prEN 46-2

SLOVENSKI STANDARD
01-december-2025
Biocidni proizvodi za zaščito lesa - Ugotavljanje preventivnega delovanja proti
hišnemu kozličku Hylotrupes bajulus (Linnaeus) - 2. del: Ovicidni učinek
(laboratorijska metoda)
Wood preservatives - Determination of the preventive action against recently hatched
larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber frisch
geschlüpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung
(Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'action préventive contre les larves
récemment écloses d'Hylotrupes bajulus (Linnaeus) - Partie 2 : Effet ovicide (Méthode
de laboratoire)
Ta slovenski standard je istoveten z: prEN 46-2
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 71.100.50 Will supersede EN 46-2:2016
English Version
Wood preservatives - Determination of the preventive
action against recently hatched larvae of Hylotrupes
bajulus (Linnaeus) - Part 2: Ovicidal effect (Laboratory
method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'action préventive contre les larves récemment écloses Wirkung gegenüber frisch geschlüpften Larven von
d'Hylotrupes bajulus (Linnaeus) - Partie 2 : Effet ovicide Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung
(Méthode de laboratoire) (Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 46-2:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Test materials . 7
5.1 Biological material . 7
5.2 Products and reagents . 7
5.3 Apparatus . 7
6 Sampling . 8
7 Test specimens . 9
7.1 Species of wood. 9
7.2 Wood quality . 9
7.3 Provision of test specimens . 9
7.4 Dimensions of test specimens . 9
7.5 Number of test specimens. 9
7.5.1 Test specimens for egg-laying . 9
7.5.2 Test specimens for checking the tunnelling ability and the mortality of the
larvae . 10
8 Procedure . 10
8.1 Preparation of the test specimens . 10
8.1.1 Conditioning of the test specimens prior to sealing . 10
8.1.2 Sealing of test specimen faces . 10
8.1.3 Treatment of the test specimens . 11
8.1.4 Drying and conditioning of the test specimens after treatment . 12
8.2 Exposure of the test specimens to the insects (ovicidal action) . 12
8.3 Validity of the test . 13
9 Expression of results . 13
9.1 Ovicidal test . 13
9.2 Tunnelling control . 14
9.3 Total mortality . 14
10 Test report . 14
Annex A (informative) Example of a test report . 16
Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) . 19
B.1 General . 19
B.2 Obtaining parent beetles . 19
B.3 Mating . 19
B.4 Egg-Iaying . 19
B.5 Hatching of eggs . 20
B.6 Larval development . 20
B.7 Enemies and parasites . 21
Annex C (informative) Differentiation of heartwood and sapwood in Pinus species . 22
C.1 Principle . 22
C.2 Reagents . 22
C.3 Apparatus . 22
C.4 Procedure . 22
Annex D (informative) Environmental, health and safety precautions within
chemical/biological laboratory . 23
Bibliography . 24

European foreword
This document (prEN 46-2:2025) has been prepared by Technical Committee CEN/TC 38
“Biological durability of wood and wood-based products”, the secretariat of which is held by SIS.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 46-2:2016.
Significant technical differences between this document and EN 46-2:2016 are as follows:
a) specifications for sealing faces of test specimens to be treated with solutions in which an
organic solvent is the continuous phase were generalized (5.2.2);
b) requirements for temperature and humidity were aligned with other insect test standards
(5.3.1; 5.3.4; B5; B6);
c) imaging facilities for non-destructive recordings of insect tunnels inside test specimens
were generalized (5.3.11);
d) storage limitations for wood from which test specimens are to be made were deleted (7.2);
e) specifications for dipping (8.1.3.3) were generalized and aligned with other insect
standards.
NOTE Test results obtained according to earlier versions of this document or when the tests had
started before this version of EN 46-2 was published are considered as valid.
The standard EN 46 is composed of two parts:
— EN 46-1, Wood preservatives ― Determination of the preventive action against recently
hatched larvae of Hylotrupes bajulus (Linnaeus) ― Part 1: Application by surface treatment
(laboratory method)
— EN 46-2, Wood preservatives ― Determination of the preventive action against recently
hatched larvae of Hylotrupes bajulus (Linnaeus) ― Part 2: Ovicidal effect (laboratory method)
EN 46 consists of two parts to enable preventive action of wood preservatives, against recently
hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part
1 is required to determine the larvicidal effect of preservatives and Part 2 is required to
determine the ovicidal action of the preservatives after egg-laying of young females.

Introduction
This document describes a laboratory method of test which gives a basis for the assessment of
the preventive action of a wood preservative, when applied as a surface treatment for timber,
against eggs of Hylotrupes bajulus.
In combination with EN 46-1 it provides a means of checking whether larvae may hatch from
eggs laid on the treated wood surface and whether they are capable of boring through the
treated surface and of surviving in the untreated part of the wood.
This document provides information for the sealing of all but one lateral face when specimens
are to be treated by dipping.
When products which are very active at very low concentration are used, it is very important to
take suitable precautions to isolate and separate, as far as possible, operations involving
chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable
precautions should include the use of separate rooms, areas within rooms, extraction facilities,
conditioning cha
...