CEN/TS 16868:2015

SLOVENSKI STANDARD
01-februar-2016
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Ambient air - Sampling and analysis of airborne pollen grains and fungal spores for
allergy networks - Volumetric Hirst method
Außenluft - Probenahme und Analyse luftgetragener Pollen und Pilzsporen für
Allergienetzwerke - Volumetrische Hirst-Methode
Air ambiant - Échantillonnage et analyse des grains de pollen et des spores fongiques
aériens pour les réseaux aérobiologiques - Méthode volumétrique de Hirst
Ta slovenski standard je istoveten z: CEN/TS 16868:2015
ICS:
13.040.20 Kakovost okoljskega zraka Ambient atmospheres
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

CEN/TS 16868
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
November 2015
TECHNISCHE SPEZIFIKATION
ICS 13.040.20
English Version
Ambient air - Sampling and analysis of airborne pollen
grains and fungal spores for allergy networks - Volumetric
Hirst method
Air ambiant - Échantillonnage et analyse des grains de Außenluft - Probenahme und Analyse luftgetragener
pollen et des spores fongiques aériens pour les réseaux Pollen und Pilzsporen für Allergienetzwerke -
aérobiologiques - Méthode volumétrique de Hirst Volumetrische Hirst-Methode
This Technical Specification (CEN/TS) was approved by CEN on 15 September 2015 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 16868:2015 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 8
5 Sampling . 8
5.1 Equipment . 8
5.1.1 Apparatus . 8
5.1.2 Scrolling speed of support . 11
5.1.3 Impaction support . 11
5.1.4 Wind vane and rain shield . 13
5.1.5 Complete sampling system . 14
5.2 Operating procedure . 14
5.2.1 Preparation of the coating medium [12] . 14
5.2.2 Support preparation . 15
5.2.3 Changing of the drum . 16
6 Analysis . 16
6.1 Equipment required . 16
6.2 Operating procedure . 17
6.2.1 Support . 17
6.2.2 Mounting medium . 17
6.3 Methodology for counting . 18
6.3.1 Glass slide preparation for microscopy analysis for drum tape [13] [14] . 18
6.3.2 Optical microscopy [14] [16] . 20
6.3.3 Identification [15] [16] . 20
6.3.4 Counting method . 20
6.3.5 Data recording . 20
6.3.6 Conversion factor . 21
7 Performance characteristics [10] [11] [17] [18] [19] . 22
7.1 General . 22
7.2 Performance requirements . 22
7.2.1 Repeatability . 22
7.3 Performance recommendations [18] [19] . 22
7.3.1 Reproducibility and accuracy . 22
7.3.2 Sensitivity and specificity. 22
Annex A (informative) Hirst type volumetric trap . 24
Annex B (informative) Pictures of impaction support. 25
Annex C (informative) Safety data sheet . 27
Annex D (informative) Key of determination . 28
Bibliography . 32
European foreword
This document (CEN/TS 16868:2015) has been prepared by Technical Committee CEN/TC 264 “Air
quality”, the secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Introduction
Biological particles (pollen and fungal spores) are present in the air, causing health impacts at various
levels. In Europe, nearly 18 % to 20 % of people suffer from pollinosis due to pollen and/or fungal
spores. Pollen grains and fungal spores are considered in some Member States as an air pollutant as
well as particles suspended in the air (PM , ). In Europe, European Aerobiology Society (EAS) in
10 2,5
coordination with International Association for Aerobiology (IAA) manage problems of sampling,
analysis, quality control, development and information.
For the sampling and analysis of biological particles different methodology and operating procedures
are used.
Sampling equipment is diversified (see Annex A). Analysis is based on optical light microscopy for
identification and counting pollen grains and fungal spores.
Elements and reagents used during sampling and analysis have very specific properties and require to
be handled carefully.
Given the close relationship between aerobiology and other sciences, one of the main aims is that
information on airborne biological-particle counts should be of use in a wide range of disciplines and
fields of application, including aerobiology, biodiversity, agriculture, forestry, phytopathology,
meteorology, climatology, forensic science, bioterrorism, and health (sensitization and allergy).
1 Scope
This European Standard specifies the procedure to sample continuously and analyse the concentration
of airborne pollen grains and fungal spores in ambient air using the volumetric Hirst type sampler [1]
[2] [3] (see Annex A).
This European Standard describes both the sampling and the analysis procedures for the purpose of
allergy networks. For the other tasks mentioned in the introduction, other specifications may be
required.
2 Normative references
Not applicable.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
accuracy
closeness of agreement between a measured quantity value and a true quantity value of a measurement
3.2
bench
long work table in a workshop or laboratory
3.3
clockwork
mechanism with a spring and toothed gearwheels, used to drive a mechanical clock, toy, or other device
3.4
combined standard measurement uncertainty
obtained using the individual standard measurement uncertainties associated with the input quantities
in a measurement model
3.5
defatted
surface conditions after clearing with a fat removing substance
3.6
drum
cylindrical device for the mounting of a sticky tape
3.7
exine
outer layer of the wall of a spore or pollen grain, also called an exosporium
3.8
eyepiece
lens or combination of lenses in an optical instrument through which the eye views the image formed
by the objective lens or lenses; ocular
3.9
flow meter
instrument for measuring the flow rate of a fluid in a pipe
3.10
flow rate
amount of fluid (e.g. air) that flows in a given time
3.11
fungal spore
reproductive unit capable of giving rise to a new individual with or without sexual fusion
3.12
hood
metal cover or canopy for a stove, ventilator, etc
3.13
impaction
sampling of airborne particles by inertial separation on any surface (e.g. of an adhesive)
3.14
magnetic stirrer
object or mechanical device used for stirring something
3.15
magnification
magnifying power of an instrument, e.g. this microscope should give a magnification of about x 100
3.16
microscope
optical instrument having a magnifying lens or a combination of lenses for inspecting objects too small
to be seen or too small to be seen distinctly and in detail by the unaided eye
3.17
objectives
optics (in a telescope, microscope, camera, or other optical system), the lens or combination of lenses,
that first receive the rays from the object and form the image in the focal plane of the eyepiece, as in a
mic
...