Standard
ISO 11816-1
IDF 155-1
Fourth edition
Milk and milk products —
2024-01
Determination of alkaline
phosphatase activity —
Part 1:
Fluorimetric method for milk and
milk-based drinks
Lait et produits laitiers — Détermination de l'activité de la
phosphatase alcaline —
Partie 1: Méthode fluorimétrique pour le lait et les boissons à
base de lait
Reference numbers
ISO 11816-1:2024(en)
IDF 155-1:2024(en) © ISO and IDF 2024
ISO 11816-1:2024(en)
IDF 155-1:2024(en)
© ISO and IDF 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
CP 401 • Ch. de Blandonnet 8 Silver Building • Bd Auguste Reyers 70/B
CH-1214 Vernier, Geneva B-1030 Brussels
Phone: +41 22 749 01 11 Phone: +32 2 325 67 40
Fax: +32 2 325 67 41
Email: copyright@iso.org Email: info@fil-idf.org
Website: www.iso.org Website: www.fil-idf.org
Published in Switzerland
ii
ISO 11816-1:2024(en)
IDF 155-1:2024(en)
Contents Page
Forewords .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sampling . 4
8 Preparations . 4
8.1 Alkaline phosphatase-free milk .4
8.2 Preparation of the test sample .4
8.2.1 General .4
8.2.2 Pasteurized test samples .4
8.2.3 Dilution of test samples with high ALP values .4
9 Procedure . 4
9.1 Verification of instrument performance .4
9.1.1 General .4
9.1.2 Daily instrument tests .5
9.1.3 Using FLM200 .5
9.1.4 Using FLM300 .5
9.1.5 Controls .5
9.2 Reagent controls to test the suitability of ready-to-use working substrate (5.3) .6
9.3 Calibration .6
9.3.1 General .6
9.3.2 Using FLM200 .6
9.3.3 Using FLM300 .6
9.4 Determination .7
9.5 Test-sample-related controls .8
9.5.1 Recommended negative and positive control tests .8
9.5.2 Interfering substance test .8
9.5.3 Heat-stable microbial alkaline phosphatase control test .8
10 Calculation and expression of results . 8
10.1 Calibration ratio .8
10.2 Calculation .9
10.3 Expression of test results.9
11 Precision . 9
11.1 Interlaboratory study .9
11.2 Repeatability .
...
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