EN ISO 17059:2019
(Main)Oilseeds - Extraction of oil and preparation of methyl esters of triglyceride fatty acids for analysis by gas chromatography (Rapid method) (ISO 17059:2019)
Oilseeds - Extraction of oil and preparation of methyl esters of triglyceride fatty acids for analysis by gas chromatography (Rapid method) (ISO 17059:2019)
This document specifies a rapid method for extraction of oil and for preparation of the methyl esters of fatty acids. The methyl esters thus obtained can be used for gas chromatography.
This document is applicable to the following oilseeds: rape and mustard with low erucic acid content (< 2 %), sunflower, soya beans, linseed.
NOTE Applying this rapid method to high erucic acid content rapeseed leads to an overestimation of erucic acid content by approximately a mass fraction of 1 %. This difference was observed in Reference [6] and could be due to the partial extraction of the oil from the sample (yield around 70 %). High content of erucic acid in triglycerides could increase their solubility in hexane because of the lipophilic effect of the carbon long-chain (C22). However, as this effect was not checked on a large set of high erucic rapeseed samples, it is not appropriate to apply a correction factor to the erucic acid content when analysing high erucic acid rapeseed.
Ölsaaten - Extraktion von Öl und Herstellung von Methylester aus den Fettsäuren der Triglyceride für die Analyse durch Gaschromatographie (Schnellverfahren) (ISO 17059:2019)
Dieses Dokument legt ein Schnellverfahren für die Extraktion von Öl und die Herstellung der Methylester der Fettsäuren fest. Die dabei gewonnenen Methylester können für die Gaschromatographie verwendet werden.
Dieses Dokument ist für die folgenden Ölsaaten anwendbar: Raps und Senf mit niedrigem Erucasäuregehalt (< 2 %), Sonnenblume, Sojabohnen, Leinsamen.
ANMERKUNG Die Anwendung dieses Schnellverfahrens bei Rapssamen mit hohem Erucasäuregehalt führt zu einer Überbewertung des Erucasäuregehaltes bis zu ungefähr einem Massenanteil von 1 %. Dieser Unterschied wurde in einem Ringversuch, siehe Literaturhinweis [6], beobachtet, und könnte sich aufgrund der teilweisen Extraktion des Öls aus der Probe (Ausbeute etwa 70 %) ergeben. Der hohe Erucasäuregehalt in Triglyceriden könnte aufgrund der lipophilen Wirkung des langkettigen Kohlenstoffs (C22) deren Löslichkeit in Hexan erhöhen. Da diese Wirkung jedoch nicht an einer großen Menge von Rapssamenproben mit hohem Erucasäuregehalt überprüft wurde, ist es bei der Analyse von Rapssamen mit hohem Erucasäuregehalt nicht angebracht, einen Korrekturfaktur auf den Erucasäuregehalt anzuwenden.
Graines oléagineuses - Extraction de l'huile et préparation des esters méthyliques d'acides gras de triglycérides pour analyse par chromatographie en phase gazeuse (Méthode rapide) (ISO 17059:2019)
Le présent document spécifie une méthode rapide pour l'extraction de l'huile et la préparation des esters méthyliques d'acides gras. Les esters méthyliques ainsi obtenus peuvent être utilisés pour la chromatographie en phase gazeuse.
Le présent document s'applique aux graines oléagineuses suivantes: colza et moutarde à faible teneur en acide érucique (< 2 %), tournesol, soja, lin.
NOTE L'application de la présente méthode rapide au colza à forte teneur en acide érucique conduit à une teneur surestimée en acide érucique de l'ordre de 1 % (fraction massique). Cette différence a été observée dans la Référence [6] et pourrait être liée à l'extraction partielle de l'huile de l'échantillon (rendement de l'ordre de 70 %). Une forte teneur en acide érucique dans les triglycérides pourrait accroître leur solubilité dans l'hexane en raison de l'effet lipophile de la longue chaîne de carbone (C22). Toutefois, cet effet n'ayant pas été vérifié sur un grand ensemble d'échantillons de colza à forte teneur en acide érucique, il n'est pas approprié d'appliquer de facteur de correction à cette teneur pour l'analyse du colza à forte teneur en acide érucique.
Oljnice - Ekstrakcija olja in priprava metilnih estrov iz trigliceridnih maščobnih kislin za analizo s plinsko kromatografijo (hitra metoda) (ISO 17059:2019)
Ta dokument določa hitro metodo za ekstrakcijo olja in pripravo metilnih estrov iz maščobnih kislin. Tako pridobljene metilne estre je mogoče uporabiti za plinsko kromatografijo. Ta mednarodni standard se uporablja za naslednje oljnice: oljno repico, sončnice, sojo, gorčično seme, laneno seme. OPOMBA: Uporaba te hitre metode za visoko vsebnost eruka kisline oljne repice vodi do precenjevanja vsebnosti eruka kisline.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2019
Nadomešča:
SIST EN ISO 17059:2009
Oljnice - Ekstrakcija olja in priprava metilnih estrov iz trigliceridnih maščobnih
kislin za analizo s plinsko kromatografijo (hitra metoda) (ISO 17059:2019)
Oilseeds - Extraction of oil and preparation of methyl esters of triglyceride fatty acids for
analysis by gas chromatography (Rapid method) (ISO 17059:2019)
Ölsamen - Extraktion von Öl und Herstellung von Methylester aus den Fettsäuren der
Triglyceride für die Analyse durch Gaschromatographie (Schnellverfahren) (ISO
17059:2019)
Graines oléagineuses - Extraction de l'huile et préparation des esters méthyliques
d'acides gras de triglycérides pour analyse par chromatographie en phase gazeuse
(Méthode rapide) (ISO 17059:2019)
Ta slovenski standard je istoveten z: EN ISO 17059:2019
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 17059
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2019
EUROPÄISCHE NORM
ICS 67.200.20 Supersedes EN ISO 17059:2009
English Version
Oilseeds - Extraction of oil and preparation of methyl
esters of triglyceride fatty acids for analysis by gas
chromatography (Rapid method) (ISO 17059:2019)
Graines oléagineuses - Extraction de l'huile et Ölsaaten - Extraktion von Öl und Herstellung von
préparation des esters méthyliques d'acides gras de Methylester aus den Fettsäuren der Triglyceride für
triglycérides pour analyse par chromatographie en die Analyse durch Gaschromatographie
phase gazeuse (Méthode rapide) (ISO 17059:2019) (Schnellverfahren) (ISO 17059:2019)
This European Standard was approved by CEN on 28 July 2019.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
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EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17059:2019 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 17059:2019) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2020, and conflicting national standards shall
be withdrawn at the latest by March 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 17059:2009.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 17059:2019 has been approved by CEN as EN ISO 17059:2019 without any modification.
INTERNATIONAL ISO
STANDARD 17059
Second edition
2019-07
Oilseeds — Extraction of oil and
preparation of methyl esters of
triglyceride fatty acids for analysis by
gas chromatography (rapid method)
Graines oléagineuses — Extraction de l'huile et préparation des
esters méthyliques d'acides gras de triglycérides pour analyse par
chromatographie en phase gazeuse (méthode rapide)
Reference number
ISO 17059:2019(E)
©
ISO 2019
ISO 17059:2019(E)
© ISO 2019
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Published in Switzerland
ii © ISO 2019 – All rights reserved
ISO 17059:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Sampling . 2
8 Procedure. 2
8.1 Preparation of the test sample . 2
8.2 Test portion . 3
8.2.1 General. 3
8.2.2 Case of grains having an oil content exceeding 30 % by mass (rape,
mustard, sunflower and linseed) . 3
8.2.3 Case of grains having an oil content between 15 % and 30 % by mass
(soya beans) . 3
8.3 Extraction of the oil . 3
8.4 Filtration and drying of the extract . 3
8.5 Preparation of the methyl esters . 3
9 Test report . 4
Annex A (normative) General analytical procedures . 5
Bibliography . 6
ISO 17059:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
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For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 2,
Oleaginous seeds and fruits and oilseed meals.
This second edition cancels and replaces the first edition (ISO 17059:2007), which has been technically
revised. The main changes compared with the previous edition are as follows:
— the description of the preparation of methyl esters in 8.5 has been updated to remove the reference
to ISO 5509:2000, which has been withdrawn.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved
ISO 17059:2019(E)
Introduction
Chromatographic analysis of the fatty acid methyl esters (FAME) of oilseeds requires oil extraction
from the oilseeds. The methods usually performed in laboratories involve oil extraction for the
[2][3]
determination of oil content and are tedious or time consuming . Consequently, the total duration
and cost of the analysis of triglyceride fatty acids in oilseeds, including oil extraction, preparation and
gas chromatography of the FAME are considerably increased by the oil extraction step.
This document specifies a rapid and optimized method for a combined oil extraction and FAME
preparation. The oil is only partially extracted from the seeds and the extracted fraction remains
representative enough of the total content when the method is applied to the seeds specified in
[4][5]
the Scope . The FAME are prepared according to the transesterification method described in
...
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