EN ISO 18254-1:2016

SLOVENSKI STANDARD
01-julij-2016
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Textiles - Method for the detection and determination of alkylphenolethoxylates (APEO)
(ISO 18254:2016)
Textilien - Verfahren zum Nachweis und zur Bestimmung von Alkylphenolethoxylaten
(APEO) (ISO 18254:2016)
Textiles - Méthode de détection et de détermination des alkylphénols éthoxylés (APEO)
(ISO 18254:2016)
Ta slovenski standard je istoveten z: EN ISO 18254-1:2016
ICS:
59.080.01 Tekstilije na splošno Textiles in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 18254-1
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2016
EUROPÄISCHE NORM
ICS 59.080.01
English Version
Textiles - Method for the detection and determination of
alkylphenol ethoxylates (APEO) - Part 1: Method using
HPLC - MS (ISO 18254-1:2016)
Textiles - Méthode de détection et de détermination
des alkylphénols éthoxylés (APEO) - Partie 1: Méthode
utilisant la CLHP-SM (ISO 18254-1:2016)
This European Standard was approved by CEN on 2 January 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18254-1:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 18254-1:2016) has been prepared by Technical Committee ISO/TC 38
"Textiles" in collaboration with Technical Committee CEN/TC 248 “Textiles and textile products” the
secretariat of which is held by BSI.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2016, and conflicting national standards shall
be withdrawn at the latest by October 2016.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands,
Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 18254-1:2016 has been approved by CEN as EN ISO 18254-1:2016 without any
modification.
INTERNATIONAL ISO
STANDARD 18254-1
First edition
2016-04-15
Textiles — Method for the detection
and determination of alkylphenol
ethoxylates (APEO) —
Part 1:
Method using HPLC-MS
Textiles — Méthode de détection et de détermination des alkylphénols
éthoxylés (APEO) —
Partie 1: Méthode utilisant la CLHP-SM
Reference number
ISO 18254-1:2016(E)
©
ISO 2016
ISO 18254-1:2016(E)
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved

ISO 18254-1:2016(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Principle . 1
3 Reagents . 1
4 Apparatus . 2
4.1 Apparatus and auxiliaries for preparing the sample . 2
4.2 Chromatographic equipment . 2
5 Procedure. 2
5.1 Standard preparation . 2
5.2 Sample preparation . 3
5.3 Analysis. 3
6 Calculation of results . 3
6.1 Determination of the R value for each congener of APEO . 3
6.2 Calibration curve . 3
6.3 Calculation of concentration of APEO result . 4
7 Test report . 4
Annex A (informative) Examples of chromatographic method – LC/MS . 5
Annex B (informative) Examples of chromatographic method – LC/MS/MS .8
Bibliography .10
ISO 18254-1:2016(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical
Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information.
ISO 18254-1 was prepared by the European Committee for Standardization (CEN) in collaboration with
ISO Technical Committee ISO/TC 38, Textiles, and Technical Committee CEN/TC 248, Textiles and textile
products in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna
Agreement).
ISO 18254 consists of the following parts under the general title Textiles — Method for the detection and
determination of alkylphenol ethoxylates (APEO):
— Part 1: Method using HPLC-MS
The following part is under preparation:
— Part 2; Method using NPLC
iv © ISO 2016 – All rights reserved

ISO 18254-1:2016(E)
Introduction
Alkylphenol ethoxylates (APEOs) are high-value products commonly used in industrial and consumer
detergents and cleaners, some plastics and many industrial applications. Their “down the drain” uses
may result in their presence in wastewater streams and receiving water bodies. Human exposure to
APEO can occur through diverse sources such as environmental, food, or skin contact. Considering their
toxicity on several animal species, minimization of exposure to APEO is recognized as important to the
preservation of human health.
Nonylphenol ethoxylates belong to the non-ionic surfactant category and are of particular concern.
The biodegradation of nonylphenol ethoxylate releases the branched nonylphenol, which is difficult to
biodegrade. Nonylphenol is a substance having endocrine disruptive properties that can have serious
effects on aquatic and many other organisms. For this reason, the release of nonylphenol ethoxylate
into the environment should be avoided.
Chemical products containing nonylphenol and/or nonylphenol ethoxylates in concentrations equal to
or greater than 0,1 % are restricted within the EU for specific uses, among others, the processing of
leather and textiles, industrial, and institutional cleaning.
This restriction is part of the entry 46 of Annex XVII of the REACH regulation EU 1907/2006, which
repealed the former Directive 2003/53/EC.
The current restriction is due to be widened to apply to textile products that can be washed in water. A
limit value of 0,01 % (100 ppm) is expected.
INTERNATIONAL STANDARD ISO 18254-1:2016(E)
Textiles — Method for the detection and determination of
alkylphenol ethoxylates (APEO) —
Part 1:
Method using HPLC-MS
SAFETY PRECAUTIONS — It is the user’s responsibility to use safe and proper techniques in
handling materials in this test method. Consult manufacturers for specific details such as
material safety, data sheets, and other recommendations. Good laboratory practice should be
followed. Users should comply with any national and local safety regulations.
1 Scope
This part of ISO 18254 descri
...